• 제목/요약/키워드: Costal cartilage

검색결과 48건 처리시간 0.022초

토끼 성장판 연골세포 배양과 자가 이식편에서의 형태학적인 변화 (Chondrocyte Culture from Epiphyseal Plate and its Morphological Changes in Autologous Implants of Rabbit)

  • 양영철;정해일;최장석
    • 생명과학회지
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    • 제10권4호
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    • pp.408-421
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    • 2000
  • We tried to establish the culture method of the chondrocyte isolated from the epiphyseal cartilage and to investigate morphological changes of chondrocyte cultured with enzyme-digested costal cartilage, the perichondrium and experimentally damaged meniscus of rabbit. De novo chondrocyte pellets were prepared from epiphyseal plates by culturing isolated epiphyseal chondrocytes from 4 week. old rabbits. We morphologically assessed the cartilage formation of the chondrocyte culture with enzyme-digested costal carilage, the perichondrial culture, the cultured chondrocytes transplants into experimentally damaged meniscus of rabbits, the perichondrial culture, the cultured chondrocytes transplants into experimentally damaged meniscus of rabbit. In the 24 days, the epiphyseal chondrocytes maintained the typical phenotypes of the partial nodular cell formation. The 30 days cryopreserved chondrocytes showed abnormal and irregular shape. In the type II collagen added culture, the chondrocytes showed expanded rough endoplasmic reticulum and small and large round-like vesicles of processes. In the type IV collagen added culture, the chondrocytes showed large perinuclear vaculoes and abundant well-developed rough endoplasmic reticulum of processes. In the culture with enzyme- digested costal cartilage and the perichondrial culture, the chondrocytes showed a few swelling rough endoplasmic reticulum and vacuoles. The cultured epiphyseal chondrocytes maintained typical phenotype and the chondrocytes were grown faster and maintained more typical phenotype in the type II and IV collagen added culture. The transformed chondrocytes secreted abundant extracellular matrix in the type II collagen added culture, and showed processes in the type IV collagen added culture. The perichondrial chondrocytes were grown faster and their implants were able to transplant. The cultured chondrocytes transplanted into experimentally damaged meniscus were adapted between the meniscus tissues. And the immunocyto-chemical reaction of the type II collagen of the chondrocytes were found to be maintained. The chondrocytes cultured cartilage. The chondrocytes secreted abundantly. The cultured chondrocytes transplanted into experimentally damaged meniscus changed immature cells into enlarged mature cells with extracellular secretion.

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선천성 기관 협착환자에서 늑연골 절편을 이용한 기관성형술 1례 (Tracheoplasty with Rib Cartilage Flap for Congenital Tracheal Stenosis -A Case Report-)

  • 이형민
    • Journal of Chest Surgery
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    • 제27권5호
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    • pp.407-412
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    • 1994
  • Congenital long-segment tracheal stenosis which involves nearly entire trachea and carina is very rare disease, but leads to life threatening obstruction in infancy and childhood. Symptoms are ranged from stridor and wheezing to severe cyanosis and respiratory failure. Routine chest X-ray is somewhat helpful to diagnose it, but definitive diagnosis can be made by bronchoscopy or tracheogram for severely narrowed tracheal lumen.Recently, we experienced a case of congenital tracheal stenois, type 1 by Cantrell classification with carinal involvement. After costal cartilage was designed as oval shaped flap and covered with pericardium, anterior and posterior augmentation was done with prepared costal cartilage.This patient died of respiratory failure at 13 days postoperatively, probably due to sustaining obstruction in association in with failure to make a sufficient widening at carinal level.Important issues in the management of congenital tracheal stenosis are rapid diagnosis, selection of appropriate surgical procedure, and detailed anesthetic schedule.In the future, more biocompatible material and more effective surgical procedures should be studied to reduce the surgical mortality and morbidity of the complicated tracheal stenosis.

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자가늑골연골이식을 이용한 제 5중족족지관절재건술 (5th MTP Joint Reconstruction with Autogenic Costal Osteochondral Graft)

  • 박재용;강화준
    • 대한족부족관절학회지
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    • 제17권2호
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    • pp.154-160
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    • 2013
  • The treatment of open, traumatic intraarticular injuries to the metatarsophalangeal joint with severe articular comminution and cartilage defect of metatarsal head is a challenge to the foot surgeon. We report the joint reconstruction treating the injured joint by autogenic costal osteochondral graft with satisfactory outcome.

탈미네랄화된 골분/PLGA 지지체에서 늑연골 세포의 부착과 성장에 미치는 영향 (Effects of Demineralized Bone Particle Loaded Poly(lactic-co-glycolic acid) Scaffolds on the Attachment and Proliferation of Costal Cartilage Cells)

  • 조선아;송정은;김경희;고현아;이동원;권순용;정진화;강길선
    • 폴리머
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    • 제37권5호
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    • pp.632-637
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    • 2013
  • 늑연골세포는 초기 증식능력이 관절연골세포보다 우수하며, 또한 세포 채취에 용이하다는 장점이 있어, 관절연골의 대안으로 사용된다. FDA의 승인을 받은 합성고분자인 폴리락산-글리콜산 공중합체(PLGA)는 조직공학적 생체재료로 사용되고 있으나, 세포 부착률이 낮고, 염증반응을 야기시킨다고 보고되고 있다. 본 연구에서는 세포의 증식에 영향을 주며 염증반응이 감소된다고 보고된 탈미네랄골분(DBP)을 이용하여, 함량별 DBP/PLGA를 제조한 뒤, 세포를 파종하여 연구를 실시하였다. 세포의 부착 및 증식률을 측정하기 위하여 세포 파종한 후 MTT와 SEM 분석을 수행하였으며, DBP가 세포외 기질 형성에 미치는 영향을 확인하고자 글리코스아미노글라이칸(sGAG)과 콜라겐 함량을 측정하였다. 생체 내 외 환경에서 세포의 부착과 증식에 미치는 영향을 관찰하기 위해 연구를 진행하여, PLGA 지지체보다 DBP/PLGA 지지체가 세포의 성장과 증식에 영향을 주는 것을 확인하였으며, DBP의 함량이 증가할수록 효과가 좋은 것으로 나타났다.

자가 늑연골과 심낭막을 이용한 기관 및 기관지성형술 -2례 보고- (Bronchoplasty using Autologous Pericardium and Costal Cartilage -2 cases report-)

  • 권종범;나석주
    • Journal of Chest Surgery
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    • 제29권2호
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    • pp.231-234
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    • 1996
  • 양성원인에 의한 기관지협착 환자에서 기관지 성형술은 협착부위 이하의 폐기능을 유지해 줌으로써 가장 우수한 수술방법으로 알려져 있다. 본 가톨릭 대학교 의과대학 흉부외과학교실에선 2명의 환자에서 (기관 평활근종, 결핵성 기관지 협착) 자가 늑연골과 심낭막을 이용하여 기관 및 기관지 성형술을 시행하였다. 수술후 경과는 양호 하였으며 술후 6개월에 실시한 기관지 내시경 검사상 기관 및 기관지 성형부위의 특별한 이상은 없었다.

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기관절개술 후 발생한 기관무명동맥루에서 연골막를 포함한 늑연골을 이용한 기관성형술 - 수술치험 1예 - (Tracheoplasty with using the Costal Cartilage, Including the Perichondrium, for Treating a Tracheoinnominate Artery Fistula - Surgical experience of one case -)

  • 조성호;계여곤;김종인;조성래
    • Journal of Chest Surgery
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    • 제40권9호
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    • pp.651-654
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    • 2007
  • 기관무명동맥루는 매우 드물지만 높은 사망률을 보이는 질환으로, 장기간의 기관절개술이나 기관내 삽관후 합병증으로 발생한다. 대량 출혈로 인해 기도폐쇄 및 출혈성 쇼크로 생명의 위협이 초래되기 때문에 조기 진단과 즉각적인 치료가 반드시 필요하다. 후두 협착에 대한 수술을 위해 가능한 한 기관의 보존이 필요한 환자에 발생한 기관무명동맥루 환자에서 연골막을 포함한 늑연골을 이용한 기관성형술을 시행하였기에 보고하는 바이다.

한국인(韓國人)의 각장기조직중(各臟器組織中)의 미량중금속(微量重金屬) 원소분포(元素分布) : 연(鉛) 카드뮴 및 동(銅)의 함량(含量) (A Study on Distribution of Heavy Metals in Normal Korean Tissues: Lead, Cadmium and Copper Contents)

  • 장성길;문병열;정규철
    • Journal of Preventive Medicine and Public Health
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    • 제15권1호
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    • pp.95-110
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    • 1982
  • For the purpose of investigation to identify the quantities of heavy metals contained in the tissues of the Korean people, a series of analyses was conducted with atomic absorption spectrophotometry to measure the amount of lead, cadmium and copper distributed in various tissues, such as brain, liver, kidney, heart, lung, spleen, large intestine, hair, muscle, fat, stomach, costal cartilage, blood and urine, obtained from 30 cadaverous bodies who were believed not exposed to the said heavy metals during their life time either occupationally or therapeutically. 1. Lead content: Inter-individual difference was noted in lead contents in each tissue, vis., the average content of lead in hair was the highest with $14.90{\pm}9.74ppm$. The next was in costal cartilage that contained $5.56{\pm}3.86ppm$. The average contents of lead in liver, kidney and muscle were the lowest in value, showing $1.11{\pm}0.92ppm,\;0.73{\pm}0.48ppm\;and\;0.06{\pm}0.06ppm$, respectively, The lead contents in tissues of children under the age of 10 were significantly lower than those of adults, and the higher values were shown in males than in females in general. The lead contents in most of the tissues such as hair, costal cartilage, kidney, lung, fat, stomach, large intestine, heart, muscle and urine were well correlated with age. 2. Cadmium content: The average content of cadmium in kidney appeared to be the highest of other tissues showing $20.72{\pm}9.82ppm$, and liver came next with the value of $1.17{\pm}0.99ppm$. It was estimated that 83.9% of the total cadmium absorbed into the body was stored in kidney, 4.796 in liver, and the remaining 11.4% was distributed in the rest of the tissues. Cadmium contents in tissues showed difference between both sexes showing higher values in the females than in the males, which was quite contrary to the lead content. Cadmium contents in tissues steadily increased in amount with age, showing a significant correlation with age in all tissues. 3. Copper content: The average content of copper in hair was the highest with $10.36{\pm}2.21ppm$, and liver came next with $6.31{\pm}1.24ppm$. The copper that was absorbed into the body was distributed in each tissue: 29.9% in hair, 18.2% in liver, $5{\sim}11.0%$ in brain, heart and kidney, and $3.0{\sim}3.5%$ in stomach, blood, and lung. The copper contents in tissues of children under the age of 10 showed significantly higher values in liver, kidney, heart and large intestine than those of the adults. The copper contents in brain, costal cartilage and fat were well correlated with age showing the highest correlation coefficient of 0.870 (p<0.01) in brain tissue. There was no difference in copper contents in tissues between both sexes, and the values were, in general, lower than those for Japanese.

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PLGA/피브린 지지체가 늑연골 세포의 부착과 성장에 미치는 영향 (Effects of PLGA/Fibrin Scaffolds on Attachment and Proliferation of Costal Cartilage Cells)

  • 송정은;이유정;이윤미;조선아;장지은;이동원;강길선
    • 폴리머
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    • 제37권2호
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    • pp.141-147
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    • 2013
  • Poly(lactide-co-glycolic acid)(PLGA)는 좋은 기계적 성질과 생분해성으로 약물전달시스템 또는 조직공학적으로 널리 이용되고 있으나 낮은 세포 부착률을 가지고 있어 피브린을 첨가하여 이를 보완하고자 하였다. 본 연구에서 사용된 지지체는 트롬빈과 피브리노겐, 그리고 세포을 혼합시킨 후 PLGA 지지체 위에 도포시켜 제조하였다. 세포의 부착 및 증식률을 측정하고자 PLGA/피브린 지지체에 늑연골 세포를 파종 후 1, 3일 및 7일 후 SEM과 MTT 분석을 통하여 측정하였으며, 세포외기질 형성에 미치는 피브린의 영향을 확인하고자 세포를 파종 후 누드마우스에 이식하여 GAG 및 콜라겐 합성의 효과를 확인하였다. 따라서 본 연구에서는 피브린이 혼합된 PLGA 지지체가 생체 내 외 환경에서 세포의 부착 및 증식에 미치는 영향을 확인하고자 연구를 진행하였다. 그 결과, PLGA/피브린 지지체가 기존의 PLGA 지지체와 비교하여 탁월한 세포 성장률을 나타내는 것으로 확인하였다.

골 재생을 위한 생물학적 유도막 (BIOLOGIGIC MEMBRANE FOR GUIDED BONE REGENERATION)

  • 홍종락;강나라;정필훈
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • 제30권1호
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    • pp.17-24
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    • 2004
  • The purpose of this study was to evaluate the stability and efficacy of biologic membrane made of freeze-dried cartilage as a barrier to facilitate guided bone regeneration in experimental non-healing bone defects in the rat mandible. Nine adult Sprague-Dawley rats (400-500g) were used in experiment. 5.0mm in diameter were created on the mandibular angle area by means of slow-speed trephine drill. In microscopic examination, dynamic immature bone forming at 2 weeks and its calcification at 4 weeks were observed. The membrane made of lyophilized cartilage taken from human costal cartilage seems to be very effective for guided bone regeneration as a biologic membrane and the scaffold for attachment of cells or local drug delivery system of growth factor, which may meet the ideal requirement of a barrier membrane and graft materials.

Onlay Rib Bone Graft in Elevation of Reconstructed Auricle: 17 Years of Experience

  • Kim, Taehoon;Han, Jihyeon;Lee, Yoonho
    • Archives of Plastic Surgery
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    • 제40권3호
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    • pp.209-213
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    • 2013
  • Background A cartilage wedge block and covering flap are standard procedures for firm elevation of the ear in microtia correction. However, using costal cartilage for elevation of the reconstructed auricle can be insufficient, and the fixed cartilage wedge block may be absorbed or may slip out. Furthermore, elevating covering flaps is time-consuming and uses up fascia, a potential source of reconstruction material. Therefore, we propose an innovative method using autologous onlay rib bone graft for auricular elevation of microtia. Methods From February 1995 to August 2012, 77 patients received a first stage operation with a rib cartilage framework graft. In the second stage operation, a small full thickness of rib bone was harvested through the previous donor scar. The bihalved rib bone was inserted into the subperiosteal pocket beneath the cartilage framework. Results The follow-up time ranged from 1 month to 17 years, with a mean of 3 years. All of the patients sustained the elevation of their ears very well during the follow-up period. Donor site problems, except for hypertrophic scars, were not observed. Surgery-related complications, specifically skin necrosis, infection, or hematoma, occurred in 4 cases. Conclusions Onlay rib bone graft used to elevate the reconstructed auricle is a more anatomically appropriate material than cartilage, due to the bone-to-bone contact between the bone graft and the temporal bone. Postoperative minor correction of the elevation degree is straightforward and the skin graft survives better. Therefore, reconstructed auricle elevation using onlay rib bone graft is a useful and valuable method.