• Journal of Plant Biotechnology
• /
• 제2권1호
• /
• pp.51-54
• /
• 2000

Leaf explants of the cucumber (Cucumis sativus L.) were cultured on Murashige and Skoog's (MS) medium supplemented with various concentrations of $\alpha$-naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). Direct shoot orgnogenesis as well as callus formation with somatic embryos and multiple shoots was observed from leaf explants of cvs. Shinhukjinju and Chungjang. The highest frequency of shoot formation 80% was observed on MS medium supplemented with NAA/BAP (5.0 ${\mu}{\textrm}{m}$/2.5 ${\mu}{\textrm}{m}$), with explants forming 3-7 shoots. Shoots formation occured within 3 to 4 weeks. Only one subculture of calli was required for plant regeneration on normal growth regulator-free medium. Plantlets transferred to soil developed into plants of normal appearance, which flowered and set fruits.

• 한국환경농학회지
• /
• 제9권2호
• /
• pp.143-151
• /
• 1990

배양액(培養液) 및 배지(培地)의 종류(種類)를 달리하여 수행(遂行)한 양액재배(養液栽培) 실험(實驗)에 있어서 오이(Cucumis sativus L.)와 토마토(Lycopersicon esculentum MILL)의 생육(生育)과 품질(品質)에 미치는 영향(影響)을 조사(調査)한 바 그에 대한 실험(實驗) 결과(結果)를 요약하면 다음과 같다. 1. 오이와 토마토의 생육(生育) 및 수량(收量)은 Cooper액(液)을 이용(利用)한 암선재배(岩線栽培)에서 가장 높게 나타났다. 2. 복합비료(複合肥料) 양액(養液)을 이용(利用)한 암선재배(岩線栽培) 시(時) 생육(生育) 및 수(收量)은 sand-sack culture에서 가장 양호(良好)하였다. 3. 복합비료(複合肥料) 양액(養液)을 사용(利用)한 암선재배(岩線栽培) 시(時) 초기생육(初期生育)에는 토양재배(土壤栽培)보다는 좋았으나 후기생육(後期生育)은 점차 낮아지는 결과(結果)를 나타내었다. 4. 오이와 토마토의 과실내(果實內) vitamin C함량(含量)은 토양재배(土壤栽培)와 비교(比較) 시(時) 복합비료양액(複合肥料養液)을 사용(利用)한 암선재배구(岩線栽培區)를 제외하고 나머지 처리구(處理區)에서는 차이가 없었다. 5. 오이 및 토마토 건물중(乾物重), total-N 및 무기성분(無機成分)의 함량(含量)에서 각 처리구별(處理區別) 뚜렷한 차이는 나타나지 않았다.

• 한국식물생명공학회:학술대회논문집
• /
• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
• /
• pp.185-185
• /
• 2005

• 생명과학회지
• /
• 제8권3호
• /
• pp.272-278
• /
• 1998

UV-B 강도 변화가 식물의 생장에 미치는 영향을 조사하기 위하여, 오이를 이용하여 3주간 3단계의 UV-B[일일 평균 UV-$B^{BE}$; 무처리(0.03), 저UV-B(6.40), 고UV-B(11.32) kJ $m^{-2}$]조사 실험을 수행하였다. 3주간의 UV-B 조사에 의해 건물중 및 엽면적은 고UV-B 처리와 저UV-B 처리에서 각각 60%와 40% 정도의 감소를 보였으나, 엽중비는 증가하였다. UV-B에 의해 고UV-B 처리에서 약 45%, 저UV-B 처리에서 약 37%의 광합성속도의 감소를 나타내었으나 증산속도의 변환느 나타나지 않았다. 총 엽록소 함량은 UV-B 강도의 증가에 따라 감소하였으며, UV-흡수물질인 flavonoid의 함량은 고UV-B 처리에서 약 19% 저UV-B처리에서 약 14%의 증가를 보였다. 이상의 결과로 볼 때 UV-B조사는 오이 식물에 극심한 생육 저해를 나타내었으며, 그 피해는 UV-B 강도에 따라 증가하였다. 따라서 현재 지구에 도달하는 태양UV-B에 의해서도 오이식물은 영향을 받고 있는 것으로 사료되며, 장래 예상되는 오존층 감소에 의해서도 생육장해를 나타낼 것으로 사료된다.

• 식물병연구
• /
• 제30권2호
• /
• pp.189-193
• /
• 2024

오이(Cucumis sativus L.)은 국내에서 경제적으로 중요한 작물이며, 지속 가능한 생산을 위해서는 다양한 식물병을 효과적으로 관리해야 한다. 본 연구에서는 이전의 연구에서 선발한 오이 탄저병 억제 Streptomyces sp. S20-465가 생성하는 대사산물을 동정하고, Colletotrichum orbiculare에 의한 오이 탄저병 방제에 대한 효과를 규명하였다. Streptomyces sp. S20-465는 YEME 배지에서 효과적으로 생장, 포자 형성 및 항진균 활성이 있었으며, 배양 여과액의 n-hexane 추출물에 함유된 4-hydroxy-4-methyl-2-pentanone이 오이 탄저병을 효과적으로 억제하였다. 본 연구 결과는 Streptomyces sp. S20-465 유래 대사산물이 오이 탄저병 등 식물병 관리에 효과적인 소재로 활용될 수 있음을 제시하며, 이는 오이 생산을 지속 가능하게 향상시키는 방안이 될 수 있을 것이다.

• 원예과학기술지
• /
• 제31권4호
• /
• pp.393-399
• /
• 2013

The effect of solution temperature and nitrogen form on cucumber (Cucumis sativus L.) growth, photosynthesis and nitrogen metabolism was investigated in hydroponic culture. Cucumber plants were grown for 35 days in a greenhouse at three constant solution temperatures ($15^{\circ}C$, $20^{\circ}C$, and $25^{\circ}C$) within a natural aerial temperature ($15-30^{\circ}C$). Four nitrate:ammonium ($NO{_3}^-:NH{_4}^+$) ratios (10:0, 8:2, 5:5, and 2:8 $mmol{\cdot}L^{-1}$) at constant nitrogen (N) concentration of $10mmol{\cdot}L^{-1}$ were applied within each solution temperature treatment. Results showed an increasing solution temperature enhanced plant growth (height, dry weight, and leaf area) in most N treatments. Dry weight accumulation was greatest at the 10:0 $NO{_3}^-:NH{_4}^+$ ratio in the $15^{\circ}C$ solution, the 5:5 ratio in the $20^{\circ}C$ solution and the 8:2 ratio in the $25^{\circ}C$ solution. Photosynthetic rate (Pn) response to solution temperature and $NO{_3}^-:NH{_4}^+$ ratio was similar to that of plant growth. Probably, the photosynthate shortage played a role in the reduced biomass formation. Increasing solution temperature enhanced the nitrate reductase (NR) activity, and further reduced shoots nitrate content. Our results indicate that the optimal ratio of nitrate to ammonium that promotes growth in hydroponic cucumber varies with solution temperature.

• 한국식물생명공학회:학술대회논문집
• /
• 한국식물생명공학회 2004년도 생명공학 실용화를 위한 비젼
• /
• pp.100-100
• /
• 2004

• 한국식물학회:학술대회논문집
• /
• 한국식물학회 1999년도 춘계학술발표대회:발표논문요지록
• /
• pp.13-13
• /
• 1999

• 한국유기농업학회지
• /
• 제3권1호
• /
• pp.79-85
• /
• 1994

Worm cast is one of the effective and the least harmful organic fertilizer. The purpost of this work is to elucidate the physiological role of worm cast in the plant growth. Worm cast extract contains 17.83ppm of zinc, but either cadmium or lead were not detected in it. Cucumber (Cucumis sativus L. cv. H. N. Semiwhite) was germinated and incubated with percentage-varied extract. While 10% extract stimulated sprouting of seeds, 100% extract had no effct on it. 50% extract and 100% extract enhanced hypercotyl elongation and fresh weight most effectively. Dry matter and zinc concentration in hypercotyl were increased as percentage of extract increased. Protein con-tent was not affected by extract, 100% extract inhibits water uptake of seedling. Considering these, enhancement of seedling growth was mainly based on increment of water uptake.

• 한국환경과학회지
• /
• 제16권12호
• /
• pp.1345-1353
• /
• 2007

The effect of salicylic acid(SA) on antioxidant system and protective mechanisms against UV-B induced oxidative stress was investigated in cucumber(Cucumis sativus L.) leaves. UV-B radiation and SA were applied separately or in combination to first leaves of cucumber seedlings, and dry matter accumulation, lipid peroxidation and activities of antioxidant enzymes were measured in both dose and time-dependant manner. UV-B exposure showed reduced levels of fresh weight and dry matter production, whereas SA treatment significantly increased them. SA noticeably recovered the UV-B induced inhibition of biomass production. UV-B stress also affected lipid peroxidation and antioxidant enzyme defense system. Malondialdehyde(MDA), a product of lipid peroxidation, was greatly increased under UV-B stress, showing a significant enhancement of a secondary metabolites, which may have antioxidative properties in cucumber leaves exposed to UV-B radiation. Combined application of UV-B and SA caused a moderate increase in lipid peroxidation. These results suggest that SA may mediate protection against oxidative stress. UV-B exposure significantly increased SOD, APX, and GR activity compared with untreated control plants. Those plants treated with 1.0 mM SA showed a similar pattern of changes in activities of antioxidant enzymes. SA-mediated induction of antioxidant enzyme activity may involve a protective accumulation of $H_2O_2$ against UV-B stress. Moreover, their activities were stimulated with a greater increase by UV-B+SA treatment. The UV-B+SA plants always presented higher values than UV-B and SA plants, considering the adverse effects of UV-B on the antioxidant cell system. ABA and JA, second messengers in signaling in response to stresses, showed similar mode of action in UV-B stress, supporting that they may be important in acquired stress tolerance. Based on these results, it can be suggested that SA may participates in the induction of protective mechanisms involved in tolerance to UV-B induced oxidative stress.