• The Journal of Korean Physical Therapy
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• v.13 no.3
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• pp.685-692
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• 2001

Study the variation of low frequency electrical stimulation on human metabolism which serum to test the increasement of creatine- kinase, myoglobin, lactate .and pyruvate. The results were as followed: 1) Creatine kinase increasement showed significant difference between 200 Hz stimulation group and control group, and also between 300Hz stimulation group and control group, but no significant increasement difference showed in 50 Hz stimulation group compared to control group. 2) Lactate increasement showed significant difference in 200 Hz stimulation group and 300 Hz stimulation group compared to control group, but no significant difference showed in 50 Hz stimulation group. 3) Pyruvate increasement showed significant difference in 50 Hz stimulation group and 200 Hz stimulation group compared to control group, but no significant increasement difference showed between 50 Hz stimulation group and control group. (P < 0.05) 4) Myoglobin increasement showed significant difference in 50Hz stimulation group and 200 Hz stimulation group compared to control group, and also in 200 Hz stimulation group and 300 Hz stimulation group compared to 50Hz stimulation group. ( P < 0.05)

• Annals of Clinical Neurophysiology
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• v.19 no.1
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• pp.40-45
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• 2017

Background: Telbivudine is a nucleoside analogue used for the treatment of chronic hepatitis B, but it often develops mitochondrial toxicity leading to symptomatic myopathy. In this study, three patients with telbivudine induced myopathy were enrolled in order to investigate the nature and pathogenesis of mitochondrial toxicity caused by long-term use of telbivudine. Methods: Clinical features, laboratory findings, muscle pathology, and quantitation of mitochondrial DNA were studied in three patients. Results: Patients presented with progressive muscle weakness with high serum creatine kinase levels. Light microscopic findings of muscle pathology showed ragged red fibers that reacted strongly with succinate dehydrogenase stain, but negative for cytochrome c oxidase activities. Electron microscopy revealed abnormal mitochondrial accumulation with rod shaped inclusions. The quantitative peroxidase chain reaction showed a depletion of mitochondrial DNA in skeletal muscle of the patients. Conclusions: Nucleoside analogues including telbivudine are potent inhibitors of viral DNA polymerases. However, they are not specific for viral DNA and can disturb mitochondrial replication at the same time. All nucleotide analogues should be used with close clinical observation in order to avoid development of mitochondrial myopathy.

• Toxicological Research
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• v.35 no.3
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• pp.233-239
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• 2019

Nerium oleander (N. oleander) is a well-known poisonous shrub that is frequently grown in gardens and public areas and contains numerous toxic compounds. The major toxic components are the cardiac glycosides oleandrin and neriin. The aim of our study was to evaluate the toxic effects of an ethanolic N. oleander leaf extract on haematological, cardiac, inflammatory, and serum biochemical parameters, as well as histopathological changes in the heart. N. oleander extract was orally administered for 14 and 30 consecutive days at doses of 100 and 200 mg of dried extract/kg of body weight in 0.5 mL of saline. The results showed significant increases in mean corpuscular volume, white blood cell counts, platelet counts, interleukins (IL-1 and IL-6), tumour necrosis factor alpha, C reactive protein, alanine aminotransferase, lactate dehydrogenase, creatine kinase and creatine kinase MB, especially at high doses. Marked pathological changes were perceived in the heart tissue. Thus, it can be concluded that exposure to N. oleander leaf extract adversely affects the heart and liver.

• Korean Journal of Exercise Nutrition
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• v.23 no.2
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• pp.1-6
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• 2019

[Purpose] Strenuous exercise often induces skeletal muscle damage, which results in impaired performance. Sphingolipid metabolism contributes to various cellular processes, including apoptosis, stress response, and inflammation. However, the relationship between exercise-induced muscle damage and ceramide (a key component of sphingolipid metabolism), is rarely studied. The present study aimed to explore the regulatory role of sphingolipid metabolism in exercise-induced muscle damage. [Methods] Mice were subjected to strenuous exercise by treadmill running with gradual increase in intensity. The blood and gastrocnemius muscles (white and red portion) were collected immediately after and 24 h post exercise. For 3 days, imipramine was intraperitoneally injected 1 h prior to treadmill running. [Results] Interleukin 6 (IL-6) and serum creatine kinase (CK) levels were enhanced immediately after and 24 h post exercise (relative to those of resting), respectively. Acidic sphingomyelinase (A-SMase) protein expression in gastrocnemius muscles was significantly augmented by exercise, unlike, serine palmitoyltransferase-1 (SPT-1) and neutral sphingomyelinase (N-SMase) expressions. Furthermore, imipramine (a selective A-SMase inhibitor) treatment reduced the exercise-induced CK and IL-6 elevations, along with a decrease in cleaved caspase-3 (Cas-3) of gastrocnemius muscles. [Conclusion] We found the crucial role of A-SMase in exercise-induced muscle damage.

• Clinical Pain
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• v.18 no.1
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• pp.48-51
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• 2019

Symmetrical proximal weakness and characteristic dermatologic manifestations are important in the diagnosis of dermatomyositis. We report a case of atypical presentation of dermatomyositis due to previous steroid use and also report steroid-induced myopathy which may occur from steroid administration during the course of treatment. A 77-year-old man, previous steroid user, showed rapidly progressing weakness after abruptly stopped medication. He has presented erythematous papule on face and anterior chest but no heliotrope rash and Gottron's papules were observed. Muscle enzyme (creatine kinase) concentration is increased, and needle electromyography shows increased spontaneous activity on proximal limb muscle. The muscle biopsy confirmed dermatomyositis. During the course of treatment, he revealed persistent weakness despite the continuous steroid use and stable creatine kinase level. Electrodiagnostic study suggests steroid-induced myopathy and after tapering steroid, proximal muscle strength improved. This case reports the effect of steroid use on dermatomyositis patients and a process of diagnosing coexisting steroid induced myopathy during treatment.

• Journal of Ginseng Research
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• v.48 no.2
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• pp.113-121
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• 2024

Since its outbreak in late 2019, the Coronavirus disease 2019 (COVID-19) pandemic has profoundly caused global morbidity and deaths. The COVID-19 pandemic caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) has major complications in cardiovascular and pulmonary system. The increased rate of mortality is due to delayed detection of certain biomarkers that are crucial in the development of disease. Furthermore, certain proteins and enzymes in cellular signaling pathways play an important role in replication of SARS-CoV-2. Most cases are mild to moderate symptoms, however severe cases of COVID-19 leads to death. Detecting the level of biomarkers such as C-reactive protein, cardiac troponin, creatine kinase, creatine kinaseMB, procalcitonin and Matrix metalloproteinases helps in early detection of the severity of disease. Similarly, through downregulating Renin-angiotensin system, interleukin, Mitogen-activated protein kinases and Phosphoinositide 3-kinases pathways, COVID-19 can be effectively controlled and mortality could be prevented. Ginseng and ginsenosides possess therapeutic potential in cardiac and pulmonary complications, there are several studies performed in which they have suppressed these biomarkers and downregulated the pathways, thereby inhibiting the further spread of disease. Supplementation with ginseng or ginsenoside could act on multiple pathways to reduce the level of biomarkers significantly and alleviate cardiac and pulmonary damage. Therefore, this review summarizes the potential of ginseng extract and ginsenosides in controlling the cardiovascular and pulmonary diseases by COVID-19.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.6
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• pp.834-841
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• 2019

Objective: This study was conducted to investigate the effects of in ovo feeding (IOF) of creatine pyruvate (CrPyr) on the energy metabolism in thigh muscle of embryos and neonatal broilers. Methods: A total of 960 eggs were randomly assigned to three treatments: i) non-injected control group, ii) saline group injected with 0.6 mL of physiological saline (0.75%), and iii) CrPyr group injected with 0.6 mL of physiologi-cal saline (0.75%) containing 12 mg CrPyr/egg on 17.5 d of incubation. After hatching, 120 male chicks (close to the average body weight of the pooled group) in each group were randomly assigned to eight replications. The feeding experiment lasted 7 days. Results: The results showed that IOF of CrPyr increased glucose concentrations in the thigh muscle of broilers on 2 d after injection (p<0.05). Compared with the control and saline groups, the concentration of creatine in CrPyr group was increased on 2 d after injection and the day of hatch (p<0.05). Moreover, IOF of CrPyr increased the creatine kinase activity at hatch and increased the activities of hexokinase and pyruvate kinase on 2 d after injection and the day of hatch (p<0.05). Chicks in CrPyr group showed higher mRNA expressions of glucose transporter 3 (GLUT3) and GLUT8 on the day of hatch (p<0.05). Conclusion: These results demonstrated that IOF of CrPyr was beneficial to enhance muscle energy reserves of em-bryos and hatchlings.

• The Korean Journal of Zoology
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• v.34 no.3
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• pp.420-425
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• 1991

본 연구는 인삼 사포닌(Ponax ginseng C.A. Meyer)이 수영운동을 부하시킨 웅성 횐쥐(Sprague-Dawley 계, 360 $\pm$ 40 9)의 대퇴근 크레아틴 키나제(Creative Kinase, CK: E.C. 2.7.3.2) 동위효소 활성에 미치는 영향을 연구하였다. 운동군은 3시간 동안 수영운동시켰으며 ,인삼군은 인삼 사포닌을 체중 Kg 당 120 mg 복강투여하였다. 인삼 사포닌을 투여한 운동군은 수영운동 1시간 전에 인삼 사포닌을 투여한 후 3시간 동안 수영시켰다. 인삼군의 MB-CK의 활성은 대조군보다 유의하게 증가하였다(P<0.01). 또한 인삼 비투여 운동군과 인삼 투여 운동군에서의 MM-CK활성은 대조군보다 각각 현저하게 증가하였고(P < 0.01와 P < 0.05), 특히 인삼 투여 운동군에서 BB-CK활성이 대조군보다 유의하게 증가하였다(P < 0.01). 인삼 사포닌은 일반적으로 CK-동위효소의 활성을 증가시키고, 운동은 MM-CK의 활성을 현저하게 증가시켰으며, 인삼 투여 운동군에서 BB-CK의 활성이 인삼 비투여 운동군보다 현저하게 증가하였다. 따라서 인삼 사포닌과 운동이 CK동위효소 활성에 상승의 효과를 나타내는 것으로 생각된다.

• Journal of Life Science
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• v.22 no.6
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• pp.713-722
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• 2012

When proteins extracted from longissimus dorsi muscles of Landrace and Berkshire at the finishing stage were compared by 2-DE, the Landrace demonstrated a quantitative increase in proteins related to slow skeletal muscle function, such as serum albumin precursor, troponin T (slow skeletal muscle; sTnT) and myoglobin. In contrast, the Berkshire exhibited comparatively elevated enzymes involved in metabolic pathways, fast skeletal muscle function, and energy production, such as heat shock 27-kDa protein (HSP27)-1, TnT (fast skeletal muscle; fTnT), muscle creatine kinase, phosphoglucomutase 1 (PGM1), triosephosphate isomerase (Tpi1) and adenylate kinase isoenzyme 1 (AK1). When compared to growing Berkshire, finishing Berkshire showed increased levels of aldehyde dehydrogenase 1 family, member L1 (ALDHL1), and muscle creatine kinase. In contrast, the growing Berkshire muscle had elevated levels of HSP27-1, sTnT, fTnT, serum albumin precursor, PGM1, AK1, and Tpi 1 as compared to the finishing Berkshire. The Landrace longissimus dorsi muscle may be composed of slower skeletal muscle, whereas Berkshire is composed of a faster skeletal muscle. The uniquely elevated quantities of proteins involved in skeletal muscle function, energy metabolism, and cytoskeleton function in the growing Berkshire indicate that these factors support growth and maintenance during the growing stage when compared with the finishing Berkshire.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.6
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• pp.2632-2640
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• 2012

The purpose of this study was to investigate whether medial gastrocnemius ultrasound imaging of the Delayed Onset Muscle Soreness (DOMS) has the possibilities as a measurement method. This study was conducted from April 21th 2011 to April 30th 2011. Thirty-five healthy subjects were included based on the absence of regular physical activity, and no history of recent trauma, musculoskeletal pathology, cardiovascular disease or drug intake. All subjects induced DOMS through climbing for 5 hours and we measured the visual analogue scale (VAS), creatine kinase (CK) and maximal voluntary isometric contraction (MVlC) of ankle plantar flexor prior to DOMS and at 24, 48 and 72 hours post DOMS and these measurements were compared with pennation angle of medial gastrocnemius measured by ultrasound imaging. Results of this study were as following. VAS, CK, and MVIC of ankle plantar flexor were found significant difference related measurement period (p<0.05) and pennation angle of medial gastrocnemius were found significant difference related measurement period (p<0.05). Furthermore, we confirmed that the flow of change between variables related measurment period was consistent. Through this study, we think that measuring the changes in pennation angle of medial gastrocnemius over time using ultrasound imaging will be able to be used as a new method measuring DOMS.