• Journal of the Korean Wood Science and Technology
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• 제30권2호
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• pp.151-157
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• 2002

갈색부후균 Tyromyces palustris를 이용하여 CCA, CCFZ처리 목재에서 구리를 제거하였다. 균에 의한 구리의 제거효율을 높이기 위한 전처리 방법으로서 증기압처리와 수산처리를 검토하였으며, 적정 배양 방법을 찾기 위하여 진탕배양, 고체배양, 정치배양에서 구리 제거율을 비교하였다. 전처리 방법에서, 증기압 처리만으로는 효과적인 구리제거가 불가능하였으며, 수산처리는 구리제거율은 낮았으나 수산처리 후 균처리를 하면 제거율이 향상되었다. 배양 방법에서는 정치 배양의 구리 제거효율이 높게 나타났다. 대량 배양을 위한 공기부양식 생물반응기에 의한 구리의 제거율은 7일 배양 이후 61%의 제거율을 보였다. T. palustris에 의한 구리제거기작을 탐색하기 위하여 배양액 중에 생성된 물질을 LC/Mass 분석 결과 T. palustris에서 분비되어진 수산과 방부처리 목재 중의 구리가 결합된 수산구리 착염체의 형성이 배양액 중에 존재함이 확인됨으로 균체 외 수산의 작용이 처리재의 구리제거에 크게 관여함이 밝혀졌다.

• 대한화학회지
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• 제36권5호
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• pp.709-719
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• 1992

이핵성 네자리 Schiff base 의 cobalt(II), nickel(II) 및 copper(II) 착물인 [Co(II)_2(TSBP)(L)_4], [Ni(II)_2(TSBP)(L)_4] 및 [Cu(II)_2(TSBP)] (TSBP: 3,3',4,4'-tetra(salicylideneimino)-1,1'-biphenyl, L: Py, DMSO 및 DMF)들을 합성하여 원소분석, IR-spectrum, UV-visible spectrum, T.G.A. 및 D.S.C.를 측정하여 이핵성 착물로 주어짐을 확인하였다. 지지전해질로서 0.1M TEAP-L (L; Py, DMSO 및 DMF)에서 1mM-착물용액의 순환전압-전류법과 D.P.P.법으로 전기화학적 성질을 측정한 결과 이핵성 cobalt(II) 착물은 일전자의 확산 지배적인 네단계의 산화-환원과정이 Co(III)_2 {^\longrightarrow \\_\longleftarrow^e^-}Co(III)Co(II)_2{^\longrightarrow \\_\longleftarrow^e^-}Co(II){^\longrightarrow \\_\longleftarrow^e^-}Co(I){^\longrightarrow \\_\longleftarrow^e^-}Co(I)_2 으로 일어나고 이핵성 nickel(II) 및 copper(II) 착물들은 일전자의 확산지배적인 두 단계의 산화-환원과정이 M(II)_2 {^\longrightarrow \\_\longleftarrow^e^-}M(I)M(I){^\longrightarrow \\_\longleftarrow^e^-}M(I)_2 (M; Ni 및 Cu)으로 일어남을 알았다.

• Journal of Microbiology and Biotechnology
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• 제25권4호
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• pp.503-510
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• 2015

The iLOV protein belongs to a family of blue-light photoreceptor proteins containing a light-oxygen-voltage sensing domain with a noncovalently bound flavin mononucleotide (FMN) as its chromophore. Owing to advantages such as its small size, oxygen-independent nature, and pH stability, iLOV is an ideal candidate over other reporter fluorescent proteins such as GFP and DsRed. Here, for the first time, we describe the feasibility of applying LOV domain-based fluorescent iLOV as a metal sensor by measuring the fluorescence quenching of a protein with respect to the concentration of metal ions. In the present study, we demonstrated the inherent copper sensing property of the iLOV protein and identified the possible amino acids responsible for metal binding. The fluorescence quenching upon exposure to Cu²⁺ was highly sensitive and exhibited reversibility upon the addition of the metal chelator EDTA. The copper binding constant was found to be 4.72 ± 0.84 µM. In addition, Cu²⁺-bound iLOV showed high fluorescence quenching at near physiological pH. Further computational analysis yielded a better insight into understanding the possible amino acids responsible for Cu²⁺ binding with the iLOV protein.

• Asian-Australasian Journal of Animal Sciences
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• 제23권5호
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• pp.573-579
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• 2010

This study was conducted to determine the effects of different copper (inorganic and organic) and energy (tallow and glycerol) sources on growth performance, nutrient digestibility, gas emission, diarrhea incidence, and fecal copper concentration in growing pigs by using a 2${\times}$2 factorial design. In this trial, 96 pigs (63 d of age) were employed, with an average initial weight of 28.36${\pm}$1.14 kg. The dietary treatments were i) basal diet with 134 ppm copper (Korea recommendation) as $CuSO_4$+tallow; ii) basal diet with 134 ppm Cu as $CuSO_4$+glycerol; iii) basal diet with 134 ppm copper as CuMet+tallow; and iv) basal diet with 134 ppm copper as CuMet+ glycerol. Throughout the entire experimental period, no differences were noted among treatment groups with regard to the magnitude of improvement in ADG (average daily gain), ADFI (average daily feed intake) and G/F (gain:feed) ratios. The nitrogen (N) digestibility of pigs fed on diets containing organic copper was improved as compared with that observed in pigs fed on diets containing inorganic copper (p<0.05). An interaction of copper${\times}$energy was observed in the context of both nitrogen (p<0.05) and energy (p<0.01) digestibility. Ammonia emissions were significantly lower in the organic copper-added treatment groups than in the inorganic copperadded treatment groups (p<0.05). Mercaptan and hydrogen sulfide emissions were reduced via the addition of glycerol (p<0.05). No significant effects of copper or energy source, or their interaction, were observed in reference to diarrhea appearance and incidence throughout the entirety of the experimental period. The copper concentration in the feces was significantly lower in the organic copper source treatment group than was observed in the inorganic copper source treatment group (p<0.05). The results of this experiment show that organic copper substituted for inorganic copper in the diet results in a decreased fecal copper excretion, but exerts no effect on performance. The different energy (tallow and glycerol) sources interact with different copper sources and thus influence nutrient digestibility. Glycerol supplementation may reduce the concentrations of odorous sulfuric compounds with different Cu sources.

• 한국염색가공학회지
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• 제7권1호
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• pp.72-79
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• 1995

The effect of copper sulfate/thiourea on the diffusion and the dye exhaustion of two dyes, C. I. Acid Orange 7 and C. I. Food Yellow 3 in nylon 6 fiber has been studied. The results obtained from the experimetal are as follows : 1) Copper sulfate reacted with thiourea at constant temperature and then generated the colorless trasparent coordination compound, and then combinated end group of carboxyl of nylon 6 fiber. 2) The coordination compound [$Cu_{2}$($TU_{6}$)] ($(SO_{4})_2$) is conducted at pH 5-6 in solution intensity and repulsed the sulfonic acid group of dyes, and therefore the diffusion of dyes is restraint. 3) Dye uptake and diffusion coefficient were decreased in the order of untreated > copper sulfate/thiourea-treated > tannin-treated.

• Bulletin of the Korean Chemical Society
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• 제27권7호
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• pp.1079-1082
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• 2006

• 자원환경지질
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• 제18권4호
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• pp.301-308
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• 1985

I.P exploration is conducted mainly at the alluvium covered granodiorite stock of the Red Hill area at the Dongjeom coal mine, employing Canadian Mcphar equipments of variable frequencies-domain method along a total of 8.5 survey line. Mineralization zone is found by LP anomalies along the I.P the profile of frequency mode. Comparing with the past drilling data, the cause of each anomly is furthermore identified as copper bearing mineralized zones. As alteration and mineralization cover all over the Red-Hill altered grandiorite, copper bearing sulfide veinlets and strings filled out the fractured and altered zones.

• 한국표면공학회지
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• 제29권6호
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• pp.803-808
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• 1996

Material and electrical characteristies of copper thin films prepared by metal organic chemical vapor deposition (MOCVD) have been investigated for interconnection applications in ultra large scale integration circuits (ULSI). The copper films have been deposited a TiN substrates using a metal organic precursor, hexafluoro acetylacetonate trimethyvinylsilane copper, VTMS(hfac)Cu (I). Deposition rate, grain size, surface morphology, and electrical resistvity of the copper films have been measuredfrom samples prepared at various experimental conditions, which include substrate temperature, chamber pressure, and carrier gas flow rate. Results of the experiment showed that the electrical property of the copper films is closely related to the crystallinity of the films. Lowest electrical resistivity, $2.4{\mu}{\Omega}.cm$ was obtained at the substrate temperature of $180^{\circ}C$, but the resistivity slightly increased with increasing substrate temperature due to the carbon content along the copper grain boundaries.

• Nuclear Engineering and Technology
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• 제50권3호
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• pp.462-469
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• 2018

The background spectrum of a $3^{{\prime}{\prime}}{\times}3^{{\prime}{\prime}}$ NaI(Tl) well-type scintillation SILENA detector was measured without shielding, in 6 cm thick lead shielding, and with 2 mm thick electrolytic copper covering the detector inside the lead shielding. The relative remaining background of the lead shield lined with copper was found to be ideal for low-level environmental radioactive spectroscopy. The background total count rate in the (20-2160 KeV) was reduced 28.7 times by the lead and 29 times by the Cu + Pb shielding. The effective reduction of background (1.04) by the copper mainly appeared in the energy range from X-ray up to 500 KeV, while for the total energy range the ratio is 1.01 relative to the lead only. In addition, a strong relation between the full-energy peak absolute efficiency and the detector well height was found using gamma-ray isotropic radiation point sources placed inside the detector well. The full-energy peak efficiency at a midpoint of the well (at 2.5 cm) is three times greater than that on the detector surface. The energy calibrations and the resolution of any single energy line are independent of the locations of the gamma source inside or outside of the well.

• 미생물학회지
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• 제47권2호
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• pp.158-162
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• 2011

Proteus mirabilis 전사 조절($\underline{P}$roteus $\underline{m}$irabilis $\underline{t}$ranscription $\underline{r}$egulator ) 단백질의 중금속 결합 부위에 대한 아미노산 서열분석에서 PMTR 단백질은 ZntR (아연 저항성) 단백질이 아닌 CueR (구리 저항성) 단백질과 동일한 환경이다. 그리고 겔시프트 법(gel shift assay) 실험에 의하면 PMTR 단백질은 Escherichia coli의 zntA (zinc-translocating P-type ATPase gene) 프로모터에 결합하지 않고 copA (copper-translocating P-type ATPase gene) 프로모터와 Proteus mirabilis에서 atpase (copper-translocating P-type ATPase gene) 프로모터에 결합하였다. DNase I protection 실험에서 PMTR 단백질 결합부위와 DNase I 민감성 염기들이 관찰되었다. P. mirabilis atpase 프로모터에서 민감성 염기로 주형가닥(template strand)에서 C와 A 그리고 비주형가닥(non-template strand)에서 G와 C 염기들이다. 이런 민감성 염기들은 다른 MerR 패밀리 단백질에서 또한 관찰되었으며, 이것은 단백질에 의한 DNA bending을 의미한다.