• 한국식품과학회지
• /
• 제36권3호
• /
• pp.518-521
• /
• 2004

이상계 용매시스템을 이용하여 물질을 고순도로 대량 분리 할 수 있는 기술인 countercurrent chromatography를 이용하여 인삼으로부터 생리황성 성분인 saponin을 대량 분리 농축하였다. 용매 조성별 인삼 saponin의 분배계수에 따른 인삼 saponin 분리에 적합한 용매시스템은 chloroform/methanol/water(40/39/21, v/v/v)으로 결정되었으며 HSCCC의 작동 조건은 chloroform/methanol/water 용매시스템의 하층부를 이동상으로 한 head to tail mode에서 이동상의 유속 5mL/min, 인삼추출물 injection량 $200{\mu}L$, 컬럼회전속도 800 rpm의 조건이 적합한 것으로 판단되었다. 이러한 조건하에서 분리된 인삼saponin의 양은 $550.7{\mu}g$으로 HSCCC에 주입한 인삼 추출물 $200{\mu}L$중에 존재하는 총 saponin의 양 $865.5{\mu}g$에 비교하여 전체 수율은 63.6%로 나타났으며 TLC로 각 분획의 순도를 확인할 수 있었다.

• 한국원자력학회:학술대회논문집
• /
• 한국원자력학회 1996년도 추계학술발표회논문집(1)
• /
• pp.301-306
• /
• 1996

To predict the average liquid level under the condition of the countercurrent stratified two-phase flow in a pipe, an analytical model has been suggested. This is made by introducing the interfacial level gradient into the liquid-phase and the gas-phase momentum equations. The analytical method for the gas-phase pressure drop calculation with f$_i$ $\neq$ f$_G$ has also been described using the liquid level prediction model developed in the present study.

• 설비공학논문집
• /
• 제13권4호
• /
• pp.304-311
• /
• 2001

An experimental study on the countercurrent two-phase flow in narrow rectangular channels has been performed. The void fraction and the pressure gradient were investigated using air and water in 760 mm long, 100 mm wide. vertical test sections with 2, 3 and 5 mm channel gaps. Tests were systematically performed with downward liquid superficial velocities and upward gas velocities covering 0 to 0.08 and 0 to 2.5 m/s ranges. respectively. the experimental results were compared with the previous correlations, which were mainly for round tubes, and the qualitative trends were found to be in good agreements. However the quantitative discrepancies were hardly neglected. as the superficial gas velocities increased, the void fraction increased and the pressure gradient decreased, where the effects of the liquid superficial velocities were infinitesimal. as the gap width of the rectangular channel increased the void fraction and the 2-phase frictional pressure gradient approached those values for the round tubes. Equi-periphery diameter, rather than the hydraulic diameter, seemed to be more effective in the analysis of two-phase flow behavior.

• 설비공학논문집
• /
• 제19권6호
• /
• pp.441-446
• /
• 2007

An experimental study on the countercurrent two-phase flow in narrow rectangular channels has been peformed. Countercurrent flow limitation (CCFL) was investigated using air and water in 760mm long, 100mm wide, vertical test sections with 1 and 3mm channel gaps. Tests were systematically performed with downward liquid superficial velocities and upward gas velocities covering 0 to 0.125 and 0 to 3.5m/s ranges, respectively. As the gap width of rectangular channel increased the CCFL water superficial velocity decreased for the given air superficial velocity. Slight increase of the air superficial velocity resulted in the abrupt decrease of water velocity when $j_g=2{\sim}4m/s$. The critical superficial velocity of air, at which the downward flow of water was no longer allowed, also decreased with the increase of gap width. The experimental results were compared with the previous correlations, which were mainly for round tubes, and the qualitative trends were found to be partially acceptable. However the quantitative discrepancies were hardly neglected. New correlation of CCFL was developed and showed good agreement with the experimental data.

• Nuclear Engineering and Technology
• /
• 제31권6호
• /
• pp.618-630
• /
• 1999

An analytical model that includes the steam condensation effect has been derived and a parametric study has been performed. In addition, a series of experiments were performed and a total of 34 experimental data for the onset of CCFL in nearly horizontal countercurrent two-phase How have been obtained for various flow rates of water. Comparisons of the present CCFL data with slug formation models show that the agreement between the present as well as the existing model and the data is about the same. However, the deviation between the Taitel and Dukler's model predictions and the data is the largest when if j$_{f}$<0.04 m/s. A parametric study of the effect of the steam condensation using the present model shows that, when all local conditions are similar, the model predicted local gas velocities that cause the onset of flooding are slightly lower when condensation occurred. Based on the visual observation and the evaluation of the present work, it has been concluded that the criterion derived for the onset of slug flow can be directly used to predict the onset of inner flooding in nearly horizontal two-phase flow within the experimental ranges of the present work.

• 대한기계학회논문집
• /
• 제12권3호
• /
• pp.599-606
• /
• 1988

본 연구에서는 공기와 물을 사용한 근사수평 반류성층유동에서의 계면파 구조 를 측정하였다. 3차원파 영역내의 유동조건하에서 탐침접촉법을 이용하여 계면파의 액막분포도를 구하였으며 Gauss 모델과 비교분석하였다. 또 이 분포도에서 파의 구 조와 관련된 통계치, 즉 평균액막두께, 파진폭, 파고 교란강도등을 계산하여 유동조건 에 따른 경향을 분석하였다. 본 연구와 병행하여 계면전단응력과 계면마찰계수에 관 한 실험을 수행하였는데 이 결과와 계면파 구조와의 상호연관성도 고찰하였다.

• 대한기계학회논문집
• /
• 제9권2호
• /
• pp.232-239
• /
• 1985

근사수평 이상반류유동에서의 플러딩천이에 대한 실험을 수행하였으며 이것을 바탕으로 반류유 동도(flow-regime map)를 완성하였다. 또 플러딩천이에 대한 응축의 영향을 고찰하였는데 플러 딩이 액체입구에서 야기될 때 플러딩 속도는 응축량을 고려한 유효증기량으로 표시되며 이 경우 반드시 히스테리시스효과를 동반하게 된다. 이 효과는 응축에 기인하는 것으로 그 메카니즘을 구명하였다. 또 전달액체유량이 영이 될 때의 임계증기속도는 액체분출유량이나 액체서브쿠울 링의 정도에 무관하며 본 연구에서 사용한 관의 경우, 수정 Wallis 변수로 1.74로 나타났다.

• Natural Product Sciences
• /
• 제21권1호
• /
• pp.30-33
• /
• 2015

Anthocyanins are water soluble plant pigments which are responsible for the blue, red, pink, violet colors in several plant organs such as flowers, fruits, leaves and roots. In recent years, anthocyanin-rich foods have been favored as dietary supplements and health care products due to diverse biological activities of anthocyanins including antioxidant, anti-allergic, anti-diabetic, anti-microbial, anti-cancer and preventing cardiovascular disease. High-performance countercurrent chromatography (HPCCC) coupled with reversed-phase medium pressure liquid chromatography (RP MPLC) method was applied for the rapid and efficient isolation of cyanidin 3-glucoside (C3G) and peonidin 3-glucoside (P3G) from black rice (Oryza sativa L., Poaceae). The crude black rice extract (500 mg) was subjected to HPCCC using two-phase solvent system composed of tert-butyl methyl ether/n-butanol/ acetonitrile/0.01% trifluoroacetic acid (TBME/B/A/0.01% TFA, 1 : 3 : 1 : 5, v/v, flow rate - 4.5 mL/min, reversed phase mode) to give enriched anthocyanin extract (37.4 mg), and enriched anthocyanin extract was sequentially chromatographed on RP-MPLC to yield C3G (16.5 mg) and P3G (8.7 mg). The recovery rate and purity of isolated C3G were 76.0% and 98.2%, respectively, and those of P3G were 58.3% and 96.3%, respectively. The present study indicates that HPCCC coupled with RP-MPLC method is more rapid and efficient than multi-step conventional column chromatography for the separation of anthocyanins.

• 한국원자력학회:학술대회논문집
• /
• 한국원자력학회 1995년도 추계학술발표회논문집(1)
• /
• pp.383-388
• /
• 1995

Two-phase transient phenomena in the noncondensable gas-filled closed loop was investigated numerically using the RELAP5/MOD3 version 3.1 computer code. The condensation heat transfer correlation for noncondensable gases was studied in detail. Two modes of the reflux condensation which can be characterized by countercurrent flow of steam and its condensed water and the oscillatory between reflux condensation and natural circulation were predicted well. However, the natural circulation mode which the condensed water carried over the U-bend concurrently with steam was failed to predict.

• 대한기계학회논문집
• /
• 제15권1호
• /
• pp.299-307
• /
• 1991

본 연구에서는 근사수평 반류 성층유동에서의 계면마찰계수에 관한 일반화된 실험식을 개발하고자 한다. 이 실험식은 본 저자가 발표한 포화수-수증기와 물-공기 의 실험자료를 기초로 개발되며 공학적인 응용을 위하여 기액상레이놀즈 수와 유체의 물성치를 포함하는 거시적인 유동변수로 표현된다. 또 동일한 계면 마찰계숭에 대한 Nikuradse의 표면조도와 계면의 특성치로 표현되는 무차원 계면 유효조도와의 상관관 계를 구명하고 포화수-수증기와 물-공기의 2상유동에 공통적으로 적용할 수 있는 무차 원 계면유효조도와의 상관관계를 구명하고 포화수-수증기와 물-공기의 2상유동에 공통 적으로 적용할 수 있는 무차원 계면유효조도를 제안할 예정이다. 마지막으로 본 연 구에서 개발한 실험식과 기존 실험식을 비교 검토하고자 한다.