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A Qualitative Study of the Aging Experience among Korean Older Adults Residing in Urban, Rural, and the US Immigrant Context (도시, 농촌, 재미 이민사회에 거주하는 한국노인의 노화 경험에 관한 질적 연구)

  • Lee, Jun-woo;Pak, Jenny H;Lee, Hyuna
    • 한국노년학
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    • v.39 no.3
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    • pp.589-612
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    • 2019
  • Korea has undergone rapid modernization and globalization process in the last few decades. The purpose of this study is to explore how shifting traditional values are impacting Korean elderlies and their perceptions of aging experience. In order to understand how changing social and cultural practices are affecting older adults, the similarities and differences among Korean elderlies living in three different geographical locations are examined in a multidimensional comparative framework. Life story interviews were conducted with 30 Korean elderlies (10 urban residents in Korea, 10 rural residents in Korea, and 10 residents in the US). Paying attention to within-group variation, this study particularly focused on analyzing the psychological, social, emotional, and spiritual experiences that made lives meaningful and resilient in old age in spite of physical decline. Based on analysis of narrative, the following major areas were identified as having positive or negative influence on aging experiences: physical health, emotional health, family relationship, work, leisure/social activity, and spirituality. Common themes of aging well across all settings included: "life not indebted to their offspring," "physical and mental health," and "dying well." Spirituality played a critical role in cultivating perspectives on life and accepting aging process, which were rooted in specific religious traditions participants identified (e.g., Christianity or Buddhism). Interesting differences in the meaning of work and leisure were also found between elderlies residing in Korea and the US immigrant context. One unique factor continuing to negatively affect Korean elderly in rural community was related to shame of not obtaining higher education. Implications for future research are also addressed.

Exploratory Study on the Efficient Operation of Parcel Delivery Network with the Growth of Online Shopping Industries (온라인 쇼핑의 성장에 따른 택배물류 네트워크의 효율적 운영에 관한 탐색적 연구)

  • Lim, Hyunwoo;Lim, Jong Won;Yi, Hansuk
    • Asia Marketing Journal
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    • v.9 no.2
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    • pp.97-129
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    • 2007
  • The critical link between consumer-based internet ordering and the delivery of the product to the consumer is a key success factor in online shopping. Market areas of online shopping company implies the range of space where products ordered from online shopping can be physically delivered to customers distributed over space with reasonable shipping cost and lead time through the physical distribution network. The average rate of growth in online shopping is 36% per year in Korea for the last 5 years. But there are no maps available that describe sales/delivery density of online shopping, few researches are focused on the short-term/long-term adaptation to demand increase by online shopping. In this paper (1) Maps of trade area are described indicating the sales/delivery density around the nation. (2) Empirical researches suggested that short-term adaptation to demand increase resulted in price reduction and service in enhancement of service quality in local transportation. But the long-term adaptation on the parts of parcel delivery industry are to be investigated in future researches.

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The Influence of Dynamic Capabilities on the Structural Relationship between Accelerator Programs and Startup Performance (액셀러레이터 프로그램과 스타트업 성과의 구조적 관계에서 동적역량의 영향 )

  • Choi, Bo-ra;Dong, Hak-rim
    • Journal of Venture Innovation
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    • v.6 no.1
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    • pp.95-114
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    • 2023
  • As the Fourth Industrial Revolution progresses, the importance of startups is becoming more prominent. Additionally, the role of accelerators in ensuring the sustainability of startups is also being emphasized. Therefore, in this study, among the services provided by accelerators to startups, the mentoring service, education service, funding service, and networking service tried to empirically analyze the structural relationship that affects startup performance through the dynamic capabilities of start-ups. To achieve this, we conducted a survey in February 2023 targeting startup CEOs and executives who had experience with accelerator program services. A total of 238 valid responses were analyzed using structural equation modeling with Amos 24. The results of the empirical analysis were as follows. The accelerator program services, including mentoring, education, funding, and networking services, had a significant positive effect on startup dynamic capabilities. Among the accelerator program sub-factors, networking services had the highest impact on startup dynamic capabilities, followed by mentoring, funding, and education services. Furthermore, startup dynamic capabilities were found to have a significant positive effect on startup performance. On the other hand, startup dynamic capabilities were found to mediate the relationship between mentoring, education, funding, networking services, and startup performance. Dynamic capabilities are a critical factor for startups, and the finding that accelerator programs can have a significant impact on startup performance by mediating these dynamic capabilities is significant both academically and practically.

Study of Small Craft Resistance under Different Loading Conditions using Model Test and Numerical Simulations (모형시험과 수치해석을 이용한 하중조건 변화에 따른 소형선박의 저항성능 변화에 관한 연구)

  • Jun-Taek, Lim;Michael;Nam-Kyun, Im;Kwang-Cheol, Seo
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.29 no.6
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    • pp.672-680
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    • 2023
  • Weight is a critical factor in the ship design process given that it has a substantial impact on the hydrodynamic performance of ships. Typically, ships are optimally designed for specific conditions with a fixed draft and displacement. However, in reality, weight and draft can vary within a certain range owing to operational activities, such as fuel consumption, ballast adjustments, and loading conditions . Therefore, we investigated how resistance changes under three different loading conditions, namely overload, design-load, and lightship, for small craft, using both model experiments and numerical simulations. Additionally, we examined the sensitivity of weight changes to resistance to enhance the performance of ships, ultimately reducing power requirements in support of the International Maritime Organization's (IMO) goal of reducing CO2 emissions by 50% by 2050. We found that weight changes have a more significant impact at low Froude Numbers. Operating under overload conditions, which correspond to a 5% increase in draft and an 11.1% increase in displacement, can lead to a relatively substantial increase in total resistance, up to 15.97% and 14.31% in towing tests and CFD simulations, respectively.

miR-133a-3p and miR-145-5p co-promote goat hair follicle stem cell differentiation by regulating NANOG and SOX9 expression

  • Jian Wang;Xi Wu;Liuming Zhang;Qiang Wang;Xiaomei Sun;Dejun Ji;Yongjun Li
    • Animal Bioscience
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    • v.37 no.4
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    • pp.609-621
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    • 2024
  • Objective: Hair follicle stem cells (HFSCs) differentiation is a critical physiological progress in skin hair follicle (HF) formation. Goat HFSCs differentiation is one of the essential processes of superior-quality brush hair (SQBH) synthesis. However, knowledge regarding the functions and roles of miR-133a-3p and miR-145-5p in differentiated goat HFSCs is limited. Methods: To examine the significance of chi-miR-133a-3p and chi-miR-145-5p in differentiated HFSCs, overexpression and knockdown experiments of miR-133a-3p and miR-145-5p (Mimics and Inhibitors) separately or combined were performed. NANOG, SOX9, and stem cell differentiated markers (β-catenin, C-myc, Keratin 6 [KRT6]) expression levels were detected and analyzed by using real-time quantitative polymerase chain reaction, western blotting, and immunofluorescence assays in differentiated goat HFSCs. Results: miR-133a-3p and miR-145-5p inhibit NANOG (a gene recognized in keeping and maintaining the totipotency of embryonic stem cells) expression and promote SOX9 (an important stem cell transcription factor) expression in differentiated stem cells. Functional studies showed that miR-133a-3p and miR-145-5p individually or together overexpression can facilitate goat HFSCs differentiation, whereas suppressing miR-133a-3p and miR-145-5p or both inhibiting can inhibit goat HFSCs differentiation. Conclusion: These findings could more completely explain the modulatory function of miR-133a-3p and miR-145-5p in goat HFSCs growth, which also provide more understandings for further investigating goat hair follicle development.

Effects of Leisure Satisfaction on Health Promotion Behavior and Quality of Life of Participants In the Walking Program "Geod-Jyu" (걷기 프로그램 '걷쥬' 참여자의 여가만족이 건강증진행위 및 삶의 질에 미치는 영향)

  • Chi-Sub Hahn;Sung-Min Kim
    • Journal of The Korean Society of Integrative Medicine
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    • v.12 no.2
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    • pp.121-131
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    • 2024
  • Purpose : This study aimed to investigate the leisure satisfaction of participants in the Geod-Jyu walking program and to examine the relationship between health promotion behaviors and quality of life. In addition, we investigated the impact of leisure satisfaction with walking among program participants on health promotion behaviors and quality of life. By clarifying these relationships, we aimed to identify factors of leisure satisfaction with walking that could enhance participants' health promotion behaviors and quality of life. Methods : A survey was conducted among 301 participants enrolled in the Geod-Jyu walking program in Chungcheongnam-do, with 288 responses analyzed. Data analysis included Pearson's correlation, confirmatory factor analysis, and multiple regression analysis using SPSS 27.0. Result : Leisure satisfaction exhibited a positive correlation with health promotion behavior (r=.544, p<.01) and quality of life (r=.478, p<.01). Furthermore, health promotion behavior showed a positive correlation with quality of life (r=.636, p<.01). Leisure satisfaction positively influenced quality of life, with physiological satisfaction (β=.16, t=2.32) and relaxation satisfaction (β=.15, t=2.04) emerging as notable contributors. In addition, leisure satisfaction had a significant positive impact on health promotion behaviors, with psychological satisfaction (β=.24, t=3.09) and educational satisfaction (β=.20, t=3.09) playing key roles. Health promotion behavior had a positive impact on quality of life, with all sub variables exhibiting significant positive effects in the following order: self-actualization (β=.24, t=4.16), stress management (β=.22, t=3.97), exercise (β=.22, t=4.05), and health responsibility (β=.12, t=2.14). Conclusion : The findings indicate that factors related to physical and rest satisfaction, as well as health-promoting behaviors facilitated by walking, significantly affected the quality of life among Geod-Jyu participants. Thus, promoting leisure satisfaction and engaging in health-promoting activities through walking can enhance overall well-being. Encouraging participation in the Geod-Jyu walking program is critical for physical and psychological benefits, and for promoting healthier behaviors. Further development of Geod-Jyu is expected to enhance walking satisfaction, promote self-care skills for health management, and positively influence quality of life.

Effects of Season Differences on the Cecal Microbiome of Broiler at Conventional Farms and Welfare System Farms (계절에 따른 일반 농가와 복지 농가 육계의 맹장 내 미생물 균총에 미치는 영향)

  • Junsik Kim;Seol Hwa Park;Minji Kim;Seong Hoon Shim;Hwan Ku Kang;Jin Young Jeong
    • Korean Journal of Poultry Science
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    • v.51 no.2
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    • pp.73-82
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    • 2024
  • The gut microbiome of broilers is a critical factor in overall health and productivity. However, high summer temperatures and high stocking density (conventional farm condition) may cause stress to broilers, resulting in an imbalance in the gut microbiome. This study was conducted to compare the gut microbiome of broilers between spring and summer in welfare (Bosung, Jeollanam-do, South Korea) and conventional farms (Jangsu, Jeollabuk-do, South Korea). A total of 31 broilers were assigned to the following groups: conventional farm in spring (n = 8); conventional farm in summer (n = 8); welfare farm in spring (n = 7); welfare farm in summer (n = 8). Cecal digesta were collected from eight broilers from each farm, and microbiome analysis was performed using 16S rRNA gene sequencing. Beta diversity analysis indicated clear differences in cecal microbiome composition between spring and summerin both welfare and conventional farm. At the phylum level, analysis of conventional farm revealed a higher proportion of Bacteroidetes in spring than in summer. At the genus level, broilers exhibited a higher abundance of Bacteroides and Alistipesin spring compared to summer. In contrast, the difference in microbial flora composition observed in welfare farm was relatively small compared to conventional farm. In conclusion, the results of this study suggest that heat stress can negatively affect the caecum microbiome of broilers. However, improvements in the housing environment can mitigate the effects of heat stress.

A Study on Proposing the Harbor and Fishery Design Criteria for the Channel Width based on External Factors (외력에 따른 항로 폭 설정을 위한 항만 및 어항 설계기준에 관한 연구)

  • Dae-Han Lee;Yu-Min Kwon
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.30 no.2
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    • pp.184-193
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    • 2024
  • Harbor and Fishery Design Criteria defines that ship routing should be designed to ensure safe ship navigation and easy maneuvering. The design of the channel width is one of the critical conditions in routeing of ships. For the criteria of the channel width, qualitative evaluation criteria are used in South Korea, whereas quantitative evaluation criteria are used in other countries. Environmental factors, such as winds, tidal currents, and waves directly or indirectly affect ship resistance when sailing. Depending on their strength the environmental factors may pose a threat to the safe navigation of ships. Thus, vessel traffic control was regulated in South Korea according to the Beaufort scale. In order to design the channel width to reflect the environmental factors closely related to the safe navigation of ships, quantitative evaluation criteria reflected with environmental factors were presented in other countries, however, these external factors were not considered in South Korea. This study analyzed the relationship between the channel width actually used by ships and environmental factors by using the trajectories of ships entering and leaving Mokpo Port. This study also suggested the required channel width according to tidal currents and compared it with the channel width design criteria of other countries.

DEVELOPMENT OF STATEWIDE TRUCK TRAFFIC FORECASTING METHOD BY USING LIMITED O-D SURVEY DATA (한정된 O-D조사자료를 이용한 주 전체의 트럭교통예측방법 개발)

  • 박만배
    • Proceedings of the KOR-KST Conference
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    • 1995.02a
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    • pp.101-113
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    • 1995
  • The objective of this research is to test the feasibility of developing a statewide truck traffic forecasting methodology for Wisconsin by using Origin-Destination surveys, traffic counts, classification counts, and other data that are routinely collected by the Wisconsin Department of Transportation (WisDOT). Development of a feasible model will permit estimation of future truck traffic for every major link in the network. This will provide the basis for improved estimation of future pavement deterioration. Pavement damage rises exponentially as axle weight increases, and trucks are responsible for most of the traffic-induced damage to pavement. Consequently, forecasts of truck traffic are critical to pavement management systems. The pavement Management Decision Supporting System (PMDSS) prepared by WisDOT in May 1990 combines pavement inventory and performance data with a knowledge base consisting of rules for evaluation, problem identification and rehabilitation recommendation. Without a r.easonable truck traffic forecasting methodology, PMDSS is not able to project pavement performance trends in order to make assessment and recommendations in the future years. However, none of WisDOT's existing forecasting methodologies has been designed specifically for predicting truck movements on a statewide highway network. For this research, the Origin-Destination survey data avaiiable from WisDOT, including two stateline areas, one county, and five cities, are analyzed and the zone-to'||'&'||'not;zone truck trip tables are developed. The resulting Origin-Destination Trip Length Frequency (00 TLF) distributions by trip type are applied to the Gravity Model (GM) for comparison with comparable TLFs from the GM. The gravity model is calibrated to obtain friction factor curves for the three trip types, Internal-Internal (I-I), Internal-External (I-E), and External-External (E-E). ~oth "macro-scale" calibration and "micro-scale" calibration are performed. The comparison of the statewide GM TLF with the 00 TLF for the macro-scale calibration does not provide suitable results because the available 00 survey data do not represent an unbiased sample of statewide truck trips. For the "micro-scale" calibration, "partial" GM trip tables that correspond to the 00 survey trip tables are extracted from the full statewide GM trip table. These "partial" GM trip tables are then merged and a partial GM TLF is created. The GM friction factor curves are adjusted until the partial GM TLF matches the 00 TLF. Three friction factor curves, one for each trip type, resulting from the micro-scale calibration produce a reasonable GM truck trip model. A key methodological issue for GM. calibration involves the use of multiple friction factor curves versus a single friction factor curve for each trip type in order to estimate truck trips with reasonable accuracy. A single friction factor curve for each of the three trip types was found to reproduce the 00 TLFs from the calibration data base. Given the very limited trip generation data available for this research, additional refinement of the gravity model using multiple mction factor curves for each trip type was not warranted. In the traditional urban transportation planning studies, the zonal trip productions and attractions and region-wide OD TLFs are available. However, for this research, the information available for the development .of the GM model is limited to Ground Counts (GC) and a limited set ofOD TLFs. The GM is calibrated using the limited OD data, but the OD data are not adequate to obtain good estimates of truck trip productions and attractions .. Consequently, zonal productions and attractions are estimated using zonal population as a first approximation. Then, Selected Link based (SELINK) analyses are used to adjust the productions and attractions and possibly recalibrate the GM. The SELINK adjustment process involves identifying the origins and destinations of all truck trips that are assigned to a specified "selected link" as the result of a standard traffic assignment. A link adjustment factor is computed as the ratio of the actual volume for the link (ground count) to the total assigned volume. This link adjustment factor is then applied to all of the origin and destination zones of the trips using that "selected link". Selected link based analyses are conducted by using both 16 selected links and 32 selected links. The result of SELINK analysis by u~ing 32 selected links provides the least %RMSE in the screenline volume analysis. In addition, the stability of the GM truck estimating model is preserved by using 32 selected links with three SELINK adjustments, that is, the GM remains calibrated despite substantial changes in the input productions and attractions. The coverage of zones provided by 32 selected links is satisfactory. Increasing the number of repetitions beyond four is not reasonable because the stability of GM model in reproducing the OD TLF reaches its limits. The total volume of truck traffic captured by 32 selected links is 107% of total trip productions. But more importantly, ~ELINK adjustment factors for all of the zones can be computed. Evaluation of the travel demand model resulting from the SELINK adjustments is conducted by using screenline volume analysis, functional class and route specific volume analysis, area specific volume analysis, production and attraction analysis, and Vehicle Miles of Travel (VMT) analysis. Screenline volume analysis by using four screenlines with 28 check points are used for evaluation of the adequacy of the overall model. The total trucks crossing the screenlines are compared to the ground count totals. L V/GC ratios of 0.958 by using 32 selected links and 1.001 by using 16 selected links are obtained. The %RM:SE for the four screenlines is inversely proportional to the average ground count totals by screenline .. The magnitude of %RM:SE for the four screenlines resulting from the fourth and last GM run by using 32 and 16 selected links is 22% and 31 % respectively. These results are similar to the overall %RMSE achieved for the 32 and 16 selected links themselves of 19% and 33% respectively. This implies that the SELINICanalysis results are reasonable for all sections of the state.Functional class and route specific volume analysis is possible by using the available 154 classification count check points. The truck traffic crossing the Interstate highways (ISH) with 37 check points, the US highways (USH) with 50 check points, and the State highways (STH) with 67 check points is compared to the actual ground count totals. The magnitude of the overall link volume to ground count ratio by route does not provide any specific pattern of over or underestimate. However, the %R11SE for the ISH shows the least value while that for the STH shows the largest value. This pattern is consistent with the screenline analysis and the overall relationship between %RMSE and ground count volume groups. Area specific volume analysis provides another broad statewide measure of the performance of the overall model. The truck traffic in the North area with 26 check points, the West area with 36 check points, the East area with 29 check points, and the South area with 64 check points are compared to the actual ground count totals. The four areas show similar results. No specific patterns in the L V/GC ratio by area are found. In addition, the %RMSE is computed for each of the four areas. The %RMSEs for the North, West, East, and South areas are 92%, 49%, 27%, and 35% respectively, whereas, the average ground counts are 481, 1383, 1532, and 3154 respectively. As for the screenline and volume range analyses, the %RMSE is inversely related to average link volume. 'The SELINK adjustments of productions and attractions resulted in a very substantial reduction in the total in-state zonal productions and attractions. The initial in-state zonal trip generation model can now be revised with a new trip production's trip rate (total adjusted productions/total population) and a new trip attraction's trip rate. Revised zonal production and attraction adjustment factors can then be developed that only reflect the impact of the SELINK adjustments that cause mcreases or , decreases from the revised zonal estimate of productions and attractions. Analysis of the revised production adjustment factors is conducted by plotting the factors on the state map. The east area of the state including the counties of Brown, Outagamie, Shawano, Wmnebago, Fond du Lac, Marathon shows comparatively large values of the revised adjustment factors. Overall, both small and large values of the revised adjustment factors are scattered around Wisconsin. This suggests that more independent variables beyond just 226; population are needed for the development of the heavy truck trip generation model. More independent variables including zonal employment data (office employees and manufacturing employees) by industry type, zonal private trucks 226; owned and zonal income data which are not available currently should be considered. A plot of frequency distribution of the in-state zones as a function of the revised production and attraction adjustment factors shows the overall " adjustment resulting from the SELINK analysis process. Overall, the revised SELINK adjustments show that the productions for many zones are reduced by, a factor of 0.5 to 0.8 while the productions for ~ relatively few zones are increased by factors from 1.1 to 4 with most of the factors in the 3.0 range. No obvious explanation for the frequency distribution could be found. The revised SELINK adjustments overall appear to be reasonable. The heavy truck VMT analysis is conducted by comparing the 1990 heavy truck VMT that is forecasted by the GM truck forecasting model, 2.975 billions, with the WisDOT computed data. This gives an estimate that is 18.3% less than the WisDOT computation of 3.642 billions of VMT. The WisDOT estimates are based on the sampling the link volumes for USH, 8TH, and CTH. This implies potential error in sampling the average link volume. The WisDOT estimate of heavy truck VMT cannot be tabulated by the three trip types, I-I, I-E ('||'&'||'pound;-I), and E-E. In contrast, the GM forecasting model shows that the proportion ofE-E VMT out of total VMT is 21.24%. In addition, tabulation of heavy truck VMT by route functional class shows that the proportion of truck traffic traversing the freeways and expressways is 76.5%. Only 14.1% of total freeway truck traffic is I-I trips, while 80% of total collector truck traffic is I-I trips. This implies that freeways are traversed mainly by I-E and E-E truck traffic while collectors are used mainly by I-I truck traffic. Other tabulations such as average heavy truck speed by trip type, average travel distance by trip type and the VMT distribution by trip type, route functional class and travel speed are useful information for highway planners to understand the characteristics of statewide heavy truck trip patternS. Heavy truck volumes for the target year 2010 are forecasted by using the GM truck forecasting model. Four scenarios are used. Fo~ better forecasting, ground count- based segment adjustment factors are developed and applied. ISH 90 '||'&'||' 94 and USH 41 are used as example routes. The forecasting results by using the ground count-based segment adjustment factors are satisfactory for long range planning purposes, but additional ground counts would be useful for USH 41. Sensitivity analysis provides estimates of the impacts of the alternative growth rates including information about changes in the trip types using key routes. The network'||'&'||'not;based GMcan easily model scenarios with different rates of growth in rural versus . . urban areas, small versus large cities, and in-state zones versus external stations. cities, and in-state zones versus external stations.

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The Effect of Nitric Oxide Donor or Nitric Oxide Synthase Inhibitor on Oxidant Injury to Cultured Rat Lung Microvascular Endothelial Cells (산화질소 공여물과 산화질소 합성효소 길항제가 백서 폐미세혈관 내피세포 산화제 손상에 미치는 영향)

  • Chang, Joon;Michael, John R.;Kim, Se-Kyu;Kim, Sung-Kyu;Lee, Won-Young;Kang, Kyung-Ho;Yoo, Se-Hwa;Chae, Yang-Seok
    • Tuberculosis and Respiratory Diseases
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    • v.45 no.6
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    • pp.1265-1276
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    • 1998
  • Background : Nitric oxide(NO) is an endogenously produced free radical that plays an important role in regulating vascular tone, inhibition of platelet aggregation and white blood cell adhesion to endothelial cells, and host defense against infection. The highly reactive nature of NO with oxygen radicals suggests that it may either promote or reduce oxidant-induced cell injury in several biological pathways. Oxidant injury and interactions between pulmonary vascular endothelium and leukocytes are important in the pathogenesis of acute lung injury, including acute respiratory distress syndrome(ARDS). In ARDS, therapeutic administration of NO is a clinical condition providing exogenous NO in oxidant-induced endothelial injury. The role of exogenous NO from NO donor or the suppression of endogenous NO production was evaluated in oxidant-induced endothelial injury. Method : The oxidant injury in cultured rat lung microvascular endothelial cells(RLMVC) was induced by hydrogen peroxide generated from glucose oxidase(GO). Cell injury was evaluated by $^{51}$chromium($^{51}Cr$) release technique. NO donor, such as S-nitroso-N-acetylpenicillamine(SNAP) or sodium nitroprusside(SNP), was added to the endothelial cells as a source of exogenous NO. Endogenous production of NO was suppressed with N-monomethyl-L-arginine(L-NMMA) which is an NO synthase inhibitor. L-NMMA was also used in increased endogenous NO production induced by combined stimulation with interferon-$\gamma$(INF-$\gamma$), tumor necrosis factor-$\alpha$(TNF-$\alpha$), and lipopolysaccharide(LPS). NO generation from NO donor or from the endothelial cells was evaluated by measuring nitrite concentration. Result : $^{51}Cr$ release was $8.7{\pm}0.5%$ in GO 5 mU/ml, $14.4{\pm}2.9%$ in GO 10 mU/ml, $32.3{\pm}2.9%$ in GO 15 mU/ml, $55.5{\pm}0.3%$ in GO 20 mU/ml and $67.8{\pm}0.9%$ in GO 30 mU/ml ; it was significantly increased in GO 15 mU/ml or higher concentrations when compared with $9.6{\pm}0.7%$ in control(p < 0.05; n=6). L-NMMA(0.5 mM) did not affect the $^{51}Cr$ release by GO. Nitrite concentration was increased to $3.9{\pm}0.3\;{\mu}M$ in culture media of RLMVC treated with INF-$\gamma$ (500 U/ml), TNF-$\alpha$(150 U/ml) and LPS($1\;{\mu}g/ml$) for 24 hours ; it was significantly suppressed by the addition of L-NMMA. The presence of L-NMMA did not affect $^{51}Cr$ release induced by GO in RLMVC pretreated with INF-$\gamma$, TNF-$\alpha$ and LPS. The increase of $^{51}Cr$ release with GO(20 mU/ml) was prevented completely by adding 100 ${\mu}M$ SNAP. But the add of SNP, potassium ferrocyanate or potassium ferricyanate did not protect the oxidant injury. Nitrite accumulation was $23{\pm}1.0\;{\mu}M$ from 100 ${\mu}M$ SNAP at 4 hours in phenol red free Hanks' balanced salt solution. But nitrite was not detectable from SNP upto 1 mM The presence of SNAP did not affect the time dependent generation of hydrogen peroxide by GO in phenol red free Hanks' balanced salt solution. Conclusion : Hydrogen peroxide generated by GO causes oxidant injury in RLMVC. Exogenous NO from NO donor prevents oxidant injury, and the protective effect may be related to the ability to release NO. These results suggest that the exogenous NO may be protective on oxidant injury to the endothelium.

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