• Mass Spectrometry Letters
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• v.8 no.4
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• pp.109-113
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• 2017

Single analytical procedure including extraction, liquid chromatography, and mass spectrometric analysis was evaluated for the simultaneous measurement of lysophospholipids (LPLs). LPLs, particularly, lysophosphatidic acids (LPA) and sphingosine 1-phosphate (S1P) are lipid messengers ubiquitously found in various biological matrix. The molecular species mediate important physiological roles in association with many diseases (e.g. cancer, inflammation, and neurodegenerative disease), which emphasize the significance of the simple and reliable analytical method for biomarker discovery and molecular mechanistic understanding. Thus, we developed analytical method mainly focusing on, but not limited by those lipid species S1P and LPA using reverse phase liquid chromatography-tandem mass spectrometry (RPLC-ESI-MS-MS). Extraction method was modified based on Folch method with optimally minimal level of ionization additive (ammonium formate 10 mM and formic acid). Reverse-phase liquid-chromatography was applied for chromatographical separation in combination with negative ionization mode electrospray-coupled Orbitrap mass spectrometry. The method validation was performed on human blood plasma in a non-targeted lipid profiling manner with full-scan MS mode and data-dependent MS/MS. The proposed method presented good inter-assay precision for primary targets, S1P and LPA. Subsequent analysis of other types of LPLs identified a broad range of lysophosphatidylcholines (LPCs) and lysophosphatidyl-ethanolamines (LPEs).

• Journal of Ginseng Research
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• v.44 no.1
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• pp.105-114
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• 2020

Background: Exploring the pharmacokinetic (PK) changes of various active components of single herbs and their combinations is necessary to elucidate the compatibility mechanism. However, the lack of chemical standards and low concentrations of multiple active ingredients in the biological matrix restrict PK studies. Methods: A putative multiple reaction monitoring strategy based on liquid chromatography coupled with mass spectrometry (LC-MS) was developed to extend the PK scopes of quantification without resorting to the use of chemical standards. First, the compounds studied, including components with available reference standard (ARS) and components lacking reference standard (LRS), were preclassified to several groups according to their chemical structures. Herb decoctions were then subjected to ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry analysis with appropriate collision energy (CE) in MS² mode. Finally, multiple reaction monitoring transitions transformed from MS² of ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry were used for ultrahigh-performance liquid chromatography coupled with triple quadrupole mass spectrometry to obtain the mass responses of LRS components. LRS components quantification was further performed by developing an assistive group-dependent semiquantitative method. Results: The developed method was exemplified by the comparative PK process of single herbs Radix Ginseng (RG), Radix Polygala (RP), and their combinations (RG-RP). Significant changes in PK parameters were observed before and after combination. Conclusion: Results indicated that Traditional Chinese Medicine combinations can produce synergistic effects and diminish possible toxic effects, thereby reflecting the advantages of compatibility. The proposed strategy can solve the quantitative problem of LRS and extend the scopes of PK studies.

• Korean Journal of Veterinary Service
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• v.28 no.1
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• pp.81-89
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• 2005

Recently, mass spectrometry coupled with liquid chromatography (LC/MS) has been a preferred technique for determination of organic compounds in complex matrixes. LC/MS provides a high degree sensitivity and specificity of the compounds of interest. The purpose of this study was to confirm analytical method of residual 6 benzimidazoles (thiabendazole, oxfendazole, mebendazole, albendazole, flubendazole and fenbendazole) in meat by LC/MS. Benzimidazoles were analyzed by LC/MS on XTerra $C_{18}$ column with 0.01% trifluoroacetic acid-acetonitrile (TFA) in a gradient mode as mobile phase, and that were identified by electrospray ionization with selected ion recording mode at 150-350 amu mass range. Residual benzimidazoles were extracted from tissue with ethylacetate, and elute benzimidazoles with $50\%$ acetonitrile. In the LC/MS analysis of benzimidazoles, signal to noise ratio was showed relatively high in the positive mode and special ion in the quality analysis was determined via $[M+H]^+$ and Fragment ions. A spectrum of benzimidazoles was showed from all 6 benzimidazoles

• Journal of Astronomy and Space Sciences
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• v.16 no.2
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• pp.241-254
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• 1999

Differential time-series observations of BL Camelopardalis classified as a double mode SX Phoenicis type variable were secured with a charge coupled device. The observed photometric data was reduced using the IRAF Package and the differential magnitudes were obtained through aperture photometry. The periods of BL Cam were analyzed with the Generalized Least-Square Method by Vanicek (1971) and the Fourier Decomposition Method. It was found that the first and second period of BL Cam were 0.0391 day respectively which lead the period ratio of P1/P0=0.81. This period ratio is much different from 0.78 determined by other investigators and also much more larger than that of other double-mode SX Phe type variables. In addition, this period ratio is much different from the value expected from the relation between the metallicity and period ratio. From these results, it can be confirmed that BL Cam is the most extreme case among all double-mode SX Phe type variables.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.7
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• pp.16-23
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• 2018

Chaos is one of the most significant topics in nonlinear science, and has been intensively studied since the Lorenz system was introduced. One characteristic of a chaotic system is that the signals produced by it do not synchronize with any other system. It therefore seems impossible for two chaotic systems to synchronize with each other, but if the two systems exchange information in just the right way, they can synchronize. This paper addresses the problem of synchronization in a modified Lorenz chaotic system based on active control, sliding mode control, and the Lyapunov stability theory. The considered synchronization scheme consists of identical drive and response generalized systems coupled with linear state error variables. For this, a brief overview of the modified Lorenz chaotic system is given. Then, control rules are derived for chaos synchronization via active control and slide mode control theory, with a strategy for solving the chattering problem. The asymptotic stability of the overall feedback system is established using the Lyapunov stability theory. A set of computer simulation works is presented graphically to confirm the validity of the proposed method.

• Coupled systems mechanics
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• v.2 no.4
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• pp.389-410
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• 2013

Three-dimensional Lagrangian fluid finite element is applied to seismic response analysis of an oil storage tank with a floating roof. The fluid element utilized in the present analysis is formulated based on the displacement finite element method considering only volumetric elasticity and its element stiffness matrix is derived by using one-point integration method in order to avoid volumetric locking. The method usually adds a rotational penalty stiffness to satisfy the irrotational condition for fluid motion and modifies element mass matrices through the projected mass method to suppress spurious hourglass-mode appeared in compensation for one-point integration. In the fluid element utilized in the present paper, a small hourglass stiffness is employed. The fluid and structure domains for the objective oil storage tank are modeled by eight-node solid elements and four-node shell elements, respectively, and the transient response of the floating roof structure or the free surface are evaluated by implicit direct time integration method. The results of seismic response analyses are compared with those by other method and the validation of the present analysis using three-dimensional Lagrangian fluid finite elements is shown.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.31 no.3 s.258
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• pp.331-337
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• 2007

Rotating cantilever beams can be found in several practical engineering applications such as turbine blades and helicopter rotor blades. For reliable and economic design, it is necessary to estimate the dynamic characteristics of those structures accurately and efficiently since significant variation of dynamic characteristics resulted from rotational motion of the structures. Recently, Differential Transformation Method(DTM) was proposed by Zhou. This method has been applied to fluid dynamics and vibration problems, and has shown accuracy, efficiency and convenience in solving differential equations. The purpose of this study, the free vibration analysis of a rotating cantilever beam, is to seek for the reliable property of DTM and confidence in the results obtained by this method by comparing the results with that of finite element method applied to linear partial differential equations. In particular, this study is worked by supposing optional T-function values because the equations governing chordwise motion are based on two differential equations coupled with each other. This study also shows mode shapes of rotating cantilever beams for various rotating speeds.

• Tribology and Lubricants
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• v.36 no.5
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• pp.290-296
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• 2020

When two layers of carbon nanotube (CNT) arrays are loaded to mate, the free ends of individual CNTs come into contact at the interface of the two layers. This leads to a higher contact resistance due to a smaller contact region. However, when the free CNT ends of one array penetrate into the mating array, the contact region increases, effectively lowering the contact resistance. To explore the penetration of mating CNTs, we perform molecular dynamic simulations of a simple unit cell model, incorporating four CNTs in the lower array layer coupled with a single moving CNT on the upper layer. The interaction with neighboring CNTs is modelled by long-range carbon bond order potential (LCBOP I). The model structure is optimized by energy minimization through the conjugate gradient method. A NVT ensemble is used for maintain a room temperature during simulation. The time integration is performed through the velocity-Verlet algorithm. A significant vibrational motion of CNTs is captured when penetration is not available, resulting in a specific vibration mode with a high frequency. Due to this vibrational behavior, the random behaviors of CNT motion for predicting the penetration are confirmed under the specific gap distances between CNTs. Thus, the probability of penetration is examined according to the gap distance between CNTs in the lower array and the aspect ratio of CNTs. The penetration is significantly affected by the vibration mode due to the van der Waals forces between CNTs.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.13 no.4
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• pp.427-436
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• 2000

This study presents the nonlinear responses of a hinged-clamped beam under broadband random excitation. By using Galerkin's method the governing equation is reduced to a system or nonautonomous nonlinear ordinary differential equations. The Fokker-Planck equation is used to generate a general first-order differential equation in the joint moments of response coordinates. Gaussian and non-Gaussian closure schemes are used to close the infinite coupled moment equations. The closed equations are then solved for response statistics in terms of system and excitation parameters. The case of two mode interaction is considered in order to compare it with the case of three mode interaction. Monte Carlo simulation is used for numerical verification.

• The Journal of the Acoustical Society of Korea
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• v.34 no.3
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• pp.219-226
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• 2015

In this paper, derivation of the STL (Sound Transmission Loss) of a square plate installed in an impedance tube is discussed using an analytic method. Coupled motion of the plate vibration and acoustic field is considered. Vibration of the plate and pressure field inside the tube are expressed in terms of the infinite series of modal functions. Under the plane wave assumption, it is shown that consideration of the first few modes yields sufficiently accurate results. When the boundary of the plate is clamped, vibration mode is assumed as a multiplication of the beam modes corresponding to the crosswise directions. The natural frequencies of the clamped plate are calculated using the Rayleigh-Ritz method. It is found that the STL shows a dip at the lowest natural frequency of the plate, and increases as the frequency decreases below the natural frequency. Comparison of the result in this paper with the STL obtained by measurements and FE computations in the reference shows an excellent agreement.