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Blood Vessel Regeneration using Human Umbilical Cord-derived Endothelial Progenitor Cells in Cyclophosphamide-treated Immune-deficient Mice

  • Kwon, Soon-Keun;Ko, Yu-Jin;Cho, Tae-Jun;Park, Eu-Gene;Kang, Byung-Chul;Lee, Gene;Cho, Jae-Jin
    • International Journal of Oral Biology
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    • v.36 no.3
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    • pp.117-122
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    • 2011
  • Endothelial cells are a vital constituent of most mammalian organs and are required to maintain the integrity of these tissues. These cells also play a major role in angiogenesis, inflammatory reactions, and in the regulation of thrombosis. Angiogenesis facilitates pulp formation and produces the vessels which are essential for the maintenance of tooth homeostasis. These vessels can also be used in bone and tissue regeneration, and in surgical procedures to place implants or to remove cancerous tissue. Furthermore, endothelial cell regeneration is the most critical component of the tooth generation process. The aim of the present study was to stimulate endothelial regeneration at a site of acute cyclophosphamide (CP)-induced endothelial injury by treatment with human umbilical cord-derived endothelial/mesenchymal stem cells (hEPCs). We randomly assigned 16 to 20-week-old female NOD/SCID mice into three separate groups, a hEPC ($1{\times}10^5$ cells) transplanted, 300mg/kg CP treated and saline (control) group. The mice were sacrificed on days 5 and 10 and blood was collected via the abdominal aorta for analysis. The alanine transaminase (ALT), aspartate aminotransferase (AST), serum alkaline phosphatase (s-ALP), and albumin (ALB) levels were then evaluated. Tissue sections from the livers and kidneys were stained with hematoxylin and eosin (HE) for microscopic analysis and were subjected to immunohistochemistry to evaluate any changes in the endothelial layer. CP treatment caused a weight reduction after one day. The kidney/body weight ratio increased in the hEPC treated animals compared with the CP only group at 10 days. Moreover, hEPC treatment resulted in reduced s-ALP, AST, ALT levels compared with the CP only group at 10 days. The CP only animals further showed endothelial injuries at five days which were recovered by hEPC treatment at 10 days. The number of CD31-positive cells was increased by hEPC treatment at both 5 and 10 days. In conclusion, the CP-induced disruption of endothelial cells is recovered by hEPC treatment, indicating that hEPC transplantation has potential benefits in the treatment of endothelial damage.

Effect of Configuration of Shaft and Helix Plate on Bearing Capacity of Moderate-size Helical Pile : I. Test-bed Construction and Field Loading Test (중소구경 헬리컬 파일의 축과 원판의 형상이 지지력에 미치는 영향 평가 : I. 시험시공과 현장재하시험)

  • Lee, Jongwon;Lee, Dongseop;Kim, Hyung-Nam;Choi, Hangseok
    • Journal of the Korean Geosynthetics Society
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    • v.13 no.2
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    • pp.31-39
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    • 2014
  • The helical pile is a manufactured steel pile consisting of one or more helix-shaped bearing plates affixed to a central shaft. This pile is installed by rotating the shaft into the ground to support structural loads. The advantages of helical piles are no need for boring or grout process, and ability to install with relatively light devices. The bearing capacity of the helical pile is exerted by integrating the bearing capacity of each helix plate attached to the steel shaft. In this paper, to estimate the bearing capacity of moderate-size helical piles, 6 types of helical piles were constructed with different shaft diameter, plate configuration and the penetration depth. A series of field loading tests was performed to evaluate the effect of helical pile configuration on the bearing capacity of helical pile, constructed in two different shaft diameters (i.e. 73 mm and 114 mm). In the same way, the diameter of bearing plate was also changed from 400mm to 250mm with one or three plates. As well, the penetration depth was varied from 3m to 6m to analyze the relation between the penetration depth and the bearing capacity. As a result, not only the increase of the shaft diameter, but also the number or diameter of helix bearing plates enhances the bearing capacity. Especially the configuration of the helix plate is more critical than the shaft diameter.

A Critical Study on the Landform Recognition of Daegu City as an Intermontane Basin (대구 산간분지 지형 인식에 대한 비판적 고찰)

  • Lee, Jaeha
    • Journal of the Korean Geographical Society
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    • v.51 no.3
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    • pp.327-344
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    • 2016
  • We may have an incorrect view of Korea and the world by misunderstanding them with a number of geographic misconceptions. Such misconceptions constructed socially tend to perpetuate through reproducing and learning repeatedly from one generation to the next. 'Daegu city is in the intermontane basin.' It is also identified that this geographic misconception had constructed (made) by two Japanese geographers (Tamura, 1933; Tada, 1940) in the Japanese colonial period, and have been reproduced and diffused by many Korean geographers (professors and teachers) as well as journalists in the post-colonial days. In terms of the definition of an intermontane basin in the Encyclopedia of Geomorphology published by the International Association of Geomorphologists, Daegu seems not to be a basin city but to be a plain city, since the central plain of Daegu is surrounded by higher terrain like mountains and hills only on the north and south directions of all sides, and also it is well developed thanks to its location where the downstream of the Geumhogang river flows from east to west. This paper hopes that the landform recognition as 'Daegu intermontane basin city' should be corrected as soon as possible, and also many geographic misconceptions will be studied actively for an accurate understanding of Korea and the world.

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The Other 90%, Warm Technology, and the Best Solution: A Critique of the Appropriate Technology Movement in South Korea (소외된 90%, 따뜻한 기술, 최고의 솔루션: 한국 적정기술 운동의 문제의식 비판)

  • Jeon, Chihyung
    • Journal of Science and Technology Studies
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    • v.14 no.2
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    • pp.127-164
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    • 2014
  • This essay examines the motivations, goals, and assumptions of those who are participating in the appropriate technology movement in contemporary South Korea. In addition to analyzing books, articles, presentation materials, and media reports written by or about the "appropriate technologists," I have interviewed a few of them to hear their thoughts on appropriate technologies as well as inappropriate technologies. They choose to work on appropriate technology out of their good will for people in need, their Christian faith, and their pride in Korea's rapid and successful development. As a result, the appropriate technology movement in Korea does not engage in a critical evaluation of current sociotechnical systems, but instead takes an ethical and paternalistic stance in accepting and improving the given situation. Its apolitical character is effective in drawing a large number of participants, but it also limits their imagination and the movement's potential influence. Moreover, the movement's focus on "the other 90%" leads the participants to frame appropriate technology as something for "them" or "locals" rather than as something for "all of us." This essay concludes by suggesting that the appropriate technology movement in Korea should expand its scope from an ethical pursuit of technology to a political engagement with technology.

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Comparative Studies of Perceiving Korean Monosyllabic Digit Words under Different Speech Compression Schemes (음성압축 방식의 변화가 한국어 단음절 숫자 인지기능에 미치는 영향의 비교 연구)

  • Lim, Dukhwan;Won, Yookyung
    • The Journal of the Acoustical Society of Korea
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    • v.32 no.3
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    • pp.262-268
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    • 2013
  • The performances of speech compression schemes appeared to be dependent on the response profiles to compressed stimuli and the features of individual languages to some extent. Although these response profiles were critical in comparing various compression outcomes, the related data were limited in number for Korean monosyllabic words. From the previous study, data from PNT (Preserving No Trait) compression was selected as a base set for comparison. In this study, the outcomes from PPT (Preserving Pitch Trait) and PTT (Preserving Time Trait) were analyzed under the same condition. Then, the properties of these three widely used representative compression schemes were quantitatively compared in normal hearing adults (N=20) for controlled Korean quintet digit sets (0 through 9). Results showed that PPT compression scheme exhibited the best perceptual performances for the Korean quintet digit sets in the final outcomes (PPT>PTT>PNT). The compression ratios of 50% performances were estimated as about 20%, 42%, and 44% for PPT, PTT, and PNT, respectively. The data indicated the influences of the salient psychoacoustic features of the three representative compressions on perceiving Korean monosyllabic digit words. This controlled procedure with monosyllabic quintet sets can evaluate efficiency and effectiveness of other compression schemes and may also contribute to diagnosing auditory processing disorders and fitting special hearing aids with compression issues.

Chest-wall Surface Dose During Post-mastectomy Radiation Therapy, with and without Nonmagnetic Bolus: A Phantom Study

  • Choi, Cheon Woong;Hong, Joo Wan;Park, Cheol Soo;Ahn, Jae Ouk
    • Journal of Magnetics
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    • v.21 no.2
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    • pp.293-297
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    • 2016
  • For mastectomy patients, sufficient doses of radiation should be delivered to the surface of the chest wall to prevent recurrence. A bolus is used to increase the surface dose on the chest wall, whereby the surface dose is confirmed with the use of a virtual bolus during the computerized treatment-planning process. The purpose of this study is an examination of the difference between the dose of the computerized treatment plan and the dose that is measured on the bolus. Part of the left breast of an Anderson Rando phantom was removed, followed by the attainment of computed tomography (CT) images that were used as the basis for computerized treatment plans that were established with no bolus, a 3 mm-thick bolus, a 5 mm-thick bolus, and a 10 mm-thick bolus. For the computerized treatment plan, a prescribed dose regimen was dispensed daily and planning target volume (PTV) coverage was applied according to the RTOG 1304 guidelines. Using each of the established computerized treatment plans, chest-wall doses of 5 points were measured; this chest-wall dose was used as the standard for the analysis of this study, while the level of significance was set at P < 0.05. The measurement of the chest-wall dose with no bolus is 1.6 % to 10.3 % higher, and the differences of the minimum average and the maximum average of the five measurement points are -13.8 and -1.9, respectively (P < 0.05); however, when the bolus was used, the dosage was measured as 3.7 % to 9.2 % lower, and the differences of the minimum average and the maximum average are 7.4 and 9.0, -1.2 and 17.4, and 8.1 and 19.8 for 3 mm, 5 mm, and 10 mm, respectively (P < 0.05). As the thickness of the bolus is increased, the differences of the average surface dose are further increased. There are a variety of factors that affect the surface dose on the chest wall during post-mastectomy radiation therapy, for which verification is required; in particular, a consideration of the appropriate thickness and the number of uses when a bolus is used, and which has the greatest effect on the surface dose on the chest wall, is considered necessary.

Microfiltration Characteristics for Emulsified Oil in Water (에멀젼형 오일 수용액에 관한 정밀여과 특성)

  • ;;;Fane, Anthony G.
    • Membrane Journal
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    • v.8 no.4
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    • pp.203-209
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    • 1998
  • The cutting oil emulsion microfiltration was carried out on dead-end call and crossflow systems equipped with 0.22 $\mu$m GVHP Millipore and 0.2 m stainless steel Mott microfiltration membranes, respectivdy. The oil drop size in the emulsion was varied from 0.07 to 0.22 $\mu$m. Cake filtration(CFM) and standard pore blocking models(SPBM) were applied to predict the permeation flux. The permeation fluxes of 0.01 vol% oil emulsion followed CFM for dead-end system very well under the condition of 400 rpm and below 100 kPa. The SPBM was, however, suitable for the permeation flux at 400 rpm and above 150 kPa. The oil layer on the membrane surface was destroyed and reproduced repeatedly as operating pressure was suddenly changed from 60 to 200 kPa, and then returned to 60 kPa. Also, we estimated the critical entry pressure(CEP) which is changed from CFM to SPBM, and CEP for dead-end system was around 100 kPa. The CEP increased from around 100 to 150 kPa for the crossflow system as the oil concentration increased from 0.01 to 0.03 vol% when Reynolds number was 7080.

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The Post-IMF firm strategy and the corporate restructuring in the heavy & chemical industrial district: the case of Ulsan, Korea (울산 중화학공업의 재구조화 특성 - IMF 체제 이후의 기업전략을 중심으로 -)

  • Park, Yang-Choon
    • Journal of the Korean association of regional geographers
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    • v.7 no.2
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    • pp.17-34
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    • 2001
  • This paper is to analyze how firms in a large firm-led industrial city have carried out the restructuring in the face of radical shifts, with focus on the strategy and the restructuring of firms in Ulsan, a typical industrial district in Korea that is specialized in heavy & chemical industry. It has been well known that the local economy has been led by a small number of large firms, including affiliates of chaebol, and its industrial structure has also been characterised as a clear dichotomy between large firms as a customer and small and medium-size firms as a supplier, which can be called not horizontal but vertical relations. It can identify some tendencies, however, that local companies have been rather dynamically changing in response to increasingly turbulent environment since the Asian crisis. Some are radical, but some incremental. These can be summarized in four distinctive but interlinked ways. First, more than half of local companies surveyed have attempted to change their production systems, mainly from the fordist mass production towards the flexible mass production, seeking both economies of scale and scope. Second, local firms have vigorously continued to reorganize the boundary of the production and the organization, by specializing products and focusing on the core competence in order to save costs and cope with radically changing customer demands in a flexible way. Third, there have been various strategies for the organizational innovation such as the introduction of team organization, the boundary blurring between the managerial and production workers and the intra-firm spin-offs, so as to improve managerial efficiency and competence in the use of internal labour market. Finally, they have tried to be more sensitive to the market and customers. These tendencies seem to be increasingly critical to sustain their competitiveness. To do so, they tend to focus increasingly not only on the competing via the product quality rather than through price, but also to seek to diversify the market and customer firms beyond national boundary.

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Investigation on Influencing Environmental Factors on Health Status of Korean Septuagenarians Dwelling in Longevity Region in Jeonla Province (전라도 농촌장수지역 거주 70대 노인의 건강상태에 영향을 미치는 환경적 요인에 대한 탐색 연구)

  • Kwak, Chung Shil;Yon, Miyong;Lee, Mee Sook;Oh, Se In;Park, Sang Chul
    • Korean Journal of Community Nutrition
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    • v.19 no.2
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    • pp.142-162
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    • 2014
  • Objectives: To evaluate the critical environmental factors on healthy-aging of Korean people, we investigated the significant factors influencing health status of septuagenarians living in rural area of Jeonla province, known to be one of the representative longevity regions in Korea. Methods: We divided subjects into healthy group (36M/25F) or poor-health group (26M/73F) based on self-reported health status, body mass index, a number of prescription, and blood test data. General characteristics, physical measurements, lifestyle, dietary behavior and nutrient intake, physical health and mental health data were statistically compared between the two groups. Results: Average age was not different between healthy group and poor-health group in men and women, respectively. In men, significantly favorable factors to health were observed to be higher education, regular exercise, higher grip strength and walking function, body mass index (${\geq}18.5kg/m^2$), moderate frequency of drinking and eating-out, non-smoking, normal red blood cell (RBC) count, higher serum dehydroepiandrosterone-sulfate (DHEAS) level, good digestive function and appetite, normal hearing function, regular meals, adequate vegetable and fruit intake, diverse food intake, adequate energy and nutrients (protein, vitamin $B_1$, $B_6$, C and E, folate, niacin, P, Zn and K) intake, higher mini-nutrient status assessment (MNA) score and low level of depression. On the other hand, in women, those were literacy, living arrangement, moderate frequency of drinking, healthy teeth, higher grip strength and walking function, bone mineral density, normal RBC and white blood cell (WBC) count, higher DHEAS concentration, higher MNA score, normal cognition and memory function, having snack and adequate fruit intake. Conclusions: These results could be useful to plan effective strategies to increase health-life expectancy of Korean old people living in rural areas.

Advanced Victim Cache with Processor Reuse Information (프로세서의 재사용 정보를 이용하는 개선된 고성능 희생 캐쉬)

  • Kwak Jong Wook;Lee Hyunbae;Jhang Seong Tae;Jhon Chu Shik
    • Journal of KIISE:Computer Systems and Theory
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    • v.31 no.12
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    • pp.704-715
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    • 2004
  • Recently, a single or multi processor system uses the hierarchical memory structure to reduce the time gap between processor clock rate and memory access time. A cache memory system includes especially two or three levels of caches to reduce this time gap. Moreover, one of the most important things In the hierarchical memory system is the hit rate in level 1 cache, because level 1 cache interfaces directly with the processor. Therefore, the high hit rate in level 1 cache is critical for system performance. A victim cache, another high level cache, is also important to assist level 1 cache by reducing the conflict miss in high level cache. In this paper, we propose the advanced high level cache management scheme based on the processor reuse information. This technique is a kind of cache replacement policy which uses the frequency of processor's memory accesses and makes the higher frequency address of the cache location reside longer in cache than the lower one. With this scheme, we simulate our policy using Augmint, the event-driven simulator, and analyze the simulation results. The simulation results show that the modified processor reuse information scheme(LIVMR) outperforms the level 1 with the simple victim cache(LIV), 6.7% in maximum and 0.5% in average, and performance benefits become larger as the number of processors increases.