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Effect of Pine Silage Feeding on Nutrient Digestibility, Feed Conversion and Carcass Traits of Korean Native Cattle (잣나무 生枝葉사일리지 급여가 한우의 영양소 소화율, 사료요구율 및 도체성적에 미치는 영향)

  • 오영균;정찬성;이상철;김경훈;최창원;강수원;문여황
    • Journal of Animal Science and Technology
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    • v.48 no.2
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    • pp.219-226
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    • 2006
  • The wastes (the needle leaves and branches) produced from thinning the forest were fermented under an anaerobic condition (pine silage) to utilize as a forage source of ruminants. An in situ trial was conducted with two ruminally fistulated Korean native cows by 4 replicates (2 bags per cow), and in vivo digestibility of pine silage was estimated with five Korean native steers by 5 replicates in incomplete double turn-over design. In order to investigate feed efficiency and carcass traits, forty eight Korean native bulls were assigned to four treatments (0%, 25%, 50%, 75% of pine silage) with a completely randomized design in 12 pens accommodating 4 animals per pen. The amounts of concentrate and roughage allowed to experimental animals were in the range of 2.5% and 0.6% of body weight, respectively. Animals had freely accessed to mineral block and water in stanchion barn.An in situ crude protein digestibility of the roughage sources when suspended for 48 hrs in the rumen was higher for feeding the pine silage than the rice straw, whereas NDF digestibility was vice versa. No differences between the treatments were observed in in vivo digestibilities of dry matter, crude protein and NDF. Daily body weight gain was significantly (P<0.01) higher for the pine silage substitutions compared with the rice straw feeding except for the 25% silage feeding. The pine silage feeding did not affect feed efficiency.In carcass traits, marbling score tended to be higher for the 50% pine silage treatment than the other treatments while back fat thickness tended to be the thinnest for the rice straw feeding. From these results, it may be recommended to substitute pine silage, as a forage source, for rice straw by 50 percent.

Effects of Melengesterol Acetate, Selenium and Vitamin E Supplemental Feeding on Growth, Carcass and Longissimus Dorsi Muscle Traits in Hanwoo Cull Cows (한우 노산암소에 대한 발정억제제 및 항산화제의 급여가 증체와 도체성적에 미치는 영향)

  • 정준;이성수;박노형;성낙일;장윤호;최성호;송만강;서형기;이명일
    • Journal of Animal Science and Technology
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    • v.48 no.2
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    • pp.255-268
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    • 2006
  • Twenty four Hanwoo cull cows were assigned to 2 groups, control and melengerol acetate(MGA)+selenium supplement containing vitamin E(SeE), based on the parity(6.5±1.7 birth) and body weight (493.17±55.61kg), and the experiment was conducted to establish the reasonable fattening method of cull cows for 240 days. Average daily body gains during 240 days were 0.51kg and 0.63kg in control and MGA+SeE, respectively(P<0.10). DDMI/ADG of MGA+SeE group improved compared to control group(P<0.05). Therefore, supplementation of MGA+SeE in concentrates may accelerate both of the growth rate and feed efficiency in Hanwoo cull cows. MQI from MGA+SeE was more developed based on the larger rib-eye area and thinner backfat thickness in carcass than that from control. Marbling score for MGA+SeE tended to increase compared to control. Dietary Se supplementation significantly affected muscle Se concentration in longissimus dorsi meat of MGA+SeE group(P<0.05). Similar results to Se were obtained from α-Tocoperol concentration. During 7 days of simulated retail display, accumulations of thiobarbituric acid reactive substances(TBARS) concentration in beef was greater(P<0.05) in control than in supplemented cows. These results supported the hypothesis that supplementation of MGA+SeE improve the growth performance and carcass grade both by the growth stimulating effect of MGA+SeE and by preventing the oxidation of the longissimus dorsi muscle in Hanwoo cull cows.

Effect of the Nutrition Education for Infant Feeding on Mother's Knowledge, Attitude, Practice of Weaning (영유아 섭식에 관한 영양교육이 어머니의 영양지식.태도 및 실천에 미치는 효과)

  • Kim, Mi-Yang;Ahn, Hong-Seok
    • Journal of the Korean Society of Food Culture
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    • v.18 no.4
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    • pp.320-332
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    • 2003
  • This study evaluated the effect of the nutrition education about infant feeding on the change of mother's knowledge, attitude and practice of weaning. The subjects consisted of treatment group (n=54) and control group (n=34). The treatment group participated in 6 times nutrition education and 5 times surveys. Nutrition education for weaning of the treatment group improved nutrition knowledge, attitude and practice in infant feeding. Before education, mean scores of control group and treatment group were 7.3 and 7.5 respectively but there was no significant difference. Both group got low score in a statement about weaning period, but they got good score in a statement about weaning method. After education, treatment group got better score(9.1) than control group(8.3) significantly (p<0.05). Treatment group was more desirable than control group specially in a use of Sun-sik, sugar, salt and weaning food mixed with formula after education (p<0.05). Practice of spoon feeding was more frequent in treatment group after education, however, control group used sugar and commercial weaning food more frequently than treatment group(p<0.05). Intake frequency of cow's milk and cookies of infants at age 9-12 month in control group was higher than treatment groups(p<0.01). Thus the nutrition education to mother seems to be effective in achieving desirable dietary behavior of infants.

Factors Affecting Growth Curve Parameters of Hanwoo Cows (한우 암소의 성장곡선 모수에 영향을 미치는 요인)

  • Lee, C.W.;Choi, J.G.;Jeon, K.J.;Na, K.J.;Lee, C.;Hwang, J.M.;Kim, J.B.
    • Journal of Animal Science and Technology
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    • v.45 no.5
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    • pp.711-724
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    • 2003
  • Some growth curve models were used to fit individual growth of 1,083 Hanwoo cows born from 1970 to 2001 in Daekwanryeong branch, National Livestock Research Institute(NLRI). The effects of year-season of birth and age of dam were analyzed. In analysis of variance for growth curve parameters, the effects of birth year-season were significant for mature weight(A), growth ratio(b) and maturing rate(k)(P〈.01). The effects of age of dam were significant for growth ratio(b) but not significant for mature weight(A) and maturing rate(k). The linear term of the covariate of age at the final weights was significant for the A(P〈.01) and k(P〈.01) of Gompertz model, von Bertalanffy model and Logistic model. For the growth curve parameters fitted on individual data using Gompertz model, von Bertalanffy model and Logistic model, resulting the linear contrasts(fall-spring), Least square means of A in three nonlinear models were higher cows born at fall and A of Logistic model was significant(P〈.05) between the seasons. According to the results of the least square means of growth curve parameters by age of dam, least square means of mature weight(A) in Gompertz model was largest in 6 year and smallest estimating for 3 and 8 years of age of dam. The growth ratio(b) was largest in 2 year of age of dam and smallest estimating in 8 year. The A and k were not different by age of dam(p〉.05), On the other hand, the b was different by age of dam(p〈.01). The estimate of A in von Bertalanffy model was largest in 6 year and smallest in 8 and 9 years of age of dam. The b was largest in 2 year and tend to decline as age of dam increased. The A and k were not different by age of dam(p〉.05), On the other hand, the b was highly significant by age of dam(p〈.01).

Estimation of Genetic Parameters of Body Weight by Growth Periods for Hanwoo Using Animal Model (개체모형에 의한 한우의 성장단계별 체중의 유전모수 추정)

  • Choi, J.G.;Jeon, K.J.;Lee, C.W.;Na, G.J.;Lee, C.;Kim, J.B.
    • Journal of Animal Science and Technology
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    • v.45 no.5
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    • pp.667-678
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    • 2003
  • The objectives of this study were to investigate the genetic characteristics of body weight by growth periods for Hanwoo. A total of 1,736 records were used for body weight. The data for body weights were collected from 1990 to 2000 in Daekwanryong branch, National Livestock Research Institute(NLRI). Estimates of (co)variance components were obtained by derivative-free Restricted Maximum Likelihood (DF-REML). The results are summarized as follows; The means for the weights were 25.60, 79.31, 98.91, 145.40, 283.26, 392.32, 545.65kg at birth, 3, 4, 6, 12, 18, 24month postpartum, respectively. The effects of calving year-season were significant for the milk yield of cow. Heritability estimates of direct genetic effects for birth weight were 0.54(all), 0.52(female), 0.36(male) in modelⅠ, 0.45(all), 0.41(female), 0.24(male) in modelⅡ, and heritabilities estimates of direct genetic effects for 4 month(weaning) weight were 0.47(all), 0.33(female), 0.28(male) in modelⅠ, 0.38(all), 0.21(female), 0.21(male) in modelⅡ. Heritability estimates for male and female data differed from those for combined data. The estimates became smaller for the body weights at 12 month or later(0.13~0.05). The heritabilities of average daily gain were smaller than those for body weights, but showed that the similar pattern to body weights.

Research of Statistical Model for Genetic Evaluation of Hanwoo Carcass Traits (한우 도체형질의 유전능력평가를 위한 통계모형 탐색)

  • Koo, Yang-Mo;Kim, Si-Dong;Kim, Jung-Il;Song, Chi-Eun;Lee, Ki-Hwan;Jeoung, Yeoung-Ho;Lee, Jae-Youn;Jang, Hyun-Gi;Park, Byoung-Ho;Choi, Te-Jong;Cho, Kwang-Hyun;Lee, Seung-Soo;Lee, Jung-Gyu;Kim, Hyo-Sun
    • Journal of Animal Science and Technology
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    • v.53 no.4
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    • pp.283-288
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    • 2011
  • This study was conducted to study the environment effects on live weight, carcass weight, dressing percentage, eye muscle area, backfat thickness, and marbling score, which are the carcass traits of Hanwoo, based on the estimates and all the possible regression for the selection of variable and significance test for 231,382 heads that underwent the carcass measurements. The average and standard deviation for the live weight, carcass weight, dressing percentage, eye muscle area, backfat thickness, and marbling score were 654.79${\pm}$91.61 kg, 362.30${\pm}$67.15 kg, 59.52${\pm}$0.03%, 81.79${\pm}$12.21 $cm^2$, 11.39${\pm}$5.40 mm, 4.38${\pm}$2.29, respectively. The live weight, carcass weight, dressing percentage, eye muscle area, backfat thickness, and marbling score for cow were 532.79${\pm}$78.38 kg, 313.40${\pm}$44.90 kg, 56.50${\pm}$0.03%, 75.24${\pm}$10.69 $cm^2$, 11.82${\pm}$5.10 mm, 4.30${\pm}$2.06, respectively, while for bull were 619.74${\pm}$93.27 kg, 376.89${\pm}$48.62 kg, 58.61${\pm}$0.02%, 85.61${\pm}$10.46 $cm^2$, 5.64${\pm}$2.71 mm, 1.41${\pm}$0.83, respectively, and for steer were 681.78${\pm}$70.72 kg, 415.23${\pm}$49.43 kg, 60.19${\pm}$0.02%, 88.29${\pm}$10.27 $cm^2$, 12.71${\pm}$5.23 mm, 5.42${\pm}$1.99, respectively. In the environmental variables selection based on the variables selection method, the examination by carcass traits suggested that the most appropriate model could be determined when five variables were selected for the live weight, carcass weight, dressing percentage, eye muscle area, and four variables for backfat thickness, and marbling score. When they were considered at a time altogether based on multiple traits, it was deemed to be desirable to insert all five variables into the variables for analysis. In addition, high significance was found by carcass traits.

Analysis of Daily Milking Flow in Holstein Dairy Cow Using the LactoCorder (전자식유량계를 활용한 홀스타인 젖소의 비유형질 분석)

  • Cho, Kwang-Hyun;Choi, Jun-Pyo;You, Byung-Wha;Lee, Deuk-Hwan;Kong, Hong-Sik;Park, Kyung-Do;Lee, Hak-Kyo
    • Journal of Animal Science and Technology
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    • v.51 no.4
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    • pp.265-272
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    • 2009
  • A total of 486 milk records were collected from 16 diary farms in Imsil-gun, Jeollabuk-do. Results obtained were as follows: The average 3MG (amount of milk within the first three minute) was 7.44 kg and 55% of total milk yield was produced within 3 min. The average of SPL (% of foam in milk) was 33.93% and the average of MNG (strip yield) was 0.14 kg, which was less than 1% of total milk yield. The averages of HMF (highest milk flow), HMG (maximum milk flow rate in one minute) and DMHG (average milk flow in the main milking phase) were 3.03 kg/min, 2.94 kg/min and 2.05 kg/min, respectively and the average milking speed in Imsil-gun was slower than other regions. The average of tS500(time to reach 0.5 kg/min at beginning) was 0.23min (about 14 seconds) and that of tMGG (duration of the total milking) was 7.75min. The average tMBG (duration of the dry milking phase) was 0.58 min (35 seconds) and that of tMNG (duration of the stripping phase) was 0.42min (14 seconds). The averages of ELHMF (electrical conductivity at highest milk flow) and ELAP (beginning peak level of the electrical conductivity) were 6.81 mS/cm and 7.58 mS/cm, respectively. The average of ELMAX (maximum electrical conductivity) was 7.48 mS/cm and that of ELAD (beginning peak difference of the electrical conductivity) was 0.61 mS/cm. While the total milk yields for DMHG, tMHG (duration of the main milking phase), tPL (duration of the plateau phase), tAB (duration of the descending phase) and tMGG were positively correlated (0.35~0.54), those for tMBG and SPL were negatively correlated (-0.11 and -0.27). As the DMHG increased, tMHG, tPL, tAB, tMGG and SPL decreased. While the cows with higher electrical conductivity at the beginning of milking had less somatic cell counts, cows with higher electrical conductivity after the peak of milk yield had more somatic cell counts. The results of this experiment indicated that through milking based on milking and lactating standards and the regular checking of milking status, the qualities of milk and milk yields could be improved.

Sex Detection and In Vitro Development of Biopsied Bovine Embryo for LAMP Based Embryo Sexing (LAMP 방법에 의한 소 수정란의 성 판별과 Biopsy에 따른 수정란의 체외발달)

  • Cho S. R.;Choi S. H.;Kim H. J.;Han M. H.;Choe C. Y.;Chung Y. G.;Son D. S.
    • Journal of Embryo Transfer
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    • v.20 no.2
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    • pp.169-176
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    • 2005
  • Loop-mediated isothermal amplification (LAMP) is novel DNA amplification methods that amplifies a target sequence specifically under isothemal condition. The present study was to assess the in vitro viability afier biopsy and sexing rate of different types of embryo biopsied. In vivo compact morulae and blastocyst embryos were obtained from Korean Native Cow (KNC) superovulated with FSH (Antorin, R-10) on 7 Day after artificial insemination. in vitro compact morulae and blastocyst embryos were obtained with KNC or Holsteins that were gained on 6, 7 or 8 day after in vitro fertilization(IVF) with frozen semen. Biopsy of bovine embryo was carried out in a $80{\mu}l$ drop with $Ca^{2+}-Mg^{2+}$ free D-PBS and the viability of biopsied embryos were evaluated in IVMD (IFP, Japan) medium at 12 hrs culture time. The sex ratio of biopsied Hanwoo embryos were male vs. female of $43.5\%\;vs.\;56.5\%$ in vivo and $33.9\%\;vs.\;49.2\%$ in vitro respectively, and male rate of biopsied Holstein embryos were significantly higher than female $(70.8\;vs.\;29.2\%)$. and indefinite rate of in vitro embryos was $16.9\%$ and in vivo was not. The degeneration rate of biopsied embryo, in vitro embryos were significantly higher than in vivo $(13.2\%\;vs,\;0.0\%,\;p<0.05)$. The survivability of in vivo embryo were between biopsied following punching method was significantly (P<0.05) higher than bisection method produced embryos $(100\%\;vs.\;83.3\%)$ and in vitro had no difference. However, the degeneration rate of biopsied embryo by bisection method was significantly higher than punching methods between in vivo and in vitro $(16.7\;vs.\;22.6\%,\;respectively,\;p<0.05)$. In conclusion, these results indicate that punching method was optimal and survivability after embryo biopsy was useful for reducing the damage caused by the embryo biopsy procedure for LAMP-based embryo sexing.

Effects of Dietary Herbaceous Peat on In Vitro Fermentation and Milk Production in Dairy Cows (허브부식토의 사료내 첨가에 따른 In Vitro 발효특성과 젖소의 유생산성에 미치는 영향)

  • Kim, Hyeon-Shup;Park, Joong-Kook;Kim, Hong-Yun;Kim, Sang-Bum;Yang, Seung-Hak;Kim, Chang-Hyun;Ahn, Jong-Ho
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.31 no.2
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    • pp.177-190
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    • 2011
  • This study was conducted to determine effects of dietary herbaceous peat on in vitro fermentation and milk production in dairy cows. Ruminal pH, gas production, VFA (volatile fatty acid), Ammonia-N, and rumen degradability were examined by the addition of three times over 0, 1, and 5% herbaceous peat with substrate of timothy hay, and the change of rumen fermentation characteristics were evaluated. In 0, 3, 12 and 24 hours cultivation, all treatments did not show a significant difference but the control at 6 hours appeared significantly lower pH compared to 1 and 5% treatments (p<0.05). The gas production of the treatments significantly increased until 12 hours of cultivation compared to control (p<0.05), the rumen ammonia concentration showed a tendency to increase until 24 hours in all treatment groups, and there was no significant difference between treatments. About the rumen degradability, 5% treatment showed higher rumen degradability in all hours than control and 1% treatment (p<0.05). Meanwhile, for in vivo trial, 16 heads of Holstein lactation dairy cows were selected for experiment for four weeks in order to research the change of milk yield, milk compositions and change of somatic cell counts of lactation dairy cows by herbaceous peat feeding. The milk yield of vitamin C and herbaceous peat treatments (T3) was 25.0 kg but the control was 23.2 kg, herbaceous peat treatment (T1) was 23.1 kg, and vitamin C treatment (T2) was 23.4 kg, so there was linear increase effect of milk yield by T3. The partial significance of the milk (fat, milk protein, lactose, MUN and SNF) and change of somatic cell count before and after experiment by the control and treatments about change of milk and somatic cell counts (p<0.05) were recognized. About change of milk in the first half (1~2 weeks) and latter half (3~4 weeks) during four weeks of experiments period, the herbaceous peat supplement treatments showed a tendency of significant decrease of quality of milk protein and SNF. The control and treatments did not show significant change of blood nutrients (total protein, cholesterol, NEFA, BUN), liver function component (AST, GGT) and minerals (Ca, P, Mg) before and after experiment. In summary, it is judged that herbaceous peat feeding for lactation dairy cows would be recommendable based on the results of milk, somatic cell count physiologically.

Application for Identification of Food Raw Materials by PCR using Universal Primer (일반 프라이머를 이용한 PCR의 식품원료 진위 판별에 적용)

  • Park, Yong-Chjun;Jin, Sang-Ook;Lim, Ji-Young;Kim, Kyu-Heon;Lee, Jae-Hwang;Cho, Tae-Yong;Lee, Hwa-Jung;Han, Sang-Bae;Lee, Sang-Jae;Lee, Kwang-Ho;Yoon, Hae-Seong
    • Journal of Food Hygiene and Safety
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    • v.27 no.3
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    • pp.317-324
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    • 2012
  • In order to determine an authenticity of food ingredient, we used DNA barcode method by universal primers. For identification of animal food ingredients, LCO1490/HCO2198 and VF2/FISH R2 designed for amplifying cytochrome c oxidase subunit1 (CO1) region and L14724/H15915 for cytochrome b (cyt b) region on mitochondrial DNA were used. Livestock (cow, pig, goat, sheep, a horse and deer) was amplified by LCO1490/HCO 2198, VF2/FISH R2 and L14724/H15915 primers. Poultry (chicken, duck, turkey and ostrich) was amplified by LCO1490/HCO 2198 and VF2/FISH R2 primers. But, Fishes (walleye pollack, herring, codfish, blue codfish, trout, tuna and rockfish) were only amplified by VF2/FISH R2 primers. For plant food ingredients, 3 types of primers (trnH/psbA, rpoB 1F/4R and rbcL 1F/724R) have been used an intergenic spacer, a RNA polymerase beta subunit and a ribulose bisphosphate carboxylase region on plastid, respectively. Garlic, onion, radish, green tea and spinach were amplified by trnH/psbA, rpoB 1F/4R and rbcL 1F/724R. The PCR product sizes were same by rpoB 1F/4R and rbcL 1F/724R but, the PCR product size using trnH/psbA primer was different with others for plants each. We established PCR condition and universal primer selection for 17 item's raw materials for foods and determine base sequences aim to PCR products in this study. This study can apply to determine an authenticity of foods through making an comparison between databases and base sequences in gene bank. Therefore, DNA barcode method using universal primers can be a useful for species identification techniques not only raw materials but also processed foods that are difficult to analyze by chemical analysis.