• Journal of Environmental Science International
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• 제27권12호
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• pp.1241-1247
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• 2018

This study was conducted to evaluate resistance of 10 different cowpea cultivars which were introduced from the United States against two major root-knot nematodes, Meloidogyne arenaria and M. incognita, that affect greenhouse crop cultivation in Korea. The results showed that the resistance of the tested cultivars to the root-knot nematodes varied with the cultivar. Texas Cream 40 showed moderate resistance to M. arenaria whereas the other cultivars were susceptible. Purple Hull Pinkeye and Texas Pinkeye Purple Hull were found to be resistant to M. incognita, whereas Mississippi Silver showed moderate resistance and the other cultivars were susceptible. As the cultivars exhibit resistance to M. arenaria and M. incognita, such cultivars resistant to root-knot nematodes should be considered during the cultivation of cowpea as a green manure crop.

• The Plant Pathology Journal
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• 제39권5호
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• pp.486-493
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• 2023

Cowpea mild mottle virus (CPMMV) is a global plant virus that poses a threat to the production and quality of legume crops. Early and accurate diagnosis is essential for effective managing CPMMV outbreaks. With the advancement in isothermal recombinase polymerase amplification and lateral flow strips technologies, more rapid and sensitive methods have become available for detecting this pathogen. In this study, we have developed a reverse transcription recombinase polymerase amplification combined with lateral flow strips (RT-RPA-LFS) method for the detection of CPMMV, specifically targeting the CPMMV coat protein (CP) gene. The RT-RPA-LFS assay only requires 20 min at 40℃ and demonstrates high specificity. Its detection limit was 10 copies/µl, which is approximately up to 100 times more sensitive than RT-PCR on agarose gel electrophoresis. The developed RT-RPA-LFS method offers a rapid, convenient, and sensitive approach for field detection of CPMMV, which contribute to controlling the spread of the virus.

• Korean Journal of Soil Science and Fertilizer
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• 제25권4호
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• pp.378-386
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• 1992

This experiment was conducted to investigate the effects of inoculation of the Bradyrhizobium sp.(Arachis) HCR-46 and fertilizer nitrogen application on nodulation, $N_2$-fixation of cowpea group legume(Seowon dongbu, Seonwha nogdu, Chungju pat, Youngho tangkong). Treatments consisted of five uninoc.+N : O, uninoc.+N : 6kg/10a. inoc.+N : O, inoc.+ N : 6kg/10a, inoc.+N : 12kg/10a. 1. Initial nodulation time of cowpea group legume by inoculation of Bradyrhizobium sp. HCR-46 was 12~21 days, and it was delayed as more fertilizer nitrogen was added. 2. The number and dry weight of nodule were increased as growth period elapsed, but decreased as fertilizer nitrogen was added. 3. Dry matter production and amount of nitrogen uptake were increased by inoculation and the effects of inoculation were increased when fertilizer nitrogen of 6kg/10a was applied. 4. Amount of fixed nitrogen by inoculation of fertilizer nitrogen not applied was higher than the case of 6kg/10a applied and that of peanut was higher than the other legumes(cowpea, mung bean and adzuki).

• Korean journal of food and cookery science
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• 제3권1호
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• pp.31-36
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• 1987

The purpose of this study is to investigate the physicochemcal Properties of the cowpea crude and refined starch and to present the basic data for physicochemical factor which gives the properties of Mook to cowpea starch gel. Water binding capacity of crude starch was 235. In and that of refined starch was 186.0%. The pattern of change in swelling power and solubility for increasing temperature started to increase at $60^{\circ}C$ and increased rapidly from $70^{\circ}C$, for both of crude and refined starch. The optical transmittance of 0.2% crude and refined starch suspensions were increased from $65^{\circ}C$ and showed rapid increasement during 68~$80^{\circ}C$, and their curves showed two-stage processes. The gelatinization pattern for 6n crude and refined starch suspensions were investigated by the Brabender amylograph. The corves showed the pasting temperature of $72.0^{\circ}C$ and $72.1^{\circ}C$, peak height of 11303.U. ($88.0^{\circ}C$) and 970 B.U. ($83.5^{\circ}C$) for crude and refined starch, respectively, and both showed high viscosities when cooling. Blue values for crude and refined starch were 0.369 and 0.376 respectively. Alkali number of crude and refined starch were 7.77 and 7.34, and reducing values were 3.60 and 2. 10, respectively. Amylose content of cowpea starch was 33.7%. Periodate oxidation of the starch fractions resulted that amylose had the average molecular weight of 23590, degree of polymerization of 146 and amylopectin had the degree of branching of 3.42, glucose unit per segment of 29.

• Journal of Food Hygiene and Safety
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• 제29권4호
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• pp.363-369
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• 2014

This study was conducted to investigate the quality characteristics of tofu prepared with addition of cowpea meal (CPM). Any significant difference of L-value between control CPM-O and treatment CPM-I, CPM-II and CPM-III could not be seen as addition ratio of CPM increases (p < 0.05). The b-value of CPM-III (75%) has shown the least value of 14.26, and it has decreased as addition ratio of CPM increases. The result of texture profile did not show any significant difference between control CPM-O and CPM-Iexcept in the case of cohesiveness. The pH range of treatments was 6.36~6.52, which showed no significant difference from controls. The values of turbidity were 1.46~1.81, which had increasing trend as addition ratio of CPM increases. The total bacterial count of CPM-III was $4.40{\times}10^2CFU/g$, which showed significant difference from the control. No Esterichia coli was detected in the control and the treatments. The result of sensory analysis of the treatment CPM-I did not show any significant difference from the control, which implied the treatment CPM-Iwas found to be suitable at the tofu making using byproducts of cowpea-jelly making. Isoflavone glycoside (IG) was contained more on the order of genistin, daidzin, and glycitin, and the content decreased significantly as addition ratio of CPM increases. The content of isoflavone-aglycone (IA) was less than IG, but showed similar behavior of addition ratio of CPM.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 한국식물병리학회 2005년도 한국식물병리학회 임시총회 및 춘계학술발표회
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• pp.54.2-55
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• 2005

• Proceedings of the Korean Society of Crop Science Conference
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• 한국작물학회 2020년도 추계학술대회
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• pp.115-115
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• 2020

• BMB Reports
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• 제40권4호
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• pp.494-500
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• 2007

The endophytic bruchid pest Callosobruchus maculatus causes severe damage to storage cowpea seeds, leading to economical losses. For this reason the use of $\alpha$-amylase inhibitors to interfere with the pest digestion process has been an interesting alternative to control bruchids. With this aim, $\alpha$-amylase inhibitors from baru seeds (Dipteryx alata) were isolated by affinity chromatographic procedures, causing enhanced inhibition of C. maculatus and Anthonomus grandis $\alpha$-amylases. To attempt further purification, this fraction was applied onto a reversed-phase HPLC column, generating four peaks with remarkable inhibition toward C. maculatus $\alpha$-amylases. SDS-PAGE and MALDI-ToF analysis identified major proteins of approximately 5.0, 11.0, 20.0 and 55 kDa that showed $\alpha$-amylase inhibition. Results of in vivo bioassays using artificial seeds containing 1.0% (w/w) of baru crude extract revealed 40% cowpea weevil larvae mortality. These results provide evidence that several $\alpha$-amylase inhibitors classes, with biotechnological potential, can be isolated from a single plant species.

• Journal of The Korean Society of Grassland and Forage Science
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• 제8권1호
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• pp.47-54
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• 1988

The growth characteristics and yield per unit area of two cultivation systems of forage, corn (Zea mays L.) monoculture and corn-cowpea (Vigna sinesis King) inter-cropping were compared and obtained follow results. 1. The two cultivation systems were not significantly different in leaf length, leaf width and length of internode with harvesting time. 2. The weight of stalk and leaves of a plant were decreased with maturity, while weight of ear was markedly increased. The patterns of relative ratio of each components was essentially same in each cultivation system. In corn monocrop., the percentages of stalk, leaf and ear at final harvesting stage were 22.9, 13.7 and 63.4, respectively. 3. Dry matter yield per unit area (33kgIlOa) of intercropped corn at yellow stage was similar to that of monocropped corn ( l,482.8kg/ 10a vs 1 ,SO8.9kg/ 10a). At ripe stage, however, the DM yield of intercrop. was higher than that of monocrop. (1,810.4kg/lOa vs 1,660.4 kg/ 1 Oa). 4. The same pattern was observed in organic matter yield.

• Applied Biological Chemistry
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• 제41권4호
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• pp.222-227
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• 1998

The activities of enzymes of carbohydrate metabolism have been determinated in the host cytosolic and bacteroid fractions of cowpea (Vigna unguiculata) nodules formed with B. japonicum I 16 and in roots of nodulated cowpeas. The host cytosolic fraction of the nodules contained the enzymes of glycolytic pathway and the pentose phosphate pathway, whereas the bacteroids had only limited capacity for carbohydrate metabolism and appeared to be insufficient for the complete glycolytic pathway as well as starch synthesis and degradation. In a time-course study, using plants grown in a glasshouse, the acetylene-reducing activity (ARA) of the nodules increased in parallel with the total N content of plants and protein of the nodules until approximately 8 weeks after planting. Subsequently, the weight and size of the nodules and the weight of the plants continued to increase, but there was a sharp decrease in the ARA and the total N content of the plants.