• Bulletin of the Korean Chemical Society
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• v.35 no.9
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• pp.2781-2786
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• 2014

Human ${\alpha}_1$-antitrypsin (${\alpha}_1$-AT) is a natural inhibitor of neutrophil elastases and has several dozens of genetic variants. Most of the deficient genetic variants of human ${\alpha}_1$-AT are unstable and cause pulmonary emphysema. However, the most clinically significant variant, Z-type ${\alpha}_1$-AT, exhibits retarded protein folding that leads to accumulation of folding intermediates. These aggregate within the endoplasmic reticulum (ER) of hepatocytes, subsequently causing liver cirrhosis as well as emphysema. Here, we studied the role of an ER folding assistant protein Cpr5p on Z-type ${\alpha}_1$-AT folding. Cpr5p was induced > 2-fold in Z-type ${\alpha}_1$-AT-expressing yeast cells compared with the wild type. Knockout of CPR5 exacerbated cytotoxicity of Z-type ${\alpha}_1$-AT, and re-introduction of CPR5 rescued the knockout cells from aggravated cytotoxicity caused by the ${\alpha}_1$-AT variant. Furthermore, Cpr5p co-immunoprecipitated with Z-type ${\alpha}_1$-AT and facilitated its protein folding. Our results suggest that protein-folding diseases may be suppressed by folding assistant proteins at the site of causal protein biosynthesis.

• Journal of Korean Public Health Nursing
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• v.37 no.2
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• pp.193-204
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• 2023

Purpose: This study was undertaken to identify the association between CPR knowledge, attitude, and teacher efficacy on the performance confidence of CPR. We further investigated methods to enhance the performance ability of CPR among elementary, middle, and high school teachers. Methods: The study design was a descriptive survey conducted from October 15 to December 31, 2022, enrolling 155 elementary, middle, and high school teachers. The data were analyzed by applying Multiple regression, Pearson's correlation coefficient, one way ANOVA and Scheffé test using the SPSS/WIN 23.0 program. Results: The performance confidence of CPR among elementary, middle, and high school teachers showed a significant positive correlation with CPR knowledge (r=.49, p<.001), CPR attitude (r=.26, p<.001), and teacher efficacy (r=.25, p=.002). The factors affecting performance confidence in CPR were CPR knowledge (β=.49, p<.001), gender (β=-.26, p<.001), CPR attitude (β=.20, p=.003), health status (β=.14, p=.038), and teacher efficacy (β=.14, p=.032); these factors explained 40.5%. Conclusion: Results from the study indicate that performance confidence of CPR can be enhanced by providing frequent education on CPR knowledge and attitudes. Moreover, educational programs will aid in maintaining good health and enhance teacher efficacy.

• The Korean Journal of Emergency Medical Services
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• v.9 no.2
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• pp.169-180
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• 2005

The purpose of this research was to evaluate retention of cognitive knowledge, psychomotor skills and self-confidence on CPR 3 months after CPR training program. The sample consisted of 39 nursing students. We provided one rescuer CPR training program for nursing college students on the basis of AHA. The questionnaires for knowledge of CPR were developed 50 items based on AHA guidelines. Self-confidence were checked by 11 items questionnaires. The accuracy of CPR skills were checked by Skillreporter CPR training manikin and by researcher's evaluation based on CPR skill checklist. The results were as follows ; 1. The majority of participants didn't have any previously experiences of CPR training (76.9%). Only 15.1% previously took the CPR training with CPR practice. 2. In terms of self-confidence of CPR. The score were increased for 2 days (p>.001) but retention of self confidence was significantly statistical decreased in 3 months after training (p<.001). 3. There was a statistically significant decrement in mean of knowledge of CPR between 2 days and 3 months after CPR training (p<.001). 4. There was a statistically significant decrement in cognitive knowledge of CPR based on CPR skills checklist(p<.001). 5. Retention scores of psychomotor skills of CPR 3 months after training were 42.10% in numbers of adequate ventilation, 52.81% in numbers of adequate chest compression (p<.001) respectively. 6. Retention of passing rate on chest compressions of CPR 3 months after training was 27% (p<.001), on ventilation was 2.63% (p>.001). The error items with statistically significant differences 3 months after CPR training were too little ventilation (74.36%) and too little chest compressions (92.31%). The results of the study suggest that we need further evaluation of course components which could improve retention of CPR for all trainees.

• The Korean Journal of Emergency Medical Services
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• v.10 no.1
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• pp.5-12
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• 2006

Purpose: Few data exist regarding the cardiopulmonary resuscitation(CPR) education in relationship to characteristics of socio-economic status and health-medical conditions in Korea. The purpose of this study is to describe and analyze the characteristics of which laypersons want the CPR education. Methods: Based on a health survey of Incheon Metropolitan City adults(n=5,114), tests of the differences between the group that wants the CPR (n=1318) and the group that doesn't (n=3576), and a logistic regression analysis of two groups was performed on socio-economic status-gender, age, marital stats, education level, numbers of family members, and monthly household income-and health-medical conditions-diseases, accident experience, EMS(emergency medical system) experience, and health status. Results: Even the participation rate of the CPR education in Korea is only 4.3%, which is extremely lower than other developed countries, there are statistically significant differences between the group that wants the CPR and the group that doesn't on gender(p=0.005), age(p=0.000), education level(p=0.000), numbers of family members(p=0.000), monthly income(p=0.000). diseases(p=0.000). health status(p=0.042). Furthermore, age(OR: 1.025, p=0.000), education level(OR : 0.721, p=0.000), numbers of family members(OR: 0.809, p=0.000), and health status(OR: 1.077, p=0.000) are statistically significant factors on the wish for the CPR education. Conclusion: Accident and EMS experiences are not so much influential factors on that laypersons want the CPR education in Korea. Therefore, certain subgroups of laypersons such as high-risk patients and family members need targeted outreach programs in CPR education.

• BMB Reports
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• v.38 no.3
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• pp.366-369
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• 2005

NADPH-cytochrome P450 reductase (CPR) transfers electrons from NADPH to cytochrome P450 and also catalyzes the one-electron reduction of many drugs and foreign compounds. Various spectrophotometric assays have been performed to examine electron-accepting properties of CPR and its ability to reduce cytochrome $b_5$, cytochrome c, and ferricyanide. In this report, reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) by CPR has been assessed as a method for monitoring CPR activity. The principle advantage of this substance is that the reduction of MTT can be assayed directly in the reaction medium by a continuous spectrophotometric method. The electrons released from NADPH by CPR were transferred to MTT. MTT reduction activity was then assessed spectrophotometrically by measuring the increase of $A_{610}$. MTT reduction followed classical Michaelis-Menten kinetics ($K_m\;=\;20\;{\mu}M$, $k_{cat}\;=\;1,910\;min^{-1}$). This method offers the advantages of a commercially available substrate and short analysis time by a simple measurement of enzymatic activity of CPR.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.6
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• pp.381-388
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• 2017

The CPR guidelines emphasize the delivery of effective chest compressions but do not address the effects of chest compressions on CPR providers. This study determined the effects of chest compressions on healthy adult firefighters' symptoms, hemodynamics, and electrocardiography after performing multiple cycles of CPR. Healthy adult firefighters were trained in CPR and performed CPR on mannequins. The provider vital signs, electrocardiography, and fatigue scores were determined immediately before CPR, after 5cycles of CPR, and after 10 cycles of CPR. In addition, the presence of clinical symptoms among the providers was determined after CPR; 39 firefighters participated in the study. Their mean age was $35.54{\pm}10.26years$. Many providers developed fatigue, shortness of breath, and dizziness. Significant changes in heart rate (p=0.000), respiratory rate (p=0.010), end-tidal CO2(p=0.000), O2 saturation(p=0.000), and pulse pressure (p=0.000) were observed after both 5 and 10 cycles of CPR. One participant developed sinus dysrhythmia and premature ventricular contractions after 10 cycles of CPR. The delivery of chest compression results in fatigue and hemodynamic alterations in many young healthy adults after performing 5 or 10 cycles of CPR. The CPR guidelines and education should take into consideration the effects of chest compressions on CPR providers.

• Journal of Korean society of Dental Hygiene
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• v.16 no.6
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• pp.1067-1078
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• 2016

Objectives: The aim of this study was to investigate the effect of cardiopulmonary resuscitation (CPR) education in dental hygiene students. Methods: A self-reported questionnaire was completed by fifty dental hygiene students in May 2016 before and after CPR education. The questionnaire consisted of knowledge, attitude, confidence, and performance of CPR skill. Performance test by professionals was measured to evaluate capability of skill after CPR education. Results: Scores of the knowledge increased from 4.35 to 12.24 after education (p<0.001). Scores of attitude increased from 51.86 to 58.92 (p<0.001). Scores of confidence changed from 56.66 to 85.02 (p<0.001). Scores of self-capability of skill increased from 44.90 to 74.02 (p<0.001). 5. The average score of capability of skill by professional test was 24.47. Conclusions: CPR education improved the knowledge, attitude, confidence and capability of skill in dental hygiene students. The development and education of CPR program will make the students perform bystander CPR in the emergency situation actively.

• Journal of the Korean Society of Safety
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• v.30 no.5
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• pp.74-79
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• 2015

The purpose of this study is to find out its effect on changes in the joint angle and the success rate of cardiopulmonary resuscitation by conducting cardiopulmonary resuscitation (CPR) when wearing and not wearing PPE (personal protective equipment) targeting 20 paramedics with more than 5 years of experience. The subjects carried out CPR in 30:2 for 4 minutes and collected images were digitized by Kwon3D XP Software Package(Version 4.0) and then data were obtained. Data, which were collected by analyzing the motion when starting in one cycle, when pressing to the maximum, in the final position (relaxed), were analyzed by using SPSS 18.0. In conclusion, during CPR, the angle of the both shoulder joints was not significant (p>.05) and the angle of the right elbow joint was reduced in all positions and was statistically significant (p<.05) and the angle of the left was significantly reduced in the maximum pressure posture and the final position (p>.05). In the case of the trunk, the angle increased statistically significantly at all stages (p<.01, p<.001). Also, during CPR, the average compression rate was significantly reduced after wearing PPE (p<.05) and average hand escape time by cycle increased statistically significantly (p<.05) but chest compression execution rate at the correct depth did not show any significant difference between the two groups (p>05).

• BMB Reports
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• v.37 no.5
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• pp.629-633
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• 2004

NADPH-cytochrome P450 reductase (CPR) transfers electrons from NADPH to cytochrome P450, and catalyzes the one-electron reduction of many drugs and foreign compounds. Various forms of spectrophotometric titration have been performed to investigate the electron-accepting properties of CPR, particularly, to examine its ability to reduce cytochrome c and ferricyanide. In this study, the reduction of 1,1-diphenyl-2-picrylhydrazyl (DPPH) by CPR was assessed as a means of monitoring CPR activity. The principle advantage of DPPH is that its reduction can be assayed directly in the reaction medium by a continuous spectrophotometry. Thus, electrons released from NADPH by CPR were transferred to DPPH, and DPPH reduction was then followed spectrophotometrically by measuring $A_{520}$ reduction. Optimal assay concentrations of DPPH, CPR, potassium phosphate buffer, and NADPH were first established. DPPH reduction activity was found to depend upon the strength of the buffer used, which was optimal at 100 mM potassium phosphate and pH 7.6. The extinction coefficient of DPPH was $4.09\;mM^{-1}\;cm^{-1}$. DPPH reduction followed classical Michaelis-Menten kinetics ($K_m\;=\;28\;{\mu}M$, $K_{cat}\;=\;1690\;min^{-1}$). This method uses readily available materials, and has the additional advantages of being rapid and inexpensive.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.1
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• pp.480-489
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• 2015

This study aimed to compare the education effect of easily accessible cardiopulmonary resuscitation (CPR) applications on smart-phones. The differences of performance and accuracy of CPR between animation CPR group and modified Pocket CPR group in Reserve Officers' Training Corps students of K university were evaluated by 2010 AHA guidelines. Data were collected from May 19, 2012 to May 20, 2012. There was no statistically significant difference between both of group in the performance and accuracy of CPR. However, the modified Pocket CPR group showed significant increase in the accuracy of chest compression depth (26.4%, p<.05), while the animation CPR group showed significant increase in the accuracy of chest compression location after the education (25.2%, p<.01). In conclusion, the methods using advantage and complementing disadvantage of animation CPR application and Pocket CPR application could help that people could easily access and perform to CPR.