• Title/Summary/Keyword: Crossing Culture

Search Result 97, Processing Time 0.03 seconds

In Vitro Culture of Immature Embryo Obtained by Crossing between Tetraploid Grape 'Fujiminori' and Triploid 'Summer Black' (포도 4배체 '후지미노리'와 3배체 '썸머블랙'의 교배로 얻은 미숙배의 기내배양)

  • Koh, Jae Chul;Oh, Ju Eun
    • Horticultural Science & Technology
    • /
    • v.31 no.3
    • /
    • pp.352-358
    • /
    • 2013
  • For the germination and differentiation of immature embryos obtained by artificial crossing between tetraploid grape 'Fujiminori' (Vitis vinifera ${\times}$ V. labruscana) and triploid 'Summer Black' (V. labruscana ${\times}$ V. vinifera), were incubated in vitro using MS medium supplemented with $GA_3$ or coconut water (CW) at various concentrations. The percentage of embryo formation of 'Fujiminori' ${\times}$ 'Summer Black' was 64.3%. Embryo germination percentage was higher than 95% in all the $GA_3$ treatments at the concentrations of 0.01, 0.05, 0.25, and $1.25mg{\cdot}L^{-1}$. However, only 15.8-31.6% of the germinated embryos successfully developed into normal plantlets. At higher concentration of $GA_3$, the plantlets developed infirm hypocotyls with over elongated and less enlarged structure. Among the treatments of CW at the concentrations of 5, 10, 15, and 20% (v/v), 10% and 15% were more effective and plantlet achievement percentage were 68.4 and 66.7%, respectively. The addition of 10% CW was most effective to obtain plantlets with optimal shoot length, node and root numbers. 15% CW was suitable to obtain plantlets with longer roots. Accordingly, the embryo culture using the MS medium supplemented with 10-15% CW was observed to be more efficient for germinating and growing the immature embryos produced from artificial crossing between tetraploid grape 'Fujiminori' and triploid 'Summer Black'.

Rethinking Fashion or the Anthropology of Fashion through the Anthropology of Aft: A Case of the Vivienne Westwood Exhibition at the V&A

  • Lee, Jeong-Taek
    • Proceedings of the Korea Fashion and Costume Design Association Conference
    • /
    • 2004.06a
    • /
    • pp.13-20
    • /
    • 2004
  • This study aims to rethink fashion or, namely, the anthropology of fashion through examining issues generated by the relationship between art and the anthropology of art. Since their crossing paths, sound discussions have emerged between the anthropology of art and the art world, such as subjects about 'artworks and artefacts', 'Western and non-Western discourse' and 'art and agency'(Gell 1992; 1993; 1996; 1998).(omitted)

  • PDF

A Study of Modern Art and Fashion Trend Image -Based on after 1995 Fashion Trend- (현대예술과 패션의 트랜드 이미지에 관한 연구 -95년 이후 Fashion Trend를 중심으로-)

  • 이은영
    • The Research Journal of the Costume Culture
    • /
    • v.6 no.3
    • /
    • pp.88-99
    • /
    • 1998
  • Image of Modern Art & Fashion are created on the whole in daily life. Image itself is not simple. There is no more distinction between content & form through mutual crossing for intellectual thinking. Specially, image of reproduction circumstance too many mixtual and compromise (collage) are continued to occur. They could pursuit to change their image through the more increase of impulse, exception and popular experience. In this thesis, I studied about image of Modern fashion & Art. In the modern art, visual image showed as anti-beauty and combination of cultural system. It has shown in the theory of Fred Davis and through fashion brand advertising image as follows; Youth vs maturity (ageless image), male s female and androgynous vs unisexuality (genderless image), colligation vs monopoly (street mode image). As a results, the modern trend image are showing the value of the ambivalence. It can be said that he anti-beauty which are occasionally showed in ambivalence has shown a tendency toward another tradition (feminity) through giving an impulse and paradox.

  • PDF

Imperialism, Nationalism, and Humanism: A Comparative Study of The Red Queen and Song of Ariran (제국주의, 민족주의, 그리고 휴머니즘 -『적색의 왕비』와 『아리랑 노래』의 비교 연구)

  • Park, Eun Kyung
    • English & American cultural studies
    • /
    • v.9 no.1
    • /
    • pp.239-272
    • /
    • 2009
  • Our investigation of the intricate relationship among nationalism, humanism, and imperialism begins from reading Song of Ariran, the auto/biography of Kim San recorded by Nym Wales, together with Margaret Drabble's fictional adaptation of Lady Hong's autobiography, The Memoirs of Lady $Hyegy{\breve{o}}ng$, in her novel The Red Queen, in which the story of Barbara Halliwell, a modern female envoy of Lady Hong, is interweaved with Lady Hong's narrative. In spite of their being seemingly disparate texts, Song of Ariran and The Red Queen are comparable: they are written by Western female writers who deal with Koreans, along with the Korean history and culture. Accordingly, both works cut across the boundary of fiction and fact, imagination and history, and the East and the West. In the age of globalization, Western women writing (about) Korea and Koreans traversing the historical and cultural limits inevitably engage us in post-colonial discussions. Despite the temporal differences--If Song of Ariran handles with the historical turmoils of the 1930s Asia, mostly surrounding Kim San's activities as a nationalist, The Red Queen is written by a twenty-first century British woman writer whose international interest grapples with the eighteenth-century Korean Crown Princess' spirit in order to reinscribe a story of Korean woman's within the contemporary culture--, both works appeal to the humanistic perspective, advocating the universal human beings' values transcending the historical and national limitations. While this sort of humanistic approach can provide sympathy transcending time and space, this 'idealistic' process can be problematic because the Western writers's appropriation of Korean culture and its history can easily reduce its particularities to comprehensive generalization, without giving proper names to the Korean history and culture. Nonetheless, the Western female writers' attempt to find a place of 'contact' is valuable since it opens a possibility of having meaningful communications between minor culture and dominating culture. Yet, these female writers do not seem to absolutely cross the border of race, gender, and culture, which leaves us to realize how difficult it is to reach a genuine understanding with what is different from mine even in these 'universal' narratives.

Breeding of New Varieties by Ovule Culture of Intergeneric Hybrid in the Aurantioideae (속간교잡 후 배주배양에 의한 감귤류 신품종 육성)

  • 이만상
    • Korean Journal of Plant Tissue Culture
    • /
    • v.22 no.5
    • /
    • pp.261-266
    • /
    • 1995
  • This study was carried out to develop new varieties which are dwarf and tolerant to winter cold in the Aurantioideae by intergeneric crossing. to do that, the reciprocal crosses of Hwanggeumyooza and trifoliate orange, yooza and trifoliate orange were done and in vitro immature ovule culture of their hybrid was carried out .The callus formation from immature ovule was good in order of Hwanggeumyooza, Hwanggeumyooza $\times$ tifoliate orange, yooza, and trifoliate orange and best at 1 to 3 mg/L NAA+0.5mg/L zeatin on MT medium. In vitro germination percentage of 20week old hybrid of Hwanggeumyooza $\times$ tifoliate orange and trifoliate orange $\times$ Hwanggeumyooza were 41.3% and 37.7, respectively. The phenotype of hybrid (95%) of Hwanggeumyooza $\times$ trifoliate orange and that (100%) of trifoliate orange $\times$ Hwanggeumyooza were similar to that of trifoliate orange. After Hwanggeumyooza was pollinated by pollens of trifoliate orange, the pollen tubes grew on stigma after 3h of pollination and entered into micropyle after about 24~28 h. One gamete in pollen was fused with polar nuclei after 2 days and other one fused with egg nucleus at 3days after pollination. The fruit set percentage by intergeneric crossing was 14.0% in Hwanggeumyooza $\times$ trtfoliate orange and 17.5% in trifoliate orange $\times$ Hwanggeumyooza. The fruit set percentages of Hwanggeumyooza. and trifoliate orange were 34.2% and 39.5% by artificial self-fertilization, 34.2% and 39.5% by artificial cross fertilization, 3.1% and 1.4% by parthenocarpy and 13.0% and 3.0% by natural fertilization, respectively. The somatic and gametic chromosome numbers of Hwanggeumyooza, yooza, and trifoliate orange were 2n=18 and n=9.

  • PDF

Plant Regeneration via in Vitro Culture of Ovule Obtain by Intergeneric Crossing Between Citrus junos Sieb. et Tanaka and Poncirus trifoliata Raf. (유자와 탱자의 속간교잡후 배주배양에 의한 식물체 유기)

  • 이만상;남궁승박
    • Korean Journal of Plant Tissue Culture
    • /
    • v.22 no.6
    • /
    • pp.317-322
    • /
    • 1995
  • As a basic research for breeding new varieties, reciprocal -intergeneric crosses between Citrus junos and P.trifoliata were made. F$_1$ hybrid production using in vitro ovule culture, gametogenesis, and fertilization phenomena were investigated. Frequency of fruit set resulting from crossing of Citrus junos and Poncirus Trifoliata was 16.6% while that of Poncirus Trifoliata and Citrus junos was 11.7%. Callus formation occurred well when ovules at the 6th week after pollination were cultured on MT (Murashige and Tucker) medium supplemented with zeatin 0.5 mg/L and NAA 1.0 or 3.0 mg/L. Immature ovules developed into mature embryos of the MT medium supplemented with 2,4-D 0.1 or 3.0 mg/L. Immature ovules developed into mature embryos of the MT medium supplemented with 2,4 D 0.1 or 0.5 mg/L. The invitro germination rates of 20-week-old ovules set C. junos $\times$ P. Trifoliata and P. Trifoliata $\times$ C. junos were 54.5% and 48.6%, respectively. The emergence ratios of trifoliate hybrids obtained by C. junos $\times$ P. Trifoliata and P. Trifoliata $\times$ C. junos were 56.7% and 100%, respectively. The chromosome number of C. junos and P. Trifoliata was n = 9 or 2n = 18, and the sizes of their pollen grain were 33.75 $\mu$ and 25.0 $\mu$. The length and width of embryo sac in C. junos and P. Trifoliata were 69.38~79.23 $\mu$ and 27.50~38.56 $\mu$, and those of egg cells were 17.50~41.50 $\mu$ and 6.25~8.12$\mu$. Fertilization of C. junos and P. trifoliata terminated 72 h after pollination.

  • PDF

Physiological Study on the Hybrid by Interspecific Crossing between Porphyra pseudolinearis and P. dentata (Bangiales, Rhodophyta), Two Dioecious Species in Culture (잇바디돌김(Porphyra dentata Kjellman)과 긴잎돌김(Porphyra pseudolinearis Ueda)의 종간 교잡종의 생리특성)

  • 김남길
    • Journal of Aquaculture
    • /
    • v.13 no.4
    • /
    • pp.353-357
    • /
    • 2000
  • Porphyra pseudolinearis and P. dentata from Korea were crossed and the hybrid was cultured at different temperatures (5, 10, 15, 20 or $25^{\circ}C$), photon flux densities (10, 20, 40 or 80${\mu}$mol m$^{-2}$s$^{-1}$) under photoperiods (14L:10D and 10L:14D). In the hybrids, the conchocelis grew faster at 2$0^{\circ}C$ and 40$\mu$mol m$^{-2}$ s$^{-1}$ at $25^{\circ}C$ only, and were abundant, when cultured under 10L:14D. Foliose thalli of the hybrid grew rapidly at conditions of 10-2$0^{\circ}C$, 10L:14D and 15-2$0^{\circ}C$, 14L:10D but slowly at 5 and 2$0^{\circ}C$. No archeospores were observed any tested culture condition. Spermatangial and zygotosporangial sori were formed at the marginal portion o mature thallus. Zygotospores from the hybrid were released at 10-2$0^{\circ}C$ under both photoperiods, and gave rise to form conchocelis filament. Monoecious thalli were observed at 1$0^{\circ}C$ under 14L:10D. Neither monospores nor protothalli were produced from the conchocelis in culture.

  • PDF

Food-Networks and Border-Crossing of Transnational Marriage Migrant Households (초국적 결혼이주가정의 음식: 네트워크와 경계 넘기)

  • Choi, Byung-Doo
    • Journal of the Korean association of regional geographers
    • /
    • v.23 no.1
    • /
    • pp.1-22
    • /
    • 2017
  • This paper is to consider conceptually a formation of food-networks and border-crossing of transnational marriage migrant households on the basis of actor-network theory, and to analyze empirical data on the issues collected by interview with marriage migrant women living around Daegu, S.Korea. Some research results can be argued as follows: First, food can be seen, not as a single material object, but as a multiple and hybrid network of human and nonhuman (material and institutional) actors, in which activities of food cooking and eating are regulated by and (re)construct social relations and placeness of households. Secondly, food-networks in marriage migrant households implement relationships of micro-power (and attachment) in the process of its (re)formation, and hence the food-network, it can be argued, is a field of power in which conflicts and compromising around food cooking and eating are intersecting each others. Thirdly, food-networks in marriage migrant households in both their origin country and in the Korean home are not only affected by macro natural and social environments but also by micro placeness of the households, both of which constitute the food-networks and operate in relations with other actors in the netwroks. Finally, food-networks in marriage migrant households reflect multiple and multi-scalar spatial mobility and placeness of transnational food culture, through which they express topologically 'fluid space' and 'absent presence', in which marriage migrant women can (or cannot) conduct social and cultural border-crossing.

  • PDF

Characterization of a new white variety "Baengno" developed by crossing with selected lines adaptable to elevated-temperature in Flammulina velutipes (고온적응성 선발계통을 이용한 팽이버섯 신품종 '백로'의 특성)

  • Kong, Won-Sik;Seo, Kyoung-In;Park, Soon-Young;Jang, Kab-Yeul;Yoo, Young-Bok;Jhune, Chang-Sung;Kim, Kwang-Ho
    • Journal of Mushroom
    • /
    • v.6 no.3_4
    • /
    • pp.121-125
    • /
    • 2008
  • A first, we made white strain 'S line' adaptable to elevated temperature after collected brown strains were characterized with culture characterization on temperature and their monokaryons isolated at the high temperature were crossed with white strain. These S line monokaryons were crossed again with other monokaryons derived from a commercial white. As a result of successive crossing, finally a new variety 'Baengno' was developed. The optimum temperature of mycelial growth was $25^{\circ}C$ but it needed to adjust to $18{\sim}20^{\circ}C$ when incubated at the bottle cultivation. It brought incubation period shortening as 21 days saved 2~3 days when compared with other white varieties. The optimum temperatures of fruiting body initiation and development were almost same to others like as $14^{\circ}C$ and $7^{\circ}C$, respectively. Fruiting body of 'Baengno' was pure white even developed from crossing with brown strains. 'Baengno' was a good variety with high quality and high productivity characterized as quite even budding habit, long stipes and hemi-spherical pilei. This variety needed more air ventilation and had to be adjusted its cultivation environment for the good cultivation.

  • PDF

Analysis of flavonoids in double haploid population derived from microspore culture of F1 hybrid of Brassica rapa (배추 종간 잡종의 소포자배양에 의한 Double haploid 집단의 플라보노이드 함량 분석)

  • Seo, Mi-Suk;Won, So Youn;Kang, Sang-Ho;Kim, Jung Sun
    • Journal of Plant Biotechnology
    • /
    • v.44 no.1
    • /
    • pp.35-41
    • /
    • 2017
  • One of the most important species, Brassica rapa encompasses a variety of commercial vegetables, such as the Chinese cabbage, pak choi and oilseed crops. The LP08 of yellow sarson (Brassica rapa ssp, tricolaris) have a distinct morphology, with yellow seed color and a unique tetralocular ovary. LP21 of pak choi (Brassica rapa ssp, chinensis) have a dark brown seed color and bilocular ovary. In this study, we generated double haploid plants by crossing the LP08 (maternal variety) and LP21 (paternal variety), using microspore culture. A total of 66 accessions with various morphological characteristics were used for content analysis of flavonoids. The three flavonoids, quercetin, naringenin and kaempferol, showed differing contents in the two crossing parents. The Chinese cabbage type 'Chiifu' was used as the control. The highest accumulation of total flavonoids was observed in LP08. The lowest mean total flavonoids were found in 'Chiifu'. Among the 66 DH accessions, the quercetin contents of 18 accessions showed higher content than LP08. Kaempferol content was also high, and was found to be 79.7% of the total flavonoid content. Naringenin content was low at 2.8%, and was not detected in 22 accessions. Interestingly, the quercetin content positively correlated with the kaempferol content. These results can be used to identify genetic locus and genes related to useful traits. Phenotypic analysis of 66 DH accessions can further be used for natural selection of good breeding materials in B. rapa.