• 제목/요약/키워드: Cooling Therapy

검색결과 45건 처리시간 0.021초

A New Protocol for Effective Cryopreservation of Human Embryonic Stem Cells by a Minimum Volume Cooling Method

  • Kim, Eun-Young;Lee, Keum-Sil;Shin, Hyun-Ah;Park, Sae-Young;Yoon, Ji-Yeon;Kil, Kwang-Soo;Lee, Young-Jae;Kim, Nam-Hyung;Chung, Kil-Saeng
    • 한국발생생물학회:학술대회논문집
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    • 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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    • pp.98-98
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    • 2003
  • Recently, human embryonic stem (hES) cells have become very important resources for ES cell basic research, cell replacement therapy, and other medical applications; thus, efficient cryopreservation methods for these cells are needed. This study examined whether a newly developed minimum volume cooling (MVC) vitrification method, which was tested through cryopreservation of sensitive bovine oocytes, can be used for freezing hES cells. Feeder-free cultured hES cell (MB03) colonies were mechanically dissected into several small clumps following enzymatic treatment. We compared the freezing efficiency of a slow-cooling method using a cryo-module (0.4-0.6C/min, 20-30 clumps/vial) and MVC vitrification using a modified 0.5-ml French mini-straw designated as a MVC straw (>$20,000{\circ}C$/min, 10 clumps/straw) After thawing, in vitro survival of hES cell clumps was higher for MVC-vitrified cells (80.8%, 97/120) than for slow-cooled cells (38.2%, 39/102). Further, the proliferation rate of surviving MVC-vitrified cells was similar to that of control hES cells from 2 weeks after thawing. In addition, vitrified-thawed hES cells demonstrated a normal karyotype, were positively immunostained for surface marker antibodies (AP, SSEA-4 and TRA-1-60) and the Oct-4 antibody, and could differentiate into all three embryonic germ layer cells in vitro. This result demonstrates that hES cell clumps can be successfully cryopreserved by a newly developed MVC vitrification method without loss of human cell characteristics.

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鵝掌風에 關한 文獻的 考察 (A literatual studies on the ajangpung(鵝掌風).)

  • 장기영;노석선
    • 한방안이비인후피부과학회지
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    • 제11권1호
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    • pp.141-161
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    • 1998
  • In the literatual studies on the ajangpung(鵝掌風), the results were as follows. Ajangpung(鵝掌風) is called 'Jangsimpung(掌心風)', is concerned with 'Tinea Manus(手癬)' in western medicine. The pathogenic factors of Ajangpung were stomach-heat & blood dryness, wind-cold-wetness evil, or contact infection etc. The treatments of Ajangpung were used external treatments for dispelling wind-evil & cooling blood, dispelling wetness-evil & parasiticidal therapy. In the frequency of prescription, the most numerous prescription was Yibansan(二礬散) and the next were Yungigo(潤肌膏), Gupungjihwanghwan(祛風地黃丸) etc.

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등속성 운동 후 양측 대퇴부의 피부 온도 변화 (Bilateral Skin Temperature Change of the Anterior Thigh Following Unilateral Isokinetic Exercise)

  • 김선미;오영수;이지은;권혁철
    • 한국전문물리치료학회지
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    • 제2권1호
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    • pp.14-20
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    • 1995
  • The purpose of this study was to measure and compare the skin temperature over the exercised muscle and corresponding non-exercised muscle after unilateral isokinetic exercise using digital thermography. Thirty-two young healthy volunteers with no history of knee injury were tested. After isokinetic exercise at 60 degree per second angular velocity using the right leg in a climatic chamber at ambient temperature of $23-26^{\circ}C$, skin temperature of the anterior thigh was tested. After exercise, the skin temperature of both the right and left leg had fallen significantly. The skin temperature of the exercised leg fell less than that of the non-exercised leg. The fall in skin temperature after work was not due to increased evaporative cooling, but was the result of segmental vasoconstriction probably caused reflexly in the spinal cord by non-thermal afferents from exercising muscle or moving tissues. The effect of thermoregulatory vasodilation was reduced by reflex vasoconstriction caused by non-thermal factors such as catecholamine.

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CURRENT RESEARCH ON ACCELERATOR-BASED BORON NEUTRON CAPTURE THERAPY IN KOREA

  • Kim, Jong-Kyung;Kim, Kyung-O
    • Nuclear Engineering and Technology
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    • 제41권4호
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    • pp.531-544
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    • 2009
  • This paper is intended to provide key issues and current research outcomes on accelerator-based Boron Neutron Capture Therapy (BNCT). Accelerator-based neutron sources are efficient to provide epithermal neutron beams for BNCT; hence, much research, worldwide, has focused on the development of components crucial for its realization: neutron-producing targets and cooling equipment, beam-shaping assemblies, and treatment planning systems. Proton beams of 2.5 MeV incident on lithium target results in high yield of neutrons at relatively low energies. Cooling equipment based on submerged jet impingement and micro-channels provide for viable heat removal options. Insofar as beam-shaping assemblies are concerned, moderators containing fluorine or magnesium have the best performance in terms of neutron accumulation in the epithermal energy range during the slowing-down from the high energies. NCT_Plan and SERA systems, which are popular dose distribution analysis tools for BNCT, contain all the required features (i.e., image reconstruction, dose calculations, etc.). However, detailed studies of these systems remain to be done for accurate dose evaluation. Advanced research centered on accelerator-based BNCT is active in Korea as evidenced by the latest research at Hanyang University. There, a new target system and a beam-shaping assembly have been constructed. The performance of these components has been evaluated through comparisons of experimental measurements with simulations. In addition, a new patient-specific treatment planning system, BTPS, has been developed to calculate the deposited dose and radiation flux in human tissue. It is based on MCNPX, and it facilitates BNCT efficient planning based via a user-friendly Graphical User Interface (GUI).

乾癬에 對한 東.西醫 文獻的 硏究 (The Oriental and Occidental bibliographic Study of Psoriasis)

  • 김남권;황충연;임규상
    • 한방안이비인후피부과학회지
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    • 제12권1호
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    • pp.154-178
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    • 1999
  • Psoriasis is the recurrent disease of skin and till now its mechanism, pathogenesis and treatment are not clearly discovered. So, like these papers, we must investigate more safety and effective treatment of psoriasis. And I obtained the following condusions from the Oriental and Occidental bibliographic investigation. 1. In Oriental medicine. Baikbi, Pungsun, Eunselbyong, Songpisun, Baikselpung were the other names of Psoriasis. 2. In Occidental medicine, genetic factors, aggravation and inducible factors, biochemical factors, immunologic factors, disorder of epidermal kinetics, vascular malformation of dermis were cosidered to the pathogenic factors of psoriasis. 3. In Oriental medicine, the pathogenic factors of psoriasis were divided two parts. One is exogenous pathogenic factors which including the blood-heat, blood dryness, blood stasis, deficiency of liver and kidney, inharmony of impulsive and appointed pulsation, deficiency of blood. The other is exogenous pathogenic factors which including the wind-evil, wind-wetness, wind-heat, wind-cold, wetness-heat, cold-wetness, heat-evil. 4. In Occidental medicine, external applicative medicine, internal medicine, ultraviolet therapy, ultraviolet - external applicative medicine compound therapy and etc. were used the therapy of psoriasis. 5. In Oriental medicine, clean away heat and cooling blood, aliment the blood and moisturize, activating blood and expelling blood stasis, harmonize and invigorate the liver and kidney, invigorate the kidney, aliment the blood and moisturize the skin, aliment the blood and expelling the wind, expelling the wind and wetness, clean away heat and expelling wind, expelling the wind and scatter the cold, clean away heat and expelling the wetness, heat up the meridian and scatter the cold, clean away heat and remove the toxin and etc. were used the method of internal therapy of psoriasis. 6. Until Qing dynasty, wind expelling effective prescriptions like Bangpungtongsungsan, Sopungsan. Supungsunkisan and etc. were used and recently Yanghyelgeupungtang, Hwalhyelgeupungtang, Samultanggagam and etc. were used the internal therapy of psoriasis. 7. In the external therapy of psoriasis, Yuhonggo, Pungyugo, Sekryupiyeongo were used the plaster therapy and Folium Rerillae, Camphora, Fructus, Xanthii, Herba Spirodelae compound prescription were used the cleansing therapy, Okgisan, Chiunsan, Galmyogo, Hobunsan, Muisan, Madugo, Jeyugohengin were used the rubbing skin therapy. Rangdok Radix Aconiti, Bijeonilchoalkwang were used the attaching therapy, the extract of Rhizoma Et Radix Veratri was used the spreading pouder therapy. 8. In the acupuncture therapy of psoriasis, the Jelgol, chok Samni(S36), Kansa(P5), Haegye(S41), Wijung(B40) and etc. were used the acupuncture point, and the angle of helix incision threapy that disinfect and cut the angle of helix and plaster the Semen Glycine and Squama Manitis were used.

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환자 맞춤형 전자기장 요실금 치료 시스템 개발 (Development of Individually Adapted Electromagnetic Therapy System in Incontinence)

  • 노시철;강규홍;이정석;민권식;권장우;최흥호
    • 전자공학회논문지SC
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    • 제45권4호
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    • pp.51-59
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    • 2008
  • 요실금은 자신의 의지와는 무관하게 소변이 나오는 배뇨이상으로 삶의 질을 떨어뜨리는 대표적인 여성 질환이다. 치료방법 중 자기장을 이용한 치료는 자발적인 운동을 요구하지 않고, 수치심을 유발하지 않으며, 감염의 위험이 없기 때문에 가장 높은 발전 가능성을 가지고 있다. 하지만 획일화된 치료 프로토콜, 환자 의존적 치료방법, 환자 상태 평가 부재 등의 개선점도 갖고 있다. 이에 본 연구에서는 자기장을 이용하여 골반저근을 자극하고, 치료 중 환자의 상태 변화를 실시간 모니터링하는 시스템을 제안하였다. 또한, 환자의 요실금 정도에 따른 환자 맞춤형 자기장 치료 시스템을 제안하였다. 제안된 시스템은 자기장 발생장치, 냉각장치, 치료의자, 맥박 모니터링을 이용한 환자 감시 장치, 제어 소프트웨어로 구성되었으며, 시뮬레이션을 통한 고전력 시스템 설계 과정과 개발 후 성능 평가를 수행하였다. 제어 소프트웨어 개발을 통하여 사용의 편의성과 관리의 편의성을 확보하였으며, 환자의 증상별 치료 프로토콜을 적용하였다. 본 연구를 통하여 개발된 환자 모니터링을 포함한 환자 맞춤형 자기장 치료 시스템은 자기장 치료에서 발생할 수 있는 위험성을 줄이고, 환자의 증상에 맞게 패턴화된 치료법을 제시함으로써 요실금 자기장 치료의 효율성을 높일 수 있고, 전/후 치료를 통하여 환자에게 보다 접근된 치료법이라고 판단되었다. 이러한 결과는 요실금 환자들에게 보다 안전하고 체계적인 치료법을 제공하며, 요실금 자기장 치료 방법의 다양한 연구를 유도할 수 있을 것으로 사료되었다.

거대자기저항 스핀밸브 소자를 이용한 의료용 냉각기 수위 및 수온 센서의 제작과 특성 (Fabrication and Property of Water Level and Temperature Sensor for Medical Cooling System Using a Highly Sensitive GMR-SV Device)

  • 박광준;최종구;이상석;이범주
    • 한국자기학회지
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    • 제21권1호
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    • pp.32-36
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    • 2011
  • DC 마그네트론 스퍼터링을 이용하여 제작한 고감도 거대자기저항 스핀밸브 박막 소자를 이용하여 수위 및 수온을 측정할 수 있는 센서를 제작하였다. 제작한 센서의 수위 및 수온 분해능은 각각 $400\;m{\Omega}/mm$$100\;m{\Omega}/^{\circ}C$ 이었으며, 이는 전립선 비대증 KTP(potassium titanyl phosphate) 레이저 치료기에서 냉각기의 수위 및 수온을 조절하는 센서로써 응용이 가능하다.

조직모델에서 냉.온치료기의 열전달 특성 비교 연구 (A Comparative Study on Heat Transfer Characteristics in Tissue Model with Application of Heating or Cooling Therapeutic Modalities)

  • 김유종;신경민;김은정;김경호;김갑성;이승덕
    • Journal of Acupuncture Research
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    • 제30권4호
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    • pp.125-138
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    • 2013
  • Objectives : This study aimed to investigate temperature characteristics by heat transfer type of therapeutic modalities. Methods : We selected heating and cooling modalities that are frequently used in clinical by heat transfer type: conduction, convection, radiation, and conversion. We used ham as tissue model, and applied the modalities for 30 minutes. We measured real-time changed temperature($^{\circ}C$) of the surface, 2, 4, 6, 8, 10, 12, and 14 mm depth. Results : 1. In conduction-using hot pack, ice pack, and CryoStamp heating-cooling combination therapy unit($40^{\circ}C/{\sim}15^{\circ}C$), the surface temperature sharply rose close to equilibrium in first 5 minutes. 2. In convection-using smokeless moxa, temperature slowly rose to the maximum at 25-minute elapsed time. But in another convection-using CRAiS cryotherapy device, result was similar to that of conduction. 3. In radiation-using infrared lamp, result was similar to that of conduction, but not reached equilibrium during applying time. 4. In conversion-using ultrasound device, temperature was the highest at 6 mm depth, and not reached equilibrium during applying time. Conclusions : We could comprehend temperature characteristics and proper use of modalities by heat transfer type. It would be necessary to consider in vivo physical conditions in further studies.

경추부 후면 및 측면 피부 냉각 작용이 대학생의 주의력에 미치는 영향 (The Effect on Attention of College Students by Epidermal Cooling in Posterior and Lateral of Upper Cervix)

  • 장지홍
    • 한국정보전자통신기술학회논문지
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    • 제15권5호
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    • pp.328-334
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    • 2022
  • 사람의 인지기능 중 주의력은 감각기관을 통해 방대한 자극 중 필요한 자극에만 의식을 집중하는 과정이다. 주의력은 실내 온도 등 노출 환경에 영향을 받는 것으로 알려져 있다. 스포츠 분야에서는 냉각 팩 등을 사용한 직접 전도 방식의 냉각이 광범위하게 사용되어 효과를 보고 있으나 전도에 의한 냉각이 주의력과 같은 인지 활동에 미치는 영향에 대해서는 알려진 바가 없다. 본 연구에서는 경추부 후면 부위에 특수 제작된 냉각 패드를 적용할 때 주의력에 미치는 영향을 살펴보기 위해 수행되었다. 40명의 피검사자를 네 집단으로 나누고 독서 및 산책 중 냉각 패드의 적용에 따라 주의력이 차이를 보이는지 살펴보았다. 주의력은 FAIR 주의력 검사를 통해 평가되었으며, 선택적 주의력을 의미하는 능력지수, 주의력의 정확도를 나타내는 통제지수, 균일한 주의력의 유지 기간을 나타내는 지속성지수에 대한 분산분석과 사후분석을 실시하였다. 결과적으로, 독서 및 학습 등 시각적 주의력이 지속적으로 요구되는 경우 냉각 패드의 적용을 통한 전도 냉각이 선택적 주의력을 확대시키고 균일한 주의력을 유지하는데 도움이 된다. 또한, 선택적 주의력의 향상과 균일한 주의력의 유지를 위해 가벼운 신체 활동이 도움이 된다. 다만 이 경우 전체 독서 시간의 감소를 전제로 하여야 한다.

프로 골프 선수의 운동 스트레스, 긴장도, 운동 습관과 스포츠 손상과의 관계 (Relationship between Exercise Stress, Tension, Exercise Habits and Sports Injuries of Professional Golf Players)

  • 서동익;최병선;서경묵
    • 대한물리치료과학회지
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    • 제19권4호
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    • pp.17-25
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    • 2012
  • Purpose : The purpose of this study is to know sports injury aspects and relationship between exercise stress, tension, exercise habits and sports injuries. Methods : We surveyed 42 professional golf players who attended Korea Tour during the second half of the year 2010 and have sports injuries with self - assessment questionnaires. Results : According to the types, they had myalgia, tendonitis, ligament sprain in the order. According to the body parts, they had injuries in right thoracic, left cervical, left shoulder region in the order. As a result of the analysis of the sports injury frequency according to the exercise stress and tension, there were no significant differences between the high and low level group. But result of injury regions showed high tension level group showed more damages in right thoracic region (p<0.05). Exercise habits appear subjects had lots of training time, however, they used to skip the muscular strengthening exercise during the on season, but had no significant difference between sports injury frequencies and exercise habit. Conclusion : For the improvement of the athletic performance and prevention of the sports injury, warming-up, cooling-down and muscular strengthening exercise program developments are needed.

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