Lipase is a well-known and highly in-demand enzyme. During the last decade, several lipase optimization studies have been reported. However, production costs have always been a bottleneck for commercial-scale microbial enzyme production. This research aimed to optimize the conditions for lipase production by Limtongozyma siamensis DMKU-WBL1-3 via a One-Factor-At-a-Time (OFAT) approach combined with statistical methods while using a low-cost substrate. Results suggest that low-cost substrates can be substituted for all media components. An optimal medium was found, using response surface methodology (RSM) and central composite design (CCD), to consist of 0.50% (w/v) sweet whey, 0.40% (w/v) yeast extract (food grade), and 2.50% (v/v) palm oil with the medium pH adjusted to 4 under shaking flask cultivation. From an economic point of view, this work was successful in reducing production costs while increasing lipase productivity. The medium costs were reduced by 87.5% of the original cost while lipase activity was increased by nearly 6-fold. Moreover, lipase production was further studied in a 2-L stirred-tank fermentor. Its activity was 1,055.6 ± 0.0 U/ml when aeration and agitation rates were adjusted to 1 vvm and 170 rpm, respectively. Interestingly, under this optimal lipase production, the yeast showed accumulated lipids inside the cells. The primary fatty acid is a monounsaturated fatty acid (MUFA) that is typically linked to health benefits. This study hence reveals promising lipase production and lipid accumulation by L. siamensis DMKU-WBL1-3 that are worthy of further study.
Partho Sarothi Roy;Young Don Yoo;Suhyun Kim;Chan Seung Park
청정기술
/
제29권1호
/
pp.53-58
/
2023
This study shows the summary of the economic performance of excess electricity conversion to hydrogen as well as methane and returned conversion to electricity using a fuel cell. The methane production process has been examined in a previous study. Here, this study focuses on the conversion of methane to electricity. As a part of this study, capital expenditure (CAPEX) is estimated under various sized plants (0.3, 3, 9, and 30 MW). The study shows a method for economic optimization of electricity generation using a fuel cell. The CAPEX and operating expenditure (OPEX) as well as the feed cost are used to calculate the discounted cash flow. Then the levelized cost of returned electricity (LCORE) is estimated from the discounted cash flow. This study found the LCORE value was ¢10.2/kWh electricity when a 9 MW electricity generating fuel cell was used. A methane production plant size of 1,500 Nm3/hr, a methane production cost of $11.47/mcf, a storage cost of $1/mcf, and a fuel cell efficiency of 54% were used as a baseline. A sensitivity analysis was performed by varying the storage cost, fuel cell efficiency, and excess electricity cost by ±20%, and fuel cell efficiency was found as the most dominating parameter in terms of the LCORE sensitivity. Therefore, for the best cost-performance, fuel cell manufacturing and efficiency need to be carefully evaluated. This study provides a general guideline for cost performance comparison with LCORE.
Rice is not only main food but also key farm income source of Korean farmers. In spite of the above facts, rice productivity was decreased on account of drought in every 2 or 3 years interval owing to the vulnerability of irrigation facilities throughout Korea in the past decades. As an context of the first five year economic development plan, all weather farming programme including 4 big river basin comprehensive development projects and large and medium sized irrigation water development projects were carried out successfully. Therefore the area of irrigated paddy were increased from 58% in 1970 to 76.2% in 1999. In the past decades, the Government had invested heavy financial funds to develop irrigation water but as an factor share analysis, the contribution rates of irrigation water and investment for farmland base development project have not been identified yet in national agricultural economic level. It is very scarce to find out the papers concerned to macro-economic factor share analysis or contribution rates of water and investment cost to rice production value in Korea considering the production function of the quantity of irrigation water and investment cost as independent variables. Accordingly this paper covered and aimed at identifying (1) derivation of rice production function with the time serial data from 1965 to 1999 and the contribution rates of irrigation water and total investment cost for farmland base development project. The analytical model of the contribution rates was adapted the famous Cobb-Douglass production function. According to the model analysis, the contribution rate of irrigation water to rice production in Korea was shown 37.8% which was equivalent to 0.28 of the production elasticity of water. The contribution rate of farmland base development project cost was revealed 22% and direct production cost of rice was contributed 60% in the growth of rice production and farm mechanization costs contributed to 18% of it respectively. The two contribution rates comparing with the direct production cost were small but without irrigation water and farmland base development, application of high-pay off inputs and farm mechanization might be impossible. Considering the food security and to cope with the frequent drought, rice farming and investment for the irrigation water development should be continued even in WTO system.
For the economic analysis of fuel cells, levelized cost of electricity was calculated according to the type, capacity, and annual production of the fuel cells. The cost of every component was calculated through the system component breakdown. The direct cost of the system included stack cost, component cost, assembly, test, and conditioning cost, and profit markup cost were added. The effect of capacity and annual production was analyzed by fuel cell type. Sensitivity analysis was performed according to stack life, capital cost, project period, and fuel cost. As a result, it was derived how much the economic efficiency of the fuel cell improves as the capacity increases and the annual production increases.
This dissertation is assumed to continuously occur adjustment cost on present investment. So, I derived from time-nonseparable production-based CAPM and tested the performance of model through data. I also compared time-nonseparable production-based CAPM with time-separable production-based CAPM and CCAPM, CAPM through testifying the performance of model. At the part of applied application, I estimated time-nonseparable PCAPM-betas. The data of Korea consists of 320 listed companies on Korea Stock Exchange (KOSPI) from first quarter 1987 to first quarter 2002. This data also is categorized by scale and industries. Additionally, I estimated time-nonseparable PCAPM-betas through 500 listed companies of New York Stock Exchange (NYSE) from first quarter 1973 to first quarter 2002. I observed the statistical significance of 230 firms by 320 companies in Korea. After that, I compared time-nonseparable PCAPM-betas by firms with time-separable production-based CAPM-betas and CCAPM-betas, CAPM-betas through individual firms. At empirical test, I found that estimated parameter of adjustment cost on time-nonseparable production-based CAPM by scale and industries in Korea had positive value and statistical significance, Moreover, this approach proved to resolve the underestimation of adjustment cost on time-separable production-based CAPM by scale and industries. I also found that the time-nonseparable PCAPM performed better than time-separable production-based CAPM and CCAPM, CAPM. The result from U.S data proved to have similarity to that of Korea. Specifically, I found that time-nonseparable PCAPM-betas by firms performed better than CAPM-betas on individual firms in Korea.
In this paper, a determination method of number of AGVs fer introducing to the multi-path material handling systems is presented by using genetic algorithm. For serving the raw material to each work stations automatically, there needs to introduce a AGVs for transfer the raw martial. To reduce the overall production cost in the material handling systems, however, a trade off exists between the amount of inventory hold on the shop floor and the number of AGVs needed to provide adequate service. In this paper, firstly a objective function which included the net present fixed costs of each stations and each purchased AGVs, delivering cost. stock inventory cost, and safety stock inventory cost is presented. Secondly by using genetic algorithm, the optimal reorder quantity at each stations is decided, where the number of AGVs is increased step by step. From a simulation with different GA parameters, we can determine a optimal number of AGVs to reduce the overall production cost. Thus, the effectiveness of GA for determining the number of AGVs is verified in automated material handling systems.
In previous studies, a system was developed for classifying items of uninsured cost and for generating factors and formulas by item for calculating accident loss costs. However, the loss cost of stopped production was not considered when the system was being developed. In addition, the system which was developed in previous studies had problems such as input error and data collection, owing to numerous input items. Therefore, this study developed a Revised system which considers the loss cost of stopped production, and a Simple system for improving the problems in input errors and data collection. In this study, unquantifiable factors were not considered. Further study that takes these factors into consideration is necessary.
The definitions of organic farming method has rather philosophical meaning than application of agricultural practices. The meaning of organic farming method has certainly basic differences in comparison with the conventional farming methods. The main definitions of organec farming method have certainly to reduce or not to apply any kinds of farm inputs materials by increasing quality of agricultural prod-ucts. However, the quality of produced food by organic farming method should be improved in compari-son with cinvethional farming method. By applying organic method, the qualith of food, envir-onment as well as spiritual attitude of human being should be improved in future. Actually, there are very wide fields of research on organic farming methods such as production tech-niques, soil and water conservation, plant and animal production, marketing and consumers behavior on organic products. In this article the present situation of organic farming methods in the United States of Americal is investigated in cimparison with conventional rice production. The production cost of rice per acre in the United States of America mde 58, 788 Won in 1986, while it was 121, 699 Won in Korea having about 107 percent higher in Korea than tham the U.S.A There is a larger rice farm cost analysis between conventional and organic rice farm in California. The cash cost pre 1 lbs of conventional rice made 4.86 $, while the organic rice was 6.96$ showing about 43.2%higher level of cost in organic products(1 lbs=0.45359kg) At present, there is less econmic advantage of organic rice production in California because of lower yields as well as price levels, Therefore, the total net return over cash cost peracer/yeat was a little lower in organic rice production than conventional rice prouction.
There are many cases of production processes which intermittently produce several different kinds of products for stock through one set of physical facility. In this case, an important question is what size of production run should be prduced once we do set-up for a product in order to minimize the total cost, that is, the sum of the set-up, carrying, and stock-out costs. This problem is used to be called scheduling of multiple products through a single facility in the production management field. Despite the very common occurrence of this type of production process, no one has yet devised a method for determining the optimal production schedule. The purpose of this study is to develop quantitative analytical models which can be used practically and give us rational production schedules. The study is to show improved models with application to a can-manufacturing plant. In this thesis the economic production quantity (EPQ) model was used as a basic model to develop quantitative analytical models for this scheduling problem and two cases, one with stock-out cost, the other without stock-out cost, were taken into consideration. The first analytical model was developed for the scheduling of products through a single facility. In this model we calculate No, the optimal number of production runs per year, minimizing the total annual cost above all. Next we calculate No$_{i}$ is significantly different from No, some manipulation of the schedule can be made by trial and error in order to try to fit the product into the basic (No schedule either more or less frequently as dictated by) No$_{i}$, But this trial and error schedule is thought of inefficient. The second analytical model was developed by reinterpretation by reinterpretation of the calculating process of the economic production quantity model. In this model we obtained two relationships, one of which is the relationship between optimal number of set-ups for the ith item and optimal total number of set-ups, the other is the relationship between optimal average inventory investment for the ith item and optimal total average inventory investment. From these relationships we can determine how much average inventory investment per year would be required if a rational policy based on m No set-ups per year for m products were followed and, alternatively, how many set-ups per year would be required if a rational policy were followed which required an established total average inventory inventory investment. We also learned the relationship between the number of set-ups and the average inventory investment takes the form of a hyperbola. But, there is no reason to say that the first analytical model is superior to the second analytical model. It can be said that the first model is useful for a basic production schedule. On the other hand, the second model is efficient to get an improved production schedule, in a sense of reducing the total cost. Another merit of the second model is that, unlike the first model where we have to know all the inventory costs for each product, we can obtain an improved production schedule with unknown inventory costs. The application of these quantitative analytical models to PoHang can-manufacturing plants shows this point.int.
The presence of imprecise measurement may seriously affect the efficiency of process control and production cost. A cost model is derived to determine the design parameters of the economic asymmetric $\bar{X}$ and S control charts including measurement errors. The effects of imprecise measurement on the performance of the economic asymmetric $\bar{X}$ and S control charts and production cost are examined for the case where the process mean and process standard deviation may change. Application of the proposed control charts is demonstrated through an example. Numerical examples illustrate the effects of imprecise measurement on the design parameters of the proposed control charts. It shows that the imprecision measurement may seriously affrct the ability of the proposed control charts to detect process disturbances quickly, change the sampling frequency, and increase the production cost compared to the control charts excluding measurement errors.
본 웹사이트에 게시된 이메일 주소가 전자우편 수집 프로그램이나
그 밖의 기술적 장치를 이용하여 무단으로 수집되는 것을 거부하며,
이를 위반시 정보통신망법에 의해 형사 처벌됨을 유념하시기 바랍니다.
[게시일 2004년 10월 1일]
이용약관
제 1 장 총칙
제 1 조 (목적)
이 이용약관은 KoreaScience 홈페이지(이하 “당 사이트”)에서 제공하는 인터넷 서비스(이하 '서비스')의 가입조건 및 이용에 관한 제반 사항과 기타 필요한 사항을 구체적으로 규정함을 목적으로 합니다.
제 2 조 (용어의 정의)
① "이용자"라 함은 당 사이트에 접속하여 이 약관에 따라 당 사이트가 제공하는 서비스를 받는 회원 및 비회원을
말합니다.
② "회원"이라 함은 서비스를 이용하기 위하여 당 사이트에 개인정보를 제공하여 아이디(ID)와 비밀번호를 부여
받은 자를 말합니다.
③ "회원 아이디(ID)"라 함은 회원의 식별 및 서비스 이용을 위하여 자신이 선정한 문자 및 숫자의 조합을
말합니다.
④ "비밀번호(패스워드)"라 함은 회원이 자신의 비밀보호를 위하여 선정한 문자 및 숫자의 조합을 말합니다.
제 3 조 (이용약관의 효력 및 변경)
① 이 약관은 당 사이트에 게시하거나 기타의 방법으로 회원에게 공지함으로써 효력이 발생합니다.
② 당 사이트는 이 약관을 개정할 경우에 적용일자 및 개정사유를 명시하여 현행 약관과 함께 당 사이트의
초기화면에 그 적용일자 7일 이전부터 적용일자 전일까지 공지합니다. 다만, 회원에게 불리하게 약관내용을
변경하는 경우에는 최소한 30일 이상의 사전 유예기간을 두고 공지합니다. 이 경우 당 사이트는 개정 전
내용과 개정 후 내용을 명확하게 비교하여 이용자가 알기 쉽도록 표시합니다.
제 4 조(약관 외 준칙)
① 이 약관은 당 사이트가 제공하는 서비스에 관한 이용안내와 함께 적용됩니다.
② 이 약관에 명시되지 아니한 사항은 관계법령의 규정이 적용됩니다.
제 2 장 이용계약의 체결
제 5 조 (이용계약의 성립 등)
① 이용계약은 이용고객이 당 사이트가 정한 약관에 「동의합니다」를 선택하고, 당 사이트가 정한
온라인신청양식을 작성하여 서비스 이용을 신청한 후, 당 사이트가 이를 승낙함으로써 성립합니다.
② 제1항의 승낙은 당 사이트가 제공하는 과학기술정보검색, 맞춤정보, 서지정보 등 다른 서비스의 이용승낙을
포함합니다.
제 6 조 (회원가입)
서비스를 이용하고자 하는 고객은 당 사이트에서 정한 회원가입양식에 개인정보를 기재하여 가입을 하여야 합니다.
제 7 조 (개인정보의 보호 및 사용)
당 사이트는 관계법령이 정하는 바에 따라 회원 등록정보를 포함한 회원의 개인정보를 보호하기 위해 노력합니다. 회원 개인정보의 보호 및 사용에 대해서는 관련법령 및 당 사이트의 개인정보 보호정책이 적용됩니다.
제 8 조 (이용 신청의 승낙과 제한)
① 당 사이트는 제6조의 규정에 의한 이용신청고객에 대하여 서비스 이용을 승낙합니다.
② 당 사이트는 아래사항에 해당하는 경우에 대해서 승낙하지 아니 합니다.
- 이용계약 신청서의 내용을 허위로 기재한 경우
- 기타 규정한 제반사항을 위반하며 신청하는 경우
제 9 조 (회원 ID 부여 및 변경 등)
① 당 사이트는 이용고객에 대하여 약관에 정하는 바에 따라 자신이 선정한 회원 ID를 부여합니다.
② 회원 ID는 원칙적으로 변경이 불가하며 부득이한 사유로 인하여 변경 하고자 하는 경우에는 해당 ID를
해지하고 재가입해야 합니다.
③ 기타 회원 개인정보 관리 및 변경 등에 관한 사항은 서비스별 안내에 정하는 바에 의합니다.
제 3 장 계약 당사자의 의무
제 10 조 (KISTI의 의무)
① 당 사이트는 이용고객이 희망한 서비스 제공 개시일에 특별한 사정이 없는 한 서비스를 이용할 수 있도록
하여야 합니다.
② 당 사이트는 개인정보 보호를 위해 보안시스템을 구축하며 개인정보 보호정책을 공시하고 준수합니다.
③ 당 사이트는 회원으로부터 제기되는 의견이나 불만이 정당하다고 객관적으로 인정될 경우에는 적절한 절차를
거쳐 즉시 처리하여야 합니다. 다만, 즉시 처리가 곤란한 경우는 회원에게 그 사유와 처리일정을 통보하여야
합니다.
제 11 조 (회원의 의무)
① 이용자는 회원가입 신청 또는 회원정보 변경 시 실명으로 모든 사항을 사실에 근거하여 작성하여야 하며,
허위 또는 타인의 정보를 등록할 경우 일체의 권리를 주장할 수 없습니다.
② 당 사이트가 관계법령 및 개인정보 보호정책에 의거하여 그 책임을 지는 경우를 제외하고 회원에게 부여된
ID의 비밀번호 관리소홀, 부정사용에 의하여 발생하는 모든 결과에 대한 책임은 회원에게 있습니다.
③ 회원은 당 사이트 및 제 3자의 지적 재산권을 침해해서는 안 됩니다.
제 4 장 서비스의 이용
제 12 조 (서비스 이용 시간)
① 서비스 이용은 당 사이트의 업무상 또는 기술상 특별한 지장이 없는 한 연중무휴, 1일 24시간 운영을
원칙으로 합니다. 단, 당 사이트는 시스템 정기점검, 증설 및 교체를 위해 당 사이트가 정한 날이나 시간에
서비스를 일시 중단할 수 있으며, 예정되어 있는 작업으로 인한 서비스 일시중단은 당 사이트 홈페이지를
통해 사전에 공지합니다.
② 당 사이트는 서비스를 특정범위로 분할하여 각 범위별로 이용가능시간을 별도로 지정할 수 있습니다. 다만
이 경우 그 내용을 공지합니다.
제 13 조 (홈페이지 저작권)
① NDSL에서 제공하는 모든 저작물의 저작권은 원저작자에게 있으며, KISTI는 복제/배포/전송권을 확보하고
있습니다.
② NDSL에서 제공하는 콘텐츠를 상업적 및 기타 영리목적으로 복제/배포/전송할 경우 사전에 KISTI의 허락을
받아야 합니다.
③ NDSL에서 제공하는 콘텐츠를 보도, 비평, 교육, 연구 등을 위하여 정당한 범위 안에서 공정한 관행에
합치되게 인용할 수 있습니다.
④ NDSL에서 제공하는 콘텐츠를 무단 복제, 전송, 배포 기타 저작권법에 위반되는 방법으로 이용할 경우
저작권법 제136조에 따라 5년 이하의 징역 또는 5천만 원 이하의 벌금에 처해질 수 있습니다.
제 14 조 (유료서비스)
① 당 사이트 및 협력기관이 정한 유료서비스(원문복사 등)는 별도로 정해진 바에 따르며, 변경사항은 시행 전에
당 사이트 홈페이지를 통하여 회원에게 공지합니다.
② 유료서비스를 이용하려는 회원은 정해진 요금체계에 따라 요금을 납부해야 합니다.
제 5 장 계약 해지 및 이용 제한
제 15 조 (계약 해지)
회원이 이용계약을 해지하고자 하는 때에는 [가입해지] 메뉴를 이용해 직접 해지해야 합니다.
제 16 조 (서비스 이용제한)
① 당 사이트는 회원이 서비스 이용내용에 있어서 본 약관 제 11조 내용을 위반하거나, 다음 각 호에 해당하는
경우 서비스 이용을 제한할 수 있습니다.
- 2년 이상 서비스를 이용한 적이 없는 경우
- 기타 정상적인 서비스 운영에 방해가 될 경우
② 상기 이용제한 규정에 따라 서비스를 이용하는 회원에게 서비스 이용에 대하여 별도 공지 없이 서비스 이용의
일시정지, 이용계약 해지 할 수 있습니다.
제 17 조 (전자우편주소 수집 금지)
회원은 전자우편주소 추출기 등을 이용하여 전자우편주소를 수집 또는 제3자에게 제공할 수 없습니다.
제 6 장 손해배상 및 기타사항
제 18 조 (손해배상)
당 사이트는 무료로 제공되는 서비스와 관련하여 회원에게 어떠한 손해가 발생하더라도 당 사이트가 고의 또는 과실로 인한 손해발생을 제외하고는 이에 대하여 책임을 부담하지 아니합니다.
제 19 조 (관할 법원)
서비스 이용으로 발생한 분쟁에 대해 소송이 제기되는 경우 민사 소송법상의 관할 법원에 제기합니다.
[부 칙]
1. (시행일) 이 약관은 2016년 9월 5일부터 적용되며, 종전 약관은 본 약관으로 대체되며, 개정된 약관의 적용일 이전 가입자도 개정된 약관의 적용을 받습니다.