• Parasites, Hosts and Diseases
• /
• 제57권2호
• /
• pp.197-200
• /
• 2019

Cryptosporidium is a common intestinal protozoan that can lead to diarrhea in humans and dogs. The predominant species of infection are C. hominis and C. parvum in humans, and C. canis in dogs. However, C. canis can infect immunocompromised humans. Considering the close contact with humans, dogs have the potential to be reservoirs for human cryptosporidiosis. Breeding kennels are the major supply source of puppies for pet shops. The present study is to determine the molecular prevalence and characteristics of Cryptosporidium spp. found in breeding kennel dogs. A total of 314 fecal samples were collected from young and adult dogs kept in 5 breeding kennels. A polymerase chain reaction targeting the small subunit rRNA gene was employed for the detection of Cryptosporidium spp. To determine the species, the DNA sequences were compared to GenBank data. Overall, 21.0% of the fecal samples were positive for Cryptosporidium spp. infection. Cryptosporidium spp. was detected in all 5 facilities. A sequencing analysis demonstrated that all isolates shared 99-100% similarity with C. canis. The results suggest that Cryptosporidium spp. infection is present at a high-level in breeding kennel dogs. However, because dominant species in this survey was C. canis, the importance of breeding kennel dogs as reservoirs for Cryptosporidium spp. transmission to humans is likely to be low in Japan.

• 한국동물위생학회지
• /
• 제21권4호
• /
• pp.391-400
• /
• 1998

In order to survey the prevalence of intestinal parasites in dogs, 304 fecal samples were taken from dogs in Taejon city, The prevalence and identification of intestinal parasites were determined by the fecal examinations using sheather's floating technique and sedimentation methods and then Cryptosporidium oocysts were identified by kinyoun's modified acid fast stainning method. The results were obtained as follows ; 1. Parasite eggs and oocysts were detected in 105 samples (34.5%) from 304 cases of dog feces. 2. The 11 kinds of eggs and oocysts were isolated from the feces of dog. Those were Ancylostoma caninum (12.1%, 37 dogs), Trichuris vulpis (11.5%, 35 dogs), Toxocara canis (10.2%, 31 dogs), Isospora sp (7.2%, 22 dogs), Cryptosporidium sp (3.6%, 11 dogs), Toxascaris leonine (1.9%, 6 dogs), Strongyloides sp (1.9%, 6 dogs), Taenia sp (0.6%, 2 dogs), Diphylidium caninum (0.3%, 1 dog), Spirometra sp (0.3%, 1 dog) and Clonorchis sinensis (0.3%, 1 dog). 3. It was mixed infection such as single, double, triple and quadruple, 64.8%, 25.7%, 8.6% and 0.9%, respectively. 4. In indiviually-raised 4095, the infectious late of T canis (11.4%), A Caninum(13.2%), Cryptosporidium sp (6.1%), T leontna (2.6%) were higher than those of group raised dogs. But the infectious rate of T vulpis (12.1%) in group raised dogs was higher than that of individually-raised dogs. 5. Adults of Demodex and Sarcoptes which have been found in this survey are excluded in this report.