• Title/Summary/Keyword: Conway cell microdiffusion

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ELIMINATION PATTERNS OF ARTERIAL BLOOD CYANIDE ION IN THIOSULFATE-OXYGEN ADMINISTERED RABBIT

  • Yoo, Keun-Young;Lee, Yoon-Seong;Yun, Dork-Ro
    • Toxicological Research
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    • v.3 no.2
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    • pp.89-96
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    • 1987
  • To test the efficacies of thiosulfate in cyanide poisoning with or without oxygen, after the administration of sublethal dose of potassium cyanide, serial arterial blood samples were collected during 60 minutes in 15 rabbits. Cyanide ion concentrations were measured by Conway cell microdiffusion method, and arterial oxygen tensions were also observed. Comparison of elimination constants showed that arterial blood cyanide ion concentration decreased most rapidly in the thiosulfate with oxygen-administered group. The elimination of cyanide ion by the action of thiosulfate in acutely poisoned rabbit accelerated probably due to oxygen and elimination pattern seems to occur by first-order elimination kinetics.

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Ammonia Microdiffusion and Colorimetic Method for Determining Nitrogen in Plant Tissues (암모니아 확산 및 발생에 의한 식물조직의 질소분석 방법)

  • Tae-Hwan Kim;Byung Ho-Kim
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.16 no.4
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    • pp.253-259
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    • 1996
  • Ammonia microdiffusion method and colorimetric measurement are described for the nitrogen determination. The diffusion of ammonia could be successfully induced by using a microdiffusion cell. It is a simple and rapid technique, which is suitable for transforming the nitrogen in digests into $NH_4CI$ for the colorimetric N determination with ammonia color reagent. Above 99% of N recovery were obtained with microdiffusion up to 15 hours. The coloration method of collected $NH_4CI$ for the colorimetric N determination was also estabilshed with a scanning in U.V. spectrophotometer. Under the proposed coloration method (0.5 mL of sample digest, 4.0 mL of $H_2O$ and 0.5 mL of ammonia color reagent), a maximal absorbance was observed at 410 nm. The kinetic measurement of absorbance showed a high stability from 5 to 45 minutes after color development. Absorbance was directly proportional to the amount of $NH_4^+-N$ present. The microdiffusion-ammonia coloration method was successfully applied to the nitrogen determination in the forms of protein-N or total -N in plant tissue. Comparing with Kjeldahl distillation method, the values obtained with described method were slightly higher and more reliable.

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