• 한국발생생물학회지:발생과생식
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• 제5권2호
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• pp.101-106
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• 2001

Insulin과 tumor necrosis factor alpha(TNF-$\alpha$)에 의한 초기 배아 발생의 조절기작을 알아보고자 생쥐의 상실배를 대상으로 이들이 첨가된 배양액에서 형태발생, 세포증식을 조사하고, 포배에서 mitogen activated protein kinase(MAPK, ERK1/2)의 활성 변화에 미치는 영향을 조사하였다. Insulin은 상실배의 체외발생 및 포배내 할구 수를 대조군에 비해 유의하게 증가시켰으며, TNF-$\alpha$는 발생율을 유의하게 감소시켰다. Insulin은 TNF- $\alpha$에 의한 배아 발생율 감소를 완화하였다. TNF-$\alpha$는 농도에 의존적으로 MAPK 활성을 감소시켰으며, insulin은 포배에서 MAPK의 활성을 유의하게 증가시킨 반면 TNF-$\alpha$는 처리농도에 의존적으로 MAPK 활성을 감소시켰다. 50 ng/ml 농도의 TNF-$\alpha$를 전처리한 포배에서는 insulin에 의한 MAPK 활성의 증가가 저해되었다. 이러한 결과로부터 생쥐의 착상전 초기 배아 발생조절에 insulin과 TNF-$\alpha$ 사이에 MAPK를 경유하는 cross talk이 존재함을 확인하였고 insulin은 TNF-$\alpha$ 에 의한 배아의 손상을 억제하는 것으로 사료된다.

• Molecules and Cells
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• 제39권3호
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• pp.250-257
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• 2016

Abiotic stresses such as drought and low temperature critically restrict plant growth, reproduction, and productivity. Higher plants have developed various defense strategies against these unfavorable conditions. CaPUB1 (Capsicum annuum Putative U-box protein 1) is a hot pepper U-box E3 Ub ligase. Transgenic Arabidopsis plants that constitutively expressed CaPUB1 exhibited drought-sensitive phenotypes, suggesting that it functions as a negative regulator of the drought stress response. In this study, CaPUB1 was over-expressed in rice (Oryza sativa L.), and the phenotypic properties of transgenic rice plants were examined in terms of their drought and cold stress tolerance. Ubi:CaPUB1 T3 transgenic rice plants displayed phenotypes hypersensitive to dehydration, suggesting that its role in the negative regulation of drought stress response is conserved in dicot Arabidopsis and monocot rice plants. In contrast, Ubi:CaPUB1 progeny exhibited phenotypes markedly tolerant to prolonged low temperature ($4^{\circ}C$) treatment, compared to those of wild-type plants, as determined by survival rates, electrolyte leakage, and total chlorophyll content. Cold stress-induced marker genes, including DREB1A, DREB1B, DREB1C, and Cytochrome P450, were more up-regulated by cold treatment in Ubi:CaPUB1 plants than in wild-type plants. These results suggest that CaPUB1 serves as both a negative regulator of the drought stress response and a positive regulator of the cold stress response in transgenic rice plants. This raises the possibility that CaPUB1 participates in the cross-talk between drought and low-temperature signaling pathways.

• 대한전자공학회논문지SD
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• 제48권6호
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• pp.39-44
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• 2011

본 논문은 배터리로부터 입력받은 제한된 전압 값을 변환하여 PMIC의 출력 전압 값에 대한 PCB 패턴의 두 가지 모드로 설계한 PCB 패턴의 특성분석을 제안하고자 한다. PCB 설계 기술은 EMI/EMC, 크로스토크, 임피던스 증가 등으로 국내에서는 관련 기술의 미확보로 많은 어려움을 겪고 있으며, 사용되는 용도에 따라 패턴 상호간의 적절한 이격거리 확보, 전류량에 따른 PCB 패턴 폭 등의 기술이 요구되고 있다. 본 논문에서는 PMIC의 출력 전압 값을 커패시터를 거치지 않고 직접 출력하는 방법[모드1]과 커패시터를 거쳐 출력하는 방법[모드2]으로 설계하여 PCB 패턴의 특성 분석을 수행하였다. 또한 IPC-2221에서 제시한 식을 이용하여 전류량에 따른 패턴 폭을 계산해 보았으며, 실험을 통한 패턴 설계에 따른 문제점을 분석하여 올바른 설계 방향을 제시하였다. 이러한 연구는 비단 모바일 패널 전원 공급을 위한 PMIC 설계에 적용될 뿐만 아니라 자동차, 카메라, 노트북, PDA 등의 다양한 분야의 전력 반도체 개발에 적용될 수 있을 것으로 기대된다.

• 한국전자파학회논문지
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• 제18권5호
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• pp.563-568
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• 2007

본 논문에서 제안된 패키지 안테나는 900 MHz RFID 대역에 적용하기 위하여 미엔더 라인과 단락 핀을 부설하고 LTCC(Low Temperature Cofired Ceramic) 층의 공동 내부에 RFID BGA(Ball Grid Array) 칩을 장착하였다. BGA 칩과 안테나 사이에 발생되는 커플링과 혼신을 감소시키기 위한 격리 특성을 확보하기 위하여 접지면과 비아 펜스를 부설하여 Faraday Cage를 구현하였다. 제안된 안테나는 낮은 주파수 대역에서 패키지 단계의 안테나 구현에 관점을 두었다. 제안된 안테나의 크기는 $13mm{\times}9mm{\times}3.51mm$이며, 측정된 안테나의 공진 주파수는 0.893 GHz, 임피던스 대역폭은 9 MHz, 최대 방사 이득은 -2.36 dBi를 나타내었다.

• Molecules and Cells
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• 제43권1호
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• pp.23-33
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• 2020

NF-κB signaling through both canonical and non-canonical pathways plays a central role in immune responses and inflammation. NF-κB-inducing kinase (NIK) stabilization is a key step in activation of the non-canonical pathway and its dysregulation implicated in various hematologic malignancies. The tumor suppressor, p53, is an established cellular gatekeeper of proliferation. Abnormalities of the TP53 gene have been detected in more than half of all human cancers. While the non-canonical NF-κB and p53 pathways have been explored for several decades, no studies to date have documented potential cross-talk between these two cancer-related mechanisms. Here, we demonstrate that p53 negatively regulates NIK in an miRNA-dependent manner. Overexpression of p53 decreased the levels of NIK, leading to inhibition of the non-canonical NF-κB pathway. Conversely, its knockdown led to increased levels of NIK, IKKα phosphorylation, and p100 processing. Additionally, miR-34b induced by nutlin-3 directly targeted the coding sequences (CDS) of NIK. Treatment with anti-miR-34b-5p augmented NIK levels and subsequent non-canonical NF-κB signaling. Our collective findings support a novel cross-talk mechanism between non-canonical NF-κB and p53.

• 생명과학회지
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• 제12권2호
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• pp.164-172
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• 2002

유방암세포는 여러 종류의 성장인자 수용체를 가지며, 이를 통해 성장신호를 전달한다. 따라서, 이러한 수용체들의 신호전달 경로에 있는 공통적인 2차전달자들의 조절기작을 밝히는 것이 중요하다. 이 연구는 MCF-7 cell에 있어서, 에스트로젠과 TGF-$\alpha$ , EGF와 같은 성장인자의 mitogenic signal을 전달하는 second messenger에 폴리아민이 어떤 영향을 미치는지, 또 membrane-associated proteins의 인산화에 폴리아민이 어떤 조절기작을 가지는지를 알아보고자 한다. 폴리아민 생합성 억제제인 DFMO는 154, 134, 116, 104 kDa의 membrane-associated proteins의 인산화를 억제하였고, DFMO에 의해 억제된 단백질 인산화는 폴리아민 첨가로 다시 회복 되었다. $E_2$, TGF-$\alpha$, EGF, DFMO는 모두 단백질의 타이로신 인산화에는 크게 영향을 미치지 않았으나, 처리해준 폴리아민에 의해 154, 134, 116 kDa의 단백질의 타이로신 인산화는 급격히 증가하였다. 또한 $E_2$, TGF-$\alpha$, EGF는 모두 같은 단백질에서 유사하게 인산화를 유도하였다. 이러한 결과로 볼 때, $E_2$와 TGF-$\alpha$, EGF와 같은 성장촉진인자의 signaling pathway는 154, 134, 116, 104 kDa 단백질을 기질로 하는 여러 가지 다양한 종류의 protein kinase를 통해서 서로 cross-talk하고 있으며, 폴리아민은 154, 134, 116, 104 kDa와 같은 여러 가지 membrane-associated proteins의 인산화를 조절함으로서 이러한 cross-talk pathway에 관여하고 있는 것으로 사려된다.

• BMB Reports
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• 제38권1호
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• pp.9-16
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• 2005

Transforming Growth Factor (TGF)-$\beta$ family, including TGF-$\beta$, bone morphorgenic protein (BMP), and activn, plays an important role in essential cellular functions such as proliferation, differentiation, apoptosis, tissue remodeling, angiognesis, immune responses, and cell adhesions. TGF-$\beta$ predominantly transmits the signals through serine/threonine receptor kinases and cytoplasmic proteins called Smads. Since the discovery of TGF-$\beta$ in the early 1980s, the dysregulation of TGF-$\beta$/Smad signaling has been implicated in the pathogenesis of human diseases. Among signal transducers in TGF-$\beta$/Smad signaling, inhibitory Smads (I-Smads), Smad6 and Smad7, act as major negative regulators forming autoinhibitory feedback loops and mediate the cross-talking with other signaling pathways. Expressions of I-Smads are mainly regulated on the transcriptional levels and post-translational protein degradations and their intracellular levels are tightly controlled to maintain the homeostatic balances. However, abnormal levels of I-Smads in the pathological conditions elicit the altered TGF-$\beta$ signaling in cells, eventually causing TGF-$\beta$-related human diseases. Thus, exploring the molecular mechanisms about the regulations of I-Smads may provide the therapeutic clues for human diseases induced by the abnormal TGF-$\beta$ signaling.

• 무역학회지
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• 제45권1호
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• pp.155-171
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• 2020

In reality, there are numerous [Note: I advise to change the tone to being more certain, as opposed to offering a possibility. You need to emphasize the seriousness of the conditions that your study wants to talk about] non-tariff trade barriers under free trade agreements, with various stakeholders having different trade-off interests. This study focuses on the rules of origin verification and considers cases in which domestic firms, foreign firms, and domestic consumer groups compete politically for their rent protection in the domestic market. As in Gwande et al. (2006), cross-border lobbies are considered possible wherein foreign firms also lobby the government to influence the decision-making process of how stringently to verify the rules of origin. [Note: Starting here, switch to past tense because a: study intentions above can be in present tense, b: study procedures should be in past tense] In this study, we assumed that all stakeholders form interest groups and present political contributions to the government based on their interests. The stringency of the rules of origin verification was then determined through this political process, and we compared this to a socially optimal one in the analysis. It was found that the verification was less stringent when there was cross-border lobbying than the socially optimal one.

• 대한전기학회논문지:시스템및제어부문D
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• 제51권9호
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• pp.423-430
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• 2002

The fabrication and experimentation of multi-channel electrodes which enable detecting and recording of multi-site neuronal signals have been investigated. A multi-channel electrode array was fabricated by depositing 2000${\AA}$ thick Au layer on the 1000${\AA}$ thick Ti adhesion layer on a glass wafer. The metal paths were patterned by wet etching and passivated by depositing a PECVD silicon nitride insulation layer to prevent signals from intermixing or cross-talking. After placing a thin slice of rat cerebellar granule cell in the culture ring located in central portion of the multi-channel electrode plate, a neuronal signal from an electrode which is in contact with the cerebellar granule cell has been detected. It was found that the electrode impedance ranges 200㏀∼1㏁ and the impedance is not changed by cleaning with nitric acid. Also, the impedance is inversely proportion to the exposed electrode area and the cross-talk is negligible when the electrode spacing is bigger than 600$\mu\textrm{m}$. The amplitude and frequency of the measured action potential were 38㎷ and 2㎑, which are typical values. From the experimental results, the fabricated multi-channel electrode array proved to be suitable for multi-site neuronal signal detection for the analysis of a complicated cell network.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제13권5호
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• pp.2258-2276
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• 2019

Resource sharing is one of the main goals achieved by network virtualization technology to enhance network resource utilization and enable resource customization. Though resource sharing can improve network efficiency by accommodating various users in a network, limited infrastructure capacity is still a challenge to ultra-low latency service operators. In this paper, we propose an inter-slice resource borrowing schema based on the device-to-device (D2D) potentiality especially for ultra-low latency transmission in cellular networks. An extended and modified Kuhn-Munkres bipartite matching algorithm is developed to optimally achieve inter-slice resource borrowing. Simulation results show that, proper D2D user matching can be achieved, satisfying ultra-low latency (ULL) users' quality of service (QoS) requirements and resource utilization in various scenarios.