• Advances in aircraft and spacecraft science
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• v.6 no.6
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• pp.479-495
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• 2019

Periodic cellular core structures included in sandwich panels possess good stiffness while saving weight and only lately their potential to act as passive vibration filters is increasingly being studied. Classical homogeneous honeycombs show poor vibracoustic performance and only by varying certain geometrical features, a shift and/or variation in bandgap frequency range occurs. This work aims to investigate the vibration filtering properties of the AUXHEX "hybrid" core, which is a cellular structure containing cells of different shapes. Numerical simulations are carried out using two different approaches. The first technique used is the harmonic analysis with commercially available software, and the second one, which has been proved to be computationally more efficient, consists in the Wave Finite Element Method (WFEM), which still makes use of finite elements (FEM) packages, but instead of working with large models, it exploits the periodicity of the structure by analysing only the unit cell, thanks to the Floquet-Bloch theorem. Both techniques allow to produce graphs such as frequency response plots (FRF's) and dispersion curves, which are powerful tools used to identify the spectral bandgap signature of the considered structure. The hybrid cellular core pattern AUXHEX is analysed and results are discussed, focusing the investigation on the possible spectral bandgap signature heritage that a hybrid core experiences from their "parents" homogeneous cell cores.

• Journal of Magnetics
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• v.7 no.3
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• pp.101-105
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• 2002

MRAM (Magnetoresistive Random Access Memory) is a promising candidate for a universal memory that meets all application needs with non-volatile, fast operational speed, and low power consumption. The simplest architecture of MRAM cell is a series of MTJ (Magnetic Tunnel Junction) as a data storage part and MOS transistor as a data selection part. This paper is for testing the actual electrical parameters to adopt MRAM technology in the semiconductor based memory device. The discussed topics are an actual integration of MRAM core cell and its properties such as electrical tuning of MOS/MTJ for data sensing and control of magnetic switching for data writing. It will be also tested that limits of the MRAM technology for a high density memory.

• Nuclear Engineering and Technology
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• v.34 no.3
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• pp.250-258
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• 2002

This Paper is to apply the well-established coarse mesh finite difference(CMFD) method to the method of characteristics(MOC) transport calculation as an acceleration scheme. The CMFD problem is first formulated at the pin-cell level with the multi-group structure To solve the cell- based multi-group CMFD problem efficiently, a two-group CMFD formulation is also derived from the multi-group CMFD formulation. The performance of the CMFD acceleration is examined for three test problems with different sizes including a realistic quarter core PWR problem. The CMFD formulation provides a significant reduction in the number of ray tracings and thus only about 9 ray tracing iterations are enough for the realistic problem. In computing time, the CMFD accelerated case is about two or three times faster than the coarse-mesh rebalancing(CMR) accelerated case.

• Journal of Life Science
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• v.16 no.5
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• pp.828-833
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• 2006

Maize (Zea mays L.) pistil cell death and stamen cell arrest are pivotal process on the sex determination, which diverges from bisexual state of floral meristem to unisexual state in staminate or pistillate floret. We investigated the temporal and spatial distribution of cell cycle gene expression during maize sex determination. The positive regulatory genes of cell cycle, cyclin A, cyclin B, cyclin dependent kinase (CDK) and Mad2 were highly expressed in the developing pistil and stamen but the expression was disappeared in the dying pistil and arresting stamens. In contrast, the negative regulatory genes of cell cycle, Wee1 and CDK inhibitor (CKI) were expressed in the arresting stamens in the wild-type ear and tasselseed2 mutant tassel, however, these genes were not detected in dying pistil although the cyclin B gene expression was disappeared. These results suggest that both the pistil cell death and stamen cell arrest process in maize sex determination are involved in cell cycle regulation, but the different expression patterns of negative regulatory cell cycle genes in the arresting stamens and aborting pistils suggest that the two processes may have distinctive modes of action.

• Journal of Animal Reproduction and Biotechnology
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• v.37 no.4
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• pp.239-245
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• 2022

Protein and peptide candidates are screened to apply therapeutic application as a drug. Ensuring that these candidates are delivered and maximized effectiveness is still challenging and a variety of studies are ongoing. As drug delivery system vehicles, cell-penetrating peptide (CPP) can deliver various kinds of cargo into the cell cytosol. In a previous study, we developed Ara27 CPP, which are a zinc knuckle family protein of Arabidopsis, and confirmed internalization in human dermal fibroblasts and human dental pulp stem cells at low concentration with short time treatment condition without any toxicity. Ara27, an amphipathic CPP, could be modified and utilized in the biomedical field excluding the risk of toxicity. Therefore, we would like to confirm the non-toxic induced penetrating ability of Ara27 in various cell lines. The purpose of this study was to screen the cell internalization ability of Ara27 in various cell lines and to confirm Ara27 as a promising core CPP structure. First, Ara27 was screened to confirm non-toxicity concentration. Then, fluorescence-labeled Ara27 was treated on human normal cell lines, cancer cell lines and animal cell lines to identify the cellular internalization of Ara27. Ara27 was well intracellular localized in all cell lines and the intensity of fluorescence was remarkably increased in time pass manner. These results indicate that Ara27 has the potential as a core structure for applications in various drug delivery systems.

• Molecules and Cells
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• v.45 no.12
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• pp.923-934
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• 2022

Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have great potential in applications such as regenerative medicine, cardiac disease modeling, and in vitro drug evaluation. However, hPSC-CMs are immature, which limits their applications. During development, the maturation of CMs is accompanied by a decline in their proliferative capacity. This phenomenon suggests that regulating the cell cycle may facilitate the maturation of hPSC-CMs. Aurora kinases are essential kinases that regulate the cell cycle, the role of which is not well studied in hPSC-CM maturation. Here, we demonstrate that CYC116, an inhibitor of Aurora kinases, significantly promotes the maturation of CMs derived from both human embryonic stem cells (H1 and H9) and iPSCs (induced PSCs) (UC013), resulting in increased expression of genes related to cardiomyocyte function, better organization of the sarcomere, increased sarcomere length, increased number of mitochondria, and enhanced physiological function of the cells. In addition, a number of other Aurora kinase inhibitors have also been found to promote the maturation of hPSC-CMs. Our data suggest that blocking aurora kinase activity and regulating cell cycle progression may promote the maturation of hPSC-CMs.

• Nuclear Engineering and Technology
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• v.14 no.2
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• pp.86-91
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• 1982

In 1977, spent fuel storage capacity of Ko-Ri Unit 1 was raised to contain 4-2/3 core, by reducing the center-to-center spacing between fuel assemblies from 53.34cm to 36cm. In this paper the adequacy is discussed in detail by examining the previous design analysis report. According to the analytic method presented by Core Performance Branch, study on credible abnormal moderator density condition is performed by using KENO-IV for the redesigned spent fuel storage facility. Result shows that 36cm for the center-to-center spacing between fuel assemblies is not enough to keep the storage safe at water density of 0.1143g/㎤, which gives the maximum $K_{eff}$ 0.9958$\pm$0.0048, which exceeds the CPB regulation limit 0.98. From sensitivity study regarding to the center-to-center spacing, it should be maintained to space greater than 43cm in order to meet the CPB requirements.s.

• Journal of Microbiology and Biotechnology
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• v.15 no.3
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• pp.544-550
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• 2005

To develop a highly efficient mammalian expression vector, a series of vectors were constructed based on the murine cytomegalovirus (MCMV) immediate-early (IE) promoter and human ${\beta}$-globin second intron. The resulting MCMV promoter was several-fold stronger than the HCMV promoter in various mammalian cell lines, such as the NIH3T3, Neuro-2a, 293T, and HT1080 cell lines, and was only slightly weaker than the HCMV promoter in HeLa and CHO cells. The inclusion of the human ${\beta}$-globin second intron behind the MCMV promoter or HCMV promoter markedly enhanced the promoter activity in various mammalian cell lines, and the resultant MCMV/Glo-I expression system was stronger than the HCMV promoter from 4.7- to 11.2-fold in every cell line tested. Also, the MCMV/Glo-I promoter induced a higher level of the VSV-G protein in a transiently transfected 293T cell line, which is useful for the production of recombinant retrovirus and lentivirus vectors.

• Proceedings of the Korean Nuclear Society Conference
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• 1997.05a
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• pp.77-82
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• 1997

The CANDU-6 transition core refuelled from 37-element fuel to CANFLEX-NU fuel has been evaluated by an 100full power day time-dependent fuel-management simulation to find the core compatibility with the CANFLEX fuel loading. The simulation calculations for the transition core were carried out with the RFSP code, provided by the cell averaged fuel properties obtained from the POWDERPUFS-V code. The simulation results were compared with those of the current 37-element fuel loading only. The results show that the CANFLEX-NU fuel bundles will be compatible with the CANDU-6 reactor because the core physics characteristics of CANFLEX-NU fuel are very similar to those of the 37-element fuel bundle.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.34 no.4
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• pp.349-354
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• 2010

Fuel cells have attracted enormous interest as new power sources because the cells can be used to solve the problem of environmental pollution as well as the natural-resource exhaustion problem. In this study, hydrogen-gas flow in microchannels of different shapes was numerically analyzed to improve the efficiency of a microfuel cell. Flow characteristics in six microchannels of different shapes but under identical boundary conditions were simulated. The analysis result shows that the flow characteristics such as velocity, uniformity, and flow rate, greatly depend upon the channel shape. This implies that the efficiency of microfuel cell can be expected to be increased by adopting the optimal configuration of channel shape for hydrogen-gas flow. The experimental results show that power density of a PEMFC with a microflow channel is higher than that of a PEMFC without a microflow channel; however, a durable catalyst is required in MEA.