• Molecules and Cells
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• v.22 no.1
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• pp.97-103
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• 2006

Phosphoinositides are critical regulators of ion channel and transporter activity. There are multiple isomers of biologically active phosphoinositides in the plasma membrane and the different lipid species are non-randomly distributed. However, the mechanism by which cells impose selectivity and directionality on lipid movements and so generate a non-random lipid distribution remains unclear. In the present study we investigated which structural elements of phosphoinositides are responsible for their subcellular location and movement. We incubated phosphatidylinositol (PI), phosphatidylinositol 4-monophosphate (PI(4)P) and phosphatidylinositol 4,5-bisphosphate ($PI(4,5)P_2$) with short or long acyl chains in CHO and HEK cells. We show that phosphate number and acyl chain length determine cellular location and translocation movement. In CHO cells, $PI(4,5)P_2$ with a long acyl chain was released into the cytosol easily because of a low partition coefficient whereas long chain PI was released more slowly because of a high partition coefficient. In HEK cells, the cellular location and translocation movement of PI were similar to those of PI in CHO cells, whereas those of $PI(4,5)P_2$ were different; some mechanism restricted the translocation movement of $PI(4,5)P_2$, and this is in good agreement with the extremely low lateral diffusion of $PI(4,5)P_2$. In contrast to the dependence on the number of phosphates of the phospholipid head group of long acyl chain analogs, short acyl chain phospholipids easily undergo translocation movement regardless of cell type and number of phosphates in the lipid headgroup.

• Proceedings of the Korean Institute Of Construction Engineering and Management
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• 2007.11a
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• pp.756-761
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• 2007

The construction Supply Chain Management(SCM) plays a critical role to proceed construction projects successfully. It is necessary to develop a system to support better communication among various actors and to supply construction materials in the right place on time with unstable situations on site according to the planned construction schedule for effective Supply Chain Management. Therefore, the objective of this study is to propose an automated and intelligent process that can be used to utilize ubiquitous technologies in construction supply chain management. This process consists of various equipments that can collect, store, calculate and communicate data among themselves. This study deals with a conceptual idea in order to improve process and equipment in the supply chain management process.

• Advanced Industrial SCIence
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• v.2 no.2
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• pp.1-8
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• 2023

The goal of the study is to describe blockchain technology as it relates to enhancing supply chains in the retail sector in order to achieve sustainability. This study offers a critical analysis of the possible applications of blockchain technology and smart contracts to supply chain management. This paper explains how Blockchain technology may be used by customers and merchants in a variety of retail business operations to great advantage. By adopting a modified version of the UTUAT model, this study validates the possibility of using blockchain for supply chain management in the retail industry. The study found a significant and positive correlation between behavioral intention and acceptance toward employing block networks in supply chain management in the retail business. The behavior intention (BI) to adopt blockchain technology is significantly influenced by performance expectations, effect expectations, subjective standards, and enabling variables. The performance and effort expectations have a considerable impact on the BI to adopt blockchain in supply chain management.

• Tuberculosis and Respiratory Diseases
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• v.69 no.3
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• pp.207-211
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• 2010

Novel influenza A (H1N1) virus is a common pathogen of febrile respiratory infection recently. Here, we report the case of a 63-year-old male patient who presented with 3 days' ongoing cough and fever. He was diagnosed with novel influenza A (H1N1) pneumonia by real-time reverse-transcriptase-polymerase-chain-reaction (rRT-PCR). During treatment for novel influenza A (H1N1), his symptoms and radiologic findings improved initially, but multiple lung nodules developed subsequently and found on chest x-ray (on the 5th hospital day). Mycobacterium abscessus was isolated repeatedly from sputum and bronchoalveolar lavage fluid. To our knowledge, this is the first reported case of Mycobacterium abscessus lung disease in a patient with H1N1 influenza pneumonia.

• Journal of Digital Convergence
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• v.10 no.3
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• pp.51-58
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• 2012

The goal of this paper is to set up the rank list of the critical success factors according to its weights. To get the empirical data for this research, the Internet-based questionnaires were performed. This research is carried out in two steps. At first step, the critical success factors(CSF) are crystallized through factor analysis methods. To find out the weights and ranks of critical success factors in second step, the analytical hierarchical process(AHP) method were performed. The order list of all CSFs will be determined according to their priorities. This order list shows which factors have the most useful influence on the performance and success of SCM. This result could play the role as milestone for the effective management of Supply Chain Management System in korean manufacturing company.

• Journal of Microbiology and Biotechnology
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• v.33 no.6
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• pp.707-714
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• 2023

Plant-derived insecticide-neonicotinoid insecticides (NIs) played a crucial role in the development of agriculture and food industry in recent years. Nevertheless, synthesis of these nitrogen-containing heterocyclic compounds with an effective and greener routing remains challenging especially to the notion raise of "green chemistry" and "atom economy". While bio-catalyzed methods mediated by nicotinate dehydrogenase (NDHase) then provide an alternative. The current review mainly focuses on the introduction of sources, components, structure, catalytic mechanism and applications of NDHase. Specifically, NDHase is known as nicotinic acid hydroxylase and the sources principally derived from phylum Proteobacteria. In addition, NDHase requires the participation of the electron respiratory chain system on the cell membrane. And the most important components of the electron respiratory chain are hydrogen carrier, which is mainly composed of iron-sulfur proteins (Fe-S), flavin dehydrogenase (FAD), molybdenum binding protein and cytochromes. Heterologous expression studies were hampered by the plasmid and host with high efficiency and currently only Pseudomonas entomophila L48 as well as Comamonas testosterone was successfully utilized for the expression of NDHase. Furthermore, it is speculated that the conjugate and inductive effects of the substituent group at position 3 of the substrate pyridine ring exerts a critical role in the hydroxylation reactions at position 6 concerning about the substrate molecular recognition mechanism. Finally, applications of NDHase are addressed in terms of pesticide industry and wastewater treatment. On conclusion, this critical review would not only deepen our understanding of the theory about NDHase, but also provides the guideline for future investigation of NDHase.

• Tuberculosis and Respiratory Diseases
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• v.78 no.1
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• pp.1-7
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• 2015

Background: The incidence of tuberculosis (TB) in Korea is relatively high compared to the other Organisation for Economic Co-operation and Development (OECD) countries, with a prevalence of 71 per 100,000 in 2012, although the incidence is declining. Real-time polymerase chain reaction (PCR) has been introduced for the rapid diagnosis of TB. Recently, its advantage lies in higher sensitivity and specificity for the diagnosis of TB. This study evaluated the clinical accuracy of real-time PCR using respiratory specimens in a clinical setting. Methods: Real-time PCR assays using sputum specimens and/or bronchoscopic aspirates from 2,877 subjects were reviewed retrospectively; 2,859 subjects were enrolled. The diagnosis of TB was determined by positive microbiology, pathological findings of TB in the lung and pleura, or clinical suspicion of active TB following anti-TB medication for more than 6 months with a favorable response. Results: Sensitivity, specificity, and accuracy were 44%, 99%, and 86% from sputum, and 65%, 97%, and 87% from bronchoscopic aspirates, respectively. For overall respiratory specimens, sensitivity was 59%, specificity was 98%, and accuracy increased to 89%. Conclusion: Positivity in real-time PCR using any respiratory specimens suggests the possibility of active TB in clinically suspected cases, guiding to start anti-TB medication. Real-time PCR from selective bronchoscopic aspirates enhances the diagnostic yield much more when added to sputum examination.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.38 no.2
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• pp.303-310
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• 2018

One of most shocking headlines is a serious chain collision accident (CCA). The development of CCA has a temporal and spatial locality, and the information of the CCA is time-critical. Due to these characteristics of CCA, traffic accident information should be rapidly propagated to drivers in order to reduce chain collisions, right after the first accident occurs. Inter-vehicle communication (IVC) based on ad-hoc communication is one of promising alternatives for locally urgent information propagation. Despite this potential of IVC, research for the effects of IVC on the reduction of CCA has not been reported so far. Therefore, this study develops the parallel platform of microscopic vehicle and IVC communication simulators and then analyses the effects of IVC on the reduction of the second collision related to a series of vehicles. To demonstrate the potential of the IVC-based propagation of urgent traffic accident information for the reduction of CCA, the reduction of approaching-vehicle speed, the propagation speed of accident information, and then the reduction of CCA were analysed, respectively, according to scenarios of combination of market rates and traffic volumes. The analysis results showed that CCA can be effectively reduced to 40~60% and 80~82% at the penetration rates of 10% and 50%, respectively.

• The Korean Journal of Physiology and Pharmacology
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• v.27 no.2
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• pp.131-141
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• 2023

Compelling evidence has demonstrated the critical role of circular RNAs (circRNAs) during lung adenocarcinoma (LUAD) progression. Herein, we explored a novel circRNA, circ_0129047, and detailed its mechanism of action. The expression of circ 0129047, microRNA-665 (miR-665), and protein tyrosine phosphatase receptor type B (PTPRB) in LUAD tissues and cells was determined using reverse transcription quantitative polymerase chain reaction and Western blotting. Cell Counting Kit8 and colony formation assays were conducted to detect LUAD cell proliferation, and western blotting was performed to quantify apoptosis-related proteins (Bcl2 and Bax). Luciferase reporter and RNA immunoprecipitation assays were used to validate the predicted interaction between miR-665 and circ_0129047 or PTPRB. A xenograft assay was used for the in vivo experiments. Circ_0129047 and PTPRB were downregulated in LUAD tissues and cells, whereas miR-665 expression was upregulated. Overexpression of circ_0129047 suppresses LUAD growth in vivo and in vitro. Circ_0129047 is the target of miR-665, and the miR-665 mimic ablated the antiproliferative and pro-apoptotic phenotypes of LUAD cells by circ_0129047 augmentation. MiR-665 targets the 3'UTR of PTPRB and downregulates PTPRB expression. PTPRB overexpression offsets the pro-proliferative potential of miR-665 in LUAD cells. Circ_0129047 sequestered miR-665 and upregulated PTPRB expression, thereby reducing LUAD progression, suggesting a promising approach for preventing LUAD.

• International Commerce and Information Review
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• v.13 no.3
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• pp.203-228
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• 2011

In one global industry after another, supply chains are reaching across continents farther and deeper than they have ever been. As a result, global companies are confronting new and more formidable supply chain risks which insurance policies alone cannot overcome. This is especially true in the Korean manufacturing industry, where the integration of Supply Chain Management and Risk Management has become critical among Korean export manufactures. One of the most effective ways to manage supply chain risks is to prevent them from occurrence. First, however, supply chain managers must have the knowledge to identify potential causes of supply chain risks. This paper attempts to contribute to such knowledge by examining the relationships between Risk Management, Business Partnership, and Business Performance among Korean export manufacturers. Our study analysed 105 cases in Korean supply chain management and then developed a structural equation model. From our model we found that Supply Chain Risk Management affects both Business Performance and Business Partnerships among Korean export manufacturers. Our model also revealed that the practice of Risk Management within the Supply Chain of Korean export manufacturing industry is impeded by certain constraints and limitations.