• Asian-Australasian Journal of Animal Sciences
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• 제3권1호
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• pp.21-25
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• 1990

Fructo-oligosaccharides are found in many plants, such as onion, burdock and wheat. They are not well hydrolyzed by digestive enzymes in animals, but are peculiarly assimilated by Bifidobacterium and some useful bacteria. In our previous experiment (Kobayashi et al., 1987) it was suggested that they were effective in decreasing energy loss in the metabolism of dairy calves. In the present study, the effects of fructo-oligosaccharides on body weight, milk-yield and milk-components (fat, protein and solids-not-fat) were investigated in dairy cows. Lactating cows were fed a standard diet containing fructoligo saccharides at 18.70g, 9.35g and 0.0g (control) per 100kg body weight, day for three weeks. Neither treatments significantly affected any of the parameters examined. The fructo-oligosaccharides were assumed to be hydrolyzed by rumen microorganisms and hardly to affect the bacterium florae in the intestines of the lactating cows.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2005년도 국제학술심포지움 The 44th Annual Meeting of Korean Society for Life Science
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• pp.185-185
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• 2005

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2005년도 국제학술심포지움 The 44th Annual Meeting of Korean Society for Life Science
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• pp.233-233
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• 2005

• Asian-Australasian Journal of Animal Sciences
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• 제22권10호
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• pp.1366-1376
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• 2009

Effect of sulfur (S) on utilization efficiency of fresh cassava foliage and cassava hay in dairy cows was evaluated using thirty-two $1^{st}-2^{nd}$ lactation Holstein-Friesian crossbred dairy cows. The experimental treatment was a 2${\times}$2 factorial arrangement in a randomized complete block design (RCBD) using two roughages (rice straw+fresh cassava foliage (FCF) and rice straw+cassava hay (CH)) and two elemental sulfur (S) levels (0.15 and 0.4% S of dry matter (DM)), respectively. Four dietary treatments (FCF+0.15, FCF+0.4, CH+0.15 and CH+0.4) were offered ad libitum in the form of a total mixed ration (TMR) with concentrate to roughage (chopped rice straw+chopped cassava foliage) ratio at 60:40. Fresh cassava foliage or cassava hay resulted in similar dry mater intake, rumen ecology parameters, total tract digestibility, blood chemistry, milk production and composition. However, HCN intake, blood and milk thiocyanate concentration were significantly higher (p<0.01) in cows fed fresh cassava foliage with no sign of potential toxicity. Dry matter intake, body weight changes, molar percentage of propionate in rumen, neutral detergent fiber (NDF) digestibility and nitrogen (N) retention of cows tended to be increased while DM digestibility (65.6, 72.7, 68.6 and 72.1% of total DM intake for the respective treatments), rumen bacteria population (1.4, 1.7, 1.6 and $1.7{\times}10^{11}$ cell/ml for respective treatments), fungal zoospore population (0.4, 0.6, 0.4 and $0.5{\times}10^{6}$ cell/ml for respective treatments), urinary allantoin (25.3, 28.0, 26.3 and 27.6 g/d for respective treatments), microbial N yield (136.0, 154.6, 142.8 and 151.3 g N/d for respective treatments) and milk protein content (3.4, 3.5, 3.2 and 3.5% for respective treatments) were significantly (p<0.05) higher in cows fed on supplemented sulfur at 0.4% of DM in comparison with 0.15% S-supplemented diets. Based on these results, it is concluded that cassava foliage could be used as a portion of roughage for dairy cows and supplementation of S would be nutritionally beneficial.

• Asian-Australasian Journal of Animal Sciences
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• 제28권3호
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• pp.351-359
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• 2015

Twelve multiparous Holstein dairy cows in mid-lactation were selected for a replicated $4{\times}4$ Latin square design with a $2{\times}2$ factorial arrangement to investigate the effects of corn and soybean meal (SBM) types on rumen fermentation, N metabolism and lactation performance in dairy cows. Two types of corn (dry ground [DGC] and steam-flaked corn [SFC]) and two types of SBM (solvent-extracted and heat-treated SBM) with different ruminal degradation rates and extents were used to formulate four diets with the same basal ingredients. Each period lasted for 21 days, including 14 d for adaptation and 7 d for sample collection. Cows receiving SFC had a lower dry matter (DM) and total N intake than those fed DGC. However, the milk yield and milk protein yield were not influenced by the corn type, resulting in higher feed and N utilization efficiency in SFC-fed cows than those receiving DGC. Ruminal acetate concentrations was greater and total volatile fatty acids concentrations tended to be greater for cows receiving DGC relative to cows fed SFC, but milk fat content was not influenced by corn type. The SFC-fed cows had lower ruminal ammonia-N, less urea N in their blood and milk, and lower fecal N excretion than those on DGC. Compared with solvent-extracted SBM-fed cows, cows receiving heat-treated SBM had lower microbial protein yield in the rumen, but similar total tract apparent nutrient digestibility, N metabolism measurements, and productivity. Excessive supply of metabolizable protein in all diets may have caused the lack of difference in lactation performance between SBM types. Results of the present study indicated that increasing the energy degradability in the rumen could improve feed efficiency, and reduce environmental pollution.

• Asian-Australasian Journal of Animal Sciences
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• 제11권6호
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• pp.673-679
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• 1998

The experiment was conducted to determine in situ rumen degradability of soybean meal (SM), fish meal (FM), sesame cake (SC) and Italian ryegrass hay (IRGH) and the effect of supplementing the above protein meals to IRGH on digestibility and balance of nutrients in three Saanen goats. For measuring the degradability, nylon bags containing each meal were incubated in the rumen of one fistulated dry cow for 3, 6, 12, 24, 36, and 48 hrs. Degradability revealed that SM protein was highly degradable in the rumen (99.1%), while FM protein was less degradable (76.8%) with SC protein being intermediate (91.2%) at 48 hrs of incubation (p < 0.01). Degradation rate of the potentially degradable fraction was estimated to be 12.12, 5.88 and 5.88%/hr for SM, FM and SC, respectively. In the metabolism trial, all goats were offered daily 900 g IRGH and one of the supplements, SM (100 g), FM (75 g) or SC (100 g). Intake of DM, OM, CP and GE was similar among diets. However, digestibility of OM for SC diet was significantly lower than that for diets supplemented with FM and SM (p < 0.10). Nitrogen (N) excreted in faeces (p < 0.05) and in urine (p < 0.10) was, respectively, higher and lower for SC diet than that for the other two diets. The same tendency was observed in energy losses in faeces (p < 0.10) and in urine (p < 0.05). There was no difference in energy loss in methane or in heat production among diets. Consequently, no significant difference was observed in N retention (2.13, 0.42 and -0.11 g/day for FM, SC and SM diet, respectively) or in energy retention (-1.49, -2.14 and -2.70 MJ/day for FM, SM and SC diet, respectively). These results showed that protein supplements affected the digestion of diets based on grass hay with 7.45% CP in DM in goats, although there was no significant influence on N or energy retention.

• 한국축산시설환경학회지
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• 제15권2호
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• pp.91-98
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• 2009

본 실험은 국립축산과학원에 초지사료연구센터에서 조사료의 IVDMD 분석을 위해 사용 중인 Holstein을 이용하여, 혼합건초를 자유 채식케 하는 Holstein에서 반추위액의 채취 시간이 사료작물의 IVDMD에 미치는 영향을 알아보기 위하여 실시하였다. 사료작물은 청보리 (Yong-yang, Yu-yeong), 크림손 클로버 (Tibbee), 이탈리안 라이그라스(Kospeed, Kogreen, Hwasan 101), 호밀(Wintergreen) 등 4작물 7품종을 공시재료로 이용하였으며, 반추위액의 채취 시간은 T1: 농후사료 급여 전, T2: 사료급여 직후(급여 1시간 후), T3: 사료급여 후 4시간, T4: 사료급여 후 8시간으로 하였다. 모든 사료작물에서 T1 처리구의 위액을 이용한 IVDMD가 T2, T3, T4 처리구에서 채취한 위액을 이용한 IVDMD 보다 높게 나타났다. 특히 IRG의 Kospeed의 경우 T1 처리구에서 T2 처리구보다, Kogreen의 경우 T1 처리구에서 T2 및 T3 처리구보다 IVDMD이 유의적으로 높게 나타났다(p<0.05). 하지만 나머지 모든 사료작물에서 처리구간에 IVDMD의 차이는 있었지만 유의성은 인정되지 않았다. 이러한 결과는 혼합건초의 자유채식이 사료급여 횟수를 증가시킴과 동시에 조사료를 충분히 급여하는 효과를 가져와 침 분비량이 증가하여, 배합사료 급여 후에도 제 1 위 내 pH가 정상범위를 유지하여 사료작물의 안정적인 소화에 영향을 미친 것으로 생각된다.

• 한국산학기술학회논문지
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• 제19권6호
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• pp.652-657
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• 2018

젖소농가에서 착유생산성에 영향을 미치는 번식관리는 매우 중요한 요소이다. 젖소에서 번식관리는 발정의 조기 발견이 중요하며, 보다 정확하고 신속한 젖소의 발정시기에 대한 신뢰할 수 있는 자동 모니터링이 농가에는 필요한 실정이다. 본 연구에서는 젖소의 발정유무, 활동량변화, 반추위 운동량, 반추위 온도 및 pH 등을 측정하는 기기를 활용하여 발정에 대한 정확도를 측정하였다. 연구에 사용된 생체정보기기 S1(Smaxtec, Austria)는 반추위 내 삽입형타입으로 반추위의 온도, pH, 젖소의 활동량 및 음수횟수 추정 등을 이용하여 발정 유무의 알림을 제공하고, S2(Healthy Cow 24, SCR. Allflex. Israel)는 목걸이 타입으로 반추위 운동량, 젖소의 활동량으로 발정의 유무를 알려준다. 기기에서 수집되는 데이터는 1일 2시간 간격으로 획득하였고, 각각의 기준일(Reference day: RD ; -7~-3, +7~+3)은 수정일(-2, -1, 0, +1, +2)이 5일과 수정 전 7일에서 수정 후 7일에 대한 평균값을 기준으로 비교하였다. 본 논문에서 사용된 S1 기기의 활동성은 기준일(RD : $10.2{\pm}1.0/day$)에 비해 수정일(-1 : $12.5{\pm}1.03/day$ ;0 : $12.9{\pm}1.73/day$)에서 증가 하는 것으로 나타났다. S2 기기의 활동성 또한 기준일 (RD : $40.3{\pm}2.68$)에 비해 수정일(0 : $63.0{\pm}3.66$)에서 증가 하는 것으로 분석되었다. S1의 일일 음수횟수는 기준일(RD : $5.9{\pm}0.89/day$)에서 수정일 전까지는 (-2 : $5.6{\pm}0.98$ ; -1 : $5.7{\pm}0.96$)으로 감소하는 것으로 나타났으나, 수정일(0 : $6.3{\pm}0.86$ ;+2 : $6.0{\pm}0.73$)에는 기준일과 같은 횟수를 보였다. S2의 일일 반추횟수는 기준일(RD : $493.8{\pm}10.92$) 보다 수정일(-1 : $390.2{\pm}13.36$; 0 : $354.1{\pm}16.71$)에 감소하는 것으로 나타났다.

• Journal of Animal Science and Technology
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• 제48권1호
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• pp.77-90
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• 2006

본 연구는 꽃사슴과 Holstein 젖소의 반추위와 대장에 서식하는 미생물중 섬유소 분해력이 강한 혐기성 박테리아를 순수 분리하여 분리된 미생물들을 동정하고 이들 미생물들의 효소 특성을 구명하고자 수행되었다. 배지의 종류에 관계없이 젖소에서 분리된 박테리아가 꽃사슴에서 분리된 미생물에 비하여 섬유소 분해효소 활력이 우수하였고 탄소 공급원의 종류에 의해 섬유소 분해 효소의 활력에 영향을 미쳤으며 특히, cellulose 단독 공급시 보다 starch, glucose와 cellobiose를 복합한 탄소 공급원을 제공시 일반적으로 높은 효소의 활력을 나타내었다. API kit를 이용한 생화학 및 당발효 시험 결과 알려진 강력한 섬유소 분해 박테리아는 동정되지 않았고 대부분의 박테리아가 Peptostreptococcus spp., Bifidobacterium spp., Prevotela ruminicola/buccae, Clostridium beijer/butyricum 및 Streptococcus intemedis로 동정되었다. 분리된 균들의 다당류 및 단당류를 분해할 수 있는 가수분해 효소인 Avicelase, xylanase, β-D-glucosidase, α-L-arabino- furanosidase 및 β-xylosidase의 효소활력은 이용하는 배지조성 특히 탄소 공급원의 종류에 의하여 효소의 활력에 영향을 미치며 가수분해 효소의 종류에 따라 각 분리된 균주들마다의 다른 분포를 나타내었다. 결론적으로 분리된 혐기성 박테리아들이 공급되는 탄수화물 기질의 종류에 따라 효소의 활력에 변화를 일으켰고 이것은 기질에 따른 박테리아의 효소생산 특이성과 성장률의 변화에서 기인하였기 때문이다.

• Asian-Australasian Journal of Animal Sciences
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• 제30권10호
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• pp.1416-1424
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• 2017

Objective: The objective of this study was to examine the effects of alfalfa flavonoids on the production performance, immunity, and ruminal fermentation of dairy cows. Methods: The experiments employed four primiparous Holstein cows fitted with ruminal cannulas, and used a $4{\times}4$ Latin square design. Cattle were fed total mixed ration supplemented with 0 (control group, Con), 20, 60, or 100 mg of alfalfa flavonoids extract (AFE) per kg of dairy cow body weight (BW). Results: The feed intake of the group receiving 60 mg/kg BW of AFE were significantly higher (p<0.05) than that of the group receiving 100 mg/kg BW. Milk yields and the fat, protein and lactose of milk were unaffected by AFE, while the total solids content of milk reduced (p = 0.05) linearly as AFE supplementation was increased. The somatic cell count of milk in group receiving 60 mg/kg BW of AFE was significantly lower (p<0.05) than that of the control group. Apparent total-tract digestibility of neutral detergent fiber and crude protein showed a tendency to increase (0.05<$p{\leq}0.10$) with ingestion of AFE. Methane dicarboxylic aldehyde concentration decreased (p = 0.03) linearly, whereas superoxide dismutase activity showed a tendency to increase (p = 0.10) quadratically, with increasing levels of AFE supplementation. The lymphocyte count and the proportion of lymphocytes decreased (p = 0.03) linearly, whereas the proportion of neutrophil granulocytes increased (p = 0.01) linearly with increasing levels of dietary AFE supplementation. The valeric acid/total volatile fatty acid (TVFA) ratio was increased (p = 0.01) linearly with increasing of the level of AFE supplementation, the other ruminal fermentation parameters were not affected by AFE supplementation. Relative levels of the rumen microbe Ruminococcus flavefaciens tended to decrease (p = 0.09) quadratically, whereas those of Butyrivibrio fibrisolvens showed a tendency to increase (p = 0.07) quadratically in response to AFE supplementation. Conclusion: The results of this study demonstrate that AFE supplementation can alter composition of milk, and may also have an increase tendency of nutrient digestion by regulating populations of microbes in the rumen, improve antioxidant properties by increasing antioxidant enzyme activities, and affect immunity by altering the proportions of lymphocyte and neutrophil granulocytes in dairy cows. The addition of 60 mg/kg BW of AFE to the diet of dairy cows was shown to be beneficial in this study.