• Title/Summary/Keyword: Cultivated Porphyra

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SPECIES OF CULTIVATED PORPHYRA IN KOREA (한국산 양식김의 종류)

  • KANG Jae Won
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.3 no.2
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    • pp.77-92
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    • 1970
  • Ueda, in the course of his systematic work on the lavers, Porphyra, in Japan and Korea in 1932, mentioned that most of the cultivated Porphyra belong to Porphyra tenera Kjellman. Then he, dividing the species into two forms, f. typica and f. kjellmani, put Korean cultivated Porphyra under the latter. From the 1930s to the early 1940s, Fujikawa, Kaneko and others worked on Physiological experiments or cultivational experiments of Porphyra in the culture-bed, but there was no mention about the cultivated Porphyra species. However, many fishermen generally recognize that the characteristics of cultivated Porphyra vary depending on their habitat or the picking season, and it is considered that these differences are due to the varieties of the species which are well adaptable to various environments. Recently, I have become aware of the predominant occurrence of P. yezoensis Ueda in most culture-beds of Korea as in the Tokyo Bay or other places in Japan. At present, since artificial seeding for the cultivation of Porphyra with Conchocelis has been carried out and peculiar species can be cultured, a study of the species of cultivated Porphyra has become an important subject. I collected the specimens from a number of culture-beds which are located in the legions shown in fig. 1 from January, 1968 to May, 1970 and found that there are five species, P. tenera Kjellman, P. yezoensis Ueda, P. kuniedai Kurogi, P. seriata Kjellman and P. suborbiculata Kjellman. Among them, P. kuniedai was treated as a round-type, a form of P. tenera, by Kunieda (1939) and Tanaka (1952) and the occurrence of this form is generally recognized by most fishermen. At present, as mentioned above, the most dominant species of cultivated Porphyra is P. yezoensis but the cultivation of P. tenera is restricted to certain culture-beds or the early half of the cultivation period. P. kunieda appears as a mixed species throughout most of the culture-beds, particulary in the later half of the period, while when it was picked in January it appeared dominantly in a place such as Gum-Dang where the 'Bal', splitted bamboo piece mat, was settled during the last of September. This is the first seeding process. The latter two species, P. suborbiculata and P. seriata appear frequently but in small amounts in the later half of the period particulary in the western region of the southern coast. However, it can not be ascertained when P. yezoensis becomes predominant, because specimens have not been available up until recent years but the process can be described as follows: We commonly recognize the ecological characteristics of P. tenera as follows; First, the conchospores of the species develop earlier and the period of its discharge is shorter than those of P. yezoensis; second, the microscopical buds discharge neutral spores which develop into new buds directly and buds develop repeatedly through a short period. Consequently, according to such above ecological characteristics, the species can grow thick on the 'Bal' exclusively. However, buds may disappear when they are harmed by disease such a 'infection by certain parasites or by other unusual environmental conditions. Thus P. yezoensis are enabled to grow on the 'Bal' instead of the former species since they not only develop later than the former but also macroscopical fronds discharge the neutral spore throughout the period from October to May. Likewise, if any disease appears in the culture-bed ill the later half of the period, the former is more severely damaged than the latter because the former have less resistance to the disease than the latter. Thus fewer frond survive and fewer carpospores which are the origin of the next generation can be discharged. However the latter by their nature can continue growing until early summer. In the case of the culture-bed where the above phenomenon occurs repeatedly P. yezoensis gradually may become the dominant species among cultivated Porphyra. In support of the validity of this process we find that according to the description and the plate of Wada (1941), P. tenera, P. yezoensis and P. kuniedai grow together in the culture-bed at the mouth of the Nakdong River where P. yezoensis occurs predominantly and mixed with P. kuniedai.

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Color of Cultivated Porphyra and its Fading (Iro-ochi) and Recovery

  • Aruga, Yusho
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2001.10a
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    • pp.23-24
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    • 2001
  • Iro-ochi as has been called by Nori (Porphyra) farmers is biologically a bleaching phenomenon and has been one of the serious problems in Nori cultivation in Japan. Especially in the last Nori cultivation season, Iro-ochi gave an extremely serious damage to the Nori production in Ariake Bay, Kyushu, Japan, which attracted considerable socio-economic attention in Japan. As early as in late 1960s, we already made fundamental studies on Iro-ochi of Porphyra. (omitted)

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Comparative Photosynthetic Physiology of Fronds and Cultivated Filaments of Porphyra pseudolinearis Ueda (한국산 긴잎돌김 (Porphyra pseudolinearis Ueda)의 엽상체와 배양 사상체의 광합성 비교)

  • KIM Hyung-Geun;CHU Su-Dong;JUN Bang-Ook
    • Journal of Aquaculture
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    • v.7 no.1
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    • pp.1-7
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    • 1994
  • The effects of temperature and light intensity on the photosynthesis were investigated in fronds and cultivated filaments of Porphyra pseudolinearis Ueda. The optimum temperatures for total photosynthesis of fronds and filaments were $25\~30^{\circ}C\;and\;20^{\circ}C$, respectively. The photosynthetic rates of fronds and filaments based on light intensity were shown in a typical light saturation curve where the rates were constant over the light intensity of approximately 10,000 lux. The compensation points were 2,100 lux in fronds and 900 lux in filaments. The photosynthetic rate of filaments was 5 to 10 times greater than that of fronds. This would be very advantageous for filaments to conduct photosynthesis in the condition of dim light.

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DNA Sequences and Identification of Porphyra Cultivated by Natural Seeding on the Southwest Coast of Korea (한국 남서해안 자연채묘 양식 김의 DNA 염기서열과 종 동정)

  • Hwang, Mi-Sook;Kim, Sun-Mi;Ha, Dong-Soo;Baek, Jae-Min;Kim, Hyeung-Seop;Choi, Han-Gu
    • ALGAE
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    • v.20 no.3
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    • pp.183-196
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    • 2005
  • Nuclear SSU and ITS1 rDNA and plastid rbcL sequences were determined to identify the seven samples of Porphyra cultivated by means of natural seeding on the southwest coast of Korea and analyzed to access the phylogenetic relationships of them with the natural populations of P. tenera and P. yezoensis from Korea and Japan. SSU, rbcL and ITS1 data from 18, 21 and 31 samples, respectively, including previously published sequences were investigated in the study. Results from our individual and combined data indicated that the seven samples were all P. yezoensis and the entities except one from Muan 2 aquafarm strongly grouped together with the natural populations of P. yezoensis from the south and the west coast of Korea. The sample from Muan 2 seems to be derived from a strain of P. yezoensis introduced from Japan by Porphyra farmers, based on DNA sequence data.

Diseases of the cultivated Porphyra at seocheon area (서천 해역 김 양식장의 갯병에 관한 연구)

  • Lee, Soon Jeong;Park, Sung Woo;Lee, Jong Hwa;Kim, Young Sik
    • Journal of fish pathology
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    • v.25 no.3
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    • pp.249-256
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    • 2012
  • Disease occurrence and dead cell ratio of Porphyra cultivated at Seocheon area were studied. Seaweed samples were collected monthly at 12 cultivating sites of Porphyra from Nov. 2008 to Mar. 2009. Although disease occurrence was low at the beginning of cultivation at the most of collecting sites, the occurrence increased with time. The most common symptom of Porphyra disease in this area was diatom attachment, followed by white rot disease. Licmophora sp. was the most common species of diatom and its attachment was the highest on November, the beginning of cultivation. Although the occurrence ratios of green spot disease and white rot disease were high on November, chytrid disease was increased at the end of cultivation period. Dead cell ratio of Porphyra thalli was different at each study site and cultivating time, but normally the ratio was high at the end of cultivating season. The changes of dead cell ratio could be related to the nutrition supplement and currents, and the more precise reason of dead cell ratio would be revealed by further research related to cultivating environment conditions.

Model Plants in Marine Biotechnology

  • Saga, Naotsune;Endo, Hirotoshi
    • Journal of Marine Bioscience and Biotechnology
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    • v.4 no.1
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    • pp.11-14
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    • 2010
  • The genus Porphyra, consisting of 133 species includes several economically valuable species (i.e. P. yezoensis, P. tenera, P. pseudolinearis etc.). They are predominantly consumed and cultivated in East Asian countries such as Japan, Korea and China, and they are regarded as a big commercial market today. In addition to the industrial importance, P. yezoensis is currently regarded as a feasible candidate for a model plant in marine biotechnology, therefore there are a wide range of studies being undertaken: strain-preservation, development of mutant strains and genetic analysis and exhaustive molecular analysis including EST and macro/micro array. Focusing on the activities of our research group, current situation and future perspectives in marine biotechnological studies using P. yezoensis will be discussed in this mini review.

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Development of a tide-simulating apparatus for macroalgae

  • Kim, Jang-K.;Yarish, Charles
    • ALGAE
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    • v.25 no.1
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    • pp.37-44
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    • 2010
  • A tide-simulating apparatus was developed for culturing marine macroalgae. The objective of this study was to introduce a novel tide-simulating apparatus that can simulate a diurnal or semi-diurnal tidal cycle in the laboratory. In this apparatus, the seaweeds are move up and down and the water level remains the same during the simulated tidal cycle. The apparatus consists of 18 cylindrical culture tanks (3 blocks $\times$ 6 culture tanks) with 12 cm diameter and 24.5 cm long containing up to 2.5 L of seawater. There is a horizontal plate which covered all 18 culture tanks, and it is raised and lowered by a programmable motor that can regulate exposure time. In one application, seaweeds are attached to braided twine hung on Plexiglas air-tubing. The air-tubing is attached to a lid that is set on a horizontal plate. This apparatus is made of colorless Plexiglas to maximize light transmittance. This apparatus is easily disassembled and transportable to any indoor laboratory, wet laboratory, greenhouse, etc. This apparatus also offers considerable flexibility in terms of design. The size of culture tank can be redesigned by either increasing the height of cylinder or/and using a different diameter of cylindrical Plexiglas, therefore, larger/taller thalli can be cultivated. Growth rates of three eulittoral Porphyra species from different tidal elevations have been compared using this device.

DISEASES OF THE CULTTVATED PORPHYRA AT CULTURE BEDS WITH SPECIAL REFERENCE TO THE EFFECTS OF FERTILIZER PLANT EFFLUENTS (낙동강 하구 부근(용원리) 김 어장의 갯병 특히, 공장 폐수의 영향에 관하여)

  • KANG Jae Won
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.5 no.2
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    • pp.39-44
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    • 1972
  • Considerable damages of the cultivated Porphyra by Porphyra diseases were reported from the Porphyra culture bed along the coast of Yongwon-ri, Changwon, Kyungnam during the years of 1969 and 1970. The present study deals with the effects of fertilizer plant effluents on the Porphyra diseases, and the results are summarized as follows : 1. By the result of current observation, the polluted water of Haeng-am Bay which has an inflow of pollutants from the fertilizer plant was affecting the bed with tides. The results of pollution studies in Chinhae Bay and adjacent waters conducted by Won and Park(1971) also show that Chinhae Bay and adjacent waters are contaminated with the plant effluents. It seems that the effect increases due to the wind drift current when north-westerly or westerly winds prevail. Accordingly, effects of the Porphyra diseases in the culture bed seem to originate from the pollutants, since there are more damages when the north-westerly or westerly winds prevail, and also spring tide develops. 2. As compared to the photosynthetic activity of the Porphyra suborbiculata in uncontaminated seawater, it decreases up to $4\%$ in 200pmm, $20\%$ in 300ppm and $43\%$ in 1,000ppm of contaminated seawater which contains dilluted pollutants from the fertilizer plant. The results of the measurements using the water collected in the polluted area of Chinhae Bay and adjacent waters revealed that the photosynthesis was depressed about 21 to $34\%$ near the plant, and in the area of the Porphyra beds, $15\%$ in the portion where tide is weak and $5\%$ where the tide is strong, in comparison with the area of unpolluted water. 3. Although the present results do not indicate the exact level of harmful pollutants, it is evident that the pollutants of the plant effluents interfere photosynthetic activity of the Porphyra, even in the water containing pollutants as low as 200 ppm.

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A revaluation of algal diseases in Korean Pyropia (Porphyra) sea farms and their economic impact

  • Kim, Gwang Hoon;Moon, Kyoung-Hyoun;Kim, Je-Yoon;Shim, Junbo;Klochkova, Tatyana A.
    • ALGAE
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    • v.29 no.4
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    • pp.249-265
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    • 2014
  • As with land crops, cultivated algae are affected by various diseases ranging from large outbreaks of a disease to chronic epiphytes, which may downgrade the value of the final product. The recent development of intensive and dense mariculture practices has enabled some new diseases to spread much faster than before. A new disease is reported almost every year, and the impact of diseases is expected to increase with environmental change, such as global warming. We observed the incidence of diseases in two Pyropia sea farms in Korea from 2011 to 2014, and estimated the economic loss caused by each disease. Serious damage is caused by the oomycete pathogens, Pythium porphyrae and Olpidiopsis spp., which decreased the productivity of the Pyropia sea farms. In Seocheon sea farms, an outbreak of Olpidiopsis spp. disease resulted in approximately US $1.6 million in loss, representing approximately 24.5% of total sales during the 2012-2013 season. The damage caused by green-spot disease was almost as serious as oomycete diseases. An outbreak of green-spot disease in the Seocheon sea farms resulted in approximately US $1.1 million in loss, representing 10.7% of total sales in the 2013-2014 season in this area. However, the causative agent of green-spot disease is still not confirmed. "Diatom felt" is regarded as a minor nuisance that does not cause serious damage in Pyropia; however, our case study showed that the economic loss caused by "diatom felt" might be as serious as that of oomycete diseases. Bacteria and cyanobacteria are indigenous members of epiphytic microbial community on Pyropia blades, but can become opportunistic pathogens under suitable environmental conditions, especially when Pyropia suffers from other diseases. A regular acid wash of the Pyropia cultivation nets is the most common treatment for all of the above mentioned diseases, and represents approximately 30% of the total cost in Pyropia sea farming. However, the acid wash is ineffective for some diseases, especially for Olpidiopsis and bacterial diseases.

STUDIES ON THE EXTRACTION OF SEA WEED PROTEINS 3. Extraction of NaOH Soluble Proteins (해조단백질 추출에 관한 연구 3. NaOH 가용성 단백질의 추출)

  • LEE Kang-Ho;WOO Soon-Im;RYU Hong-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.11 no.2
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    • pp.85-90
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    • 1978
  • In present study, the effect of various factors including the solvent concentration, extraction time, extraction temperature and the ratio of sample vs extraction solvent(w/v) upon the extractability of the NaOH soluble proteins of marine algae were investigated. Seven species of sun-dried algae, the major ones in consumption as food, namely Porphyra suborbiculata, Undaria pinnatifida(natural and cultivated), Sargassum fulvellum, Sargassum kjellmanianum, Ulva pertusa, Enteromorpha linza and Codium coarctatum were used for the extraction of the NaOH soluble protein. The frozen and masceratd samples were prepared by the same mettled described in previous paper(Lee, 1977). In case of the TCA insoluble protein, all samples reached maxima at 0.025M NaOH solution while the 0.05M for extractable total nitrogen. Variation of the ratio of sample vs solvent gave slight effect upon the extractability, 100 ml solvent added to 1 g dried sample was effective. The effect of extraction time on the extractability differed from species. The extractabilty of Enteromorpha linza, Ulva pertusa and Codium coarctatum reached maxima within 1 hour extraction and 2 hours for the cultivated Undaria pinnatifida while 3 flours for the natural Undaria pinnatifida, Sargassum fulvellum, Sargassum kjellmanianum and Porphyra suborbiculata. The most effective extraction temperature was $60^{\circ}C$ for all samples.

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