• Asian Pacific Journal of Cancer Prevention
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• v.14 no.12
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• pp.7339-7344
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• 2013

To analyze the effects of a new unknown peptide DEF on the growth of tumor cells, a fused polypeptide TAT-DV1-DEF was designed and synthesized. The lung adenocarcinoma cell line GLC-82 treated with TAT-DV1-DEF was analyzed with a cell counting kit 8, and the location of polypeptides in cells was observed under laser confocal microscopy. The efficiency of polypeptide transfection and changes in nuclear morphology were analyzed by flow cytometry and fluorescence microscopy, respectively. Finally, the mechanism of tumor cell growth inhibition was evaluated by Western blotting. We found that TAT-DV1-DEF could significantly inhibit the growth of the lung adenocarcinoma cell line GLC-82, but not the normal human embryonic kidney cell line HEK-293. Polypeptides were found to be mostly localized in the cytoplasm and some mitochondria. The efficiency of polypeptide transfection in the two cell types was approximately 99%. Apoptotic nuclei were observed under fluorescence microscopy upon treatment with polypeptides and DAPI staining. Western blot analyses indicated that the polypeptide inhibition of tumor cell growth was apoptosis dependent. In the present study, we demonstrated that fused polypeptides could induce apoptosis of the lung adenocarcinoma cell line GLC-82, indicating that the new unknown peptide DEF has antitumor effects.

• Korean Journal of Veterinary Research
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• v.1 no.1
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• pp.30-35
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• 1961

Inasmuch as a great variety of methods for the leukocyte differential counting have been described, it may be expected that actual counts obtained considerably vary according with the particular methods applied to the enumeration of differentiated leukocyes. An attempt, therefore, was undertaken to compare the values measured by five authentic methods commonly in use with those obtained by thirteen methods devised by the author. The results seemed to indicate that relatively reliable method is to count, end to end, all leucocytes on fields looted on the central horizontal axis of the film and two adjacent lines parallel to the central line and quartering the film.

• Proceedings of the Korea Concrete Institute Conference
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• 2001.05a
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• pp.333-338
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• 2001

Fractal geometry is a non-Euclidean geometry which has been developed to quantitative analysis irregular or fractional shapes. Fractal dimension of irregular surface has fractal values ranging from 2 to 3 and of irregular line profile has fractal values ranging from 1 to 2. In this paper, quantitative analysis of crack growth patterns during the fracture processing of fiber-reinforced cement composites based on fractal geometry. The fracture behaviors of fiber reinforced mortar beams subjected to three-point loading in flexure. The beams all had a single notch depth, but varing volume fractions of polypropylene, cellulose fibers. The crack growth behaviors, as observed through the image processing system, and the box counting method was used to determine the fractal dimension, Df. The results showed that the linear correlation exists between fractal dimension and fracture energy of the fiber reinforced cement mortar.

• 시스템엔지니어링워크숍
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• s.4
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• pp.182-187
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• 2004

Our research proposes how to, systematically, count function point from initial functional requirements based on natural language. Gradually, Function Point Analysis is used to overcome the limitation of LOC(Line Of Code) for estimating software size. Moreover, it plays an important role in cost management. Function point is derived from initial requirements and is determined by experts who have an education for function point. However, currently there are few researches to cout function point by systematic or automatic rules. Through extending our porposed method, we expect that function point is able to be counted automatically or semi-automatically. This would be our future research

• Journal of Radiation Protection and Research
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• v.26 no.3
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• pp.167-175
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• 2001

Semiconductor diode detectors coated with neutron reactive material are presently under investigation for various uses, such as remote sensing of thermal neutrons, fast neutron counting, and thermal neutron radiography. Theory indicates that single-coated devices can yield thermal neutron efficiencies from 4% to 11 %, which is supported by experimental evidence. Radiation endurance measurements indicate that the devices function well up to a limiting thermal neutron fluence of $10^{13}/cm^2$, beyond which noticeable degradation occurs. Thermal neutron contrast images of step wedges and simple phantoms, taken with dual in-line pixel devices, show promise for thermal neutron imaging detectors.

• Proceedings of the KSME Conference
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• 2003.04a
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• pp.2227-2232
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• 2003

For a reasonable analysis of grade efficiency of cyclone separator, in-line measurements of particle size and particle concentration are needed. The purpose of this study is to define the correlation of measurement data and to measure grade efficiency and pressure drop. The authors adapted that the grade efficiency could be calculated directly from the counted particle numbers. The problem of collection efficiency calculation was different from counting rate of aerosol counters under the same conditions. It is important factor to use the rate of the particle counters to obtain reliable results. Therefore, the authors should use and calculate compensation factor considering sensitivity of aerosol counters, gas velocity and concentration affect higher 30% than originally measured grade efficiency. As the pressure drop and grade efficiency increase, as flow rate increase and then also pressure drop and grade efficiency increase.

• Proceedings of the Korean Society of Marine Engineers Conference
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• 2006.06a
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• pp.279-280
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• 2006

In the present study the flow fields around pitching foils have been visualized by using a Schlieren method with a high speed camera in a wind tunnel at low Reynolds number regions. It has been observed that small vortices are shed discretely from the leading and trailing edge and that they stand in line on the integrated streakline of separation shear layer. By counting vortices in the VTR frames it was clarified that the number of vortex shedding from the leading and trailing edge during one pitching cycle strongly depends on the non-dimensional pitching rate.

• Journal of applied mathematics & informatics
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• v.22 no.1_2
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• pp.339-350
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• 2006

The bifurcation point where a satellite component buds from another component is characterized by the existence of the common tangent line between the two osculating components appearing in the degree-n bifurcation set. We investigate the existence, location and number of bifurcation points for satellite components budding from the main component in the degree-n bifurcation set as well as a parametric boundary equation of the main component of the degree-n bifurcation set. Cusp points are also located on the boundary of the main component. Typical degree-n bifurcation sets and their components are illustrated with some computational results.

• The Transactions of the Korean Institute of Electrical Engineers
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• v.45 no.3
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• pp.432-437
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• 1996

This paper describes a diagnostic technique of power transformers by on-line detection of ultrasonic signals. A trend of partial discharge variation in a real transformer was estimated by counting the number of ultrasonic signals until insulation paper in the point-to-plane electrode is punctured. The number of electrical signals is closely related to the ultrasonic signals generated by partial discharge. The trend of the ultrasonic signal number could easily be distinguished by taking moving average. The insulation failure due to partial discharge in transformers can be predicted based on the trend analysis of ultrasonic signal number caused by partial discharge. (author). 14 refs., 10 figs.

• Journal of Information Technology Applications and Management
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• v.29 no.5
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• pp.13-25
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• 2022

The purpose of this study is to explore the factors that influence the purchase of AI speakers in China. We integrate the Unified Theory of Acceptance and Use of Technology (UTAUT) and Task-technology fit (TTF) model into one model and put forward assumptions. According to the characteristics of AI speakers, we selected 6 independent variables, such as Performance Expectation, Effort Expectation, Social Influence, Facilitating Conditions, Task and Technology-characteristics. The final impact on purchase behavior is evaluated through Task-technology fit and purchase intention. After counting 478 samples, through SPSS22.0 and AMOS analysis, hypotheses have been proved by strong experimental data, except facilitating conditions. These results also imply that improving the technical level of AI speakers and enhancing consumers' purchasing intention are the central line of marketing. Based on this, we put forward several suggestions to marketers, including strengthening the research and development of AI speaker technology, and building a circle of friends of AI speakers.