• 제목/요약/키워드: Cultivation independent method

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Production and Quality Parameters of Oat Grown in Conventional/Organic Farming

  • Petr Konvalina;Ivana Capouchova
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2022년도 추계학술대회
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    • pp.19-19
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    • 2022
  • Hulled and naked oat is a perspective crop for the low input production systems due to its low requirements for soil quality and nutrition. Oats have good competitive ability against weeds and can provide appropriate yield in organic farming in comparison with other cereal species such as wheat or barley. It is a perspective crop from the point of view of use in the food industry too. The aim of our study was to compare the production and quality parameters of naked and hulled oat grown in both organic (OF) and conventional fields (CF). Small plot trials were conducted in two locations in the Czech Republic (České Budějovice, Prague) for four years (2018-2021) in two production systems (OF, and CF). We used four varieties of hulled oat (Korok, Kertag, Raven, Seldon) and one variety of naked oat (Patrik). During the vegetation, agronomically important data were recorded. After harvest samples were processed in the laboratory and analyzed selected quality parameters of grain dry matter (the protein content was determined by the Kjeldahl method, starch content in grain according to Ewers, fat content in grain dry matter by the modified method according to Soxhlet, and ash content in grain dry matter). The data were evaluated using the program STATISTICA version 13.2, StatSoft, Inc., California, USA. It is clear from the results that the number of panicles before the harvest was influenced by the location, cultivation system, year, and, to a lesser extent, the influence of the variety. The number of panicles in OF averaged 340 per square meter, which was 90% of the value of CF. For thousand grain weight (TGW), a significantly predominant effect of year was found. The independent effect of location on TGW was statistically not significant. Grain yield was predominantly influenced by cultivation system and location. In OF, it reached an average of 3.97 t.ha-1, which was 75% of the yield of CF. As part of the evaluation of the basic grain quality indicators, the content of protein, starch, fat, and ash in the dry matter of the grain was evaluated. The content of protein in the dry matter of the grain was predominantly influenced by year, followed by the influence of the variety and a fairly comparable influence of the cultivation system and locality. On average, it achieved 16.05% in OF and 17.01% in CF. The starch content was then related to the protein content, where as a result of the lower protein content in the grain of OF oats, the content of starch and fat was on the contrary increased. The year turned out to be the most significant factor, affecting both the starch content in the dry matter of the grain and the fat content. This was followed again by a fairly comparable influence on the cultivation system and locality. The influence of the cultivation system and location was not statistically significantly applied in the case of ash content in dry matter. Based on our results we can propose both types of oat (hulled and naked) as perspective crops for OF. An organic farmer can expect to achieve stable yields which, in less favorable conditions for the production of cereals in the OF, may be close to the level of conventional yields. In the future, it will be important to change agrotechnology in OF and increase oat yield because this crop has a good potential to grow in areas with low nitrogen input or less fertile soil.

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에너지 효율 향상을 위한 스마트팜 제어 시스템 (Smart Farm Control System for Improving Energy Efficiency)

  • 최민석
    • 디지털융복합연구
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    • 제19권12호
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    • pp.331-337
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    • 2021
  • 정보통신기술과 융합된 스마트팜의 도입은 농업 분야의 생산성을 높이고 경쟁력을 강화하고 있다. 각종 센서를 통한 환경 모니터링과 이를 통한 재배 환경의 자동제어가 가능하며 원격제어를 지원하는 기술들이 개발되어 보급되었고, 스마트팜에서 생성된 데이터를 이용하여 스마트팜 기술의 고도화를 위한 연구들이 진행되고 있다. 본 논문에서는 스마트팜의 환경 및 제어 데이터를 이용하여 스마트팜의 에너지 소비를 줄이기 위한 환경 제어 방법을 제안한다. 누적된 환경 데이터를 이용하여 환경 예측 모델을 만들고, 다중 환경 요소를 고려하여 주어진 상황에서 에너지 소비를 최소화할 수 있는 제어 방식을 선택함으로써 독립적 환경 제어 방식과 비교해 에너지 사용량을 줄일 수 있음을 확인하였다. 향후 예측 모델의 고도화와 복합제어 알고리즘의 개선 통하여 더 높은 에너지 효율을 얻기 위한 연구가 필요할 것으로 보인다.

Tissue Culture of Stone Fruit Plants Basis for Their Genetic Engineering

  • Csanyi, Marta;Wittner, Anita;Nagy, Agenes;Balla, Ildiko;Vertessy, Judit;Palkovics, Laszlo;Balazs, Eevin
    • Journal of Plant Biotechnology
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    • 제1권2호
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    • pp.91-95
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    • 1999
  • Genetic engineering of stone fruit species like apricot, plum, peach and cherry are hampered by the inefficient and low-level regeneration processes in tissue culture. The first transgenic stone fruit species have emerged from transformed hypocotyls. These great achievements were applauded by the scientific community contrary the fact that hypocotyl derived transgenic plants have no real brooding value. Tissue culture of different organs of valuable cultivars are recorded with an extremely low-level of regeneration in the literature. To improve the tissue culture basis of stone fruit plants an extensive tissue culture programme were launched and dozens of different media were compared including a series of hormone concentration in the tissue culture systems. Our continuous efforts were crowned by a very efficient method for achieving up to 30-40% regenerable petioles. Usually on a single petiole several well-separated meristems were induced. After 3-4 weeks of cultivation shoots were developed. The basic media $K_2$ were supplemented with 10g/L saccharose, 10g/L glucose and 10g/L maltose. The following plant hormones were used BAP 1mg/L, TDZ 1mg/L, 2-iP 1mg/L and IAA 0,1 mg/L concentrations. The Petri dishes were kept for 3 weeks in dark at a temperature 22$^{\circ}C$ for 8 hours and 22-24$^{\circ}C$ for 16 hours. The Petri dishes were sealed with Parafilm. The regeneration of the petioles were genotype independent and we were able to regenerate different plum cultivars with almost the same efficiency.

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미생물 다양성 분석을 위한 웹기반의 생물정보도구 개발 (Web-based Research Assistant Tools for Analysis of Microbial Diversity)

  • 강병철;김현진;박준형;박희경;김철민
    • 한국지능시스템학회논문지
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    • 제14권5호
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    • pp.545-550
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    • 2004
  • 생태학, 환경공학, 임상진단 등 생물학 분야에서 미생물의 다양성 연구의 중요성이 대두되고 그 연구가 점증하고 있다. 특히 16S rRNA를 분자지표로한 DNA 염기서열 분석방법이 널리 사용되고 있다. 본 논문에서는 16S rRNA의 염기서열 분석과정을 각 단계별로 자동화하고, 생물학자들의 결과 판단이나 사용상의 편의를 도모하기 위하여 웹기반의 미생물 다양성 분석 어플리케이션을 개발하였다. 이를 위하여 단계별 자동화 및 인터페이스 개발에 적합한 폴더-프로세스-필터 모델을 고안하고 적용하였다. 제공되는 생물정보분석도구는 서열입력, 서열방향교정, 다중서열정렬 및 가시화, 서열동정 등의 분석이 있으며, 각 결과는 계통분류도구와 호환 가능하도록 하였다. 또한 신생아의 장내 세균총에 대한 분석을 수행하여 개발된 도구의 유용성을 확인하였다. 개발된 웹 어플리케이션은 리눅스 시스템 상에서 Perl 과 CGI를 이용하였으며, http://home.pusan.ac.kr/~genome/tools/rat.htm으로 접속하여 사용할 수 있다.

한국의 약용작물 생산 및 수·출입 동향과 개선방안에 관한 연구 (The study for the production of Korean medical crops and the export-import movement and the improving methods of them)

  • 김민희
    • 대한본초학회지
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    • 제32권2호
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    • pp.1-16
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    • 2017
  • Objective : The study was for researching the activation methods about Korean overall medical crops business from 2010 to 2016. In addition, it was to be given to examining complement points and improving methods to the changeable domestic market of medical crops caused by a considerable export-import amount of them. Methods : By examining and analyzing data for the trend of medical crops production and their export-import trend from 2010 to 2015, I found it valuable to use it as the basic resources for researching the urgent problem of medical crops' business and its improvement. Results : 1. As surveying the trend of the domestic medical crops over the recent six years, I found that the export amount of them has drastically decreased, while the import amount has increased. And so, the prices of the domestic medical crops and their production are unstable. 2. By developing standardized medical crops which a trading counterpart country could prefer, we have to promote the export competitiveness. 3. We should reinforce an origin mark and geographical mark practice for domestic herbal materials and expand GMP. 4. We should prepare a standardization of herbal medicines by setting up an independent governmental department. Conclusion : 1. The government should present an appropriate supply-demand amount of the medical crops depending on the domestic needs and the export-import transactions. 2. There should be an institutional supportive system which is able to guarantee stable incomes of farmers through contract cultivation. 3. We should develop high value standardized medical crops.

Genomic and Proteomic Analysis of Microbial Function in the Gastrointestinal Tract of Ruminants - Review -

  • White, Bryan A.;Morrison, Mark
    • Asian-Australasian Journal of Animal Sciences
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    • 제14권6호
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    • pp.880-884
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    • 2001
  • Rumen microbiology research has undergone several evolutionary steps: the isolation and nutritional characterization of readily cultivated microbes; followed by the cloning and sequence analysis of individual genes relevant to key digestive processes; through to the use of small subunit ribosomal RNA (SSU rRNA) sequences for a cultivation-independent examination of microbial diversity. Our knowledge of rumen microbiology has expanded as a result, but the translation of this information into productive alterations of ruminal function has been rather limited. For instance, the cloning and characterization of cellulase genes in Escherichia coli has yielded some valuable information about this complex enzyme system in ruminal bacteria. SSU rRNA analyses have also confirmed that a considerable amount of the microbial diversity in the rumen is not represented in existing culture collections. However, we still have little idea of whether the key, and potentially rate-limiting, gene products and (or) microbial interactions have been identified. Technologies allowing high throughput nucleotide and protein sequence analysis have led to the emergence of two new fields of investigation, genomics and proteomics. Both disciplines can be further subdivided into functional and comparative lines of investigation. The massive accumulation of microbial DNA and protein sequence data, including complete genome sequences, is revolutionizing the way we examine microbial physiology and diversity. We describe here some examples of our use of genomics- and proteomics-based methods, to analyze the cellulase system of Ruminococcus flavefaciens FD-1 and explore the genome of Ruminococcus albus 8. At Illinois, we are using bacterial artificial chromosome (BAC) vectors to create libraries containing large (>75 kbases), contiguous segments of DNA from R. flavefaciens FD-1. Considering that every bacterium is not a candidate for whole genome sequencing, BAC libraries offer an attractive, alternative method to perform physical and functional analyses of a bacterium's genome. Our first plan is to use these BAC clones to determine whether or not cellulases and accessory genes in R. flavefaciens exist in clusters of orthologous genes (COGs). Proteomics is also being used to complement the BAC library/DNA sequencing approach. Proteins differentially expressed in response to carbon source are being identified by 2-D SDS-PAGE, followed by in-gel-digests and peptide mass mapping by MALDI-TOF Mass Spectrometry, as well as peptide sequencing by Edman degradation. At Ohio State, we have used a combination of functional proteomics, mutational analysis and differential display RT-PCR to obtain evidence suggesting that in addition to a cellulosome-like mechanism, R. albus 8 possesses other mechanisms for adhesion to plant surfaces. Genome walking on either side of these differentially expressed transcripts has also resulted in two interesting observations: i) a relatively large number of genes with no matches in the current databases and; ii) the identification of genes with a high level of sequence identity to those identified, until now, in the archaebacteria. Genomics and proteomics will also accelerate our understanding of microbial interactions, and allow a greater degree of in situ analyses in the future. The challenge is to utilize genomics and proteomics to improve our fundamental understanding of microbial physiology, diversity and ecology, and overcome constraints to ruminal function.

실험대학 과제도서실 운영에 관한 조사연구 (A Research Survey on the Reserved Book System of Pilot Universities in Korea)

  • 최달현
    • 한국도서관정보학회지
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    • 제5권
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    • pp.119-168
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    • 1978
  • This is a survey of the reserved book system in the pilot universities in Korea. We have surveyed only 22 university libraries among 29 pilot schools as of 1977, because of the differences in the library users, library organization, library facilities, and library materials between universities and colleges. In 1972, the Korean Ministry of Education developed a reformation plan for their higher education based on the teaching method of curriculum-oriented faculty instead of that of the faculty-oriented curriculum. The former puts emphasis on the cultivation of a student's thinking, creativity, and judgement through self-teaching to do a given assignment. The reserved book system in a college or university library is one of the most important methods necessary to accomplish the above educational aim. The survey used a questionnaire with 50 question on 28 items concerning the various aspects of the reserved book system in 22 pilot universities. the survey result discovered many problems needing correction. The following list describes the measures needed to correct the problems found in the pilot universities. 1. The management of a centralized reserved book system is much more effective and economical than the decentralized reserved book system when a university is located on the same campus. 2. In the university library, an independent reserved book department requires to gain the desired educational aims as compared with the reserved book room controlled by any other department in the library. 3. The reserved book system should not be adopted by all the departments at once but enlarged gradually, for it needs the understanding and support of faculty members and the university itself. 4. As competence is essential to the effective operation of the reserved book room, the university library should not place an unqualified person in charge of the reserved book department. 5. The librarian in charge of the reserved book department is required to do more professional works such as analysis of users, collection and analysis of syllabuses, maintenance of faculty member cooperation, establishment of measures to acquire unavailable materials, and drawing up an effective management plan. However, he is spending most of his time in clerical works, that is, non-professional works. 6. Three to five titles of each reserved book are considered reasonable and required materials should be shelved in proportion to the number of students, that is, one copy per eight or ten students if the materials are allowed to lend for two hours at a time. For the supplementary materials, the library needs to place two or three copies per subject. 7. Professors must select reserved books with care so that they can be used year after year. 8. Few universities are asking professors the number of class students and the date when the reserved material will no longer be needed on reserve. 9. The library should gather all the lists of reserved books from every professor at least three to five months before the courses open, because it takes a long time to obtain foreign materials. 10. It is desirable that the reserved book department should collect the lists and prepare the materials with promptness and consistency. 11. Instead of block buying, it is desirable to purchase reserved books at the time the library gets the reserved book list from the professors. The library should also inform faculty members whether it obtained each reserved book or not before the course open. 12. The library should make a copy of materials if a professor requires to reserve an out-of-print book or partial contents of a book, journal, and thesis. 13. An independent budger for reserved books from the budget for general materials is desired. 14. The shelf arrangement of reserved books by courses or professors under the same department is much more preferable than a classified arrangement. 15. While most of the universities adopted the open shelves system for all the reserved books, it is more effective and economical to take a compromise system, that is, closed shelves for requires materials and open shelves for supplementary materials. 18. Circulation of reserved books needs a different system between required materials and supplementary materials: two or three hours and/or overnight loan for the former and two and/or three days loan for the latter. 17. A reserved book room should be open a long time after class so that students can have sufficient time to use the room. 18. The library must take daily and monthly statistic as well as statistics on every aspect of the reserved book system in order that the library ma decide on policy and management of the reserved book room in collaboration with the university. Furthermore, regular reports on the use of the reserved book room should be made to the president and the executive council by the library to acquire their understanding and cooperation for the reserved book system. 19. Cooperation of faculty members is indispensable to the effective management of the reserved book department and it is desirable to make a committee which will fix various decisions about the system. Whenever the director of the library make his decision, he must consult with his staff in order to involve them earnestly in the operation of the system.

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생산형 녹지 중 사과나무 과수원의 탄소흡수 및 배출 (Carbon Uptake and Emissions of Apple Orchards as a Production-type Greenspace)

  • 조현길;박성민;김진영;박혜미
    • 한국조경학회지
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    • 제42권5호
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    • pp.64-72
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    • 2014
  • 본 연구는 생산형 녹지로서의 과수원을 대상으로 사과나무에 의한 탄소의 저장 및 연간 흡수를 산정하고, 재배과정에서 관리에 기인하여 발생하는 연간 탄소배출량을 파악하여 탄소저감 효과를 계량화하였다. 연구대상 과수원에서 유목에서 성목에 이르는 일정 간격의 근원직경 크기를 고려한 수목을 구입하여, 근굴취를 포함하는 직접수확법에 의해 개체당 부위별 및 전체 생체량을 산정하고 탄소저장량을 산출하였다. 근원부의 줄기 원판을 채취하여 직경생장을 분석하고 연간 탄소흡수량을 산정하였다. 관리에 따른 연간 탄소배출은 전정, 제초, 관수, 시비, 병충해 방제 등을 포함하는 관리자료의 구득, 관리자 면담 및 부분 실측을 바탕으로 계량화하였다. 근원직경을 독립변수로 생장에 따른 사과나무 단목의 탄소저장과 연간 탄소흡수를 계량화하는 활용 용이한 계량모델을 개발하였다. 사과나무의 탄소저장량과 연간 탄소흡수량은 모두 직경생장과 더불어 증가하였고, 직경급 간 그 차이도 직경이 커질수록 증가하는 경향이었다. 근원직경 10 및 15cm인 사과나무 단목은 각각 9.1 및 21.0kg의 탄소를 저장하고, 연간 1.0 및 1.6kg의 탄소를 흡수하는 것으로 나타났다. 연구대상 과수원의 단위면적당 탄소저장량과 연간 탄소흡수량은 각각 3.81t/ha, 0.42t/ha/년이었고, 연간 탄소배출량은 1.30t/ha/년이었다. 즉, 관리 관련 연간 탄소배출량은 연간 탄소흡수량보다 약 3배 더 많았다. 연구결과를 토대로, 생산형 녹지에 적용 가능한 관수, 농약 및 비료의 효율적 적용을 포함하는 저탄소형 관리방안을 모색하였다. 본 연구는 아직 부진했던 뿌리 생체량의 실측과 탄소배출의 구체적 인벤토리를 통해 탄소계량 관련 정보를 구축하는 새로운 초석을 제공한다.

상산심학과 양명심학의 차별성 연구 - '송학'과 '명학'의 차별적 관점에서 - (The research about difference between Sangsan-Simhak and Yangming-Simhak - from a different point of view between 'Song-Hak' and 'Ming-Hak'-)

  • 이상호
    • 철학연구
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    • 제105권
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    • pp.321-350
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    • 2008
  • 이 논문은 '상산 심학'과 '양명 심학'의 차이를 '송학'과 '명학'이라는 차별성을 가지고 규명하려는 것이다. 이를 통해 상산학을 양명학의 전단계 학문정도로 이해하는 일반적 인식에서 벗어나 전체 성리학사에서 독자성을 가진 철학체계로 자리매김 시키려 했다. 이것은 전체 성리학사를 '리학'과 '심학'으로 이해하는 일반적 인식에서 벗어나, 시대적으로 변모된 철학적 패러다임에 근거해서 성리학의 전개를 이해하려는 것이기도 하다. 이와 같은 문제의식은 흔히 성리학을 리학과 심학으로 나누고, 전자를 정주학으로 후자를 육왕학으로 보는 일반적 인식으로부터 출발한다. 이렇게 되면서 상산학은 양명학의 전단계 학문으로만 인식되어, 전체 성리학사에서 독자적인 자리매김이 이루어지지 않았다. 또한 상산학은 주자학과는 철학적으로 대척점에 선 것으로 이해되면서, 이 둘 사이에는 이론적 동일성이 거의 없는 것으로 이해되기도 했다. 그러나 상산학은 '리학'적 패러다임이 일반화 되어 있던 '송학'의 배경 속에서 탄생되었으며, 양명학은 '기학'적 패러다임이 일반화 되어 있던 '명학'의 배경 속에서 탄생하였다. 이와 같은 '송학'과 '명학'의 차이는 실제 이들을 같은 학문으로 규정하게 하는 '심즉리心卽理'라는 명제에서도 의미 차이를 발생시킬 수밖에 없는데, 대부분의 연구는 바로 이 점을 간과하였던 것이다. 이를 위해 본 논문은 우선 '송학'과 '명학'의 철학적 차이점을 먼저 규명하고, 이것을 중심으로 상산학과 양명학을 같은 계통의 철학체계로 이해하게 했던 '심즉리'라는 명제가 가진 함의를 각각 분석하였다. 그리고 이와 같은 '심즉리'의 개념이 어떻게 수양론에 적용되는지를 확인함으로써, 상산학과 양명학의 철학 차이를 확인하고 있다. 특히 본 논문에서는 '리학'적 패러다임에 바탕한 '송학'과 '기학'적 패러다임에 바탕한 '명학'의 '리'의미 해석에 초점을 맞추어서, 여기에 대한 의미 차이가 '심즉리'의 의미 차이로 드러난다는 사실을 분명히 하고 있다. 이를 통해 궁극적으로 주자학과 상산학이 가지고 있는 '송학'적 패러다임의 특징과 양명학이 가지고 있는 '명학'적 패러다임에 차이가 있다는 사실을 논증하고 있으며, 여기에서 상산학은 양명학의 전단계 학문이 아니라, 그 자체의 체계성을 갖춘 성리학으로 자리매김 해야 한다는 입장을 밝히고 있다.