• 대한미생물학회지
• /
• 제22권4호
• /
• pp.445-451
• /
• 1987

Coagglutination method is widely used for the diagnosis of Salmonella infection. This test, however, has a disadvantage of false positive reaction due to the coagglutination of staphylococci with non-specific immune complexes or anti-staphylococci antibody in serum. Salmonell O antigen was detected by enzyme immunoassay with protein A-bearing Staphylococcus aureus as in the solid phase. Horse radish peroxidase was labeled to IgG specific against Salmonella O antigen. This enzyme immunoassay was much more sensitive than conventional coagglutination method without false poitive agglutination. To improve the sensitivity for detection of Salmonella O antigen in samples, we tried to determine the optimal concentration of normal IgG that inhibits non-specific binding of horse radish peroxidase labeled IgG to staphylococci, and to establish the optimal condition of reaction between antigen-antibody complex and staphylococci. Non-specific binding of horse radish peroxidase labeled specific IgG to staphylococci was almost blocked when the enzyme labeled IgG was 500-fold diluted with phosphate buffered saline containing 2mg/ml of normal IgG. When staphylococci coated with antibody to Salmonella O antigen were mixed with antigen-antibody complex and then incubated for 1 hour at room temperature, the minimal detectable concentration of Salmonella O antigen was 1ng/ml. The sensitivity of enzyme immunoassay was 100-fold greater than a conventional coagglutination method. This enzyme immunoassay could be expected as an improved method for detection of other infectious agents.

• International Journal of Naval Architecture and Ocean Engineering
• /
• 제8권6호
• /
• pp.530-536
• /
• 2016

The number of fabrication shop for spherical type LNG tank is proportional to that of the tank radius to be constructed. Due to limitation of facility investment including building sites, it is practically difficult to fabricate various size tanks with perfectly spherical shape in the yards. The efficient method to be capable of increasing cargo tank volume is to extend vertically the conventional spherical type LNG tank by inserting a cylindrical shell structure. The main purpose of this study is to derive related equations on forces generated on spherical type LNG tank with central cylindrical part under various static loadings in order to establish the simplified analysis method for the initial estimate. In this study, equations on circumferential and meridional force have been derived and verified by relations with the reaction forces per unit length of equator.

• 한국경영과학회지
• /
• 제6권1호
• /
• pp.33-55
• /
• 1981

The so-called “Quick Game”-models resemble a central element within the Compound Gaming approach of military systems analysis proposed by Huber ［1, 2］. This paper introduces such an analytical model designed to investigate the battle-effectiveness of alternative army structures. The nature of its results is discussed from numerical experiments covering the (German) Army Structure 4, Loser's Area Coverage Defence Concept, and Afheldt's Area Defence Concept. It turns out that Loser's concept promises to have a comparatively high potential to stabilize the early conventional forward defence, hence being an interesting alternative for further analysis in depth.

• 대한기계학회논문집A
• /
• 제21권5호
• /
• pp.809-818
• /
• 1997

An efficient non-recursive formulation of dynamic force analysis has been developed for serially connected multibody systems. Although derivation of equations of motion is based on a recursive dynamic formulation with joint relative coordinates, in the proposed formulation, dynamic forces such as joint reaction forces and driving force are computed non-recursively for specified joints. The efficiency of the proposed formulation has been proved by the operational count and the CPU time measure, comparing with that of the conventional recursive Newton-Euler formulation. A simulation of 7-DOF RRC robot arm has been carried out to validate solutions of reaction forces by comparing with those from a commercial dynamic analysis program DADS.

• 대한기계학회논문집A
• /
• 제26권7호
• /
• pp.1288-1295
• /
• 2002

The column-type sensing element in building and mechanical construction parts was designed as three forces and three moments sensor by attaching strain gages approximately. Compared to conventional multi-component sensor, the designed sensor has high stiffness and low cost. The radius of the column was designed analytically and compared with finite element analysis. The interference errors between components were minimized by using addition and subtraction procedure of signals. The fabricated sensor was tested by using a deadweight force standard machine and a six-component force calibration machine. The calibration results showed that the 6-component forces and moments sensor had interference error less than 7.3 % between $F_x$ and $M_x$ components, and 5.0 % in case of other components.

• Journal of Advanced Marine Engineering and Technology
• /
• 제28권2호
• /
• pp.376-381
• /
• 2004

A rig was constructed to test the performance characteristics and compare the hydraulic forces exerted on a centrifugal type artificial heart impeller. A conventional shaft. seal and bearing system. while driven by a small electric motor. supported the impeller which was separated from the pump casing by a six degree of freedom force transducer (JR3 Ine). Radial (x. y) and axial (z) hydraulic forces were recorded and compared. At physiological operating conditions. the results indicate that the double entry/exit centrifugal pump encounters a smaller radial force and significantly reduced axial thrust. These experimental results are valuable in the design of a magnetic bearing system to suspend the impeller of a centrifugal artificial heart pump. This experimental technique may also be applied to evaluate the required capacity and predict the lifetime of contact bearings in marine pumps.

• 한국철도학회:학술대회논문집
• /
• 한국철도학회 2006년도 추계학술대회 논문집
• /
• pp.138-143
• /
• 2006

Oscillatory wheel load fluctuation of considerable amplitude is almost always observed on railway vehicle running at high speed. From the acceleration measured on the axle-box, the frequency of this fluctuation is estimated to be approximately within 70 Hz. By the conventional measuring method, continuous outputs of wheel load can not be obtained, so it is difficult to investigate such a high frequency phenomenon exactly. We have developed a new method of measuring the forces and derailment coefficient continuously, using two pairs of strain gauge bridges whose output phases are shifted by 90 degree, and summing up the outputs with a weighting function. This method is available for measuring the forces between wheel and rail up to high frequency. In this paper, continuous method of measuring forces between wheel and rail and derailment coefficient.

• Journal of Mechanical Science and Technology
• /
• 제20권10호
• /
• pp.1548-1556
• /
• 2006

Appropriate contact force is required for the pantograph on the high speed train to collect current from the catenery system without separation. However, at high speed, large aerodynamic lifting force is generated by the contact plate and the body of pantograph, which may cause wear of the contact wire. In this study, to confirm the interface performance of the pantograph on Korea High Speed Train, a method to measure the contact force of the pantograph was proposed and the related measuring system was developed. The forces acting on the pantograph were clarified and a practical procedure to estimate the forces was proposed. A special device was invented and applied to measure the aerodynamic lifting force. Measured contact forces were displayed by the developed system and evaluated based on the criteria.

• 대한기계학회논문집A
• /
• 제23권6호
• /
• pp.979-987
• /
• 1999

In a high precision vertical machining center, the estimation of cutting forces is important for many reasons such as prediction of chatter vibration, surface roughness and so on, and cutting forces are difficult to predict because they are very complex and time variant. In order to predict the cutting forces of end-milling process for various cutting conditions, a mathematical model is important and this model is based on chip load, cutting geometry, and the relationship between cutting forces and chip loads. Specific cutting force coefficients of the model have been obtained as interpolation function types by averaging farces of cutting tests. In this paper, the coefficients are obtained by neural network and the results of the conventional method and those of the proposed method are compared. The results show that the neural network method gives more correct values than the function type and that in teaming stage as the omitted numbers of experimental data increases the average errors increase.

• Journal of Mechanical Science and Technology
• /
• 제16권6호
• /
• pp.810-818
• /
• 2002

When the contribution of lightweight components to the total energy of a system is small, tole inertia effects are sometimes ignored by replacing them to massless links. For example, a revolute-spherical massless link generates two kinematic constraint equations between adjacent bodies and allows four relative degrees of freedom. In this paper, to implement a massless link systematically in a computer program using the velocity transformation technique, the velocity transformation matrix of massless links is derived and numerically implemented. The velocity transformation matrix for a revolute-spherical massless link and a revolute-universal massless link are appeared as a 6$\times$4 matrix and a 6$\times$3 matrix, respectively. A massless link model in a suspension composite joint transmitting external forces is also developed and the numerical efficiency of the proposed model is compared to a conventional multibody model. For a massless link transmitting external forces, forces acting on links are resolved and transmitted to the attached points with a quasi-static assumption. Numerical examples are presented to verify the formulation.