• Journal of Microbiology and Biotechnology
• /
• v.29 no.9
• /
• pp.1470-1477
• /
• 2019

${\beta}_2$-adrenergic receptor (${\beta}_2-AR$) was expressed efficiently using Bac-to-Bac Baculovirus Expression System in Sf9 cells as a bio-recognition element for multianalyte screening of ${\beta}$-agonist residues in pork. Sf9 cells were selected as the expression system, and codon optimization of wild-type nucleic acid sequence and time-dependent screening of expression conditions were then carried out for enhancing expression level and biological activity. Under optimum conditions of multiplicity of infection (MOI) = 5 and 48 h post transfection, the protein yield was up to 1.23 mg/ml. After purification by chromatographic techniques, the purified recombinant protein was applied to develop a direct competitive enzyme-linked receptor assay (ELRA) and the efficiency and reliability of the assay was determined. The IC50 values of clenbuterol, salbutamol, and ractopamine were 28.36, 50.70, and $59.57{\mu}g/l$, and clenbuterol showed 47.61% and 55.94% cross-reactivities with ractopamine and salbutamol, respectively. The limit of detection (LOD) was $3.2{\mu}g/l$ and the relevant recoveries in pork samples were in the range of 73.0-91.2%, 69.4-84.6%, and 63.7-80.2%, respectively. The results showed that it had better performance compared with other present nonradioactive receptorbased assays, indicating that the genetically modified ${\beta}_2-AR$ would have great application potential in detection of ${\beta}$-agonist residues.

• Steel and Composite Structures
• /
• v.32 no.4
• /
• pp.479-495
• /
• 2019

In tied-arch bridges, the way the arch and the deck are connected may become crucial. The deck is usually suspended from hangers made out of steel pinned cables capable of resisting axial forces only. However, a proper structural response may be ensured by fixing and stiffening the hangers in order to resist, additionally, shear forces and bending moments. Thus, this paper studies the effect of different pinned and stiffened hanger arrangements on the structural behavior of the tied-arch bridges, with the intention of providing designers with useful tools at the early steps of design. Longitudinally and transversally stiffened hangers (and the effect of hinges at the hangers and their locations) are studied separately because the in-plane and the out-of-plane behavior of the bridge are uncoupled due to its symmetry. As a major conclusion, regarding the in-plane behavior, hangers composed of cables (either with vertical, $Nielsen-L\ddot{o}hse$ or network arrangements) are recommended due to its low cost and ease of erection. Alternatively, longitudinally stiffened hangers, fixed at both ends, can be used. Regarding the out-of-plane behavior, and in addition to three-dimensional arrangements of cables, of limited effectiveness, transversally stiffened hangers fixed at both ends are the most efficient arrangement. A configuration almost as efficient and, additionally, cheaper and easier to build can be achieved by locating a hinge at the end corresponding to the most flexible structural element (normally the arch). Its efficiency is further improved if the cross-section tapers from the fixed end to the pinned end.

• Journal of Korea Port Economic Association
• /
• v.35 no.1
• /
• pp.77-96
• /
• 2019

Logistics and transportation are increasingly playing a crucial role in international trade relations. Logistics services provide sectoral connections within the local economy. The efficient logistics services facilitate the flow of products; besides ensuring safety and speed of movement, they can reduce the cost of cross-border trade. The Logistics Performance Index (LPI) evaluates the efficiency of customs, quality of transportation service, and infrastructure for overland and maritime transport in each country it covers. The objective of this article is to analyze the impact of the various LPI indicators of Korea's trading partners. The impact of LPI on trade varies according to the income level of the trading partners. The results show that an improvement in any of the components of the LPI can lead to significant growth in a country's trade flow. Exports to Korea will increase as the LPI of the trading partner country is higher. This index has a greater impact on the exports of low-income countries.

• Journal of Hydrogen and New Energy
• /
• v.24 no.2
• /
• pp.150-159
• /
• 2013

To enhance the performance of SEWGS system by holding the WGS catalyst in a SEWGS reactor using bed inserts, effects of insert geometry and shape of WGS catalysts on CO conversion were measured and investigated. Small scale fluidized bed reactor was used as experimental apparatus and WGS catalyst (particle and tablet) and sand were used as bed materials. The parallel wall type and cross type bed inserts were used to hold the WGS catalysts. The CO conversion with steam/CO ratio was determined based on the exit gas analysis. The measured CO conversion using the bed inserts showed high value comparable to physical mixing cases. Moreover, gas flow direction was confirmed by bed pressure drop measurement for each case. Most of input gas flowed through the catalyst side when we charged tablet type catalyst into the bed insert and this can cause low $CO_2$ capture efficiency because the possibility of contact between input gas and $CO_2$ absorbent is low in this case. New bed insert geometry was proposed based on the results from this study to enhance contact between input gas and WGS catalyst and $CO_2$ absorbent.

• Physical Therapy Rehabilitation Science
• /
• v.10 no.1
• /
• pp.10-15
• /
• 2021

Objective: Limited ankle dorsiflexion is related to ankle injuries. There are various exercises to increase the flexibility of the gastrocnemius for improving the passive range of motion in ankle dorsiflexion. However, to performances in daily activities and athletic sports and higher efficiency of walking and running, both ankle dorsiflexion passive and active range of motion are needed. To investigate the effects of combined gastrocnemius stretching and tibialis anterior resistance exercise on ankle kinematics (passive and active range of motion of ankle dorsiflexion) and tibialis anterior muscle activity in subjects with limited ankle dorsiflexion. Design: Cross-sectional single-group repeated measures design. Methods: Fourteen subjects with limited ankle dorsiflexion were recruited (in the right ankle in 7 and the left ankle in 7). All subjects performed gastrocnemius stretching alone and tibialis anterior resistance exercise after gastrocnemius stretching. The passive and active range of motion of ankle dorsiflexion were measured after interventions immediately. The tibialis anterior activity was measured during active range of motion of ankle dorsiflexion measurement. Results: There was no significant difference of ankle dorsiflexion passive range of motion between gastrocnemius stretching alone and the tibialis anterior resistance exercise after gastrocnemius stretching. The tibialis anterior resistance exercise after gastrocnemius stretching significantly increased active range of motion of ankle dorsiflexion compared to gastrocnemius stretching alone (p<0.05). The tibialis anterior resistance exercise after gastrocnemius stretching significantly increased tibialis anterior activity better than did gastrocnemius stretching alone. Conclusions: Thus, subjects with limited ankle dorsiflexion should be encouraged to perform tibialis anterior resistance exercises.

• Journal of Distribution Science
• /
• v.20 no.2
• /
• pp.65-77
• /
• 2022

Purpose: This study aims to identify factors affecting the adoption of e-commerce by household businesses in Vietnam's food and beverage industry. Research design, data and methodology: The integrated TAM and TOE frameworks and ten hypotheses were developed to test the relationship between relevant factors. A cross-sectional survey was conducted to collect valid data from 992 respondents who run F&B household businesses in Vietnam. Results: The empirical research results confirm all proposed hypotheses that e-commerce adoption is affected by the perceived usefulness, the perceived ease of use, vendor support partners, social expectancy, competitive pressure, subjective norm, and household resources. Meanwhile, technical readiness, environment readiness, and organization readiness are intermediate variables that influence the implementation of e-commerce in food and beverage distribution. Conclusions: The findings suggest effective orientations to foster the food and beverage e-trading practice for household businesses in developing countries. Accordingly, to encourage private household businesses to adopt e-commerce, it is necessary to focus on improving business resources, technology readiness, environment readiness, and organization readiness through raising awareness of usefulness, benefits, perceived ease of use, and increased support of vendor partners. Future research can focus on improving the efficiency of e-commerce applications in F&B distribution for both household businesses and larger-scale enterprises.

• Journal of The Korean Society of Integrative Medicine
• /
• v.9 no.2
• /
• pp.43-52
• /
• 2021

Purpose : Chronic neck pain negatively impacts the quality of life and causes various problems in daily life due to pain, insomnia, and sleep disturbances in patients with this condition. Therapeutic interventions to solve these problems in rehabilitation and physical therapy are being introduced; however, the evidence of the efficacy of myofascial release (MFR) is still insufficient. This study aimed to investigate the effects of applying MFR on pain, insomnia, and sleep disturbances in patients with chronic neck pain. Methods : Ten patients with chronic neck pain were randomly selected and grouped into the experimental group (n₁ = 10) and control group (n₂ = 10) by cross-over design. Pain was measured before and after MFR intervention. Moreover, insomnia was measured only after MFR intervention. Polysomnography was performed after MFR intervention. Wilcoxon signed rank test and Mann-Whitney U test were used for the visual analog scale (VAS). Independent sample t-test was separately performed to measure insomnia and sleep. Results : After MFR intervention, the VAS score of the experimental group (p = 0.005) significantly decreased than that of the control group (p = 0.002). The insomnia score of the experimental group significantly decreased than that of the control group (p = 0.001). The total sleep time (p = 0.001), sleep efficiency (p = 0.001), and sleep latency (p = 0.001) of the experimental group significantly increased than those of the control group in the polysomnographic measurement. Conclusion : The application of MFR of the neck and upper trunk may have a positive effect on pain, insomnia, and sleep disturbances in patients with chronic neck pain. It was also suggested that an objective and quantitative polysomnography can be used more often in the field of rehabilitation and physical therapy.

• Journal of Veterinary Science
• /
• v.23 no.5
• /
• pp.78.1-78.13
• /
• 2022

Background: Florfenicol might be ineffective for treating Staphylococcus aureus small colony variants (SCVs) mastitis. Objectives: In this study, florfenicol-loaded chitosan (CS)-sodium tripolyphosphate (TPP) composite nanogels were prepared to allow targeted delivery to SCV infected sites. Methods: The formulation screening, the characteristics, in vitro release, antibacterial activity, therapeutic efficacy, and biosafety of the florfenicol composite nanogels were studied. Results: The optimized formulation was obtained when the CS and TPP were 10 and 5 mg/mL, respectively. The encapsulation efficiency, loading capacity, size, polydispersity index, and zeta potential of the optimized florfenicol composite nanogels were 87.3% ± 2.7%, 5.8% ± 1.4%, 280.3 ± 1.5 nm, 0.15 ± 0.03, and 36.3 ± 1.4 mv, respectively. Optical and scanning electron microscopy showed that spherical particles with a relatively uniform distribution and drugs might be incorporated in cross-linked polymeric networks. The in vitro release study showed that the florfenicol composite nanogels exhibited a biphasic pattern with the sustained release of 72.2% ± 1.8% at 48 h in pH 5.5 phosphate-buffered saline. The minimal inhibitory concentrations of commercial florfenicol solution and florfenicol composite nanogels against SCVs were 1 and 0.25 ㎍/mL, respectively. The time-killing curves and live-dead bacterial staining showed that the florfenicol composite nanogels were concentration-dependent. Furthermore, the florfenicol composite nanogels displayed good therapeutic efficacy against SCVs mastitis. Biological safety studies showed that the florfenicol composite nanogels might be a biocompatible preparation because of their non-toxic effects on the renal tissue and liver. Conclusions: Florfenicol composite nanogels might improve the treatment of SCV infections.

• Membrane Journal
• /
• v.32 no.4
• /
• pp.209-217
• /
• 2022

Ion exchange membrane (IEM) is an important class of membrane applied in batteries, fuel cells, chloride-alkali processes, etc to separate various mono and multivalent ions. The membrane process is based on the electrically driven force, green separation method, which is an emerging area in desalination of seawater and water treatment. Electrodialysis (ED) is a technique in which cations and anions move selectively along the IEM. Anion exchange membrane (AEM) is one of the important components of the ED process which is critical to enhancing the process efficiency. The introduction of cross-linking in the IEM improves the ion-selective separation performance due to the reduction of free volume. During the desalination of seawater by reverse osmosis (RO) process, there is a lot of dissolved salt present in the concentrate of RO. So, the ED process consisting of a monovalent cation-selective membrane reduces fouling and improves membrane flux. This review is divided into three sections such as electrodialysis (ED), anion exchange membrane (AEM), and cation exchange membrane (CEM).

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.17 no.2
• /
• pp.391-411
• /
• 2023

Aggregates play the skeleton and supporting role in the construction field, high-precision measurement and high-efficiency analysis of aggregates are frequently employed to evaluate the project quality. Aiming at the unbalanced operation time and segmentation accuracy for multi-class segmentation algorithms of aggregate images, a Chaotic Sparrow Search Algorithm (CSSA) is put forward to optimize it. In this algorithm, the chaotic map is combined with the sinusoidal dynamic weight and the elite mutation strategies; and it is firstly proposed to promote the SSA's optimization accuracy and stability without reducing the SSA's speed. The CSSA is utilized to optimize the popular multi-class segmentation algorithm-Multiple Entropy Thresholding (MET). By taking three METs as objective functions, i.e., Kapur Entropy, Minimum-cross Entropy and Renyi Entropy, the CSSA is implemented to quickly and automatically calculate the extreme value of the function and get the corresponding correct thresholds. The image adaptive multi-class segmentation model is called CSSA-MET. In order to comprehensively evaluate it, a new parameter I based on the segmentation accuracy and processing speed is constructed. The results reveal that the CSSA outperforms the other seven methods of optimization performance, as well as the quality evaluation of aggregate images segmented by the CSSA-MET, and the speed and accuracy are balanced. In particular, the highest I value can be obtained when the CSSA is applied to optimize the Renyi Entropy, which indicates that this combination is more suitable for segmenting the aggregate images.