• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2001.06a
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• pp.15-19
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• 2001

Creating an artificial strain with a minimal gene set for a specific purpose is every biologist's dream. With the complete genome sequencing of more than 50 microorganisms and extensive functional analyses of their genes, it is possible to design a genetic blueprint for a simple custom-designed microbe with the minimal gene set. Two different approaches are being considered. The first 'top-down' approach is trimming the genome to a minimal gene set by selectively removing genes of an organism thought to be unnecessary based on microbial genomics. The second 'bottom-up' approach is to synthesize the proposed minimal genome from basic chemical building blocks. The 'top-down' approach starting with the genome of a well known microorganism is more technically feasible, whereas the bottom-up approach may not be attainable in the nearest future because of the lack of the complete functional analysis of the genes needed for a life. Here in this study, we used the top-down approach to minimize the E. coli genome to create an artificial organism with 'core' elements for self-sustaining and self-replicating cells by eliminating unnecessary genes. Using several different kinds of sophisticated deletion techniques combined with a p:1age and transposons, we deleted about 19％ of the E. coli genome without causing any damages to cellular growth. This smaller E. coli genome will be further reduced to a genome with a minimal gene l;et essential for cell life. This minimized E. coli genome can lead to the construction of many custom-designed strains with myriad practical and commercial applications.

• The Korean Journal of Physiology and Pharmacology
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• v.12 no.6
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• pp.337-342
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• 2008

Human bone marrow mesenchymal stem cells (hBM-MSCs) represent a potentially valuable cell type for clinical therapeutic applications. The present study was designed to evaluate the effect of long-term culturing (up to $10^{th}$ passages) of hBM-MSCs from eight individual amyotrophic lateral sclerosis (ALS) patients, focusing on functional ion channels. All hBM-MSCs contain several MSCs markers with no significant differences, whereas the distribution of functional ion channels was shown to be different between cells. Four types of $K^+$ currents, including noise-like $Ca^{+2}$-activated $K^+$ current ($IK_{Ca}$), a transient outward $K^+$ current ($I_{to}$), a delayed rectifier $K^+$ current ($IK_{DR}$), and an inward-rectifier $K^+$ current ($K_{ir}$) were heterogeneously present in these cells, and a TTX-sensitive $Na^+$ current ($I_{Na,TTX}$) was also recorded. In the RT-PCR analysis, Kv1.1,, heag1, Kv4.2, Kir2.1, MaxiK, and hNE-Na were detected. In particular, ($I_{Na,TTX}$) showed a significant passage-dependent increase. This is the first report showing that functional ion channel profiling depend on the cellular passage of hBM-MSCs.

• Proceedings of the Microbiological Society of Korea Conference
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• 2008.05a
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• pp.118-118
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• 2008

The phylogenetic diversity of microbial communities in calcareous mats from Spearfish Creek, a freshwater stream located in the Black Hills of South Dakota, was examined using PCR-based 16S rDNA sequence analysis. In this study, two types of calcareous mats were compared: mature mats formed on the natural substrate of rock surfaces and developing mats on an artificial substrate of glass slides. Among 63 unique isolates from a clone library of 16S rRNA genes from mature mat samples, there were 8 phyla of Bacteria represented. The predominant phylum was Proteobacteria (48%), with the $\beta$ subclass being the largest group. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes from slide samples collected at intervals for four months showed considerable diversity of the microbial community from the earliest stages of community development. Amplicons isolated from DGGE gels and sequenced indicated that community succession has occurred without increasing microbial diversity. However, light microscopic analysis revealed a significant increase in microbial cell density throughout the community development. Scanning electron microscopy of mat samples provides evidence that diatoms are also important members of calcareous mat communities.

• The Korean Journal of Cytopathology
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• v.17 no.1
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• pp.46-50
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• 2006

A micropapillary variant of urothelial carcinoma (MPC) is a distinct entity with an aggressive clinical course. It has a micropapillary configuration resembling that of ovarian papillary serous carcinoma. Its cytologic features have rarely been reported. We report a case of MPC detected by urine cytology. A woman aged 93 years presented with a chief complaint of macroscopic hematuria. Cytology of her voided urine showed clusters of malignant cells in a micropapillary configuration. Each tumor cell had a vacuolated cytoplasm, a high nuclear:cytoplasmic ratio, and irregular hyperchromatic nuclei. An ureteroscopic examination revealed exophytic sessile papillary masses extending from the left lateral wall to the anterolateral wall of the urinary bladder. A transurethral resection of the tumor was carried out. The tumor was characterized by delicate papillae with a thin, well-developed fibrovascular stromal core and numerous secondary micropapillae lined with small cuboidal cells containing uniform low- to intermediate-grade nuclei and occasional intracytoplasmic mucinous inclusions. These tumor cells infiltrated the muscle layers of the bladder, and lymphatic tumor emboli were frequently seen. Recognizing that the presence of MPC components in urinary cytology is important for distinguishing this lesion from low-grade papillary lesions and high-grade urothelial carcinomas can result in early detection and earlier treatment for an improved treatment outcome.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2000.04a
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• pp.150-156
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• 2000

Oligotrophic bacteria isolated from forest soil showed a specific community consisting of various taxonomic groups compared with those in other soil or aquatic habitats. Based on the cell shape, the isolates were divided into four groups: regular rod, curved/spiral rod, irregular rod, and prosthecate bacteria. The cellular fatty acids 60 oligotrophic isolates were analyzed. The 30 fatty acids which were identified or characterized are classified. At the dendrogram based on cellular fatty acid composition, four clusters(I-IV) were separated at a euclidian distance of about 50. Cluster 3 and 4-a strains were containing Q-8, these strains are accommodated in the Proteobacteria gamma and beta subdivision. The chemotaxonomic profiles of the cluster 4-a strains showed good agreement with those of the genus Burkholderia. Cluster 3 was characterized by the presence of branched-chain fatty acids, iso-C15:0, iso-C17:1, and iso-C17:0 as the major components. These chemotaxonomy suggested the close relationship of the isolates with Xathomonas/Sterotrophomonas group. Based on the 16S rDNA sequence analysis, the two representative strains(MH256 and MA828) of cluster 3 showed the close relation to genera, Xathomonas/Sterotrophomonas, but were not included in these genera. These strains were even further away from core Xanthomonas, and clearly were seen to branch outside the cluster formed by the Sterotrophomonas maltophilia. MH256 and MA828 16S rDNA sequence was different enough to put new genus on a separate branch. The isolates with Q-10 were also studied. They are corresponded to the two large groups in Proteobacteria alpha subdivision. One was incorporated in the genus Bradyrhizobium cluster, which also includes Agromonas, a genus for oligotrophic bacteria. The strains of the other group showed high similarity to the genus Agrobacterium.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.30 no.5C
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• pp.371-381
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• 2005

In this paper, we propose an advanced hardware architecture for the CAVLC entropy encoder/decoder engine for real time video compression. The CAVLC (Context-based Adaptive Variable Length Coding) is a lossless compression method in H.264/AVC and it has high compression efficiency but has computational complexity. The reference memory size is optimized using partitioned storing method and memory reuse method which are based on partiality of memory referencing. We choose the hardware architecture which has the most suitable one in several encoder/decoder architectures for the mobile devices and improve its performance using parallel processing. The proposed architecture has been verified by ARM-interfaced emulation board using Altera Excalibur and also synthesized on Samsung 0.18 um CMOS technology. The synthesis result shows that the encoder can process about 300 CIF frames/s at 150MHz and the decoder can process about 250 CIF frames/s at 140Mhz. The hardware architectures are being used as core modules when implementing a complete H.264/AVC video encoder/decoder chip for real-time multimedia application.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.13 no.3
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• pp.288-300
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• 2002

In this paper, a novel method of equalization of RF transceivers is suggested for MIMO(Multiple Input Multiple Output) antenna actively studied for high speed data transmission in the recent IMT-2000 system. The core of suggestion is in equalizing the transfer characteristics of multiple transceivers using feedback and memory during the predefined calibration time. This makes it possible to weight the signals in the intermediate frequency, which is easier in the application of recently developed DoA(Direction of Arrival) algorithms. In addition, the time varying optimum cell formation according to traffic is feasible by antenna beam-forming based on the DoA information. The suggested method of equalizing multiple transceivers are successfully verified using envelope simulation. two outputs. This paper is concerned with the diagnosis of multiple crosstalk-faults in OSM. As the network size becomes larger in these days, the convent.
nal diagnosis methods based on tests and simulation be.
me inefficient, or even more impractical. We propose a simple and easily implementable alg？ithm for detection and isolation of the multiple crosstalk-faults in OSM. Specifically, we develop an algorithm for isolation of the source fault in switc.
ng elements whenever the multiple crosstalk-faults are.
etected in OSM. The proposed algorithm is illustrated by an example of 16$\times$16 OSM.

• Proceedings of the Materials Research Society of Korea Conference
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• 2012.05a
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• pp.58.2-58.2
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• 2012

Heavy hydrocarbon reforming is a core technology for "Dirty energy smart". Heavy hydrocarbons are components of fossil fuels, biomass, coke oven gas and etc. Heavy hydrocarbon reforming converts the fuels into $H_2$-rich syngas. And then $H_2$-rich syngas is used for the production of electricity, synthetic fuels and petrochemicals. Energy can be used efficiently and obtained from various sources by using $H_2$-rich syngas from heavy hydrocarbon reforming. Especially, the key point of "Dirty energy smart" is using "dirty fuel" which is wasted in an inefficient way. New energy conversion laboratory of KAIST has been researched diesel reforming for solid oxide fuel cell (SOFC) as a part of "Dirty energy smart". Diesel is heavy hydrocarbon fuels which has higher carbon number than natural gas, kerosene and gasoline. Diesel reforming has difficulties due to the evaporation of fuels and coke formation. Nevertheless, diesel reforming technology is directly applied to "Dirty fuel" because diesel has the similar chemical properties with "Dirty fuel". On the other hand, SOFC has advantages on high efficiency and wasted heat recovery. Nippon oil Co. of Japan recently commercializes 700We class SOFC system using city gas. Considering the market situation, the development of diesel reformer has a great ripple effect. SOFC system can be applied to auxiliary power unit and distributed power generation. In addition, "Dirty energy smart" can be realized by applying diesel reforming technology to "Dirty fuel". As well as material developments, multidirectional approaches are required to reform heavy hydrocarbon fuels and use $H_2$-rich gas in SOFC. Gd doped ceria (CGO, $Ce_{1-x}Gd_xO_{2-y}$) has been researched for not only electrolyte materials but also catalysts supports. In addition, catalysts infiltrated electrode over porous $La_{0.8}Sr_{0.2}Ga_{0.8}Mg_{0.2}O_3-{\delta}$ and catalyst deposition at three phase boundary are being investigated to improve the performance of SOFC. On the other hand, nozzle for diesel atomization and post-reforming for light-hydrocarbons removal are examples of solving material problems in multidirectional approaches. Likewise, multidirectional approaches are necessary to realize "Dirty energy smart" like reforming "Dirty fuel" for SOFC.

• Journal of Digital Convergence
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• v.13 no.7
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• pp.367-372
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• 2015

Smartphone game market, smart phone appeared on the market and soon developed rapidly, generation change is happening. Generally, Smartphone games are only short-lived super cell's Clash of Clans is gaining popularity for over two years. So Clash of Clans this game with hold most characteristic elements of the defense and plunder on the subject analyzed the fun factor. In order to analyze the defense and plunder elements were prepared Clash of Clans and Kings of Thieves in cases. Two of the cases, depending on the fun theory Preparation, A sense of space, A solid core machanic, A range of challenge, A range of abilities required to solve the encounter and Skill required in using the abilities were evaluated in the fun factor analysis of defense and plunder elements.

• Elastomers and Composites
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• v.44 no.2
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• pp.98-105
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• 2009

The cochlea of the inner ear has two core components, basilar membrane and hair cells. The basilar membrane disperses incoming sound waves by their frequencies. The hair cells are on the basilar membrane, and they are the sensory receptors generating bioelectric signals. In this paper, a biomimetic technology using ZnO piezoelectric nano-pillar was studied as the part of developing process for artificial cochlea and novel artificial mechanosensory system mimicking human auditory senses. In particular, ZnO piezoelectric nano-pillar was fabricated by both low and high temperature growth methods. ZnO piezoelectric nano-pillars were grown on solid (high temperature growth) and flexible (low temperature growth) substrates. The substrates were patterned prior to ZnO nano-pillar growth so that we can selectively grow ZnO nano-pillar on the substrates. A multi-physical simulation was also conducted to understand the behavior of ZnO nano-pillar. The simulation results show electric potential, von Mises stress, and deformation in the ZnO nano-pillar. Both the experimental and computational works help characterize and optimize ZnO nano-pillar.