Browse > Article
http://dx.doi.org/10.5322/JES.2007.16.12.1345

Effects of Salicylic Acid on Oxidative Stress and UV-B Tolerance in Cucumber Leaves  

Hong, Jung-Hee (Dept. of Biology, Pusan National University)
Kim, Tae-Yun (Dept. of Biology, Pusan National University)
Publication Information
Journal of Environmental Science International / v.16, no.12, 2007 , pp. 1345-1353 More about this Journal
Abstract
The effect of salicylic acid(SA) on antioxidant system and protective mechanisms against UV-B induced oxidative stress was investigated in cucumber(Cucumis sativus L.) leaves. UV-B radiation and SA were applied separately or in combination to first leaves of cucumber seedlings, and dry matter accumulation, lipid peroxidation and activities of antioxidant enzymes were measured in both dose and time-dependant manner. UV-B exposure showed reduced levels of fresh weight and dry matter production, whereas SA treatment significantly increased them. SA noticeably recovered the UV-B induced inhibition of biomass production. UV-B stress also affected lipid peroxidation and antioxidant enzyme defense system. Malondialdehyde(MDA), a product of lipid peroxidation, was greatly increased under UV-B stress, showing a significant enhancement of a secondary metabolites, which may have antioxidative properties in cucumber leaves exposed to UV-B radiation. Combined application of UV-B and SA caused a moderate increase in lipid peroxidation. These results suggest that SA may mediate protection against oxidative stress. UV-B exposure significantly increased SOD, APX, and GR activity compared with untreated control plants. Those plants treated with 1.0 mM SA showed a similar pattern of changes in activities of antioxidant enzymes. SA-mediated induction of antioxidant enzyme activity may involve a protective accumulation of $H_2O_2$ against UV-B stress. Moreover, their activities were stimulated with a greater increase by UV-B+SA treatment. The UV-B+SA plants always presented higher values than UV-B and SA plants, considering the adverse effects of UV-B on the antioxidant cell system. ABA and JA, second messengers in signaling in response to stresses, showed similar mode of action in UV-B stress, supporting that they may be important in acquired stress tolerance. Based on these results, it can be suggested that SA may participates in the induction of protective mechanisms involved in tolerance to UV-B induced oxidative stress.
Keywords
Antioxidative enzyme; Cucumber(Cucumis sativus L.); Lipid peroxidation; Oxidative stress; Salicylic acid; UV-B;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Yang Y., Yao Y., Xu G., Li C., 2005, Growth and physiological responses to drought and elevated ultraviolet-B in two contrasting populations of Hippophae rhamnoides, Physiol. Plant, 124, 431-440   DOI   ScienceOn
2 Shinkle J. R., Atkins A. K., Humphrey E. E., Rodgers C. W., Wheeler S. L. Barnes P. W., 2004, Growth and morphological responses to different UV wavebands in cucumber(Cucumis sativus) and other dicotyledonous seedlings, Physiol. Plant, 120, 240-248   DOI   ScienceOn
3 Jasen M. A. K., 2002, Ultraviolet-B radiation effects on plants : induction of morphogenic responses, Physiol. Plant, 116, 423-429   DOI   ScienceOn
4 Mark U., Tevini M., 1997, Effects of solar ultraviolet-B radiation, temperature and $CO_2$ on growth and physiololgy of sunflower and maize seedlings, Plant Ecology, 128, 225-234   DOI
5 Larkindale J., Knight M. R., 2002, Protection against heat stress-induced oxidative damage in Arabidopsis involves calcium, abscisic acid, ethylene and salicylic acid, Plant Physiol., 128, 682-695   DOI   ScienceOn
6 Koda Y., 1997, Possible involvement of jasmonates in various morphogenetic event, Physiol. Plant, 100, 639-646   DOI   ScienceOn
7 Sharma Y. J., Leon J., Raskin I., Davis K. R., 1996, Ozone-induced responses in Arabidopsis thaliana : the role of salicylic acid in the accumulation of defense related transcripts and induced resistance, Proc. Natl. Acad. Sci. U.S.A., 93, 5099-5104
8 Harborne J. B., Williams C. A., 2000, Advances in flavonoid research since 1992, Phytochem., 55, 481-504   DOI   ScienceOn
9 Dat J. F., Lopez-Delgado H., Foyer C. H., Scott J. M., 2000, Effects of salicylic acid on oxidative stress and thermotolerance in tobacco, J. Plant Physiol., 156, 659-665   DOI   ScienceOn
10 Senaratna, T., Touchell, D., Bunn, T., Dixon, K., 2002, Acetylsalicylic acid (Aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plants, Plant Growth Regul., 30, 157-161   DOI   ScienceOn
11 De Gara L., Paciolla C., De Tullio M. C., Matto M., Arrigoni O., 2000, Ascorbate-dependent hydrogen peroxide detoxification and ascorbate regeneration during germination of highly productive maize hybrid : evidence of an improved detoxification mechanism against reactive oxygen species, Plant Physiol., 109, 7-13   DOI   ScienceOn
12 Kim, H. S., Lim, C. J., Han, T. J., Kim, J. C., Jin, C. D., 2003, Effects of salicylic acid on paraquat tolerance in Arabidopsis thaliana plants, J. Plant Physiol., 46, 31-37   과학기술학회마을   DOI   ScienceOn
13 Reuber, S., Bornman, J. F., Weissenbock, G., 1996, A Flavonoid mutant of barley (Hordeum vulgare L.) exhibits increased sensitivity to UV-B radiation in the primary leaf, Plant Cell Environ., 593-601
14 Kramer G. F., Norman H. A., Krizek D. T., Mirecki R. M., 1991, Influence of UV-B radiation on polyamine , lipid peroxidation and membrane lipids in cucumber, Phytochem., 30, 2101-2108   DOI   ScienceOn
15 Srivastava M. K., Dwivedi U. N., 1998, Salicylic acid modulates glutathione metabolism in pea seedlings, J. Plant Physiol., 153, 409-414   DOI   ScienceOn
16 Janda T., Szalai G., Tari I., Paldi E., 1999, Hydroponic treatment with salicylic acid decreases the effects of chilling injury in maize(Zea mays L.) plants, Planta, 208, 175-180   DOI
17 Kubo A., Aono M., Nakajima N., Saji H., Tanaka. K., Kondo N. 1999, Differential responses in activity of antioxidant enzymes to different environmental stresses in Arabidopsis thaliana, J. Plant Res., 112, 279-290   DOI
18 Alscher R. G., Donahue J. L., Cramer C. L., 1997, Reactive oxygen species and antioxidants : relationships in green cells, Physiol. Plant, 100, 224-233   DOI   ScienceOn
19 Kondo, N., Kawashima, M., 2000, Enhancement of the tolerance to oxidative stress in cucumber (Cucumis sativus L.) seedlings by UV-B irradiation : possible involvement of phenolic compounds and antioxidative enzymes, J. Plant Res., 113, 311-317   DOI
20 Barabas K. N., Szegletes Z., Pestenacz A., Fulop K., Erdel L., 1998, Effect of excess UV-B irradiation on the antioxidant defence mechanisms in wheat(Triticum aestivum L.) seedlings, J. Plant Physiol., 153, 146-153   DOI   ScienceOn
21 Olsson, L. C., Veit, M., Welssenbock, G., Bornman, J. F., 1998, Differential flavonoid response to enhanced UV-B radiation in Brassica napus, Phytochem., 49, 1021-1028   DOI   ScienceOn
22 Surplus S. L., Jordan B. R., Murphy A. M., Carr J. P., Thomas B., Mackerness S. A. H., 1998, UV-B induced responses in Arabidopsis thaliana : role of salicylic acid and reactive oxygen species in the regulation of transcripts encoding photosynthetic and acidic pathogenesis-related proteins, Plant Cell Environ., 21, 685-694   DOI   ScienceOn
23 Zhao S. J., Xu C. C., Zhou Q., Meng Q. W., 1994, Improvements of method for measurement of malondialdehyde in plant tissues, Plant Physiol. Comm., 30, 207-210
24 Beyer W. F., Fridovich I., 1987, Assaying of superoxide dismutase activity : some large sequences of minor changes in conditions, Anal. Biochem., 161, 559-566   DOI   ScienceOn
25 Tanaka K., Kondo N., Sugahara K., 1982, Accumulation of hydrogen peroxide in chloroplasts of $SO_2-fumigated$ spinach leaves, Plant Cell Physiol., 23, 999-1007
26 Karpinski S., Wingsle G., Karpinska B., Hallgren J. E., 2001, Redox sensing of photooxidative stress and acclimatory mechanisms in plants, In : Aro E. M., Andersson B.(eds), Regulation of Photosynthesis, Kluwer Acadmic Publishers, Dordrecht, The Netherlands, pp. 469-486
27 Balakumar T., Gayathri B., Anbudurai P. R., 1997, Oxidative stress injury in tomato plants induced by suppemental UV-B radiation, Biol. Plant, 39, 215-221   DOI
28 Nakano Y., Asada K., 1981, Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts, Plant Cell Environ., 22, 867-880   DOI   ScienceOn
29 Manthe B., Schulz M., Schabl H., 1992, Effects of salicylic acid on growth and stomatal movements of Vicia faba : evidence for salicylic acid metabolization, J. Chem. Ecol., 18, 1525-1539   DOI   ScienceOn
30 Gutierrez-Coronado M. A., Trejo-Lopez C., Larque-Saavedra A., 1998, Effects of salicylic acid on the growth of roots and shoots in soybean, Plant Physiol. Biochem., 36, 563-565   DOI   ScienceOn
31 Jayakumar, M., Amudha P., Kulandaivelu G., 2004, Effect of low doses of UV-A and UV-B radiation on photosynthetic activities in Phaseolus mungo L., J. Plant Biol., 47, 105-110   DOI   ScienceOn