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Solid-state NMR Study on Membrane Protein Structure in Biological Condition

  • Kang, Su-Jin;Lee, Bong-Jin
    • Journal of the Korean Magnetic Resonance Society
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    • v.16 no.2
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    • pp.103-110
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    • 2012
  • Membrane proteins play a essential role in the biological systems and it is not easy to handle a membrane protein for its structural study. Solid-state NMR (ssNMR) can be a good tool to investigate the structures and dynamics of membrane proteins. In ssNMR, Magic Angle Spinning (MAS) and Cross Polarization (CP) can be utilized to reduce the line-broadening, leading to high resolution and sensitivity in the spectrum. ssNMR, if combined with other spectroscopic methods, can provide us a enough knowledge on structures and dynamics of membrane proteins in biological condition.

Analysis of Russian Culture Education According to the Curriculum Changes (교육과정 변화에 따른 러시아어 문화 교육 내용 분석)

  • Eo, Keon Joo
    • Cross-Cultural Studies
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    • v.29
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    • pp.479-501
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    • 2012
  • In this paper, I analyzed the russian cultural content of the russian textbook according to curriculum changes. The aim of this study is to analyze the content of the russian textbooks on russian culture. Our education of russian language begins in high school as a second language. And russian education in high school entirely depend on the textbook. In these circumstances, Russian textbooks play a very important role in the Russian language learning. For a practical and efficient language learning, acquisition of cultural knowledge is very important. Because cultural content can be learning motivational factors. But the contents of a textbook is not satisfactory enough to teach russian culture. More efficient textbook must be developed to advance student's linguistic ability.

'Behind-the-Border' Regulatory Policies and Trade Agreements

  • Hoekman, Bernard
    • East Asian Economic Review
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    • v.22 no.3
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    • pp.243-273
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    • 2018
  • A consequence of global trade liberalization is that domestic regulatory policies have become a focal point for efforts to reduce the costs of engaging in cross-border production and exchange. This article discusses the general challenges of reducing trade frictions created by regulatory differences, focusing specifically on the role trade agreements might play in addressing regulatory spillovers. A case is made for a greater focus on plurilateral cooperation under the umbrella of the WTO.

Structure and Properties of Segmented Block Copolyetheresters Based on PBT and PTMGT. 2. Mechanical and Dynamic Mechanical Properties

  • Jeon, Byoung-Yeol;Baik, Doo-Hyun
    • Proceedings of the Korean Fiber Society Conference
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    • 1998.10a
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    • pp.33-36
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    • 1998
  • Segmented block copolyetheresters defined as copolymers having sequences of alternating polyester hard blocks and polyether soft blocks create labile physical cross-links upon crystallization of hard polyester blocks Since the nature of the physical interlocking is a crystallite formed exclusively from the crystallizable hard segment, the hard segment content (HSC) and hard segment length (HSL) will play an important role in determining the properties such as mechanical property and dynamic mechanical property. (omitted)

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The Influence of Collision Energy on the Reaction H+HS→H2+S

  • Liu, Yanlei;Zhai, Hongsheng;Zhu, Zunlue;Liu, Yufang
    • Bulletin of the Korean Chemical Society
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    • v.34 no.11
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    • pp.3350-3356
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    • 2013
  • Quasi-classical trajectory calculations have been carried out for the reaction H+HS by using the newest triplet 3A" potential energy surface (PES). The effects of the collision energy and reagent initial rotational excitation are studied. The cross sections and thermal rate constants for the title reaction are calculated. The results indicate that the integral cross sections (ICSs) are sensitive to the collision energy and almost independent to the initial rotational states. The ro-vibrational distributions for the product $H_2$ at different collision energies are presented. The investigations on the vector correlations are also performed. It is found that the collision energies play a postive role on the forward scatter of the product molecules. There is a negative influence on both the alignment and orientation of the product angular momentum for low collision energy at low energy region. Whereas the influence of collision energy is not obvious at high energy region.

Effect of Brush Treatment and Brush Contact Sequence on Cross Contaminated Defects during CMP in-situ Cleaning

  • Kim, Hong Jin
    • Tribology and Lubricants
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    • v.31 no.6
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    • pp.239-244
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    • 2015
  • Chemical mechanical polishing (CMP) is one of the most important processes for enabling sub-14 nm semiconductor manufacturing. Moreover, post-CMP defect control is a key process parameter for the purpose of yield enhancement and device reliability. Due to the complexity of device with sub-14 nm node structure, CMP-induced defects need to be fixed in the CMP in-situ cleaning module instead of during post ex-situ wet cleaning. Therefore, post-CMP in-situ cleaning optimization and cleaning efficiency improvement play a pivotal role in post-CMP defect control. CMP in-situ cleaning module normally consists of megasonic and brush scrubber processes. And there has been an increasing effort for the optimization of cleaning chemistry and brush scrubber cleaning in the CMP cleaning module. Although there have been many studies conducted on improving particle removal efficiency by brush cleaning, these studies do not consider the effects of brush contamination. Depending on the process condition and brush condition, brush cross contamination effects significantly influence post-CMP cleaning defects. This study investigates brush cross contamination effects in the CMP in-situ cleaning module by conducting experiments using 300mm tetraethyl orthosilicate (TEOS) blanket wafers. This study also explores brush pre-treatment in the CMP tool and proposes recipe effects, and critical process parameters for optimized CMP in-situ cleaning process through experimental results.

The onset of extreme fire behaviour in a mine drift

  • Hansen, Rickard
    • Geosystem Engineering
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    • v.21 no.5
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    • pp.282-290
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    • 2018
  • The onset of extreme fire behaviour in a mine drift with longitudinal ventilation was analysed. A fire in a mine drift with continuous fuel load, involving several separate fires may lead to flames tilted horizontally and filling up the entire cross section. This will lead to earlier ignition, higher fire growth rate, higher fire spread rate and a severe fire behaviour. The focus has been on what changes take place at the onset and signs of the impending phenomenon. It was found that the fire gas temperature at the ceiling level provided a poor indicator. At the downstream far-field region of the fire, the sudden temperature increase at the lowest levels of the cross section and the sudden increase in flow velocities would provide signs of extreme fire behaviour. The corresponding full-scale heat release rates of the experiments at the onset of extreme fire behaviour were found to be very high for mining applications but not necessarily for tunnel fires. The heat release rate threshold for a mine drift with smaller cross-sectional dimensions would decrease considerably, increasing the likelihood of occurrence. The distance between the fuel items will play an important role during the initiation of horizontal flames.

Cross-reactivity of Human Polyclonal Anti-GLUT1 Antisera with the Endogenous Insect Cell Glucose Transporters and the Baculovirus-expressed GLUT1

  • Lee, Chong-Kee
    • Biomedical Science Letters
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    • v.7 no.4
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    • pp.161-166
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    • 2001
  • Most mammalian cells take up glucose by passive transport proteins in the plasma membranes. The best known of these proteins is the human erythrocyte glucose transporter, GLUT1. High levels of heterologous expression far the transporter are necessary for the investigation of its three-dimensional structure by crystallization. To achieve this, the baculovirus expression system has become popular choice. However, Spodoptera frugiperda Clone 9 (Sf9) cells, which are commonly employed as the host permissive cell line to support baculovirus replication and protein synthesis, grow well on TC-100 medium that contains 0.1% D-glucose as the major carbon source, suggesting the presence of endogenous glucose transporters. Furthermore, very little is known of the endogenous transporters properties of Sf9 cells. Therefore, human GLUT1 antibodies would play an important role for characterization of the GLUT1 expressed in insect cell. However, the successful use of such antibodies for characterization of GLUT1 expression m insect cells relies upon their specificity for the human protein and lack of cross-reaction with endogenous transporters. It is therefore important to determine the potential cross-reactivity of the antibodies with the endogenous insect cell glucose transporters. In the present study, the potential cross-reactivity of the human GLUT1 antibodies with the endogenous insect cell glucose transporters was examined by Western blotting. Neither the antibodies against intact GLUT1 nor those against the C-terminus labelled any band migrating in the region expected fur a protein of M$_r$ comparable to GLUT1, whereas these antibodies specifically recognized the human GLUT1. Specificity of the human GLUT1 antibodies tested was also shown by cross-reaction with the GLUT1 expressed in insect cells. In addition, the insect cell glucose transporter was found to have very low affinity for cytochalasin B, a potent inhibitor of human erythrocyte glucose transporter.

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Effects of olfactory self- and cross-adaptation on perceiving odor in a moth

  • Qian, Kai;Chen, Haibin;Wan, Xinlong
    • International Journal of Industrial Entomology and Biomaterials
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    • v.32 no.2
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    • pp.60-68
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    • 2016
  • Pheromone orientation in moths is an exemplar of olfactory sensitivity. To avoid cross mating, the responses of males to pheromone blends must be high specificity and temporal resolution. We tested the effects of olfactory self- and cross-adaptation of pheromone compounds and mixtures in Spodoptera litura moths by electroantennogram (EAG) recordings. The challenge of S. litura antennae to a pulse train of its own pheromone blends of Z9,E11-14:OAc and Z9,E12-14:OAc with 200 ms on/off and 1 s on/off indicated that the repetitive stimulation by 200 ms on/off with high dosages resulted in greater adaptation than that by 1 s on/off with low dosages and the adaptation index of Z9,E11-14:OAc in all treatments is significantly larger than that of Z9,E12-14:OAc, suggesting that high dosages with more frequent stimulation prefer to induce sensory adaptations and a different odor coding exist between the two components in the antennal periphery in this moth. The cross-adaptation EAG test among the two pheromone compounds and Z7-12:OAc and Z9-14:OH from congeneric species of S. litura showed that each of these compounds adapted the antenna more to that specific compound. The significantly higher adaptation to Z7-12:OAc and Z9-14:OH than to the pheromone components of S. litura induced by themselves suggested that both of them are coded by specific odor receptor neurons which are different from those tuned to the pheromone components of S. litura . Thus, we proposed that Z7-12:OAc and Z9-14:OH may play an important role in avoidance of heterospecific mating between S. litura and its sympatric moth species.

Correctum : Effects of olfactory self- and cross-adaptation on perceiving odor in a moth

  • Qian, Kai;Chen, Haibin;Wan, Xinlong
    • International Journal of Industrial Entomology and Biomaterials
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    • v.33 no.2
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    • pp.149-149
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    • 2016
  • Pheromone orientation in moths is an exemplar of olfactory sensitivity. To avoid cross mating, the responses of males to pheromone blends must be high specificity and temporal resolution. We tested the effects of olfactory self-and cross-adaptation of pheromone compounds and mixtures in Spodoptera litura moths by electroantennogram (EAG) recordings. The challenge of S. litura antennae to a pulse train of its own pheromone blends of Z9,E11-14:OAc and Z9,E12-14:OAc with 200 ms on/off and 1 s on/off indicated that the repetitive stimulation by 200 ms on/off with high dosages resulted in greater adaptation than that by 1 s on/off with low dosages and the adaptation index of Z9,E11-14:OAc in all treatments is significantly larger than that of Z9,E12-14:OAc, suggesting that high dosages with more frequent stimulation prefer to induce sensory adaptations and a different odor coding exist between the two components in the antennal periphery in this moth. The cross-adaptation EAG test among the two pheromone compounds and Z7-12:OAc and Z9-14:OH from congeneric species of S. litura showed that each of these compounds adapted the antenna more to that specific compound. The significantly higher adaptation to Z7-12:OAc and Z9-14:OH than to the pheromone components of S. litura induced by themselves suggested that both of them are coded by specific odor receptor neurons which are different from those tuned to the pheromone components of S. litura. Thus, we proposed that Z7-12:OAc and Z9-14:OH may play an important role in avoidance of heterospecific mating between S. litura and its sympatric moth species.