• Natural Product Sciences
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• 제28권4호
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• pp.181-186
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• 2022

A germacrane-type sesquiterpenoid was isolated and purified from a methanol extract of the roots of Valeriana fauriei (RVF) through open column chromatography using silica gel. This compound was verified to be heishuixiecaoline A by spectroscopic analysis. This compound was isolated for the first time from RVF. Quantitative analysis of heishuixiecaoline A from RVF cultivated from three different regions (Eumseong, Jinbu, and Jinan regions) was performed by combining high-performance liquid chromatography with a photodiode array detector. The extract of RVF cultivated in the Jinbu region showed the highest content (9.23 mg/g). In addition, a significant amount of the compound was detected in all RVF samples, which could be expected since it is a characteristic compound of RVF. The sesquiterpenoid group heishuixiecaoline A was isolated from RVF, a resource for various pharmacological substances, and quantitative analysis of RVF cultivated from three different regions was performed. As a result of these experiments, basic data on RVF that can be used in the development and application of pharmaceuticals and health functional foods in the future were obtained.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.215-215
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• 2022

Starch synthase proteins (SSI, SSII and SSIII) in rice are mainly involved in amylopectin synthesis mediating its chain elongation, and the functional loss of SSII can increase amylose accumulation through decreasing of amylopectin chain proportions. For purposes of identifying functional haplotypes and evolutionary analyses of this gene, SSII-1, we investigated 374 rice accessions belonging to different subgroups of origins. We subsequently performed bioinformatic analyses on their variations through haplotyping, resequencing and structuring based on different classified populations. Haplotyping of cultivated rice accessions using genetic variations within SSII-1 genomic region of chromosome 10 revealed a total of 8 haplotypes, representing 6 functional haplotypes by 4 non-synonymous SNPs of three different exons (1, 4 and 10), which effect on protein structure. Higher nucleotide diversity value was found in wild group (0.0055) compared to any of cultivated subpopulations, of which aus showed the most reduction of diversity value (0.0003). Tajima's D analysis exhibits the most Tajima's D value only in admixture group (0.3600) which appears to be the cause of a sudden population contraction by rare alleles scarcity. A clear separation of some wild accessions from the admixed cultivated subpopulations was observed in PCA and phylogenetic analysis. Similar admixed pattern of population structure was estimated with an increased K values of 2 to 8 where genetic components of almost all cultivated subpopulations were shared with the wild which can also be subsequently estimated by very low F_ST-values by -0.011 (wild-aromatic) and -0.003 (wild-admixture).

• Journal of Ginseng Research
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• 제41권3호
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• pp.326-329
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• 2017

Background: Cultivated ginseng is often introduced as a substitute and adulterant of Russian wild ginseng due to its lower cost or misidentification caused by similarity in appearance with wild ginseng. The aim of this study is to develop a simple and reliable method to differentiate Russian wild ginseng from cultivated ginseng. Methods: The mitochondrial NADH dehydrogenase subunit 7 (nad7) intron 3 regions of Russian wild ginseng and Chinese cultivated ginseng were analyzed. Based on the multiple sequence alignment result, a specific primer for Russian wild ginseng was designed by introducing additional mismatch and allele-specific polymerase chain reaction (PCR) was performed for identification of wild ginseng. Real-time allele-specific PCR with endpoint analysis was used for validation of the developed Russian wild ginseng single nucleotide polymorphism (SNP) marker. Results: An SNP site specific to Russian wild ginseng was exploited by multiple alignments of mitochondrial nad7 intron 3 regions of different ginseng samples. With the SNP-based specific primer, Russian wild ginseng was successfully discriminated from Chinese and Korean cultivated ginseng samples by allele-specific PCR. The reliability and specificity of the SNP marker was validated by checking 20 individuals of Russian wild ginseng samples with real-time allele-specific PCR assay. Conclusion: An effective DNA method for molecular discrimination of Russian wild ginseng from Chinese and Korean cultivated ginseng was developed. The established real-time allele-specific PCR was simple and reliable, and the present method should be a crucial complement of chemical analysis for authentication of Russian wild ginseng.

• 농업과학연구
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• 제49권2호
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• pp.203-214
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• 2022

A model is developed for estimating optimal cultivation areas for apples to maximize the total profit of apple farming by region, focusing on Gyeongsangbuk-do, Chungchungbuk-do, Gyeongsangnam-do, and Jeonllabuk-do in Korea. Comparing the current cultivation areas to the optimal areas according to the model estimation during the period 1999 - 2019, the former has exceeded the latter since 2015 in all regions except for Jeonllabuk-do. This result stems from a lack of the regulation of production quantity among apple producers' regional organizations. Accordingly, the decreasing rate of the market price was greater than the increasing rate of the production quantity, and the total profit of apple farming has fallen in conjunction with increasing agricultural wage rates. Therefore, in order to reverse the current decreasing trend in the profits of apple farming, it is necessary to regulate nationwide apple cultivation areas through regional producers' associations. Ex-ante forecasting for 2019 posits the following regional optimal cultivation areas for maximizing the total income from apple farming. The Gyeongbuk apple producers' association needs to reduce its current cultivation area by 1,089 ha and to maintain 18,373 ha. In the Chungbuk region, current cultivated area should be reduced by 1,027 ha to maintain 2,722 ha, and in the Gyeongnam region, the current cultivated area should be reduced by 582 ha to maintain 2,730 ha. In contrast, the Jeonbuk region needs to increase its current cultivation area by 174 ha and to maintain at a level of 2,872 ha.

• Mycobiology
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• 제39권4호
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• pp.299-300
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• 2011

Two species of cultivated Phellinus sp. were identified as P. baumii by internal transcribed spacer (ITS) sequence analysis. The fruit bodies of the examined strains were similar to those of naturally occurring strains, having a bracket-like form, yellow-to-orange color, and poroid hymenial surfaces. The DNA sequences of ITS region of both strains showed a homology of 99% with ITS1 to ITS2 sequences of P. (Inonotus) baumii strain PB0806.

• 디지털융복합연구
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• 제2권1호
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• pp.9-23
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• 2004

The purpose of this paper is to analyze quantitative and qualitative effects through input-output analysis and AHP(Analytic Hierarchy Process) method for checking the effects inducing high value to Jeju region economy by culture industry. In analysis results, Investment to culture industry has a positive effect on Income effect, employment effect, linking the other industry on Jeju region economy. And so, for activation of the Jeju region economy, Culture Industry based on Knowledge should be cultivated.

• 한국초지조사료학회지
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• 제43권2호
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• pp.95-102
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• 2023

본 연구는 국내 알팔파의 안정적인 생산을 위해 기존에 재배연구에서 수행한 재배기술을 조사하고 추가적으로 연구해야 될 연구를 제시하기 위해서 수행하였다. 연구에 사용한 자료는 1983~2008년까지 총 270점의 알팔파 재배실험 자료로 재배지역, 재배지, 품종, 파종, 수확횟수, 시비 및 건물수량 등을 수집하였다. 국내 알팔파의 건물수량은 12,536 kg/ha 으로 나타났으며 지역에 따른 차이가 큰 것으로 나타났다. 알팔파는 대부분 밭에서 재배가 되고 있었는데, 국내 알팔파 생산량을 높이기 위해서는 간척지 및 유휴지를 이용할 필요가 있으며 이를 개량할 토지개량제 연구가 필요하다. 국내에서 재배한 알팔파는 53개 품종이었으나 기존의 자료에서는 알팔파 품종선발 기준인 Fall dormancy를 고려한 연구는 전무하여 추가적인 연구가 필요하다. 시비는 각 비료의 성분을 다양한 수준으로 고려하지 않았으며 특히 알팔파 생산량의 증가를 고려할 때 K에 대한 연구가 필요하다. 알팔파의 수확은 지역에 따라 목초의 생육가능일수가 달라 수확횟수가 달랐으며, 수확횟수가 증가함에 따라 건물수량이 증가하는 경향을 보였다. 이는 우리나라에서 수확횟수에 따라 알팔파의 생산량을 높일 수 있을 것으로 생각되어 적정수준의 수확횟수를 제시하기 위한 연구가 필요하다.

• 한국작물학회지
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• 제56권1호
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• pp.64-71
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• 2011

Health beneficial properties of soybean [Glycine max (L.) Merr.] isoflavones are well known. The objectives of this study were to determine and compare the isoflavone composition and concentrations of soybean varieties grown in different cultivated regions of Vietnam (i.e., upland and lowland). Total and individual isoflavone composition and concentrations were determined by high-performance liquid chromatography (HPLC). Total isoflavone concentrations varied from 1153 to $6604{\mu}g\;g^{-1}$ and averaging $3354{\mu}g\;g^{-1}$ across environments and varieties. In the lowland region, the highest total isoflavones concentration was observed in M103 cultivar ($5653{\mu}g\;g^{-1}$) and the lowest in VX9-3 ($1153{\mu}g\;g^{-1}$), whereas in the upland region the highest and lowest concentrations were in M103 ($6604{\mu}g\;g^{-1}$) and DT93 ($1938{\mu}g\;g^{-1}$), respectively. Across varieties, average total isoflavones concentration was higher in the upland than lowland region (3728 vs. $2980{\mu}g\;g^{-1}$). The malonylglucosides and acetylglucosides concentrations in upland soybean varieties were higher than those from the lowland region. Despite the presence of Genotype (G) x Environment (E) interactions, varieties with consistently high (M103) and low (VX9-3, DT93) isoflavone concentrations across environments were identified. This is the first report of isoflavones in Vietnamese soybean varieties, revealing large variation in isoflavones concentration and profile among different varieties and cultivated regions. Results will be useful in selecting high-isoflavones soybean varieties for growth in tropical regions.

• 한국식품위생안전성학회지
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• 제4권3호
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• pp.191-198
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• 1989

고속 액체크로마토그래피를 이용하여 고추 중의 주요 신미성분인 Capsaicin 및 Dihydrocapsaicin을 분석하기 위한 최적 조건을 검토하고 고추의 품종, 재배지역 및 건조 방법에 따른 함량의 차이를 비교 검토하였다. 시료중의 Capsaicin 및 Dihydrocapsaicin을 70% ethanol, $60^{\circ}C$에서 1시간 동안 처리함으로써 효과적으로 추출할 수 있었으며 재현성 및 회수율시험 결과 분석데이타의 해석은 피이크의 면적을 기준으로 하는 것이 바람직함을 알수 있었다. 고추의 품종, 재배지역 및 건조 방법에 따라 함량의 차이가 큰 것으로 나타났으며 특히 건조방법에 의한 차이가 더욱 현저하여 태양건조의 경우가 기계건조의 경우에 비하여 capsaicin의 경우는 최고 80%, dihydrocapsaicin의 경우는 최고 60% 정도 높게 나타났다.

• Asian-Australasian Journal of Animal Sciences
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• 제21권12호
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• pp.1696-1702
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• 2008

In order to learn the origin of the Chinese Mongolian horse, we analyzed polymorphisms within the mtDNA D-loop variable region in 305 horses of 6 types of 3 different breeds, including one imported breed, one cultivated breed and 4 types of one local breed. We detected 13 different haplotypes, and subsequent sequence analysis showed that all 6 horse types were genetically diverse. By constructing a cladogram of mtDNA D-loop sequences from the 6 horse types along with homologous sequences from several other horse types obtained from GenBank, we showed that Chinese Mongolian horses have a close genetic relationship with other horse types from Mongolia. We also speculate that several Chinese Mongolian horses descended from Przewalskii horse. Additionally, the 13 haplotypes were dispersed throughout the cladogram, suggesting that Chinese Mongolian horses likely originated from multiple female ancestors. A phylogenetic map of the 6 horse types showed that the genetic relationship between the local Wuzhumuqin and Wushen types were the closest. The Xinihe and Baerhu were also closely related to each other, and slightly more distantly related to the cultivated Sanhe breed. All five of the local Chinese horse types had a much more distant relationship with the imported Thoroughbred breed.