• Title/Summary/Keyword: Cooling Therapy

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A New Protocol for Effective Cryopreservation of Human Embryonic Stem Cells by a Minimum Volume Cooling Method

  • Kim, Eun-Young;Lee, Keum-Sil;Shin, Hyun-Ah;Park, Sae-Young;Yoon, Ji-Yeon;Kil, Kwang-Soo;Lee, Young-Jae;Kim, Nam-Hyung;Chung, Kil-Saeng
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 2003.10a
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    • pp.98-98
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    • 2003
  • Recently, human embryonic stem (hES) cells have become very important resources for ES cell basic research, cell replacement therapy, and other medical applications; thus, efficient cryopreservation methods for these cells are needed. This study examined whether a newly developed minimum volume cooling (MVC) vitrification method, which was tested through cryopreservation of sensitive bovine oocytes, can be used for freezing hES cells. Feeder-free cultured hES cell (MB03) colonies were mechanically dissected into several small clumps following enzymatic treatment. We compared the freezing efficiency of a slow-cooling method using a cryo-module (0.4-0.6C/min, 20-30 clumps/vial) and MVC vitrification using a modified 0.5-ml French mini-straw designated as a MVC straw (>$20,000{\circ}C$/min, 10 clumps/straw) After thawing, in vitro survival of hES cell clumps was higher for MVC-vitrified cells (80.8%, 97/120) than for slow-cooled cells (38.2%, 39/102). Further, the proliferation rate of surviving MVC-vitrified cells was similar to that of control hES cells from 2 weeks after thawing. In addition, vitrified-thawed hES cells demonstrated a normal karyotype, were positively immunostained for surface marker antibodies (AP, SSEA-4 and TRA-1-60) and the Oct-4 antibody, and could differentiate into all three embryonic germ layer cells in vitro. This result demonstrates that hES cell clumps can be successfully cryopreserved by a newly developed MVC vitrification method without loss of human cell characteristics.

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A literatual studies on the ajangpung(鵝掌風). (鵝掌風에 關한 文獻的 考察)

  • Jang, Gi-young;Roh, Sek-seon
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.11 no.1
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    • pp.141-161
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    • 1998
  • In the literatual studies on the ajangpung(鵝掌風), the results were as follows. Ajangpung(鵝掌風) is called 'Jangsimpung(掌心風)', is concerned with 'Tinea Manus(手癬)' in western medicine. The pathogenic factors of Ajangpung were stomach-heat & blood dryness, wind-cold-wetness evil, or contact infection etc. The treatments of Ajangpung were used external treatments for dispelling wind-evil & cooling blood, dispelling wetness-evil & parasiticidal therapy. In the frequency of prescription, the most numerous prescription was Yibansan(二礬散) and the next were Yungigo(潤肌膏), Gupungjihwanghwan(祛風地黃丸) etc.

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Bilateral Skin Temperature Change of the Anterior Thigh Following Unilateral Isokinetic Exercise (등속성 운동 후 양측 대퇴부의 피부 온도 변화)

  • Kim, Seon-Mi;Oh, Young-Soo;Lee, Ji-Eun;Kwon, Hyuk-Cheol
    • Physical Therapy Korea
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    • v.2 no.1
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    • pp.14-20
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    • 1995
  • The purpose of this study was to measure and compare the skin temperature over the exercised muscle and corresponding non-exercised muscle after unilateral isokinetic exercise using digital thermography. Thirty-two young healthy volunteers with no history of knee injury were tested. After isokinetic exercise at 60 degree per second angular velocity using the right leg in a climatic chamber at ambient temperature of $23-26^{\circ}C$, skin temperature of the anterior thigh was tested. After exercise, the skin temperature of both the right and left leg had fallen significantly. The skin temperature of the exercised leg fell less than that of the non-exercised leg. The fall in skin temperature after work was not due to increased evaporative cooling, but was the result of segmental vasoconstriction probably caused reflexly in the spinal cord by non-thermal afferents from exercising muscle or moving tissues. The effect of thermoregulatory vasodilation was reduced by reflex vasoconstriction caused by non-thermal factors such as catecholamine.

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CURRENT RESEARCH ON ACCELERATOR-BASED BORON NEUTRON CAPTURE THERAPY IN KOREA

  • Kim, Jong-Kyung;Kim, Kyung-O
    • Nuclear Engineering and Technology
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    • v.41 no.4
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    • pp.531-544
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    • 2009
  • This paper is intended to provide key issues and current research outcomes on accelerator-based Boron Neutron Capture Therapy (BNCT). Accelerator-based neutron sources are efficient to provide epithermal neutron beams for BNCT; hence, much research, worldwide, has focused on the development of components crucial for its realization: neutron-producing targets and cooling equipment, beam-shaping assemblies, and treatment planning systems. Proton beams of 2.5 MeV incident on lithium target results in high yield of neutrons at relatively low energies. Cooling equipment based on submerged jet impingement and micro-channels provide for viable heat removal options. Insofar as beam-shaping assemblies are concerned, moderators containing fluorine or magnesium have the best performance in terms of neutron accumulation in the epithermal energy range during the slowing-down from the high energies. NCT_Plan and SERA systems, which are popular dose distribution analysis tools for BNCT, contain all the required features (i.e., image reconstruction, dose calculations, etc.). However, detailed studies of these systems remain to be done for accurate dose evaluation. Advanced research centered on accelerator-based BNCT is active in Korea as evidenced by the latest research at Hanyang University. There, a new target system and a beam-shaping assembly have been constructed. The performance of these components has been evaluated through comparisons of experimental measurements with simulations. In addition, a new patient-specific treatment planning system, BTPS, has been developed to calculate the deposited dose and radiation flux in human tissue. It is based on MCNPX, and it facilitates BNCT efficient planning based via a user-friendly Graphical User Interface (GUI).

The Oriental and Occidental bibliographic Study of Psoriasis (乾癬에 對한 東.西醫 文獻的 硏究)

  • Kim, Nam-Kwen;Hwang, Chung-yeon;Lim, Gye-sang
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.12 no.1
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    • pp.154-178
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    • 1999
  • Psoriasis is the recurrent disease of skin and till now its mechanism, pathogenesis and treatment are not clearly discovered. So, like these papers, we must investigate more safety and effective treatment of psoriasis. And I obtained the following condusions from the Oriental and Occidental bibliographic investigation. 1. In Oriental medicine. Baikbi, Pungsun, Eunselbyong, Songpisun, Baikselpung were the other names of Psoriasis. 2. In Occidental medicine, genetic factors, aggravation and inducible factors, biochemical factors, immunologic factors, disorder of epidermal kinetics, vascular malformation of dermis were cosidered to the pathogenic factors of psoriasis. 3. In Oriental medicine, the pathogenic factors of psoriasis were divided two parts. One is exogenous pathogenic factors which including the blood-heat, blood dryness, blood stasis, deficiency of liver and kidney, inharmony of impulsive and appointed pulsation, deficiency of blood. The other is exogenous pathogenic factors which including the wind-evil, wind-wetness, wind-heat, wind-cold, wetness-heat, cold-wetness, heat-evil. 4. In Occidental medicine, external applicative medicine, internal medicine, ultraviolet therapy, ultraviolet - external applicative medicine compound therapy and etc. were used the therapy of psoriasis. 5. In Oriental medicine, clean away heat and cooling blood, aliment the blood and moisturize, activating blood and expelling blood stasis, harmonize and invigorate the liver and kidney, invigorate the kidney, aliment the blood and moisturize the skin, aliment the blood and expelling the wind, expelling the wind and wetness, clean away heat and expelling wind, expelling the wind and scatter the cold, clean away heat and expelling the wetness, heat up the meridian and scatter the cold, clean away heat and remove the toxin and etc. were used the method of internal therapy of psoriasis. 6. Until Qing dynasty, wind expelling effective prescriptions like Bangpungtongsungsan, Sopungsan. Supungsunkisan and etc. were used and recently Yanghyelgeupungtang, Hwalhyelgeupungtang, Samultanggagam and etc. were used the internal therapy of psoriasis. 7. In the external therapy of psoriasis, Yuhonggo, Pungyugo, Sekryupiyeongo were used the plaster therapy and Folium Rerillae, Camphora, Fructus, Xanthii, Herba Spirodelae compound prescription were used the cleansing therapy, Okgisan, Chiunsan, Galmyogo, Hobunsan, Muisan, Madugo, Jeyugohengin were used the rubbing skin therapy. Rangdok Radix Aconiti, Bijeonilchoalkwang were used the attaching therapy, the extract of Rhizoma Et Radix Veratri was used the spreading pouder therapy. 8. In the acupuncture therapy of psoriasis, the Jelgol, chok Samni(S36), Kansa(P5), Haegye(S41), Wijung(B40) and etc. were used the acupuncture point, and the angle of helix incision threapy that disinfect and cut the angle of helix and plaster the Semen Glycine and Squama Manitis were used.

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Development of Individually Adapted Electromagnetic Therapy System in Incontinence (환자 맞춤형 전자기장 요실금 치료 시스템 개발)

  • Noh, Si-Cheol;Kang, Kyu-Hong;Lee, Jeong-Seok;Min, Kwon-Sik;Kwon, Jang-Woo;Choi, Heung-Ho
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.45 no.4
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    • pp.51-59
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    • 2008
  • Incontinence is the urination disorder as the leakage of urine without her own volition and the woman's representative disease which reduce the life quality. The electromagnetic therapy has high possibility of development cause it has no needs of operant exercise, no arousing of shame and impossibility of infection. But, it has improvement points such as uniformity of the treatment protocol, patient dependance and absent of patient monitoring system. With these demands, the system which stimulate the pelvic flaw muscle with electromagnetic and monitoring the patient status during the therapy is proposed, in this study. And individually adapted electromagnetic therapy system for incontinence patient is also suggested. The proposed system consisted of electromagnetic generation device, cooling device, treating chair, patient monitoring device with pulsation and control software. The simulation for high power system and evaluation confirm was performed. With the development of control software, the convenience of using and maintenance are ensured and the patient adapted therapy protocol is applied. The developed patient adapted electromagnetic therapy system with monitoring device is regarded as the patient affinitive treating method by reducing the riskiness, improving the efficiency with patterned protocol and pre/post therapy. These results, in this study, can bring the safe and organized treatment method to incontinence patients and can lead the variable study for electromagnetic therapy in incontinence.

Fabrication and Property of Water Level and Temperature Sensor for Medical Cooling System Using a Highly Sensitive GMR-SV Device (거대자기저항 스핀밸브 소자를 이용한 의료용 냉각기 수위 및 수온 센서의 제작과 특성)

  • Park, Kwang-Jun;Choi, Jong-Gu;Lee, Sang-Suk;Lee, Bum-Ju
    • Journal of the Korean Magnetics Society
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    • v.21 no.1
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    • pp.32-36
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    • 2011
  • We fabricated a sensor for measuring the water level and water temperature using GMR-SV (giant magnetoresistance-spin valve) device, simultaneously. It could be applied to the medical cooling system of the potassium titanylphosphate KTP) laser system for the therapy of a benign prostatic hyperplasia. The middle point of GMR-SV device was set to the near position of a high magnetic sensitivity with 2.8%/Oe. The sensitivity for the water level and water temperature of the fabricated sensor were $400\;m{\Omega}/mm$ and $100\;m{\Omega}/^{\circ}C$, respectively.

A Comparative Study on Heat Transfer Characteristics in Tissue Model with Application of Heating or Cooling Therapeutic Modalities (조직모델에서 냉.온치료기의 열전달 특성 비교 연구)

  • Kim, Yu Jong;Shin, Kyung Min;Kim, Eun Jung;Kim, Kyung Ho;Kim, Kap Sung;Lee, Seung Deok
    • Journal of Acupuncture Research
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    • v.30 no.4
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    • pp.125-138
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    • 2013
  • Objectives : This study aimed to investigate temperature characteristics by heat transfer type of therapeutic modalities. Methods : We selected heating and cooling modalities that are frequently used in clinical by heat transfer type: conduction, convection, radiation, and conversion. We used ham as tissue model, and applied the modalities for 30 minutes. We measured real-time changed temperature($^{\circ}C$) of the surface, 2, 4, 6, 8, 10, 12, and 14 mm depth. Results : 1. In conduction-using hot pack, ice pack, and CryoStamp heating-cooling combination therapy unit($40^{\circ}C/{\sim}15^{\circ}C$), the surface temperature sharply rose close to equilibrium in first 5 minutes. 2. In convection-using smokeless moxa, temperature slowly rose to the maximum at 25-minute elapsed time. But in another convection-using CRAiS cryotherapy device, result was similar to that of conduction. 3. In radiation-using infrared lamp, result was similar to that of conduction, but not reached equilibrium during applying time. 4. In conversion-using ultrasound device, temperature was the highest at 6 mm depth, and not reached equilibrium during applying time. Conclusions : We could comprehend temperature characteristics and proper use of modalities by heat transfer type. It would be necessary to consider in vivo physical conditions in further studies.

The Effect on Attention of College Students by Epidermal Cooling in Posterior and Lateral of Upper Cervix (경추부 후면 및 측면 피부 냉각 작용이 대학생의 주의력에 미치는 영향)

  • Chang, Ji Hong
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.15 no.5
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    • pp.328-334
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    • 2022
  • The process that one may consciously focuses on necessary stimulation among tremendous amount of stimulation through human sensory systems is called attention in psychology. It is known that the attention can be affected by many factors such as room temperatures, humidity level, etc. In the field of sports science, ice packs are widely used for recovery from exercise fatigue providing fast heat transfer by conduction. However, the effect on attention by so-called iced-pack-cooling has not been tested. This research focuses on the attention levels when one is provided with a special cooling pad on their dorsal and lateral cervices. 40 subjects were divided into four groups and their attention level was evaluated based on the exposure conditions of combinations in reading and light walking with and without the cooling pad. The Frankfruter Aufmerksamkeits-Inventar, FAIR was used to evaluate the attention levels; the performance index, quality index, and continuity index consist of the FAIR test indicating the selectiveness of the attention, correctness of the attention, and maintaining term of the attention, respectively. Analysis of variance was carried out for those variables and post-hoc if applicable. When visual attention is constantly used for reading and studying, application of conductive heat transfer by the cooling pads is significantly helpful for improvement in selectiveness of the attention and maintaining terms of the attention levels. Also, light walking yielded improvement in selectiveness of the attention and maintaining terms of the attention levels; however one should presupposedly consider the loss of reading time.

Relationship between Exercise Stress, Tension, Exercise Habits and Sports Injuries of Professional Golf Players (프로 골프 선수의 운동 스트레스, 긴장도, 운동 습관과 스포츠 손상과의 관계)

  • Seo, Dong-Ick;Choi, Byung-Sun;Seo, Kyung-Mook
    • Journal of Korean Physical Therapy Science
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    • v.19 no.4
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    • pp.17-25
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    • 2012
  • Purpose : The purpose of this study is to know sports injury aspects and relationship between exercise stress, tension, exercise habits and sports injuries. Methods : We surveyed 42 professional golf players who attended Korea Tour during the second half of the year 2010 and have sports injuries with self - assessment questionnaires. Results : According to the types, they had myalgia, tendonitis, ligament sprain in the order. According to the body parts, they had injuries in right thoracic, left cervical, left shoulder region in the order. As a result of the analysis of the sports injury frequency according to the exercise stress and tension, there were no significant differences between the high and low level group. But result of injury regions showed high tension level group showed more damages in right thoracic region (p<0.05). Exercise habits appear subjects had lots of training time, however, they used to skip the muscular strengthening exercise during the on season, but had no significant difference between sports injury frequencies and exercise habit. Conclusion : For the improvement of the athletic performance and prevention of the sports injury, warming-up, cooling-down and muscular strengthening exercise program developments are needed.

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