• Parasites, Hosts and Diseases
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• v.24 no.2
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• pp.145-158
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• 1986

This study was undertaken to purify cystic fluid (CF) antigen of Taenia solium metacestodes by affinity chromatogaphy using specific monoclonal antibody(McAb) and to characterize the antigenicity of the purified antigen. The hybridoma cell lines, prepared by fusion between mouse plasmacytoma and spleen cells from BALB/c mice immunized with CF, secreted antibodies reacting to various helminthic antigens. Majority of cell lines reacted to CF only but some also reacted to parenchymal antigen of T. solium metacestodes, adult T. saginata, sparganum, hydatid cystic fluid, Paragonimus westermani and Clonorchis sinensis, either in combination with CF, other antigens or independently. Cloned cells derived from monoclonal lines also produced antibodies reacting either to CF only or to other helminthes in combination or independently. These results indicated that CF of T. solium metacestodes contained proteins which possessed antigenic determinants not only specific to CF but also cross reactive with the afore-mentioned helminthes. CF of T. solium metacestodes was purified by affinity chromatography using the McAb which reacted to CF and parenchymal antigens. The affinity-purified antigen (A-Ag) and unbound pool CF (U-Ag) were separated. A-Ag showed 2 protein bands by disc-PAGE whereas CF exhibited 6 bands and U-Ag consisted of all bands CF had. The diagnostic significance of A-Ag was evaluated by ELISA in human neurocysticercosis and other helminthic and neurologic diseases. By A-Ag, the levels of the specific IgG antibody, as shown by absorbance in sera and CSF, were lower than those of CF and U-Ag. Accordingly, the sensitivity was about 70% of CF and U-Ag. However, the nonspecific positive reactions to CF and U-Ag, observed in sparganosis, T. saginata infection and paragonimiasis did not occur when A-Ag was used. These results indicated that the affinity-purified A-Ag had the higher specificity but the lower sensitivity as a diagnostic antigen in cysticercosis, probably because it only detected a single or limited numbers of monospecific antibodies among the diverse polyclonal antibodies produced in the patients with neurocysticercosis.

• Journal of the Korean Institute of Landscape Architecture
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• v.37 no.3
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• pp.46-53
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• 2009

Urbanization brings several changes to the natural environment. Its consequences can have a direct effect on climatic features, as in the Urban Heat Island Effect. One factor that directly affects the urban climate is the green area. In urban areas, vegetation is suppressed in order to accommodate manmade buildings and streets. In this paper we analyze the effect of green areas on the urban temperature in Seoul. The period selected for analysis was July 30th, 2007. The ground temperature was measured using Landsat TM satellite imagery. Land cover was calculated in terms of city area, water, bare soil, wet lands, grass lands, forest, and farmland. We extracted the surface temperature using the Linear Regression Model. Then, we did a regression analysis between air temperature at the Automatic Weather Station and surface temperature. Finally, we calculated the temperature decrease area and the population benefits from the green areas. Consequently, we determined that a green area with a radius of 500m will have a temperature reduction area of $67.33km^2$, in terms of urban area. This is 11.12% of Seoul's metropolitan area and 18.09% of the Seoul urban area. We can assume that about 1,892,000 people would be affected by this green area's temperature reduction. Also, we randomly chose 50 places to analysis a cross section of temperature reduction area. Temperature differences between the boundaries of green and urban areas are an average of $0.78^{\circ}C$. The highest temperature difference is $1.7^{\circ}C$, and the lowest temperature difference is $0.3^{\circ}C$. This study has demonstrated that we can understand how green areas truly affect air temperature.

• Journal of Korean Society of Forest Science
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• v.83 no.4
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• pp.486-497
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• 1994

The objective of this study was to examine the effects of air pollution on Pinus thunbergii forests surrounding Sasang industrial complex in Korea. The injury index, contents of chlorophyll and mineral elements, and concentrations of water soluble sulfur in needles were investigated at sample plots surrounding industrial complex and compared with those of control far from industrial complex. The results obtained are as follows ; 1. Discoloration of Pinus thunbergii needles was severe in the vicinity of industrial complex, and the older needle age classes was the more severe its injury appeared. Injury index was increased in the vicinity of industrial complex. 2. Water-soluble sulfur concentration was high in the vicinity of industrial complex at all of needle age classes, and those of all plots were higher than that of control. 3. Chlorophyll a contents were lower at surrounding industrial complex than that at control. It was supposed that chlorophyll a was destroyed by air pollutants. Total chlorophyll contents and content ratio of chlorophyll a to b were influenced by decrease of chlorophyll a contents. 4. Phosphorus contents in needles were decreased even in remoted regions with the increase of needle age classes. Colcium contents in needles were decreased near industrial complex at all needle ages classes. 5. There were negative correlation, between injury index and chlorophyll contents, injury index and calcium contents, and there were positive correlation between injury index and calcium contents. 6. Cluster analysis was carried out to divide the injured regions on sample plots. As a result of the analysis, there were devided 3 regions, severe regions(1-8 plots), medium regions(9, 10, 11, 12, 15, 17 plot), slight(13, 14, 16, 18, 19 plot and control). 7. The cross section of visible injured needle showed the destruction of mesophyll cell, sclerenchyma cell in the outside of resin duct and endodermis, partially.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.6
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• pp.718-724
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• 1999

This study was investigated the changes of the properties of matter such as the gel formation of the combined fish based on the mixed rate between the ocean jumbo squid and Alaska pollock surimi, and compared the relationships between the gel formation and water holding capacity. The changes of the gel formation based on 20 min fish grinding time and $2.5\%$ salt concentration according to the mixed rate was thought as the optimal addition limit. There was no significant function of gel product more than $20\%$ Jumbo squid meat. The more squid meat in the mixed meat could make the lower breaking stress but 7:3 rate of pollock : squid could retain breaking strain. The effect of the moisture content on mixed fish meat was studied and the drastic decrease of the gel formation and water holding capacity was indicated in $78\%$.

• Journal of Life Science
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• v.22 no.8
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• pp.1114-1119
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• 2012

Bacteria are frequently contaminated during the collection and processing procedures of boar semen. Of the contaminants, Stenotrophomonas (S.) maltophilia is a Gram-negative bacterium that is widely distributed in a variety of habitats. Although PCR assays have been developed for the detection of S. maltophilia, they cross-react with some species of Xanthomonas. In this study, we designed a primer set for the detection of S. maltophilia in order to target the chiA (GenBank accession no. NC_010943) gene. The specific PCR products were amplified from S. maltophilia only, not from other tested strains that are frequently found in semen. The detection limit of the PCR was $1.5{\times}10^3$ CFU/ml with pure-cultured S. maltophilia and $1.5{\times}10^4$ CFU/ml with S. maltophilia spiked in semen. Twenty-six (5.9%) S. maltophilia were isolated from 440 semen samples. The PCR results exhibited 98.9% agreement with a comparison of S. maltophilia isolation. Also, the sensitivity and specificity of the PCR were 100% and 98.7%, respectively. In the antimicrobial susceptibility test, S. maltophilia isolates were highly susceptible to enrofloxacin and florfenicol, while the majority of them were resistant to amoxicillin/clavulanic acid, apramycin, ceftiofur, penicillin, and spectinomycin. These results indicated that the PCR using the chiA gene was proven to be reliable and effective for the detection of S. maltophilia with high levels of sensitivity and specificity.

• Korean Journal of Microbiology
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• v.52 no.1
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• pp.10-17
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• 2016

Enzyme immunoassay to analyze specific binding activity of antibody to antigen uses horseradish peroxidase (HRP) or alkaline phosphatase (AP). Chemical methods are usually used for coupling of these enzymes to antibody, which is complicated and random cross-linking process. As results, it causes decreases or loss of functional activity of either antibody or enzyme. In addition, most enzyme assays use secondary antibody to detect antigen binding activity of primary antibody. Enzymes coupled to secondary antibody provide a binding signal by substrate-based color development, suggesting secondary antibody is required in enzyme immunoassay. Additional incubation time for binding of secondary antibody should also be necessary. More importantly, non-specific binding activity caused by secondary antibody should also be eliminated. In this study, we cloned AP isolated from Escherichia coli (E. coli) chromosome by PCR and fused to) hAY4 single-chain variable domain fragment (ScFv) specific to death receptor (DR4) which is a receptor for tumor necrosis factor ${\alpha}$ related apoptosis induced ligand (TRAIL). hAY4 ScFv-AP expressed in E. coli showed 73.8 kDa as a monomer in SDS-PAGE. However, this fusion protein shown in size-exclusion chromatography (SEC) exhibited 147.6 kDa as a dimer confirming that natural dimerization of AP by non-covalent association induced ScFv-AP dimerization. In several immunoassay such as ELISA, Western blot and immunocytochemistry, it showed antigen binding activity by color development of substrates catalyzed by AP directly fused to primary hAY4 ScFv without secondary antibody. In summary, hAY4 ScFv-AP fusion protein was successfully purified as a soluble dimeric form in E. coli and showed antigen binding activity in several immunoassays without addition of secondary antibody which sometimes causes time-consuming, expensive and non-specific false binding.

• Korean Journal of Agricultural and Forest Meteorology
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• v.9 no.2
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• pp.149-160
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• 2007

KoFlux Gwangneung Supersite comprises complex topography and diverse vegetation types (and structures), which necessitate complementary multi-disciplinary measurements to understand energy and matter exchange. Here, we report the results of this ongoing research with special focuses on carbon/water budgets in Gwangneung forest, implications of inter-dependency between water and carbon cycles, and the importance of hydrology in carbon cycling under monsoon climate. Comprehensive biometric and chamber measurements indicated the mean annual net ecosystem productivity (NEP) of this forest to be ${\sim}2.6\;t\;C\;ha^{-1}y^{-1}$. In conjunction with the tower flux measurement, the preliminary carbon budget suggests the Gwangneung forest to be an important sink for atmospheric $CO_2$. The catchment scale water budget indicated that $30\sim40%$ of annual precipitation was apportioned to evapotranspiration (ET). The growing season average of the water use efficiency (WUE), determined from leaf carbon isotope ratios of representative tree species, was about $12{\mu}mol\;CO_2/mmol\;H_2O$ with noticeable seasonal variations. Such information on ET and WUE can be used to constrain the catchment scale carbon uptake. Inter-annual variations in tree ring growth and soil respiration rates correlated with the magnitude and the pattern of precipitation during the growing season, which requires further investigation of the effect of a monsoon climate on the catchment carbon cycle. Additionally, we examine whether structural and functional units exist in this catchment by characterizing the spatial heterogeneity of the study site, which will provide the linkage between different spatial and temporal scale measurements.

• Restorative Dentistry and Endodontics
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• v.30 no.2
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• pp.79-85
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• 2005

The purpose of this study was to evaluate the obturation efficiency of a non-standardized gutta-percha cone in curved root canals prepared with 0.06 taper nickel-titanium instruments. Sixty simulated curved root canals in clear resin blocks were prepared with crown-down technique using 0.06 taper rotary $ProTaper^{TM}$and ProFile (Dentsply-Maillefer) until apical canal was size 30. Root canals were randomly divided into 4 groups of 15 blocks and obturated with cold-laterally compacted gutta-percha technique by using either a non-standardized size medium gutta-percha cone or an ISO-standardized size 30 one as a master cone. Gutta-percha area ratio were calculated at apical levels of 1, 3 and 5 mm using AutoCAD 2000 after cross-sectioning, and the data were analyzed with one-way and two-way ANOVAs and Duncan's multiple range test. Non-standardized size medium cone groups showed significantly higher gutta-percha area ratio than standardized cone groups at all apical levels (p < 0.01). Non-standardized cone groups used significantly less accessory cones than standardized cone groups (p < 0.01).

• Journal of Korean Society of Forest Science
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• v.85 no.2
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• pp.288-299
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• 1996

This is the basic study to investigate the amount of transpirational water loss in thrifty mature Quercus mongolica stand by the heat pulse method. The differences of heat pulse velocity by direction and depth, differences of heat pulse velocity by dominant, codominant and suppressed trees, diurnal changes of heat pulse velocity due to the change of leaf water potential, vapor pressure deficit and radiation, and sap flow path way in sapwood by dye penetration were measured in stems. Finally the amounts of daily and annual transpiration in stand were calculated by the heat pulse velocity. The results obtained were summarized as follows : 1. Relationship between heat pulse velocity(V) and sap flow rate(SFR) was obtained as a equation of SFR=1.37V. 2. The sap flow rate was high in the order of dominant, codominant, and suppressed trees. The daily heat pulse velocity changed with radiation, temperature and vapor pressure deficit. 3. The heat pulse velocity showed the similar diurnal variation as the leaf water potential change. 4. The heat pulse velocity showed the highest value in May(4.0cm/hr in average), the lowest one in July(2.9cm/hr in average). 5. The heat pulse velocity in the same stem presented the highest value in the northern direction, medium in western, and the lowest in southern and eastern. 6. The heat pulse velocity in stem was highest in 0.5cm, medium in 1.0cm, and lowest in 1.5cm depth from the surface of stem. 7. The sap flow path way in stem showed sectorial straight ascent pattern in four sample trees. 8. The amount of sap flow(SF) was presented as a equation of $SF=1.37A{\cdot}V$(A: the cross-sectional area of sapwood, V: heat pulse velocity), and especially SF was larger in dominant tree than codominant and suppressed trees. 9. The amount of daily transpiration was 5.6ton/ha/day, and its composition ratio was 72% at day and 28% at night. 10. The amount of stand transpiration per month was largest in May(168ton/ha/month), lowest in July(125ton/ha/month). The amount of stand transpiration per year was 839ton/ha/year.

• Journal of the Geological Society of Korea
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• v.54 no.5
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• pp.477-488
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• 2018

Fluid muds commonly occur in estuarine environments, but their ancient examples have rarely been studied in terms of depositional characteristics and processes. Cores of estuarine channel deposits of the Early Cretaceous McMurray Formation, Alberta, Canada show various mudstone layers that possess depositional characteristics of high clay-concentration flows. These mudstone layers are examined in detail through microscopic observation of thin sections and classified into three microfacies (<1 to 25 mm thick) on the basis of sedimentary texture and structures. Structureless mudstone (Microfacies 1) consists mainly of clay particles and contains randomly dispersed coarser grains (coarse silt to fine sand). This microfacies is interpreted as being deposited by cohesive mud flows, i.e., fluid muds, which possessed sufficient strength to support suspended coarser grains (quasi-laminar plug flow). Silt-streaked mudstone (Microfacies 2) mainly comprises mudstone with dispersed coarse grains and includes very thin, discontinuous silt streaks of coarse-silt to very-fine-sand grains. The texture similar to Microfacies 1 indicates that Microfacies 2 was also deposited by cohesive fluid muds. The silt streaks are, however, suggestive of the presence of intermittent weak turbulence under the plug (upper transitional plug flow). Heterolithic laminated mudstone (Microfacies 3) is characterized by alternation of relatively thick silt laminae and much thinner clay laminae. It is either parallel-laminated or low-angle cross-laminated, occasionally showing low-amplitude ripple forms. The heterolithic laminae are interpreted as the results of shear sorting in the basal turbulent zone under a cohesive plug. They may represent low-amplitude bed-waves formed under lower transitional plug flows. These three microfacies reflect a range of flow phases of fluid muds, which change with flow velocities and suspended mud concentrations. The results of this study provide important knowledge to recognize fluid-mud deposits in ancient sequences and to better understand depositional processes of mudstones.