• The Microorganisms and Industry
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• v.14 no.3
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• pp.12-14
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• 1988

빛에 의한 생물체의 분화는 작용파장별로 분류 할 때 고등식물에서 흔히 나타나는 붉은 빛에 의한 분화와 곰팡이류에서 볼 수 있는 푸른빛에 의한 것 두 종류가 있다. 곰팡이는 Chlorophyll이나 Phytochrome이 없어 붉은ㅅ개 파장을 흡수하는 phytochrome에 의한 분화는 없으나 푸른색은 곰팡이의 생활사에 중요한 요인이 된다. 예를 들면 Phycomyces와 Trichoderma의 photophorogenesis, Cyathus와 Coprinus의 fruiting body형성, Poycomyces와 Neurospora의 photocarotenogenesis, Phycomyces와 Pilobolus의 phototropism등 곰팡이의 metabolism과 development가 푸른 빛에 의하여 영향을 받는다. 한편 곰팡이의 포자및 포자생성기관은 녹색, 갈색, 흑색 등 phenoloxidase의 산물인 melanin 색소를 가지는 경우가 많으며 그 예로는 Podospora, Schizophyllum, Aspergillus 등과 같이 reproduction 할 때 phenoloxidase가 직접적 혹은 간접적으로 연관되어 있다. 버섯형성균류의 하나인 Coprinus congregatus는 버섯형성과정에서 빛을 필요로 하며 이때 phenoloxidase가 어떠한 역할을 하는지 실험하였다.

• Archives of Pharmacal Research
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• v.2 no.1
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• pp.25-33
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• 1979

In order to find out nutritional effects of Korean edible mushrooms on the multiplication of tissue cells, the alcohol extracts and acid bydrolysates of eleven species of mushrooms were added to Earle's ESS. A comparison of the respective multiplications of HeLa cells in this solution and in control solution of TC=199. Yielded the following result: The alcohol extracts and acid hydrolysates of a Coprinus comatur, Agaricus campestris, Agaricus bisporus, Lentinus edodes, Tricholoma matsutake, Pleurotus ostretus, Ramaria botrytis and Pholiota nameko influenced favorably the maintenance of the normal form and monolayer of HeLa cells. The growth curves of HeLa cells in the cultures containing, respectively, the alcohol extracts and acid hydrolysates of these eight mushrooms showed that five species, i.e., Coprinus comatus, Agaricus compestris, Agaricus bisporus, Lentinus edodes and Tricholoma matsutake effected an excellent multiplication and that the other three species were less effective than those five species. As to the effects on the cell multiplication, no marked difference was observed between the alcohol extracts and the acid hydrolysates of the mushrooms tested.

• Korean Journal of Plant Resources
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• v.23 no.1
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• pp.115-121
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• 2010

Many fungi were collected in Mt. Paekdu areas from May, 2002 to September, 2008 and were identified. As the resulting, the following species were unrecorded to Korea ; Clitocybe alnetorum, C. catinus, C. metachroa, C. odora var. alba, C. phyllophilla, Amanita muscaria var. formosa, A. pantherina var. lutea, Coprinus semitalis, Agrocybe dura, Inocybe grammata, Cortinarius epipolius and C. nemorensis. They were added list of Korean fungal resources.

• Proceedings of the Zoological Society Korea Conference
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• 1997.10b
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• pp.273.1-273
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• 1997

No Abstract, See Full Text

• Journal of Microbiology and Biotechnology
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• v.27 no.4
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• pp.775-784
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• 2017

A neutral xylanase (CcXyn) was identified from Coprinus cinereus. It has a single GH10 catalytic domain with a basic amino acid-rich extension (PVRRK) at the C-terminus. In this study, the wild-type (CcXyn) and C-terminus-truncated xylanase ($CcXyn-{\Delta}5C$) were heterologously expressed in Pichia pastoris and their characteristics were comparatively analyzed with aims to examine the effect of this extension on the enzyme function. The circular dichorism analysis indicated that both enzymes in general had a similar structure, but $CcXyn-{\Delta}5C$ contained less ${\alpha}-helices$ (42.9%) and more random coil contents (35.5%) than CcXyn (47.0% and 32.8%, respectively). Both enzymes had the same pH (7.0) and temperature ($45^{\circ}C$) optima, and similar substrate specificity on different xylans. They all hydrolyzed beechwood xylan primarily to xylobiose and xylotriose. The amounts of xylobiose and xylotriose accounted for 91.5% and 92.2% (w/w) of total xylooligosaccharides (XOS) generated from beechwood by CcXyn and $CcXyn-{\Delta}5C$, respectively. However, truncation of the C-terminal 5-amino-acids extension significantly improved the thermostability, SDS resistance, and pH stability at pH 6.0-9.0. Furthermore, $CcXyn-{\Delta}5C$ exhibited a much lower $K_m$ value than CcXyn (0.27 mg/ml vs 0.83 mg/ml), and therefore, the catalytic efficiency of $CcXyn-{\Delta}5C$ was 2.4-times higher than that of CcXyn. These properties make $CcXyn-{\Delta}5C$ a good model for the structure-function study of $({\alpha}/{\beta})_8$-barrel-folded enzymes and a promising candidate for various applications, especially in the detergent industry and XOS production.

• The Korean Journal of Mycology
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• v.22 no.4
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• pp.421-426
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• 1994

Some Agaric fungi were collected from the area of Suwon, Mt. Taewha and Mt. Yongmoon in Kyonggi-do and Chongpyong temple in Kangwon-do. Among them, one genus and five taxa of Agaricales were confirmed new to Korea. In this paper we registered five texa of Agricales in detailed description such as Hygrocybe firma (Berk. & Br.) Sing. var. firma Sing.; Hohenbuehelia petalodes (Bull.: Fr.) Schulzer; Cystoagaricus strobilomyces (Mull.) Sing.; Coprinus domesticus (Bolt.: Fr.) S.F.Gray and Inocybe acutata T. Koba.& E.Naga.. The one genus new to Korea is Cystoagaricus Sing.

• The Korean Journal of Mycology
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• v.21 no.1
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• pp.64-72
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• 1993

Fresh fungi were collected during five field trips to Ullung Island from October of 1989 to September of 1991. Through the observation of some agaric fungi, thirty mushrooms were identified to the species and are listed as below. Among them, three species, Crepidotus geophilus, Mycena elegans, ana Mycena carolinensis were confirmed new to Korea and are registered here with descriptions.

• The Korean Journal of Mycology
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• v.41 no.4
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• pp.212-217
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• 2013

Unusual mushrooms were observed in the greenhouses of vegetables Chinese chive, cucumber, melon and Korean dandelion in four locations of Korea in 2012. The mushrooms usually grew with the vegetables cultivated on beds in the greenhouses. Occurrence aspects of the mushrooms in the greenhouses and effect of the mushrooms on growth and quality of the vegetables were investigated. Mushroom samples were collected from the beds in the greenhouses and identified based on their morphological characteristics. The mushroom samples collected from the greenhouse of Chinese chive were identified as Coprinus aokii, those from the greenhouse of cucumber as Cristinia eichleri and Coprinus sp., those from the greenhouse of melon as Leucocoprinus cretaceous, Panaeolus fimicola and Conocybe sp., and those from the greenhouse of Korean dandelion as Conocybe rickenii f. tetrasporique. Out of the seven mushrooms identified, L. cretaceus, C. rickenii and C. eichleri were first found in Korea. The investigation results revealed that the mushrooms were originated from compost or soil used in the beds, and showed that C. aokii caused sooty mold on leaves of Chinese chive, and C. rickenii f. tetrasporique hindered growth of Korean dandelion and caused sooty mold on leaves of the plant. The other mushrooms had no effect on growth and quality of the vegetables.

• Journal of Life Science
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• v.14 no.6 s.67
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• pp.1023-1027
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• 2004

The RAD3 gene of Saccharomyces cerevisiae is essential for the incision step of UV-induced excision repair. An yeast RAD3 gene has been previously isolated by functional complementation. In order to identify the RAD3 homologous gene from fungus Coprinus cinereus, we have constructed cosmid libraries from electrophoretically separated chromosomes of the C. cinereus. The 13 C. cinereus chromosomes were resolved by pulse-field gel electrophoresis, hybridized with S. cerevisiae RAD3 DNA, and then isolated RAD3 homologous DNA from C. cinereus chromosome. The RAD3 homolog DNA was contained in 3.2 kb DNA fragment. Here, we report the results of characterization of a fungus C. cinereus homolog to the yeast RAD3 gene. Southern blot analysis confirmed that the C. cinereus chromosome contains the RAD3 homolog gene and this gene exists as a single copy in C. cinereus genome. When total RNA isolated from the C. cinereus cells were hybridized with the 3.4 kb PvuII DNA fragment of the S. cerevisiae RAD3 gene, transcripts size of 2.8 kb were detected. In order to investigate whether the increase of the amount of transcripts by DNA damaging agent, transcript levels were examined after treating agents to the cells. The level of transcripts were not increased by untraviolet light (UV). This result indicated that the RAD3 homologous gene is not UV inducible gene. Gene deletion experiments indicate that the HRD3 gene is essential for viability of the cells and DNA repair function. These observations suggest an evolutionary conservation of other protein components with which HRD3 interacts in mediating its DNA repair and viability functions.

• Korean Journal of Veterinary Service
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• v.37 no.1
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• pp.51-58
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• 2014

The aim of the study was to investigate the anti-obesity effect of Coprinus comatus (CC) in high-fat diet-fed Zucker rat (fa/fa). Obesity was induced by feeding on high-fat diet (HFD) containing 60% kcal fat for 10 weeks, in which CC extracts were administrated through the gastrointestinal tract at a concentration of 200 mg/kg BW/day for 10 weeks. The total polyphenol and flavonoid contents of CC extracts were found to be $18.5{\pm}1.1mg$ of catechin equivalent/g, and $5.24{\pm}0.54mg$ of quercetin equivalent/g extract, respectively. The DPPH, ABTS, and hydroxyl radical scavenging activities of CC extracts were 15.34 %, 17.25%, and 16.18%, respectively. In animal study, CC administration significantly reduced the body weight, while there were no significant differences in the daily food intake between the HFD-fed Zucker rats and HFD plus CC-fed rats. CC treatment decreased epididymal and perirenal fat weights in HFD-fed Zucker rats. Significant decreases in the levels of triglyceride and total cholesterol in the serum and liver were observed in the CC-treated group compared with HFD-fed Zucker rats. Serum HDL-cholesterol levels in the CC-treated group were increased compared with the HFD-fed groups. Serum AST and ALT activities in the CC group were significantly lower than those of the HFD-fed group. Taken together, these data demonstrated that CC has potential in preventing high-fat diet induced obesity and is a good candidate for an anti-obesity agent.