• Title/Summary/Keyword: Coprinus

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Transformation of Pleurotus sajor-caju by Complementation of PABA Requiring Mutant (여름 느타리버섯에서 PABA 변이주의 Complementation에 의한 형질전환)

  • Byun, Myung-Ok;Chung, Jong-Chun;You, Chang-Hyun;Cha, Dong-Yeul;Lee, Du-Hyung
    • The Korean Journal of Mycology
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    • v.25 no.3 s.82
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    • pp.233-237
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    • 1997
  • A PABA auxotroph of Pleurotus sajor-caju were transformed to prototrophy by using a plasmid containing pab 1 gene from Coprinus. The efficiencies of transformation of Pleurotus sajor-caju was five transformants per ${\mu}g$ of plasmid DNA. Southern blot analysis of DNA extracted from transformants demonstrated that plasmid DNA was integrated into the chromosomal DNA in multiple tandem copies. Progenies of heterokaryons between transformants of PABA and other auxotropic strains produced pab-progeny, which indicated that integration occurred at a site(s) other than the resident pab biosynthetic gene.

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The Studies on the Heterogenous Gene Expression in Schizophyllum commune (치마버섯에서 이형 유전자 발현에 관한 연구)

  • Park, Dong-Chul;Kim, Hyun-Jeong;Kim, Ok-Mi;Bae, Jun-Tae;Park, Sun-Hee;Lee, Byeung-Hun;Lee, Kap-Rang
    • The Korean Journal of Mycology
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    • v.26 no.1 s.84
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    • pp.103-107
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    • 1998
  • The trp2 gene encoding trifunctional enzyme in Coprinus cinereus was investigated the expression in the heterothallic mushroom species. To identify the homology of trp2 gene to Schizophyllum commune and Pleurotus ostreatus, southern hybridization was performed with plasmid pHIONA8 containing C. cinereus trp2 gene as a probe, which resulted in a strong signal indicating an homologous sequence to the chromosomal DNA of S. commune. About 50 transformants per dish was appeared in the complementation test by pHIONA8 using S. commune tryptophan auxotroph as host. In the mating test between transformants and other mating type alleles, the fruiting body of S. commune was formed at $30^{\circ}C$ in $2{\sim}3$ weeks.

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Preparation and Characteristics of Alkaline -active Cellulases from Coprinaceae

  • Lee, Jung-Kyung;Eom, Tae-Jin
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.31 no.3
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    • pp.68-76
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    • 1999
  • Coprinus cinereus 2249 producing alkaline-active cellulase was screened from 29 species of Corpinaceae and constitutively produced alkaline carboxymethyl cellulase (CMCase) and filter paper cellulase (Fpase). When cultivated at pH 9.0, 25$^{\circ}C$ and 5 days, copnnus cinereus 2249 produced higher alkaline activity on 0.5% CMC, 2% wheat bran as carbon source and 0.5% peptone, 0.05% yeast extract as nitrongen source compared with other culture conditions. The level of cellulase production was higher in the presence of wheat bran than in the presence of CMC. The optimum temperature and pH for alkaline -active cellulase activity weas 50$^{\circ}C$ and 9, 0, respectively.

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Recycling of Waste Paper with Alkaline Cellulolytic Enzymes(I) - Preparation and characteristics of cullulolytic enzymes- (호알카리성 목질분해 효소를 이용한 폐지재생(제1보) -목질분해 효소의 단리 및 특성-)

  • 강석현;이중명;박성배;엄태진
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.35 no.3
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    • pp.66-73
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    • 2003
  • Alkaline cellulolytic enzymes are prepared from Coprinus cinereus 2249. Recovery method of enzyme protein from cultured medium and effect factors on enzyme activity of protein were investigated. The results could summarized as follows, \circled1 Amount of enzyme protein from cultured medium was highest in incubation with shaking and addition of skim milk. \circled2 Protein from cultured medium was alkaline enzymatic protein which shows the highest activity at pH 9.0. \circled3 The most effective recovery method of enzyme protein was the precipitation of protein by addition of cultured medium of protein in ethanol. \circled4 The enzyme activity was enhanced by tween-80 and decreased with $Al_2(SO_4)_3$, $H_2O_2$et al, and was little changed with metal ions except $Hg^{++}$.

Recycling of Waste Paper with Alkaline Cellulolytic Enzyme(III) - Elimination of sticky contaminants and bleaching of recycling pulp- (호알칼리성 목질 분해 효소를 이용한 페지 재생(제3보) -페지의 이물질 제거 및 표백-)

  • 강석현;이중명;박성배;엄태진
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.36 no.1
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    • pp.30-36
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    • 2004
  • Waste papers(MOW and ONP) were deinked with alkaline cellulolytic enzyme from Coprinus cinereus 2249. The effectiveness of alkaline enzyme on sticky contaminant removal and bleaching of recycling pulp was investigated. The conclusions obtained from the results are as follows. The brightness of deinked pulps MOW and ONP was most high in 0.4IU enzyme treatment. \circled2 The removal effect of sticky contaminant was enhanced with alkaline cellulolytic enzyme treatment. Bleaching with sodium hydrosulfite was most effective in one and two stage bleaching after enzyme treatment of mixed wastepaper of MOW and colored paper. \circled4 The brightness of alkaline enzyme deinked ONP was increased 20% with $H_2O$$_2$ bleaching.

RECYCLING OF WASTEPAPER WITH ALKALINE ENZYME FROM COPRINACEAE SP.

  • Eom, Tae-Jin;Lee, Jung-Myoung
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 1999.04b
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    • pp.291-295
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    • 1999
  • Coprinus cinereus 2249 that is a kind of basidiomycetes constitutively produced alkaline carboxymethyl cellulase (CMCase), filter paper cellulase (FPase) and xylanase. Crude enzymes prepared with optimal conditions showed higher FPase activity than CMCase activity. The FPase was most active at pH 9 at 50$^{\circ}C$. When applied on deinking of the old newsprint (ONP), it increases the freeness and brightness due to effect of hydrolysis at 0.1% enzyme concentration. Also, The physical properties of deinked pulp were improved.

Notes on Korean Higher Fungi (II) (한국고등균류기(韓國高等菌類記) (II))

  • Lee, Ji-Yul;Cho, Duck-Hyun
    • The Korean Journal of Mycology
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    • v.5 no.2
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    • pp.17-20
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    • 1977
  • Several fungi were collected at Mt. Sobaek, Andong and Euisung the period from 1974 to 1976. These fungi were identified and results are as followingj Lipophyllum fallax (Peck.) $K{\ddot{u}}hn$ et Romagn. Armillariella tabescens (Scop. ex Fr.) Sing., Crepidotus subphaerosporus (Lange) $K{\ddot{u}}hn$. et Romagn., Stereum hiugense Imaz., Coprinus radians (Desm.) Fr., Hygrophorus suzukaensis Hongo and Xylaria carpophila (Pers.) Fr. were listed newly by authors in Korea.

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Characterization of UV-damaged repair genes in cells

  • Choi, In-Soon
    • Journal of Life Science
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    • v.10 no.2
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    • pp.50-54
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    • 2000
  • The RAD4 gene of Saccharomyces cerevisiae is essential for the incision step of UV-induced excision repair. A yeast RAD4 gene has been previously isolated by functional complementation. In order to identify the RAD4 homologous gene from fungus Coprinus cinereus, we have constructed cosmid libraries from electrophoretically separated chromosomes of the C. cinereus. The 13 C. cinereus chromosomes were resolved by pulse-field gel electrophoresis, hybridized with S. cerevisiae RAD4 DNA, and then isolated homologous C. cinereus chromosome. Here, we report the cloning and characterization of fungus C. cinereus homolog of yeast RAD4 gene. Southern blot analysis confirmed that C. cinereus contains the sequence homologous DNA to RAD4 gene and this gene exists as a single copy in C. cinereus genome. When total RNA isolated from C. cinereus cells was hybridized with the 3.4 kb BglII DNA fragment of the S. cerevisiae RAD4 gene, a 2.5 kb of transcript was detected. The isolated gene encodes a protein of 810 amino acids.

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Phylogenetic Relationships of Coprinoid Taxa and an Agaric-like Gastroid Taxon Based on the Sequences of Internal Transcribed Spacer (ITS) Regions (ITS 영역의 염기서열을 이용한 먹물버섯류 및 주름버섯 유사 복균류와의 계통학적 유연관계)

  • Park, Dong-Suk;Go, Seung-Joo;Ryu, Jin-Chang
    • The Korean Journal of Mycology
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    • v.27 no.6 s.93
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    • pp.406-411
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    • 1999
  • The phylogenetic relationships of black-spored basidioid taxa (mainly coprinoid taxa) and an agaric-like gastroid taxon were studied. The sequences of internal transcribed spacer regions (ITS) partially including 17S, 25S and 5.8S from 14 species (Coprinus comatus, C. atramentarius, C. micaceus, C. cinereus, C. disseminatus, C. rhizophorus, C. radians, C. echinosporus, Psathyrella candolleana, Podaxis pistillaris, Conocybe lactea, Bolbitius demangei, Agaricus balzei, and Stropharia rugosoannulata) were compared. The reciprocal homologies of ITS sequences among these species were in the range of $38.7{\sim}77.2%$. Black-spored taxa were classified into four clusters. Cluster I comprised C. micaceus, C. radians, and C. disseminata. Cluster II is consisted of C. cinereus, C. echinosporus, C. rhizophorus, and C. atramentarius. On the other hand, C. comatus is in cluster III with Agaricus balzei and Podaxis pistillaris even though this species is belonging to the section Coprinus in morphological aspect. Psathyrelloid taxon is included in cluster II. The question of the origin of secotioid (agaric-like) fungi has been taken, though largely on theoritical suggestions as to whether gastroid taxa give rise to agarics with secotioid taxa as intermediates or whether secotioid taxa are evolutionary novelities arising from many distinct groups of agarics. In this relationship, it was shown that secotioid taxon evolved from within agaric species.

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