• The Korean Journal of Malacology
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• v.27 no.1
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• pp.35-42
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• 2011

In order to understand effect of polycyclic aromatic hydrocarbon (PAH) on shell repair of the Pacific oyster, Crassostrea gigas, shell regeneration experiments were carried out using oysters drilled a hole on the right valve. The change of pH and hemocytic characteristics in both extrapallial fluid and hemolymph were observed during the shell repair. The thickness of mantle tissue was apparently decreased, while necrosis in epithelium and periostracal gland was increased in response to PAH exposure. Our finding suggested that PAH could adversely influence on shell repair.

• The Korean Journal of Malacology
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• v.27 no.3
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• pp.223-228
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• 2011

To understand the role of hemocytes in the shell repair process, a hole was drilled in the right valves of the Pacific oyster, Crassostrea gigas, and the repair process was observed. Histological observations suggested that the exterior surface of the shell was repaired by aggregated hemocytes. The nuclei of the hemocytes were cleary stained in the regenerated shell while appearing fragmented after calcification at the $7^{th}$ day. Globular calcium granules were genegenerated from the hemocytic monolyer after 6 days of incubation which were morphologically and chemically identical with those from prismatic shell. Our finding suggested that the repaired prismatic shell was composed by aggregated hemocytes and that their endogenous calcium component might support the nucleation of calcium biomineralization during shell repair.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.449-450
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• 2000

이매패의 hemocytes는 상처 치유, shell repair, 영양물질의 소화, 수송 그리고 분비에 관여한다고 알려져 있으며, 체내 방어 기능에서 필수적이다(Carmen et al., 1997). 패류의 hemocytes에 관해서는 주로 형태, 세포화학적 특징이 연구되어 왔으며(Pipe, 1990 등), 밀도차원심분리법(Bachere et al., 1988)으로 분리된 hemocytes를 면역학적 방법에 의해(Cheng et al., 1980; Yoshino and Granath, 1983) 여러 가지 subpopulatione으로 구분하지만, 현재로서는 같은 종의 패류에서도 분류 방법에 따라 다양하게 분류되고 있다. (중략)