• Food Science and Biotechnology
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• 제15권3호
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• pp.447-452
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• 2006

To determine the functions of specific cell wall polysaccharides, polysaccharides of three mutants, mur3-1, mur3-2, and mur3-3, obtained from Arabidopsis wild type, underwent structural characterization. Upon sequential separation of pectins (RG-I and RG-II) and cross-linking glycans (xyloglucan, XG), only XG was affected by the mud mutation. Wild-type XG contained a considerable amount of fucose, whereas the fucose level in mur3 XGs was less than 20% that of wild type. Further analysis of XGs by matrix-assisted laser-induced/ionization time-of-flight (MALDI-TOF) mass spectrometry indicated that mud lines considerably or completely lost the fucosylated XG oligosaccharides such as XXFG and XLFG and the double-galactosylated oligosaccharide XLLG $^1H$-NMR spectroscopic analyses of the XG oligosaccharides from mur3-3 plant revealed the absence of fucose and a galactose level in the galactosylated side chain that was reduced by 40% compared to that of Arabidopsis wild-type plant. In contrast, 85% less fucose and a slight loss of galactose were observed in the mur3-1 and mur3-2 lines which show normal growth habit. Of the three Arabidopsis mur3 lines studied here, mur3-3 is disrupted by a T-DNA insertion in the exon of MUR3 which encodes XG-specific galactosyltransferase, and exhibits slight dwarfism. These results indicated that the T-DNA insertion at the MUR3 locus did not induce the complete loss of galactose in XG, and that galactose, rather than fucose, in the XG side chains made a major contribution to overall wall strength.

• Nutrition Research and Practice
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• 제11권4호
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• pp.334-339
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• 2017

BACKGROUND/OBJECTIVES: Telomere length is a useful biomarker for determining general aging status. Some studies have reported an association between alcohol consumption and telomere length in a general population; however, it is unclear whether the alcohol flush reaction, which is an alcohol-related trait predominantly due to acetaldehyde dehydrogenase deficiency, is associated with telomere length. This cross-sectional study aimed to evaluate the associations of alcohol consumption and alcohol flush reaction with leukocyte telomere length (LTL). SUBJECTS/METHODS: The study included 1,803 Korean adults. Participants provided blood specimens for LTL measurement assay and reported their alcohol drinking status and the presence of an alcohol flush reaction via a questionnaire-based interview. Relative LTL was determined by using a quantitative polymerase chain reaction. Statistical analysis used multiple linear regression models stratified by sex and age groups, and potential confounding factors were considered. RESULTS: Age-specific analyses showed that heavy alcohol consumption (> 30 g/day) was strongly associated with a reduced LTL in participants aged ${\geq}65years$ (P < 0.001) but not in younger participants. Similarly, the alcohol flush reaction was associated with a reduced LTL only in older participants who consumed > 15 g/day of alcohol (P < 0.01). No significant alcohol consumption or alcohol flush reaction associations with LTL were observed in the sex-specific analyses. CONCLUSIONS: The results suggest that older alcohol drinkers, particularly those with the alcohol flush reaction, may have an accelerated aging process.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2006년도 가을 학술발표논문집 Vol.33 No.2 (A)
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• pp.53-58
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• 2006

MicroRNA (miRNA)는 약 22 nt의 작은 RNA 조각으로 이루어져 있으며 stem-loop 구조의 precursor 형태에서 최종적으로 만들어 진다. miRNA는 mRNA의 3‘UTR에 상보적으로 결합하여 유전자의 발현을 억제하거나 mRNA의 분해를 촉진한다. miRNA를 동정하기 위한 실험적인 방법은 조직 특이적인 발현, 적은 발현양 때문에 방법상 한계를 가지고 있다. 이러한 한계는 컴퓨터를 이용한 방법으로 어느 정도 해결될 수 있다. 하지만 miRNA의 서열상의 낮은 보존성은 homology를 기반으로 한 예측을 어렵게 한다. 또한 기계학습 방법인 support vector machine (SVM) 이나 naive bayes가 적용되었지만, 생물학적인 의미를 해석할 수 있는 generative model을 제시해 주지 못했다. 본 연구에서는 우수한 miRNA 예측을 보일 뿐만 아니라 학습된 모델로부터 생물학적인 지식을 얻을 수 있는 Bayesian network을 적용한다. 이를 위해서는 생물학적으로 의미 있는 특질들의 선택이 중요하다. 여기서는 position weighted matrix (PWM)과 Markov chain probability (MCP), Loop 크기, Bulge 수, spectrum, free energy profile 등을 특질로서 선택한 후 Information gain의 특질 선택법을 통해 예측에 기여도가 높은 특질 25개 와 27개를 최종적으로 선택하였다. 이로부터 Bayesian network을 학습한 후 miRNA의 예측 성능을 10 fold cross-validation으로 확인하였다. 그 결과 pre-/mature miRNA 각 각에 대한 예측 accuracy가 99.99% 100.00%를 보여, SVM이나 naive bayes 방법보다 높은 결과를 보였으며, 학습된 Bayesian network으로부터 이전 연구 결과와 일치하는 pre-miRNA 상의 의존관계를 분석할 수 있었다.

• 폴리머
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• 제35권6호
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• pp.610-614
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• 2011

본 연구에서는 방사선 전조사법으로 ETFE(polyethylene-co-tetrafluoroethylene) 불소고분자 필름에 VBC(vinylbenzyl chloride) 단량체를 그래프트할 경우 사용되는 용매의 영향을 평가하였다. ETFE 필름을 전자선을 조사하여 필름에 라디칼을 형성시킨 후 톨루엔, 햅탄, 이소프로판올 등 다양한 용매로 희석된 VBC 단량체 혼합물에 넣어 그래프트 반응을 진행시켜 그래프트율을 측정하였다. VBC-g-ETFE 필름은 FTIR 기기를 사용하여 성공적으로 그래프트 되었음을 확인하였다. 여러 용매에서 제조된 필름의 기계적 강도와 필름 단면에 그래프트된 VBC 고분자의 분포도를 측정하여 용매의 영향을 평가하였다.

• Elastomers and Composites
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• 제35권3호
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• pp.205-214
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• 2000

TDI에 PPG 3000을 기재로 사용하고 PPG 400, PPG 1000, PPG 2000을 $0{\sim}50%$ 혼합 사용하여 프리폴리머형 폴리우레탄수지를 합성한 후 합성 내용을 분석하고 합성물의 물성을 조사하였다. 반응은 촉매를 합성 초기에 넣었을 때에는 글리콜이 TDI 적하시에 대부분 반응되어 점도의 급상승이 일어나 불안하였지만 $40^{\circ}C$에서 무촉매 상태로 TDI를 적하시킨 후 $60^{\circ}C$ 승온 후에 촉매를 넣었을 때에는 매우 안정하였다. 인산을 사용하면 $40^{\circ}C$에서 부반응이 억제되어 점도는 낮아지지만 경화반응시 입체적으로 가교되지 못해 가교속도는 느리게 나타났다. 경화속도는 NCO/OH의 비보다는 분자의 입체적 구조에 더 영향을 받았다. 접착력은 글리콜 성분 중에 PPG 400을 적어도 30%(wt.%) 이상 사용하였을 때 PPG 400에 의한 가교밀도 상승으로 보다 우수한 물성을 나타내었다.

• 폴리머
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• 제34권4호
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• pp.369-375
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• 2010

용융 중합법을 이용하여 말단에 메틸 말레이미드(methyl maleimide) 반응성기를 가지며 방향족 에스터와 아 미드 결합을 포함하는 액정(liquid crystal, LC)을 합성하였다. 합성된 액정은 용액 캐스팅과 열처리를 거쳐 열경화 성 액정(liquid crystalline thermoset, LCT) 필름으로 제조되었다. 합성된 LC 및 LCT는 적외선 분광기(FTIR), 시차주사 열량계(DSC), 열중량 분석기(TGA), 열-기계 분석기(TMA), X-선 회절기(XRD) 및 가열판이 부착된 편광 현미경(POM) 등을 이용해서 각각 특성 분석을 하였다. 합성된 모든 액정은 스멕틱(smectic) 상을 보였으며 LC와 LCT들의 열적 성질은 주로 주사슬에 포함된 메소젠 구조에 영향을 받았다. 열팽창 계수(coefficients of thermal expansion, CTE)는 27.72~50.95 ppm/$^{\circ}C$ 사이의 값을 보였다.

• Journal of Veterinary Science
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• 제22권4호
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• pp.45.1-45.13
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• 2021

Background: Peste des petits ruminants (PPR) is an infectious disease caused by the peste des petits ruminants virus (PPRV) that mainly produces respiratory symptoms in affected animals, resulting in great losses in the world's agriculture industry every year. Single-domain variable heavy chain (VHH) antibody fragments, also referred to as nanobodies, have high expression yields and other advantages including ease of purification and high solubility. Objectives: The purpose of this study is to obtain a single-domain antibody with good reactivity and high specificity against PPRV. Methods: A VHH cDNA library was established by immunizing camels with PPRV vaccine, and the capacity and diversity of the library were examined. Four PPRV VHHs were selected, and the biological activity and antigen-binding capacity of the four VHHs were identified by western blot, indirect immunofluorescence, and enzyme-linked immunosorbent assay (ELISA) analyses. ELISA was used to identify whether the four VHHs were specific for PPRV, and VHH neutralization tests were carried out. ELISA and western blot analyses were used to identify which PPRV protein was targeted by VHH2. Results: The PPRV cDNA library was constructed successfully. The library capacity was greater than 2.0 × 10⁶ cfu/mL, and the inserted fragment size was approximately 400 bp to 2000 bp. The average length of the cDNA library fragment was about 1000 bp, and the recombination rate was approximately 100%. Four single-domain antibody sequences were selected, and proteins expressed in the supernatant were obtained. The four VHHs were shown to have biological activity, close affinity to PPRV, and no cross-reaction with common sheep diseases. All four VHHs had neutralization activity, and VHH2 was specific to the PPRV M protein. Conclusions: The results of this preliminary research of PPRV VHHs showed that four screened VHH antibodies could be useful in future applications. This study provided new materials for inclusion in PPRV research.

• Parasites, Hosts and Diseases
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• 제57권4호
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• pp.417-422
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• 2019

From October 2015 to August 2018, tapeworm proglottids were obtained from 10 patients who were residents of Daegu and Gyeongbuk provinces and had a history of raw beef consumption. Most of them had no overseas travel experience. The gravid proglottids obtained from the 10 cases had 15-20 lateral uterine branches. A part of internal transcribed spacer 1 (ITS1) DNA of the 10 cases, amplified by polymerase chain reaction (PCR) and digested with AleI restriction enzyme, produced the same band pattern of Taenia saginata, which differentiated from T. asiatica and T. solium. Sequences of ITS1 and cytochrome c oxidase subunit 1 (cox1) showed higher homology to T. saginata than to T. asiatica and T. solium. Collectively, these 10 cases were identified as T. saginata human infections. As taeniasis is one of the important parasitic diseases in humans, it is necessary to maintain hygienic conditions during livestock farming to avoid public health concerns.

• Clinical and Experimental Pediatrics
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• 제62권5호
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• pp.193-197
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• 2019

Alport syndrome (ATS) is an inherited glomerular disease caused by mutations in one of the type IV collagen novel chains (${\alpha}3$, ${\alpha}4$, and ${\alpha}5$). ATS is characterized by persistent microscopic hematuria that starts during infancy, eventually leading to either progressive nephritis or end-stage renal disease. There are 3 known genetic forms of ATS, namely X-linked ATS, autosomal recessive ATS, and autosomal dominant ATS. About 80% of patients with ATS have X-linked ATS, which is caused by mutations in the type IV collagen ${\alpha}5$ chain gene, COL4A5. Although an 80% mutation detection rate is observed in men with X-linked ATS, some difficulties do exist in the genetic diagnosis of ATS. Most mutations are point mutations without hotspots in the COL4A3, COL4A4, and COL4A5 genes. Further, there are insufficient data on the detection of COL4A3 and COL4A4 mutations for their comparison between patients with autosomal recessive or dominant ATS. Therefore, diagnosis of ATS in female patients with no apparent family history can be challenging. Therefore, in this study, we used whole-exome sequencing (WES) to identify mutations in type IV collagen in 2 girls with glomerular basement membrane structural changes suspected to be associated with ATS; these patients had no relevant family history. Our results revealed de novo c.4688G>A (p.Arg1563Gln) and c.2714G>A (p.Gly905Asp) mutations in COL4A5. Therefore, we suggest that WES is an effective approach to obtain genetic information in ATS, particularly in female patients without a relevant family history, to detect unexpected DNA variations.

• Journal of Genetic Medicine
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• 제16권1호
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• pp.39-42
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• 2019

KBG syndrome is an autosomal dominant syndrome presenting with macrodontia, distinctive facial features, skeletal anomalies, and neurological problems caused by mutations in the ankyrin repeat domain 11 (ANKRD11) gene. The diagnosis of KBG is difficult in very young infants as the characteristic macrodontia and typical facial features are not obvious. The youngest patient diagnosed to date was almost one year of age. We here describe a 2-month-old Korean boy with distinctive craniofacial features but without any evidence of macrodontia due to his very early age. He also had a congenital megacolon without ganglion cells in the rectum. A de novo deletion of exons 5-9 of the ANKRD11 gene was identified in this patient by exome sequencing and real-time genomic polymerase chain reaction. As ANKRD11 is involved in the development of myenteric plexus, a bowel movement disorder including a congenital megacolon is not surprising in a patient with KBG syndrome and has possibly been overlooked in past cases.