• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.37 no.6
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• pp.550-555
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• 2011

Chromosomal loss of heterozygosity (LOH) is a common mechanism for the inactivation of tumor suppressor genes in human epithelial cancers. LOH patterns can be generated through allelotyping using polymorphic microsatellite markers; however, owing to the limited number of available microsatellite markers and the requirement for large amounts of DNA, only a modest number of microsatellite markers can be screened. Hybridization to single nucleotide polymorphism (SNP) arrays using Affymetarix GeneChip Mapping 10 K 2.0 Array is an efficient method to detect genome-wide cancer LOH. We determined the presence of LOH in oral SCCs using these arrays. DNA was extracted from tissue samples obtained from 10 patients with tongue SCCs who presented at the Hospital of Tokyo Dental College. We examined the presence of LOH in 3 of the 10 patients using these arrays. At the locus that had LOH, we examined the presence of LOH using microsatellite markers. LOH analysis using Affymetarix GeneChip Mapping 10K Array showed LOH in all patients at the 1q31.1. The LOH regions were detected and demarcated by the copy number 1 with the series of three SNP probes. LOH analysis of 1q31.1 using microsatellite markers (D1S1189, D1S2151, D1S2595) showed LOH in all 10 patients (100). Our data may suggest that a putative tumor suppressor gene is located at the 1q31.1 region. Inactivation of such a gene may play a role in tongue tumorigenesis.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.178-178
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• 1994

Hepatitis B Virus (HBV) is a DNA virus with a 3.2kb partially double-stranded genome. The life cycle of the virus involves a reverse transcription of the greater than genome length 3.5kb mRNA. This pegenomic RNA contains all the genetic information encoded by the virus and functions as an intermediate in viral replication. Tumor suppressor p53 has previously been shown to interact with the X-gene product of the HBV, which led us to hypothesize that p53 may act as a negative regulator of HBV replication and the role of the X-gene product is to overcome the p53-mediated restriction. As a first step to prove the above hypothesis, we tested whether p53 represses the propagation of HBV in in vitro replication system. By transient cotransfection of the plasmid containing a complete copy of the HBV genome and/or the plasmid encoding p53, we found that the replication of HBV is specifically blocked by wild-type p53. The levels of HBV DNA, HBs Ag and HBc/e Ag secreted in cell culture media were dramatically reduced upon coexpresion of wild-type p53 but not by the coexpression of the mutants of p53 (G154V and R273L). Furthermore, levels of RNAs originated from HBV genome were repressed more than 10 fold by the cotransfection of the p53 encoding plasmid. These results clearly states that p53 is a nesative regulator of the HBV replication. Next, to addresss the mechanism by which p53 represses the HBV replication, we performed the transient transfection experiments employing the pregenomic/core promoter-CAT(Chloramphenicol Acetyl Transferase) construct as a reporter. Cotransfection of wild-type p53 but not the mutant p53 expression plasmids repressed the CAT activity more than 8 fold. Integrating the above results, we propose that p53 represses the replication of HBV specifically by the down-regulation of the pregenomic/core promoter, which results in the reduced DNA synthesis of HBV. Currently, the mechanism by which HBV overcomes the observed p53-mediated restriction of replication is tinder investigation.

• The Transactions of the Korea Information Processing Society
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• v.7 no.1
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• pp.74-87
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• 2000

PVM (parallel virtual machine) provides a programming environment that allows a collection of networked workstations to appear as a single parallel computational resource. The performance of parallel applications in this environment depends on the performance of data transfers between tasks. In this paper, we present a new Myrinet-based communication model of PVM that improves PVM communication performance over a high-speed Myrinet LAN. The proposed PVM communication model adopts a communication mechanism that allows any user-level process to directly access the network interface board without going through UDP/IP protocol stacks in the kernel. This mechanism provides faster data transfers between PVM tasks over the Myrinet since it avoids data copy overhead from kernel (user space) to user space (kernel) and reduces communication latency due to network protocol software layers. We implemented EPVM (Enhanced PVM), our updated version of the traditional PVM using UDP/IP, that is based on the proposed communication model over the Myrinet. Performance results show EPVM achieves communication speed-up of one to two over the traditional PVM.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.12 no.1
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• pp.39-45
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• 2001

Background and Objectives : A post-pubescent male classical singer has lower vocal register than a female classical singer. Countertenors who can produce higher vocal register like female classical singers with their falsetto voice and head resonance are recently active. The general purpose of this study is to analyze voice of countertenors and to determine the differences with those of classical singers. Materials and Methods : Four countertenors in Korea were examined using a videostrobos-copy and their voice were analyzed using aerodynamic, acoustic and voice range profile methods. Results and Conclusion : Countertenors could produce elevated fundamental frequency, voice intensity and mean air flow rate using large pulmonary capacity and head voiced falsetto. It means the presence of greater energy in countertenor is due to the more efficient conversion of the air flow to acoustic energy. But, they had unstable amplitude perturbation per each vocal cycle. The results indicated that countertenor is the acoustic products of different laryngeal mechanism with other classical register and it can be recognized as one of the registers of male classical singers.

• Proceedings of the Korean System Dynamics Society
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• 1999.08a
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• pp.105-132
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• 1999

If a system such as a port has a large boundary and complexity, and the system's substance is considered as a black box, forecast accuracy will be very low. Furthermore various components in a port exert significant influence on each other. To copy with these problem the form of structure models were introduced by using SD method. The Competitive Ports Model had several sub-systems consisting of each Unit Port models, and each Unit Port model was made by quantitative, qualitative factors and their feedback loops. The fact that all components of one port have influence on the components of the other ports should be taken into account to construct Competitive Port Models. However, with the current approach that is impossible, and in this paper, therefore, models were simplified by HFP adapted to integrate level variables of unit port models. Although many studies on modelling of port competitive situation have been conducted, both theoretical frame and methodology are still very weak. In this study, a new algorithm called ESD(Extensional System Dynamics) for the evaluation of port competition was presented, and applied to simulate port systems in northeast Asia.

• Journal of the Korean Society of Industry Convergence
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• v.3 no.2
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• pp.167-178
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• 2000

Software RAID(Redundant Arrays of Inexpensive Disks) is defined as a storage system that provides capabilities of hardware RAID, and guarantees high reliability as well as high performance. In this paper, we propose an enhanced disk scheduling algorithm and a scheme to guarantee reliability of data. We also design and implement software RAID by utilizing these mechanism to develop a storage system for multimedia applications. Because the proposed algorithm improves a defect of traditional GSS algorithm that disk I/O requests arc served in a fixed order, it minimizes buffer consumption and reduces the number of deadline miss through service group exchange. Software RAID also alleviates data copy overhead during disk services by sharing kernel memory. Even though the implemented software RAID uses the parity approach to guarantee reliability of data, it adopts different data allocation scheme. Therefore, we reduce disk accesses in logical XOR operations to compute the new parity data on all write operations. In the performance evaluation experiments, we found that if we apply the proposed schemes to implement the Software RAID, it can be used as a storage system for small-sized video-on-demand servers.

• Parasites, Hosts and Diseases
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• v.51 no.5
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• pp.497-502
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• 2013

Autophagy-related protein 8 (Atg8) is an essential component of autophagy formation and encystment of cystforming parasites, and some protozoa, such as, Acanthamoeba, Entamoeba, and Dictyostelium, have been reported to possess a type of Atg8. In this study, an isoform of Atg8 was identified and characterized in Acanthamoeba castellanii (AcAtg8b). AcAtg8b protein was found to encode 132 amino acids and to be longer than AcAtg8 protein, which encoded 117 amino acids. Real-time PCR analysis showed high expression levels of AcAtg8b and AcAtg8 during encystation. Fluorescence microscopy demonstrated that AcAtg8b is involved in the formation of the autophagosomal membrane. Chemically synthesized siRNA against AcAtg8b reduced the encystation efficiency of Acanthamoeba, confirming that AcAtg8b, like AcAtg8, is an essential component of cyst formation in Acanthamoeba. Our findings suggest that Acanthamoeba has doubled the number of Atg8 gene copies to ensure the successful encystation for survival when 1 copy is lost. These 2 types of Atg8 identified in Acanthamoeba provide important information regarding autophagy formation, encystation mechanism, and survival of primitive, cyst-forming protozoan parasites.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.11a
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• pp.147-172
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• 1994

Aziridinylbenzoquinones such as 3, 6-diaziridinyl-1, 4-benzoquinone (DZQ) and its 2, 5-methyl analog (MeDZQ) require bioreductive activation in order to elicit their anticancer activities. To determine the involvement of DTD in the activation of these drugs, we have used a ligation-mediated polymerase chain reaction to map the intracellular alkylation sites in a sing1e copy gene at the nucleotide level. We have performed this analysis in two human colon carcinoma cells, one proficient (HT-29) and one deficient (BE) in DT-diaphorase (DTD) activity. In the DTD proficient HT-29 cell line, DZQ and MeDZQ were found to alkylate both 5'-(A/T)G(C)-3' and 5'-(A/T)A-3' sequences. This is consistent with the nucleotide preferences observed when DZQ and MeDZQ are activated by purified DTD to reactive metabolites capable of alkylating DNA in vitro [Lee, C. -S., Hartley, J. A., Berardini, M. D., Butler, J., Siegel., D., Ross, D., & Gibson, N. W. (1992) Biochemistry, 31: 3019-3025]. Surprisingly in the DTD-deficient BE cell line a pattern of alkylation induced by DZQ and MeDZQ similar to that observed in the DTD-proficient HT-29 cells was observed. This suggests that reductive enzymes other than DTD can be involved in activating DZQ and MeDZQ to DNA reactive species in vivo.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.6 no.6
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• pp.937-942
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• 2002

The use of digital imaging technique and digital contents based on internet has grown rapidly for last several years, and the needs of digital image protection become more important. For the purpose of copyright protection on digital image, the verification of authentication techniques like content authentication, ownership authentication, illegal copy and etc are needed. Digital watermarking, the invisible encryption technique to insert digital watermark into image the sophisticated perceptual information should be used for providing transparency and robustness of images on watermarking process. In this paper, we implement the algorithm for preventing forged attack, ownership protection and authentication by transforming the wavelet algorithms in frequency domain in terms of human visual system.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.31 no.11
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• pp.963-970
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• 2007

The objectives of this study are to examine a bubble trap mechanism of the turbidimeter for low turbidity and to acquire it's technology concerned. Reynolds-Averaged Wavier-Stokes equation and Laglangian discrete phase model were applied to analyze a flow field in the bubble trap. 3D hybrid grid system was used to simulate the flow field of bubble trap and numbers of it's node point are about 110,000. From the comparison between the standard $k-{\varepsilon}$ model and the laminar state, it was found that the former estimated less the velocity in the outlet of bubble trap than the latter did, and that the former estimated more the shear stress at the wall of bubble trap than the latter did. And, it was possible to visualize the path of bubbles in the bubble trap and to copy the removal process of bubbles out bubble trap. Also, it was found that nearly most of bubbles in the bubble trap disappeared.