• Restorative Dentistry and Endodontics
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• v.30 no.2
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• pp.79-85
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• 2005

The purpose of this study was to evaluate the obturation efficiency of a non-standardized gutta-percha cone in curved root canals prepared with 0.06 taper nickel-titanium instruments. Sixty simulated curved root canals in clear resin blocks were prepared with crown-down technique using 0.06 taper rotary $ProTaper^{TM}$and ProFile (Dentsply-Maillefer) until apical canal was size 30. Root canals were randomly divided into 4 groups of 15 blocks and obturated with cold-laterally compacted gutta-percha technique by using either a non-standardized size medium gutta-percha cone or an ISO-standardized size 30 one as a master cone. Gutta-percha area ratio were calculated at apical levels of 1, 3 and 5 mm using AutoCAD 2000 after cross-sectioning, and the data were analyzed with one-way and two-way ANOVAs and Duncan's multiple range test. Non-standardized size medium cone groups showed significantly higher gutta-percha area ratio than standardized cone groups at all apical levels (p < 0.01). Non-standardized cone groups used significantly less accessory cones than standardized cone groups (p < 0.01).

• Restorative Dentistry and Endodontics
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• v.32 no.4
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• pp.385-396
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• 2007

The aims of this study were to compare the shaping effect and safety between single length technique recommended by manufacturer and crown-down technique using Mtwo rotary file and to present a modified method in use of Mtwo file. Sixty simulated root canal resin blocks were used. The canals were divided into three groups according to instrument and the manner of using methods. Each group had 20 specimens. Group MT was instrumented with single length technique of Mtwo, group MC was instrumented with crown-down technique of Mtwo and group PT was instrumented with crown-down technique of ProTaper. All of the rotary files used in this study were operated by an electric motor. The scanned canal images of before and after preparation were superimposed. These superimposed images were evaluated at apical 1 to 8 mm levels Angle changes were calculated. The preparation time, weight loss, instrument failure and binding, canal aberrations, and centering ratio were measured. Statistical analysis of the three experimental groups was performed with ANOVA and Duncan's multiple range tests for post-hoc comparison and Fisher's exact test was done for the frequency comparison. In total preparation time, group MT and group MC were less than group PT. In the aberrations, group MT had more elbows than those of group MC and group PT. The binding of group MC was least and group MT was less than group PT (P < 0.05). Under the condition of this study, crown-down technique using Mtwo rotary file is better and safer method than single length technique recommended by the manufacturer.

• The korean journal of orthodontics
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• v.34 no.1 s.102
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• pp.47-61
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• 2004

Although the purpose of orthodontic treatment is to increase the function and esthetics of the jaws along with increasing stability, there are many side effects during the treatment itself, such as root resorption and alveolar bone resorption. Such resorption of the apical root Is unpredictable, and may even proceed into the dentin layer. Once the process has begun, it is irreversible. By evaluating the effect of many oral habits, especially that of nail biting, in correlation with the root and the periodontal tissues, the appropriate biomechanics for orthodontic treatment can be taken into consideration, along with the possibility of root resorption and alveolar bone loss during orthodontic treatment, and any legal problems that might occur. Among the male and female patients of the ages $10\~15$ without skeletal deformity, 63 were chosen as the experiment group with known nail biting habits at time of examination, and within the same age group without nail biting habits as the control. After the orthodontic treatment, number of the experiment group was 31 and the control group was 22. The periapical radiographies of anterior teeth were taken and the assesment of the root length and alveolar bone level were taken before(T1) and after(T2) the orthodontic treatment. The results from this study were as follows : 1. Before the orthodontic treatment, average crown-to-root ratio of the experimental group showed noticeably high values in 4 maxillary incisors and mandibular right central incisor. 2. Before the orthodontic treatment, comparing the root length, maxillary and mandibular right central incisors and both mandibular incisors had a smaller value in the experimental group. 3. Before the orthodontic treatment, comparing and evaluating the alveolar bone loss measured from the cemento-enamel junction to the alveolar bone crest, some crestal bone of the experiment group showed greater loss than the control. 4. After the orthodontic treatment, there was shortening of the root length and loss of the crestal bone in both groups. 5. After the orthodontic treatment, the changes of C/R ratio and the shortening of root length were significantly high in the experimental group. 6. After the orthodontic treatment, the level of alveolar crestal bone showed greater loss in the experimental group.

• Journal of Dental Rehabilitation and Applied Science
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• v.23 no.4
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• pp.293-302
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• 2007

I. Objectives The purpose of this study was to compare the shaping ability between the single length technique performed with Mtwo instruments (VDW, Munich, Germany) and the crown-down technique using K3 (SybronEndo, West Collins, CA, USA) and RaCe (FKG, La Chaux-de-Fonds, Switzerland) instruments. II. Materials & Methods Forty five curved canals in resin blocks were equally divided in to three groups. Group 1 (Mtwo) was instrumented used the full length of canal according to the manufacturer's instructions. The simulated canals was prepared to an instrument size of 35, 0.04 taper canal terminus. In group 2 (Race) and group 3 (K3) was instrumented in a crown-down manner and prepared to an instrument size of 30, 0.06 taper canal terminus. Pre- and post-instrumentation images were scanned and assessment of canal shape was completed with a computer image analysis program. Material removal was measured at 7 measuring points, beginning 1mm from the end point of preparation. Differenced of centering ratio were statistically analyzed using One-way ANOVA followed by Duncan's test. II. Results & Conclusion There was no significant difference on 1, 2, 3 and 7mm measuring point. At 4 and 5 measuring point, significant difference showed between the Mtow instruments and other two instruments. (p<0.05)

• The Journal of Natural Sciences
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• v.4
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• pp.103-142
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• 1991

These studies were carried out to select somatic hybrid using selectable marker genes of Nicotiana glauca transformed by NPTII gene and Solanum tuberosum transformed by T- DNA, and to study characteristics of transformant. The results are summarized as follows. 1. Crown gall tumors and hairy roots were formed on potato tuber disc infected by A. tumefaciens Ach5 and A. rhizogenes ATCC15834. These tumors and roots could be grown on the phytohormone free media. 2. Callus formation from hairy root was prompted on the medium containing 2, 4 D 2mg/I with casein hydrolysate lg/l. 3. The survival ratio of crown gall tumor callus derived from potato increased on the medium containing the activated charcoal 0. 5-2. 0mg/I because of the preventions on the other hand, hairy roots were necrosis on the same medium. 4. Callus derived from hairy root were excellently grown for a short time by suspension culture on liquid medium containing 2, 4-D 2mg/I and casein hydrolysate lg/l. 5. The binary vector pGA643 was mobilized from E. coli MC1000 into wild type Agrobacteriurn tumefaciens Ach5, A. tumefaciens $A_4T$ and disarmed A. tuniefaciens LBA4404 using a triparental mating method with E. ccli HB1O1/pRK2013. Transconjugants were obtained on the minimal media containing tetracycline and kanamycin. pGA643 vectors were confirmed by electrophoresis on 0.7% agarose gel. 6. Kanamycin resistant calli were selected on the media supplemented with 2, 4-D 0.5mg/1 and kanamycin $100\mug$/ml after co- cultivating with tobacco stem explants and A. tumefaciens LBA4404/pGA643, and selected calli propagated on the same medium. 7. The multiple shoots were regenerated from kanamycin resistant calli on the MS medium containing BA 2mg/l. 8. Leaf segments of transformed shoot were able to grow vigorusly on the medium supplemented with high concentration of kanamycin $1000\mug$/ml. 9. Kanamycin resistant shoots were rooting and elongated on medium containing kanamycin $100\mug$/ml, but normal shoot were not. 10. For the production of protoplast from potato calli transformed by T-DNA and mesophyll tissue transformed by NPTII gene, the former was isolated in the enzyme mixture of 2.0% celluase Onozuka R-10, 1.0% dricelase, 1.0% macerozyme. and 0.5M mannitol, the latter was isolated in the enzyme mixture 1.0% Celluase Onozuka R-10, 0.3% macerozyme, and 0.7M mannitol. 11. The optimal concentrationn of mannitol in the enzyme mixture for high protoplast yield was 0.8M at both transformed tobacco mesophyll and potato callus. The viabilities of protoplast were shown above 90%, respectively. 12. Both tobacco mesophyll and potato callus protoplasts were fused by using PEG solution. Cell walls were regenerated on hormone free media supplemented with kanamycin after 5 days, and colonies were observed after 4 weeks culture.

• Journal of the Korean Institute of Landscape Architecture
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• v.29 no.1
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• pp.152-160
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• 2001

This study is conducted to evaluate the effects of containerized landscape tree production methods on post-transplant stress. Two types of container such as plastic pot(pot), fabric growing bag(bag) were adopted to restrict tree roots. Each types of containers was divided into seven sub-types. One traditional production method was included as comparison. Two landscape woody plants species (Magnolia denudata, Albizzia julibrissin) were implanted in the 7 sub-types of container. After one or two growing season in the container, each types of container trees was transplanted. Half of the trees were transplanted in the mild spring season, and another half of trees were transplanted in the improper summer season. The data were collected on the crown wilting ratio and trunk die-back ratio. The result of the analysis based on these data were as follows; 1) The container production methods were lower than the traditional production methods by 3 times in the average wilting ratio of summer season's transplanting point. 2) Post-transplant stress was more successfully mitigated, in case the "pot" type as was the "bag" types of container. 3) The effective and economic way of mitigating post-transplant stress by container production methods was selecting container plants of vigorous and deep root systems. 4) The "pot" type of container was to restrict tree roots more successfully, But, winter chilling and low temperature attacked the "pot" type tree's twigs and suckers. These results indicated that "pot" grown container plants should managed carefully during the winter after transplanting. Based upon the results of this study, a subsequent research on the development of container material, growth type of the container trees, and other maintaining method will be required.es, and other maintaining method will be required.

• Restorative Dentistry and Endodontics
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• v.6 no.1
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• pp.123-132
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• 1980

The author conducted a dental survey of a college girl students and compared with that of E college students 10 years ago which based on the same criteria. The items of this survey included the prevalence of dental caries, DMFT and the kinds of restored material. All college students 1,414 were examined in May 1978, with the recommended criteria and method of W.H.O.. Among them, the data of 1,393 were analyzed for this study. The following results were obtained: 1. The average number of present teeth were 28.97${\pm}$1.64. 2. The prevalence of dental caries was 84.92% and D.M.F. index was 15.51. Average D.M.F. teeth were 4.49${\pm}$3.69. 3. The average number of decayed (D) teeth were 2.09${\pm}$2.16. 4.30% of the present decayed teeth was secondary decay, and 2.44% of the decayed teeth was indicated for extraction. 4. The average number of missing(M) teeth were 0.32${\pm}$0.79. It included 8.56% of root fragements. 5. Average number of filled(F) teeth were 2.09${\pm}$3.17. F-ratio of this group was 46.45%. The analysis revealed 50.88% of amalgam alloy, 31.41% of gold inlays, and 1.20%, the least among the filling materials, silicate cement or composite resin. 6. While the prosthodontic treatment, such as crown and bridge has reduced than that of 10 years ago, the fillings and inlays as the conservative means has increased.

• Korean Journal of Ecology and Environment
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• v.47 no.1
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• pp.32-40
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• 2014

This study was conducted to evaluate the growth response of economical six leafy vegetables that are crown daisy, pak-choi and four kinds of lettuce (Red leaf lettuce, Green leaf lettuce, Head lettuce, Romaine lettuce) by light treatment of LED in plant factory. The light treatments were composed of red, blue, red+farred, red+blue, red+blue+white LEDs, irradiation time ratio of the red and blue LED per minute (1 : 1, 2 : 1, 5 : 1, 10 : 1), and duty ratio of mixed light (100%, 99%, 97%). The following results were obtained in different LED light sources treatments: Shoot biomass and S/R ratio of romaine lettuce were the highest under mixed red+blue LEDs. S/R ratio of head lettuce was higher under mixed red+blue+white LEDs than red+blue LEDs. The others showed no difference in LED light treatment. Shoot biomass, total biomass and S/R ratio of green lettuce, head lettuce and pak-choi were highest in the higher red ratio (5 : 1) on irradiation time of red : blue LED ratios. By the different duty ratio (red+blue and red+blue+white LEDs), Under the mixed light of red+blue, shoot and root biomass of crown daisy and romaine lettuce were high in duty ratio of 100% and 99%, and S/R ratio was highest in all the 6 kinds in duty ratio of 97%. All the 6 kinds showed a fine growth state in low duty ratio (97%). Green lettuce, romaine lettuce and pak-choi showed relatively high shoot biomass and total biomass in low duty ratio of 97% under the mixed light of red+blue+white. S/R ratio of romaine lettuce and head lettuce were highest in the duty ratio of 97% with red+blue+white LEDs. Thus, we can cultivate stably without reference to external factors, if we use appropriate light sources and light quality in closed-type plant factory.

• Journal of Korean Society of Forest Science
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• v.88 no.4
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• pp.454-461
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• 1999

The dynamics of root system in the fairy rings of Tricholoma matsutake was investigated in four zones divided into 'zone of decayed mycorrhizae', 'zone of mycorrhizae for fruiting', 'zone of physiologically active mycorrhizae' and 'zone of roots free from mycorrhizal infection'. The roots of Pinus densiflora in fairy rings, which occupied 70% of upper crown, comprised about 60% and 87% of total roots and alive roots, respectively. The ratio of fine-roots of P. densiflora over total roots was relatively higher than other species since that of P. densiflora was about 45% while that of the other species was about 13% on research of fine-roots. Especially, the roots of pine comprised about 70% of total root in the zone of mycorrhizae for fruiting and the zone of physiologically active mycorrhizae, which indicated that the pine roots were closely related to the fairy rings of T. matsutake. The fine roots of P. densiflora in the zone of physiologically active mycorrhizae was about 60.7%(1,087mg/100g soil) which was about twice compared to that of other zones. It allowed us to suppose that the fine roots of P. densiflora can make active growth in the zone of physiologically active mycorrhizae, and the growth was promoted by the fairy ring formation of T. matsutake. In addition, we found 3~5 times higher amount of fine roots than that of medium roots of P. densiflora in this zone, which indicated that the fairy rings of T. matsutake locate in the region of active growth of P. densiflora' roots.

• Journal of Bio-Environment Control
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• v.20 no.4
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• pp.283-289
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• 2011

This study was conducted to examine the effects of defoliation treatment on the growth and yield of strawberry ($Fragaria{\times}ananassa$ cv. Maehyang and Seolhyang) during nursery period. Leaves of strawberry plantlet had been removed except two, three and four fully expanded leaves until planting date. As the intensity of defoliation was strong, the petiole length was reduced and overgrowth of strawberry plantlet was suppressed. Outer diameter of crown in defoliation treatments significantly decreased but inner diameter of crown was not significant. Number of primary roots of the 3 leaves or 4 leaves defoliation treatment generally tended to increase, but there was not significantly different among treatments. Fresh weight and leaf area in the defoliation treatments significantly decreased and the root weight were higher in partial 3 leaves or 4 leaves defoliation treatment but was not significantly different among treatments. Because T/R ratio decreased significantly as growth inhibition of above-ground part compared to underground part, it is considered easy to take rooting after plantlet plating. As the intensity of defoliation was strong, chlorophyll contents tended to decrease significantly. Reduction of the endogenous nitrogen by defoliation effectively led to promote floral differentiation at low temperature and short day condition. This promoted timing of budding and flowering and also induced uniform flowering after plantlet planting. Marketable fruit yield of 3 leaves defoliation treatment tended to be higher than the control.