• Journal of Practical Agriculture & Fisheries Research
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• v.12 no.1
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• pp.47-52
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• 2010

Squash cultivars of Cucurbita moschata have good fresh quality, but their viny growth habit hinders wide cultivation under structure. On the other hand, 'Zucchini' varieties of C. pepo have bush growth habit and better growth ability under the low temperature condition than C. moschata, but have inferior flesh quality. To combine desirable characteristics of the two species, many attempts have been exerted to obtain interspecific hybrids. A shootless phenomenon was found among segregating populations; the cotyledon expanded normally but further shoot growth. The inheritance of the character was investigated in F₂ and BC₁F₂ populations. The segregation ratios indicated that the shoot-lacking characteristics is expressed by double recessive gene pairs.

• The Plant Pathology Journal
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• v.40 no.4
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• pp.408-413
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• 2024

The emergence of rice black-streaked dwarf virus (RBSDV) poses a significant threat to global cereal crop cultivation, necessitating the urgent development of reliable detection and quantification techniques. This study introduces a reliable approach for the precise and sensitive quantification of the RBSDV in cereal crop samples, employing a reverse transcription digital polymerase chain reaction (RT-dPCR) assay. We assessed the specificity and sensitivity of the RT-dPCR assay proposed for precise RBSDV detection and quantification. Our findings demonstrate that RT-dPCR was specific for detection of RBSDV, with no cross-reactivity observed with other viruses infecting cereal crops. The RT-dPCR sensitivity was over 10 times that of RT-quantitative PCR (RT-qPCR). The detection limit of RT-dPCR was 0.096 copies/㎕. In addition, evaluation of RT-dPCR assay with field samples was conducted on 60 different cereal crop samples revealed that RT-dPCR (45/60) exhibited superior accuracy compared with RT-qPCR (23/60). In this study, we present a specific and accurate RT-dPCR assay for the detection and quantification of RBSDV.

• Journal of the Korean Society of International Agriculture
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• v.30 no.4
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• pp.300-304
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• 2018

'Bokha' is a new strawberry (Fragaria x ananassa Duch.) cultivar, which was released by the Highland Agriculture Research Institute in 2016. The 'Bokha' cultivar originated from a cross between 'Goha' and 'Saebong No. 3' that showed excellent ever-bearing characteristics, including continuous flowering habit and high soluble-solids contents under long-day and high temperature conditions in 2011. This cultivar was initially named 'Saebong No. 8' after examining its characteristics and productivity in summer culture from 2013 to 2015. After regional adaptability tests in 2016, 'Bokha' was selected from Saebong No. 8 as an elite cultivar. The general characteristics of 'Bokha' include semispreading type, elliptical leaves, and moderately vigorous growth. The fruits are conical in shape, and red in color. 'Bokha' plants have 32.7 leaves, 9.9 more than 'Goha' plants. The soluble-solids content of 'Bokha' was 9.2%, which was 0.2% higher than that of 'Goha'. The average fruit weight of 'Bokha' was about 9.5g and the marketable yield was $27,701kg{\cdot}ha^{-1}$, 72% higher than 'Goha'. 'Bokha' is suitable for tropical and subtropical zone cultivation as a high soluble solids contents cultivar, because it shows continuous flowering habit under long-day and high temperature conditions.

• Food Science and Preservation
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• v.18 no.4
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• pp.612-619
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• 2011

Cronobacter sakazakii has been isolated from a wide range of environmental sources and from several foods of animal and plant origin. The objective of this study was to determine the resistance of C. sakazakii (ATCC 12868, ATCC 29004, and ATCC 29544) in cold, cold-freeze thaw, cold-acid, and cold starvation-freeze thaw stress. The number of C. sakazakii decreased to 1 log CFU/mL at $5^{\circ}C$ (cold storage) for 10 days. When C. sakazakii was cultivated at a low temperature ($13^{\circ}C$), the population of C sakazakii ATCC 12868 and 29004 increased to $10^9$ CFU/mL, and the population of C. sakazakii ATCC 29544 increased to $10^8$ CFU/mL. For C. sakazakii ATCC 12868 and 29004, the cold-adapted cells ($5^{\circ}C$ 24 hr) decreased by 4 log CFU/mL, and the low-temperature-cultivated cells ($13^{\circ}C$) decreased by 0.5 log CFU/mL. In this study, low-temperature cultivation enhanced the freeze-thaw cross-resistance due to the metabolic changes in the cells. Cold stress ($5^{\circ}C$ 48 hr, $13^{\circ}C$ cultivation) enhanced the cold-acid cross-resistance. The cold-starved cells in the sterilized 0.1% peptone water enhanced the freeze-thaw cross-resistance with significant differences (p<0.05). Therefore, the increased tolerance of the cold-adapted or low-temperature-cultivated C. sakazakii cells to freeze-thaw, acid, or starvation suggests that such environments should be considered when processing minimally processed foods or foods with extended shelf life.

• Journal of Bio-Environment Control
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• v.22 no.1
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• pp.27-33
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• 2013

In order to provide basic data for the development of a controlled environment cultivation system and standardization of the structures, structural status and improvement methods were investigated for the fruit tree greenhouses of grape, pear, and peach. The greenhouses for citrus and grape cultivation are increasing while pear and persimmon greenhouses are gradually decreasing due to the advance of storage facilities. In the future, greenhouse cultivation will expand for the fruit trees which are more effective in cultivation under rain shelter and are low in storage capability. Fruit tree greenhouses were mostly complying with standards of farm supply type models except for a pear greenhouse and a large single-span peach greenhouse. It showed that there was no greenhouse specialized in each species of fruit tree. Frame members of the fruit tree greenhouses were mostly complying with standards of the farm supply type model or the disaster tolerance type model published by MIFAFF and RDA. In most cases, the concrete foundations were used. The pear greenhouse built with the column of larger cross section than the disaster tolerance type. The pear greenhouse had also a special type of foundation with the steel plate welded at the bottom of columns and buried in the ground. As the results of the structural safety analysis of the fruit tree greenhouses, the grape greenhouses in Gimcheon and Cheonan and the peach greenhouses in Namwon and Cheonan appeared to be vulnerable for snow load whereas the peach greenhouse in Namwon was not safe enough to withstand wind load. The peach greenhouse converted from a vegetable growing facility turned out to be unsafe for both snow and wind loads. Considering the shape, height and planting space of fruit tree, the appropriate size of greenhouses was suggested that the grape greenhouse be 7.0~8.0 m wide and 2.5~2.8 m high for eaves, while 6.0~7.0 m wide and 3.0~3.3 m of eaves height for the pear and peach greenhouses.

• Journal of Food Hygiene and Safety
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• v.30 no.1
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• pp.28-34
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• 2015

This study is to investigate microbiological hazards which can be used as fundamental data to adequately control leeks hazards and develop leeks GAP model for those who want to get GAP system. The microbiological investigations on cultivation environments (soil and water), crops (leeks), personal hygiene (workers' hands, clothes and gloves) and working tools (boxes) have been conducted for one year, so the period was classified under non-cultivation, cultivation, and post harvest. Total bacteria was detected from soil (4.0~6.7 log CFU/g), leeks (4.6~5.1 log CFU/g), hands (ND~3.3 log CFU/hand) and gloves ($ND{\sim}5.4\;log\;CFU/cm^2$) while nothing was detected from the other samples. The coliform contamination of leeks (4.8~5.0 log CFU/g) was more high than that of soil (3.9~4.2 log CFU/g). In case of foodborne pathogens, only B. cereus was detected at the level of 0.5~4.6 log CFU/g (or hand, $100cm^2$). Fungi was observed at the level of 2.1~3.8 log CFU/g (or hand, $100cm^2$) excepting water and some working tools. These results demonstrate that the contamination of leeks is comparatively higher than that of soil sample. The reason may be the cross-contamination by biological hazards presenting on soil. Therefore, it is necessary to properly control soil and fertilizer for safety against biological hazards.

• Journal of Life Science
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• v.30 no.9
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• pp.798-803
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• 2020

Late in the 1960s, South Korea established cultivation technology and began breeding new "Tongil" type rice cultivars by crossing indica and japonica. To date, this cultivation technique has been used to produce a wide variety of cultivars to meet consumer preferences and adapt to extreme weather conditions. Once major genetic traits are no longer segregated through advanced generations, varieties obtained from a cross between different crops may become a new variety. In this study, we confirmed the agronomic traits of F₄, F₅, and F₆ by advanced generation a population in which drought tolerance and brown planthopper (BPH) resistance were crossed. HV23, Ilmi/HV23, and Drimi2ho/HV23 were used. HV23 was drought-tolerant, and Drimi2ho was resistant to BPH. As a result, it was possible to consider that the agronomic traits were fixed because none of them showed a significant difference from the others. While it takes more than 10 years for the pedigree method of breeding, this study confirmed that the agronomic traits were fixed in 4-6 years. In the future, we will investigate the homology of the CaMsrB2 gene and the Bph1 gene, to confirm that both genes are closely related to each other, and analyze the stable inheritance of the introduced gene for multiple successive generations.

• Journal of Bio-Environment Control
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• v.22 no.3
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• pp.234-240
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• 2013

The purpose of this study was to provide basic data used in greenhouse design, and for this purpose, the crop loads of tomato and paprika which have high crop load among crops cultivated in greenhouse were measured. The maximum differences in lowest and mean temperatures between cultivation year and normal years for winter season were -5.6 and $-4.9^{\circ}C$, respectively and those for summer season were 3.0 and $1.5^{\circ}C$, respectively. The calibration of push & pull gauge indicated approximate 0.99 of coefficient of determination, representing very high association. The planting densities of paprika and tomato were 3.43, 3.56 and $2.13plants{\cdot}m^{-2}$, respectively. The yields were 4.1, 4.3 and $16.2kg{\cdot}plant^{-1}$, corresponding to 14.1, 15.2 and $34.5kg{\cdot}m^{-2}$ of yield per unit area. The maximum loads were 1.3, 1.5 and $3.3kg{\cdot}plant^{-1}$, respectively. When the paprika were hanged on cross beam, the total crop load applied to it was estimated to be $15.3{\sim}15.9kg{\cdot}m^{-2}$, and when the weights of gutters supporting the cultivation beds, the total weights were $26.0{\sim}26.9kg{\cdot}m^{-2}$.

• 한국균학회소식:학술대회논문집
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• 2009.10a
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• pp.3-13
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• 2009

90 wild Agaricus strains from China, including 44 Agaricus bisporus strains identified preliminarily by isozyme electrophoresis, were studied by the techniques of SRAP and ISSR. 18 special SRAP bands and 12 special ISSR bands were analyzed, the strains were clustered and a demdrogram was obtained. The results showed that the strains were divided into 2 groups, wild A. bisporus group and the other Agaricus group. It is similar to the result of isozyme electrophoresis. 41 wild A. bisporus strains from Sichuan and Tibet were divided into 4 groups based on their growing places, suggesting the regionally difference of the strains to be quite obvious. Some white wild A. bisporus strains from Xinjiang and Tibet had special patterns, resulting in lower coefficient values with other wild A. bisporus strains. The biological characteristics of three wild A. bisporus strains were analyzed, and the results showed: 1. The wild strains grew slowly on PDA medium with weak appressed mycelia, and grew normally in kernel or fermented cottonseed shell substrate. 2. They grew faster than control strain As2796 under lower temperature of $16^{\circ}C$, and higher temperature of $32^{\circ}C$, with optimum growing temperature of $20-24^{\circ}C$, which was $4^{\circ}C$ lower than that of control strain. 3. In the cultivation with manure compost via twice fermentation, the mycelia grew normally in compost and quite slowly in casing soil, and the fruitbodies occurred less and late with easily opening and low production. 4. The fruitbody was off-white with flat and scaled cap, long stipe and dark gill. The bisporus basidia occupied 70-80% and trisporus basidia 20-30% of the total basidia. 5. Heterokaryotic monospore isolates could fruit in cultivation, and the homokaryotic isolates could cross with those derived from overseas wild A.bisporus strains. 6. The electrophoresis phenotype of isozymes such as esterase etc. belonged to high production type (H type). 7. The RAPD patterns made much difference from those of high production, good quality or hybrid strains, which indicated that the wild strains produce a new kind of RAPD type.

• Journal of Mushroom
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• v.13 no.3
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• pp.243-249
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• 2015

Agaricus bitorquis is an edible white mushroom of the genus that is cultivated at high temperature($25{\pm}1^{\circ}C$) unlike A. bisporus is cultivate at $16{\pm}2^{\circ}C$. Unlike Agaricus bisporus, an edible white A. bitorquis mushroom is cultivated at high temperature ($25{\pm}1^{\circ}C$). Most farmers cultivate this mushroom for a long cultivation period in Korea. For this reason, we made heterokayons to develop a new cultivar that generate fruitbodies for short cultivation period. Over one hundred SSIs(single spores isolates) were collected from selected A. bitorquis ASI1151 and ASI1349 strains. Seventy-three SSIs were germinated on CDA(compost dextrose agar) media after 20 days (minimum) or 83 days (maximum) incubation under different media condition. The mycelial growth rate of germinated SSIs was different. 9 homokaryons in ASI 1151 and 11 homokaryons in ASI 1349 from SSIs were selected by OPN-02 primer in RAPD analaysis. Also this primer was used to select heterokaryon that cross among each homokaryon with compatible locus. Therefore 44 compatible matings were confirmed of 99 crossed lines.