• Asian Pacific Journal of Cancer Prevention
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• 제15권11호
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• pp.4601-4607
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• 2014

Red rice contains pharmacological substances including phenolics, oryzanol, tocotrienol and tocopherol. Recently, red rice extract has been employed as a source of antioxidants for inhibition of tumor growth. This study was carried out to evaluate the anti-invasion effects of red rice extract fractions on cancer cells. It was found that at $100{\mu}g/ml$ of crude ethanolic extract (CEE), hexane fraction (Hex) and dichloromethane fraction (DCM) could reduce HT1080 and MDA-MB-231 cancer cell invasion. Hex and DCM revealed higher potency levels than CEE, whereas an ethyl acetate fraction (EtOAc) had no effect. Gelatin zymography revealed that Hex decreased the secretion and activity of matrix metalloproteinase-2 and -9 (MMP-2 and-9). In contrast, the DCM fraction exhibited slightly effect on MMPs secretion and had no effect on MMPs activity. Collagenase activity was significantly inhibited by the Hex and DCM fractions. High amounts of ${\gamma}$-oryzanol and ${\gamma}$-tocotrienol were found in the Hex and DCM fractions and demonstrated an anti-invasion property. On the other hand, proanthocyanidin was detected only in the CEE fraction and reduced MDA-MB-231 cells invasion property. These observations suggest that proanthocyanidin, ${\gamma}$-oryzanol and ${\gamma}$-tocotrienol in the red rice fractions might be responsible for the anti invasion activity. The red rice extract may have a potential to serve as a food-derived chemotherapeutic agent for cancer patients.

• International Journal of Oral Biology
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• 제39권3호
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• pp.159-167
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• 2014

Curcumin is a widely used flavoring agent in food, and it has been reported to inhibit cell growth, to induce apoptosis, and to have antitumor activity in many cancers. Cisplatin is one of the most potent known anticancer agents and shows significant clinical activity against a variety of solid tumors. This study was undertaken to investigate the synergistic apoptotic effects of co-treatment with curcumin and cisplatin on human tongue SCC25 cells. To investigate whether the co-treatment efficiently reduced the viability of the SCC25 cells compared with the two treatments separately, an MTT assay was conducted. The induction and the augmentation of apoptosis were confirmed by DNA electrophoresis, Hoechst staining, and an analysis of DNA hypoploidy. Western blot, MMP and immunofluorescence tests were also performed to evaluate the expression levels and the translocation of apoptosis-related proteins following the co-treatment. In this study, following the co-treatment with curcumin and cisplatin, the SCC25 cells showed several forms of apoptotic manifestation, such as nuclear condensation, DNA fragmentation, reduction of MMP, increased levels of Bax, decreased levels of Bcl-2, and decreased DNA content. In addition, they showed a release of cytochrome c into the cytosol, translocation of AIF and DFF40 (CAD) to the nuclei, and activation of caspase-7, caspase-3, PARP, and DFF45 (ICAD). In contrast, separate treatments of $5{\mu}M$ of curcumin or $4{\mu}g/ml$ of cisplatin, for 24 hours, did not induce apoptosis. Therefore, our data suggest that combination therapy with curcumin and cisplatin could be considered as a novel therapeutic strategy for human oral squamous cell carcinoma.

• 동의생리병리학회지
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• 제18권6호
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• pp.1869-1880
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• 2004

In the oriental medicine, a mixture of herbs has been commonly used as important components to control allergic and inflammatory diseases. In the present study, we prepared a mixture of Dictamni Radicis Cortex(Baiksunpee), Houttuyniae Herba(Uhsungcho), and Aurantii Immaturus Fructus(Jisil) to examine its anti-allergic effects in activated mouse splenic cells and found that Mix-1 is involved in regulating levels of B cell activating factors (CD23 and CD11a), IL-1β, IL-6, IL-10, TNF-α, and 1gE as well as HRF expression. It was observed that Mix-1 did not have cytotoxic effects on mLFC. Mix-1 showed inhibition of CD23 and CD11 alpaha expression in mouse B cells, and also decreased the production of IL-6, TNF-α, and 1gE. Both RT-PCR and ELISA analyses indicated that IL-6 and TNF alpha production were regulated at the gene expression level. In contrast, IL-10 mRNA and protein levels were increased in activated B cells by Mix-1 treatment. We also found that Mix-1 inhibited B cell proliferation and inhibited histamine releasing factor(HRF) expression, suggesting its inhibitory effect on histamine secretion. These data indicated that Mix-1 has an anti-allergic effect in activated macrophages and further suggest the possible application of Mix-1 as a therapeutic agent for the treatment of allergy-related diseases.

• 한국방사선학회논문지
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• 제9권4호
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• pp.213-217
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• 2015

스텐트 삽입술을 시행한 환자에게 ASL 방법 중 pCASL을 이용한 관류영상에서 나타난 인공물을 보고하고 이에 대한 해결방법을 제시하고자 한다. pCASL데이터는 구조적 이미지와 함께 스텐트를 피해 표지 펄스(labeling pulse)의 위치를 변경하여 획득하였다. 데이터는 ASLtbx를 이용하여 처리하였다. pCASL을 이용하여 관류영상을 획득하였을 때 기존의 표지 펄스(표지 간격(labeling gap) 24 mm)의 위치가 스텐트의 위치와 겹쳐져서 우뇌 조직의 신호강도가 비어 있는 것처럼 나타났다. 스텐트를 피해 표지 펄스(표지 간격 15 mm)를 위치시킬 때 높은 신호강도의 영상을 획득할 수 있었으며, 표지 펄스(표지 간격 170 mm)에서는 labeled 혈액이 영상절편에 도달하기 전에 이완이 되어 낮은 신호강도의 영상을 획득 하였다. pCASL은 조영제를 사용하지 않기 때문에 안정적으로 반복측정이 가능하며 양질의 영상 획득을 위해서는 알맞은 영상획득인자와 방법들이 선택되어야 한다.

• Archives of Plastic Surgery
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• 제37권5호
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• pp.667-670
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• 2010

Purpose: The management of urethral defect represents one of the most challenging clinical problems in uroplastic surgery. Especially for defect after Fournier's Gangrene, optimal management is still a hard problem. During extensive urethral reconstruction, to overcome the poor vascularity due to periurethral scarred tissue and limitation of the choice of local flap, we report our experience with one-stage reconstruction of urethral defect using a longitudinal tubed flap of scrotal skin. Methods: A 72-year-old man with several years of diabetes mellitus history visited for swelling and pain of scrotal area. After diagnosis of Fournier's Gangrene, radical debridement was performed and 6 cm of urethral defect on border of penile-scrotal ventral area was made. Rectangular scrotal skin flap ($6{\times}2.5\;cm$) based on external spermatic fascia was elevated and tubed longitudinally. After transfer the flap to the defect area, end-to-end anastomosis was performed bilaterally. Results: 4 weeks after the operation, the patient started voiding him-self and urethrography showed good fluence of contrast agent. Long term evaluation reveals stable performance characteristics without any complications. Conclusion: We suggest a one-stage reconstruction of extensive urethral defect using a longitudinal tubed flap of scrotal skin. Advantages of this procedures are simple, one-stage reconstruction with the reliable scrotal skin flap based on external spermatic fascial vasculature, and no donor morbidity.

• Restorative Dentistry and Endodontics
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• 제16권2호
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• pp.99-117
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• 1991

The purpose of this study is to evaluate the effects of dentin bonding agents on the fibroblasts cultivated from human pulp tissue. The fibroblasts were cultured in DMEM/10%FBS medium. Whatman filter paper discs (6mm diameter) soaked with $2{\mu}l$ of dentin bonding agents were placed on a millipore filter (pore size $0.22{\mu}m$) contained in a 50mm Petri dish, and then, exposed for 10 minutes, 30 minutes, 1 hour, 6 hours, 12 hours, 24 hours, 4 days and 7 days in $37.^{\circ}C$, 5% $CO_2$ incubator. The results of the experiments were analyzed by counting the cells and measuring the protein contents at 1 day, 4 days and 7 days. The results of this study were as follows: l. CLEARFIL NEW BOND, LITE-FIL BOND, GLUMA 3 Primer and GLUMA 4 Sealer showed cytotoxicity compared to the control group in the cell counts and the protein contents. 2. GLUMA 4 Sealer showed the least cytotoxicity among the three dentin bonding agents. 3. The results of the cell count were simialr to the results of protein content measurement. 4. LITE-FIL BOND exhibited marked cytotoxicity during 1 day, but, the cytotoxicity was slightly reduced after 4 and 7 days. 5. In GLUMA 3 Primer group, it was not possible to count the cell numbers and measure the protein contents, but the degeneration of cells was observed under the inverted phase-contrast microscope.

• Parasites, Hosts and Diseases
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• 제44권4호
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• pp.331-341
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• 2006

Actin binding proteins play key roles in cell structure and movement particularly as regulators of the assembly, stability and localization of actin filaments in the cytoplasm. In the present study, a cDNA clone encoding an actin bundling protein named as AhABP was isolated from Acanthamoeba healyi, a causative agent of granulomatous amebic encephalitis. This clone exhibited high similarity with genes of Physarum polycephalum and Dictyostelium discoideum, which encode actin bundling proteins. Domain search analysis revealed the presence of essential conserved regions, i.e., an active actin binding site and 2 putative calcium binding EF-hands. Transfected amoeba cells demonstrated that AhABP is primarily localized in phagocytic cups, peripheral edges, pseudopods, and in cortical cytoplasm where actins are most abundant. Moreover, AhABP after the deletion of essential regions formed ellipsoidal inclusions within transfected cells. High-speed co-sedimentation assays revealed that AhABP directly interacted with actin in the presence of up to $10{\mu}M$ of calcium. Under the electron microscope, thick parallel bundles were formed by full length AhABP, in contrast to the thin actin bundles formed by constructs with deletion sites. In the light of these results, we conclude that AhABP is a novel actin bundling protein that is importantly associated with actin filaments in the cytoplasm.

• 대한방사선기술학회지:방사선기술과학
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• 제41권2호
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• pp.109-113
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• 2018

Despite the continuous development of software, it is continuously pursued to enlarge the examination area of FOV in order to reduce the factors of inconsistency in images that appear in continuous examination during wide area examination using contrast agent such as whole body angiography. In this study, we investigated the optimal FOV by comparing the SNR values according to the changes of FOV. The change of the FOV was gradually changed to $270{\times}200$, $300{\times}223$, $330{\times}244$, $360{\times}266$ and $380{\times}281$. SE images at TR 450 msec and TE 10 msec, FSE images at TR 2,000 msec, TE 80 msec, and GE images were scanned at TR 117 msec, and TE 16 msec. SNR values were calculated from the mean values of signal intensities of five phantom images and the signal intensity values of four background standard deviations. As a result of the study, the signal intensity and the SNR value according to the change of the FOV value gradually increased as the FOV was increased, but it was found that the SNR value decreased at a constant size. In conclusion, the results are different from previous studies that the SNR increases as the FOV increases. The cause of these results could not be confirmed. However methods that can be imaged and included within the effective FOV should be considered.

• Journal of Microbiology and Biotechnology
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• 제19권10호
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• pp.1238-1243
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• 2009

The susceptibilities of major enterohemorrhagic Escherichia coli (EHEC) strains to antimicrobial agents and the cytotoxicity of these agents were examined using a total of 38 strains of E. coli O26, O111, and O157, which are the major serogroups of EHEC. Among the 38 strains, 35, 36, and 36 were susceptible to amikacin, imipenem, and norfloxacin, respectively. These antimicrobial agents were further examined to determine their cytotoxicity on Vero cells as well as their effect on the release of Shiga toxins along with trimethoprim/sulfamethoxazole. Each of the E. coli O26, O111, and O157 strains containing both the stx1 and stx2 genes were grown in the absence or presence of these agents at 1/4 minimal inhibitory concentration for 6 h, 12 h, and 18 h. At the concentrations used in this study, none of the agents significantly altered cell count compared with the control group. The level of cytotoxicity in the imipenem group was lower at 12 hand 18 h than their respective controls. In contrast, the level of cytotoxicity in cultures treated with trimethoprim/sulfamethoxazole, norfloxacin, and amikacin was increased. The strains were also examined for the release of Shiga toxins 1 and 2 following treatment with the agents, which were measured by the reversed passive latex agglutination (RPLA) method. The RPLA assay showed a suppression of release of Shiga toxin 2 in the strain cultures containing imipenem. These results indicate that imipenem may be a safe and effective agent for inhibition of these bacteria, which has clinical implications for the treatment of EHEC infections.

• 한국콘텐츠학회논문지
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• 제17권7호
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• pp.215-221
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• 2017

본 연구는 two-point Dixon기법을 이용한 간의 지방정량화 검사 시 가돌리늄 조영제인 Gd-EOB-DTPA가 지방정량화에 어떠한 영향을 미치는지 알아보고자 하였다. 연구방법은 2016년 4월부터 동년 9월까지 two-point Dixon 기법을 이용해 간의 지방정량화 검사를 시행한 60명을, 두 그룹(정상의 간기증자 30명, 비정상의 간 지방증 환자 30명)으로 분류한 후 조영제 주입에 따른 지방분율의 변화를 비교 평가하였다. 연구 결과, 두 그룹 모두 조영제 주입 전 보다 후의 지방분율이 크게 감소(간 기증자 -33.8%, 간 지방증 환자 -47.2%)하는 것으로 나타나 Gd-EOB-DPTA가 지방정량화에 영향을 미친다는 것을 알 수 있었다. 결론적으로 two-point Dixon기법을 이용해 간 지방정량화 검사를 시행할 경우, Gd-EOB-DTPA 주입 전에 영상을 획득해야 지방분율의 변화를 최소화 할 수 있어 정확한 진단을 할 수 있다.