• 제목/요약/키워드: Cowpea mosaic virus (CPMV)

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석량풋콩재배지에서의 동부모자이크바이러스병의 발생도 및 풋콩병해모니터링과 동부모자이크 바이러스의 순화 (Severity of Cowpea mosaic virus and Putkong Disease Monitoring and Purification of Cowpea mosaic virus)

  • 조의규
    • 식물병연구
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    • 제13권1호
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    • pp.30-33
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    • 2007
  • 석량풋콩 품종의 콩밭에서 모자이크 증상이 약하게 나타난 186개의 잎을 채집하여, Cowpea mosaic virus(CPMV)에 대한 ELISA 검정을 하였다. 그 결과 93개의 시료가 양성반응을 보였으며, 이 가운데 55개의 시료는 매우 높은 바이러스 밀도를 나타내고 있었다. 이러한 결과는 석량풋콩 품종의 동부모자이크병은 ELISA 검정, 바이러스 순화, 전자현미경검경 결과와 바이러스 표피 단백질분석 결과 Cowpea mosaic virus에 의한 병해로 확인되었다.

Cowpea mosaic virus from Vegetable Soybeans in Korea

  • Cho, Eui-Kyoo;Lee, Sin-Ho
    • The Plant Pathology Journal
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    • 제19권3호
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    • pp.166-170
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    • 2003
  • Ninety samples showing mosaic symptoms on soybean (Glycine max) cv. Sukryangputkong were collected from the Cheongsongkun area, Kyungbuk province in Korea. Initially, DAS-ELISA was conducted far detection of Soybean mosaic virus (SMV). Negative samples were chosen at random and mechanically inoculated on soybean cv. Buffalo, which reported not to produce mosaic symptoms when mechanically inoculated with SMV. An isolate of SMV, designated as B-1, from Buffalo showing mosaic and mottle symptoms was used for identification and biological characterization of the causal vim. The purified B-1 isolate had spherical particles of approximately 24nm. It positively reacted with the antiserum against Cowpea mosaic virus (CPMV) but not with Cucumber mosaic virus (CMV) and SMV antisera. CPMV was newly isolated from soybean and had been characterized by host range and by serological and electron microscopic methods. Results of this study suggest that CPMV is the possible cause of mosaic disease in vegetable soybean and that based on sympto-matology, a difference between the typical mosaic and rugose symptoms caused by SMV and CPMV was observed. This is first report of CPMV from soybean in Korea.