• 대한화학회지
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• 제41권9호
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• pp.483-487
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• 1997

Chromobacterium chocolatum의 whole cell을 polyacrylamide의 matrix안에 고정화 하였으며 이를 이용하여 dimethyl-cis-1,3-dibenzyl-2-oxoimidazolidine-4,5-dicarboxlyate를 가수분해 하였다. 고정화된 mycelium을 이용하여 가수분해하는 최적조건을 연구하였다. 기질의 농도, 반응시간, 용액의 pH변화에 다른 lipase의 안정성및 반응의 영향을 연구하였다. 고정회된 mycelium의 리파아제의 활동도는 4주이상 지속되었고 wet cell 2g을 고정화 하여 200 mg의 기질을 pH=7의 반응조건에서 반응시켰을 때 최대 수율로 생성물이 얻어졌다.

• 간호행정학회지
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• 제10권1호
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• pp.121-140
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• 2004

Purpose: This study is to analyse and observe the college of oriental medicine related with nursing investment contents, its conversion process and output to get good qualified oriental nursing result, thus developing a matrix of oriental nursing management system on the basis of that project. Method: This study was nursing management system of matrix developmental study for Korea was performed to the objectives of 11 college of oriental medicine nursing directors and 328 nurses with more than three-year experiences in Korea. Result: We found difficulties to utilize knowledge obtained through the regular curriculum to the practice of oriental nursing because only me or two subjects were running or even no syllabus. Therefore, we recommend that oriental nursing in-service education program as well as oriental nursing practice preceptorship should be administered en site. The evaluation of oriental nursing and its feedback, duty related multiple evaluation, aggressive QI activity and feedback of the performance evaluation oriental nurse's role and duty manual also should be essentially organized. Conclusion: We urgently request that the consensus of nursing school and nurse association to be made about oriental nursing activity as a basic curriculum of compulsive subject.

• 한국지능시스템학회:학술대회논문집
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• 한국퍼지및지능시스템학회 2005년도 추계학술대회 학술발표 논문집 제15권 제2호
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• pp.95-98
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• 2005

This paper presents intelligent digital redesign method of global approach for hybrid state space fuzzy-model-based controllers. For effectiveness and stabilization of continuous-time uncertain nonlinear systems under discrete-time controller, Takagi-Sugeno(TS) fuzzy model is used to represent the complex system. And global approach design problems viewed as a convex optimization problem that we minimize the error of the norm bounds between nonlinearly interpolated linear operators to be matched. Also by using the power series, we analyzed nonlinear system's uncertain parts more precisely. When a sampling period is sufficiently small, the conversion of a continuous-time structured uncertain nonlinear system to an equivalent discrete -time system have proper reason. Sufficiently conditions for the global state -matching of the digitally controlled system are formulated in terms of linear matrix inequalities (LMls). Finally, we prove the effectiveness and stabilization of the proposed intelligent digital redesign method by applying the chaotic Lorentz system.

• 대한기계학회논문집
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• 제18권10호
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• pp.2554-2566
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• 1994

Robot position/force control has been a difficult task owing to the interaction between a robot and an environment with a rather high stiffness. In addition to the dynamic instability, the interaction causes the following problem : 1) chattering at steady-state, 2) dynamic coupling effect of robot, and 3) performance degradation due to a titled environment. To solve the problem, the Time Delay Control(TDC), which has been known to be quiet robust to plant uncertainties and disturbances, has been applied. In conjunction to TDC, the following three ideas were also used : 1) To reduce the amplitude of the chattering at the steady state, a novel scheme was adopted to enhance the resolution type solution of A/D conversion for the force sensor. 2) To reduce the dynamic coupling, a trajectory type position command was tried on a comparative basis to the step command, as well as a more accurate mass matrix was used instead of the constant mass matrix. 3) And finally to improve the performance in the tilted environment, force derivatives instead of position derivatives were used in the TDC law. Computer simulations and experiments resulted in obvious improvements on the quality of the hybrid control, thereby clearly demonstrating the effectiveness of TDC with the proposed ideas.

• Tuberculosis and Respiratory Diseases
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• 제71권3호
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• pp.172-179
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• 2011

Background: Statins can regulate the production of pro-inflammatory cytokines and inhibit MMP production or activation in a variety of types of cells. This study evaluated whether statins would inhibit MMP release from human lung fibroblasts, which play a major role in remodeling processes. Methods: This study, using an in-vitro model (three-dimensional collagen gel contraction system), evaluated the effect of cytokines (tumor necrosis factor-${\alpha}$, TNF-a and interleukin-$1{\beta}$, IL-1b) on the MMP release and MMP activation from human lung fibroblasts. Collagen degradation induced by cytokines and neutrophil elastase (NE) was evaluated by quantifying hydroxyproline. Results: In three-dimensional collagen gel cultures (3D cultures) where cytokines (TNF-a and IL-1b) can induce the production of MMPs by fibroblasts, it was found that simvastatin inhibited MMP release. In 3D cultures, cytokines together with NE induced collagen degradation and can lead to activation of the MMP, which was inhibited by simvastatin. Conclusion: Simvastatin may play a role in regulating human lung fibroblast functions in repair and remodeling processes by inhibiting MMP release and the conversion from the latent to the active form of MMP.

• 전력전자학회:학술대회논문집
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• 전력전자학회 2008년도 하계학술대회 논문집
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• pp.106-108
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• 2008

This paper deals with the design for speed controller to drive PMSM by matrix converter without DC-link circuit as the power conversion system of AC servo motor drive. To design the speed controller of PMSM drive, the closed-loop transfer function of speed controller is calculated and then the frequency-domain response characteristics are analyzed by bode plot using Matlab. Based on the results by bode plot, the speed control gains are determined. As the real effects of controller designed in the frequency-domain display in the time-domain, the performance of speed controller is confirmed by the step response of speed controller. The design examples are shown and its validity of the design method mentioned in the paper is verified through PSIM simulation.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2000년도 하계학술대회 논문집 B
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• pp.635-637
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• 2000

To analyze the transient state of an induction motor, there have been studies for using the magnetic equivalent circuit method (MECM) instead of the time differential finite-element method, MECM which analyzes magnetic equivalent circuits after converting each part of an electric machine into the magnetic circuit elements. has the merits of short calculation-time and comparatively accurate results. To analyze an electric machine with MECM, we have to replace stator and rotor with the magnetic elements and express the air gap, where electromechanical energy conversion takes place, with the permeance. So in this paper, to analyze an Induction Motor with MECM, we express the magnetic equivalent circuit as algebraic equations and then as the matrix for solving easily them.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2000년도 추계학술대회 논문집 학회본부 B
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• pp.330-332
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• 2000

To analyze the transient state of an induction motor, there have been studies for using the magnetic equivalent circuit method(MECD) instead of the time differential finite-element method. MECD which analyzes magnetic equivalent circuits after converting each part of an electric machine into the magnetic circuit elements, has the merits of short calculation-time and comparatively accurate results. To analyze an electric machine with MECM, we have to replace stator and rotor with the magnetic elements and express the air gap, where electromechanical energy conversion takes place, with the permeance. So in this study, to analyze an Induction Motor with HECM, we express the magnetic equivalent circuit as algebraic equations and then as the matrix for solving easily them. In particular, all relations are formed with matrixes to solve Mathematically them in the programming process later. As a result, this theory will be the basis on the static and dynamic analysis of an Induction Motor.

• Mass Spectrometry Letters
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• 제12권2호
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• pp.47-52
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• 2021

Evaluating the impurity concentrations in semiconductor thin films using time of flight secondary ion mass spectrometry (ToF-SIMS) is an effective technique. The mass interference between isotopes and matrix element in data interpretation makes the process complex. In this study, we have investigated the doping concentration of phosphorus in, phosphorus doped silicon thin film on glass using ToF-SIMS in the dynamic mode of operation. To overcome the mass interference between phosphorus and silicon isotopes, the quantitative analysis of counts to concentration conversion was done following two routes, standard relative sensitivity factor (RSF) and SIMetric software estimation. Phosphorus doped silicon thin film of 180 nm was grown on glass substrate using hot wire chemical vapor deposition technique for possible applications in optoelectronic devices. Using ToF-SIMS, the phosphorus-31 isotopes were detected in the range of 10¹~10⁴ counts. The silicon isotopes matrix element was measured from p-type silicon wafer from a separate measurement to avoid mass interference. For the both procedures, the phosphorus concentration versus depth profiles were plotted which agree with a percent difference of about 3% at 100 nm depth. The concentration of phosphorus in silicon was determined in the range of 10¹⁹~10²¹ atoms/cm³. The technique will be useful for estimating distributions of various dopants in the silicon thin film grown on glass using ToF-SIMS overcoming the mass interference between isotopes.

• Tuberculosis and Respiratory Diseases
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• 제77권2호
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• pp.73-80
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• 2014

Background: Low levels of serum vitamin D is associated with several lung diseases. The production and activation of matrix metalloproteinases (MMPs) may play an important role in the pathogenesis of emphysema. The aim of the current study therefore is to investigate if vitamin D modulates the expression and activation of MMP-2 and MMP-9 in human lung fibroblasts (HFL-1) cells. Methods: HFL-1 cells were cast into three-dimensional collagen gels and stimulated with or without interleukin-$1{\beta}$ (IL-$1{\beta}$) in the presence or absence of 100 nM 25-hydroxyvitamin D (25(OH)D) or 1,25-dihydroxyvitamin D ($1,25(OH)_2D$) for 48 hours. Trypsin was then added into the culture medium in order to activate MMPs. To investigate the activity of MMP-2 and MMP-9, gelatin zymography was performed. The expression of the tissue inhibitor of metalloproteinase (TIMP-1, TIMP-2) was measured by enzyme-linked immunosorbent assay. Expression of MMP-9 mRNA and TIMP-1, TIMP-2 mRNA was quantified by real time reverse transcription polymerase chain reaction. Results: IL-$1{\beta}$ significantly stimulated MMP-9 production and mRNA expression. Trypsin converted latent MMP-2 and MMP-9 into their active forms of MMP-2 (66 kDa) and MMP-9 (82 kDa) within 24 hours. This conversion was significantly inhibited by 25(OH)D (100 nM) and $1,25(OH)_2D$ (100 nM). The expression of MMP-9 mRNA was also significantly inhibited by 25(OH)D and $1,25(OH)_2D$. Conclusion: Vitamin D, 25(OH)D, and $1,25(OH)_2D$ play a role in regulating human lung fibroblast functions in wound repair and tissue remodeling through not only inhibiting IL-$1{\beta}$ stimulated MMP-9 production and conversion to its active form but also inhibiting IL-$1{\beta}$ inhibition on TIMP-1 and TIMP-2 production.