Halberstadt, Craig R.;Palsson, Bernhanrd O.;Midgley, A.Rees;Curl, Rane L.
Biotechnology and Bioprocess Engineering:BBE
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제7권3호
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pp.163-170
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2002
This report describes the use of a transtubular bioreactor to study the relative effects of diffusion versus perfusion of medium on antibody production by a hybridoma cell line. The study was performed with a high-density cell culture maintained in a serum-free, low-protein medium for 77 days. It was determined that the reactor possessed a macro-mixing pattern residence time distribution similar to a continuous stirred tank reactor (CSTR), However, due to the arrangement of the medium lines in the reactor, the flow patterns for nutrient distribution consist of largely independent medium path lengths ranging from short to long. When operated with cyclic, reversing, transtubular medium flow, some regions of the reactor (with short residence times) are more accessible to medium than others (with long residence times). From this standpoint, the reactor can be divided into three regions: a captive volume, which consists of medium primarily delivered via diffusion; a lapped volume, which provides nutrients through unilateral convection; and a swept volume, which operates through bilateral convection. The relative sizes of these three volumes were modified experimentally by changing the period over which the direction of medium flow was reversed from 15 min (larger captive volume) to 9 h (larger swept volume). The results suggest that antibody concentration increases as the size of the diffusion-limited (captive) volume is increased to a maximum at around 30 min with a sharp decrease thereafter. As reflected by changes in measured consumption of glucose and production of lactate, no significant difference in cellular metabolism occurred as the reactor was moved between these different states. These results indicate that the mode of operation of the transtubular bioreactor may influence antibody productivity under serum-free, low-protein conditions with minimal effects on cellular metabolism.
Clothes show not just the different social status of people, but the ideology and value of former society through pattern, colour, materials, shapes, etc. The purpose of this article is to fill the academic blank of this part by researching the pattern of fabric design in Jing Pai(Beiing style) and Hai Pai(Shanghai style) cheong-sam during the period of the Republic of China. The contrastive analysis of regional pattern between Jing Pai and Hai Pai cheong-sam expect to provide the theoretical basis for the former fashion designers and scholars. There are three approaches in the article: Data collection method, comparison method and Combining theory with practice method as film. Regarding components of pattern, both Jing Pai and Hai Pai cheong-sam have mostly single or composite pattern like plants. Further the most of Jing Pai cheong-sam pattern is traditional flower pattern. But Haipai cheong-sam patterns have some western flower pattern. Beside that, it have some geometry pattern. Regarding arrangement of the pattern, both cheong-sams have scattered dot style, the border style, and pictures style. But continuous type of Jing Pai cheong-sam is less while Hai Pai cheong-sam is the most. Comparing Jing Pai cheong-sam color of patterns in "Moment in Peking" is unadorned and types are simple as chinese traditional clothes; However, "In the Mood for Love" introduces us various material colors, new types of patterns and extraneous characteristic geometry patterns of Hai Pai cheong-sam. Generally speaking, the main characteristic of Jing Pai cheong-sam is traditional and conservatism. It keeps Chinese traditional pattern and culture to the most extent. However, Hai Pai cheong-sam are confluent and open with absorbed external culture and techniques which are endowed new artistic color.
The purpose of this study was to classify types of family visitors' behaviors more concretely, which could be useful in planning of exhibition spaces in a science museum, with given priority to their behaviors according to interaction between them, and to understand characteristics of the behaviors. With this view, the scope of this study was restricted to subjects, who were family visitors of Gwacheon National Science Museum, consisting of parents and their one elementary school child per family unit. For understanding their behaviors, itinerary tracking and observation method of behavior were conducted. Moreover, for understanding substantial types and characteristics of family visitors' behaviors, multivariate analysis was applied. Accordingly, the results of the study are as follows. First, as a result of factor analysis, two main factors were derived. In other words, they were 'Durability of viewing' as the first and 'Interactivity between family members' as the second, which were shown valid in classification of the types of their behaviors. Second, according to the factors derived, the types of their behaviors were classified into in-depth cooperative viewing, selective independent viewing, interest concentration child-led viewing and consideration parent-led viewing. They were shown valid in topological localization and classification, as a result of analysis of the main factors. Third, in view of common characteristics of the types, it is judged that active orientation prior to regular viewing could encourage visitors coming to the museum for the first time to feel more friendly to unfamiliar exhibition environments, during the initial seek time, and to reduce visual supersaturation. Fourth, it is judged that distributed arrangement of exhibits, which result in continuous standby, could avoid confused visitor circulation by the estimated time required for viewing.
Indexes has not devcloped as an independent branch in library science from the beginning, but it has gradually evolved in a clo~eas sociation with catalog and under the direct influence of the development of publishing pro cesses and of the rapid social changes. Historically, index in the West can be traced back to eariler concordance. On the other hand, index in the Bast does not show a continuous development. It started with book catnlog, but other types of indexing were later 'adopted from the West. Indexing in the West and in the East can be summarized as follows: 1) In the West, Taylor considers Gesner's Pandectae was the first index but the Concordance of the Bible in 1247 was the first true index. Indexing method was first established later in 1545 in Gesner's Partitiones which appeared in three volumes. Classified index appeared after Partitions, but alphabetically ordered index was not developed until th eseventeenth century. The pxiodical index of La France S~auante in 1683 proved -its value, and Poole's An Alphabetical Index in the nineteenth century became the turning point in the development of indexing. After Poole's Index appeared periodical index and book catalog gradually began to be treated separately, and subject index and cross reference were incorporated into indexing. Also dictionary arrangement of the indexed items was adopted in the second half of the nincteenth, century after Charles A. Cutter developed his theory of rules for dictionary catalog and systematic studies of indexing were carried out by many scholars. In the twentieth century, index was mainly developed in the United States of America, especially by Wilson publishing Company. The general trend is to move away from the gcncral index to subject index. Also the ncwspapcr indcx such as The Times I~zdcx is 21 landmark in the history cf indcxing. 2) In China, thcs arc somc cvidcnccs that $Bizgluh(&), $ was the first indcx, but unforlunatcly the book itsclf has not been found as yet.
This study has been analyzed to write an ideal training evaluation table after analyzing validity of content organization and assigning score of evaluating elements of clinical practice of Dept. of dental technology. 220 junior students who are studying dental technology in D university in Daegu were questioned to analyze the relationship of content organization and score of clinical practice evaluation table. The corrected material has been inspected by SAS V8 for Windows. The results are as following. 1. The validity of content organization of clinical practice evaluation table has a result which can be reliable over than 70% but the validity of score shows low reliance. c 2. The validity of content organization of detail elements of clinical practice evaluation shows that overuse of material and attentiveness(3.78) and responsibility (3.60) are high response but in score organization, the validity shows that safety measure(2.36) when controling machines, arrangement(2.98) after use and ability (2.98) of solving problems are very low. 3. In the elements without interpersonal relation, it shows that those are related to each other. its grading is p < 0.01 so it appears to be meaningful. 4. In result of analyzing the effect to 'studying amount' mark in clinical practice evaluation mark, it appears the element of attitude for job to affect it. And in result of analyzing the effect to 'training attitude' mark, it appears the element of 'controling machines' to affect it. For these results, to be a better evaluation tool, it should be modified by continuous research with arranging evaluation elements to be marked differently in between elements.
STATEMENT OF PROBLEM: Loss of the marginal bone to the first thread have been accepted but continuous effort have been made to reduce this bone loss by varying implant design and surface texture. PURPOSE: This animal study has examined the histomorphometric variations between implants with micro-thread, micro-grooved and turned surfaced neck designs. MATERIAL AND METHODS: Four mongrel dogs have been used the premolars removed and left to heal for three months. One of each implant systems with turned neck, micro-thread and micro-grooved were placed according to the manufacturers’protocol and left submerged for 8 and 12 weeks. These were then harvested for histological examination. RESULTS: The histologically all samples were successfully ossointegrated and active bone remodelling adjacent to implants. With the micro-grooved implants 0.40 mm and 0.26 mm of the marginal bone level changes were observed at 8 and 12 weeks respectively. The micro-threaded implants had changes of 0.79 mm and 0.56 mm at 8 and 12 weeks respectably. The turned neck designed implants had marginal bone level changes of 1.61 mm and 1.63 mm in 8 and 12 weeks specimens. A complex soft tissue arrangement could be observed against micro-threaded and micro-grooved implant surfaces. CONCLUSION: Within the limitations of this study, it could be concluded that implants with micro-grooved had the least and the turned neck designed implants had the most changes in the marginal bone level. The textured implant surfaces affect soft tissue responses.
Urticating spicules and poison -secreting cells of the last instar larva in Euproctis flava BREMER was studied histologically. Three kinds of cells in the epidermis of tubercles on the lst to 8th abodominal segments are classified according to the arrangement of their nuclei : smallepidermal cells, large gland cells, and elongated trichogen cells. As a result of Mallory's triple straining , the epicuticle , the papila-like structure apart form the tubules inside which are gathered at the base and connected with a middle layer cell through a canal in the cuticle, and the peripheral of the urticating spcicule are yellow. However, the inside of the spicule , the tubules within the papilla-like structure, the canal in the cuticle , nuclei in the pidermal cells and the thin exocuticle are red although the thich endocuticle is blue. Particularly , the large nuclei in the middle layer cells are bright red, the cytoplasms of which are little and stained red, too, and the inside of the spicules apt to be stained red when they are broken. The contents therefore seem to be continuous between the spicules and the large cells. Presumably , the large cell at the middle layer is not te tormogen cell which Tsutsumi (1958) has described , but the gland cell which secretes the poison-substance into spicules as Pawlowsky and Stein 91927) and Tonkes (1933) pointed out. Whether the pisonous substance is secreted from the gland cell into the cytoplasmic processes of the trichogen cells which stick large middle layer cells during the formation of the new spicule as Tsutsumi (1958) has observed, or the gland cell makes a new connection with the spicule after the spicule is formed is not clear.
Chang, Won-Il;Shim, Jae-Bum;Hong, Sang-Bum;Lee, Jai H.
Journal of Korean Society for Atmospheric Environment
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제23권E1호
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pp.16-28
/
2007
An improved method was developed to determine gas-phase hydrogen peroxide($H_2O_2$) and organic hydro-peroxides (ROOH) in real-time, The analytical system for $H_2O_2$ is based on formation of hydroxybenzoic acid (OHBA), a strong fluorescent compound. OHBA is formed by a sequence of reactions, photoreduction of Fe(III)-EDTA to Fe(II)-EDTA, the Fenton reaction of Fe(II)-EDTA with $H_2O_2$, and hydroxylation of benzoic acid. By use of this analytical method rather than a previous similar method, Fenton reaction time was reduced from 2 min. to 30s. Air samples were collected by a surfaceless inlet to prevent inlet line losses. With a special arrangement of the sampling apparatus, sample delivery time was drastically reduced from ${\sim}5\;min\;to\;{\sim}20\;s$. The automated system was found to be sensitive, capable of continuous monitoring, and affordable to operate. A comparison of this method with a well-established one showed an excellent linear correlation, validating applicability of this technique to $H_2O_2$ determination. The system was applied to field measurements conducted during summertime of 2004 in Gwangju, South Korea. $H_2O_2$ was found to be a predominant species of peroxides. The diurnal variation of $H_2O_2$ displayed the maximum in early afternoon and the broad minimum throughout night. $H_2O_2$ was correlated positively with ozone, photochemical age, and temperature, however, negatively with $NO_x$ and relative humidity.
Local heat/mass transfer and friction loss in a square duct roughened with various types of continuous and discrete rib turbulators are investigated. The combined effects of the gap flows of the discrete ribs and the secondary flows are examined for the purpose of the reduction of thermally weak regions and the promotion of the uniformity of heat/mass transfer distributions as well as the ;augmentation of average heat/mass transfer. The rib-to-rib pitch to the rib height ratio (p/e) of 8 and the rib angles of 90° and 60° are selected with e/D$\_$h/=0.08. The vortical structure of the secondary flows induced by the parallel angled arrays are quite distinct from that induced by the cross angled arrays. This distinction influences on heat/mass transfer and friction loss in all the tested cases. The gap flows of the discrete ribs reduce the strength of the secondary flows but promote local turbulence and flow mixing. Consequently, the angled discrete ribs with the small gaps provide a more uniform heat/mass transfer distribution sustaining high average heat/mass transfer.
In most direct contact liquid-liquid heat exchangers, oil or hydrocarbon with a density less than water is normally used as dispersed working fluid. The main difficulty that arises with this arrangement lies in the control of the interface at the top of the column. When it is connected with a solar collector which uses water as its working fluid, the main difficulties arise from the fact that the water can be frozen during winter time. In order to solve these problems and to demonstrate the technical feasibility of a direct contact liquid-liquid heat exchanger, liquids heavier than water with low freezing temperature has been utilized as dispersed phase liquids in a small laboratory scale model made of pyrex glass. In the present investigation, dimethyl phthalate(C/sub 6/H/sub 4/)COOCH/sub 3/)/sub 2/) and diethyl phthalate (C/sub 6/H/sub 4/(CO/sub 2/C/sub 2/H/sub 5/)/sub 2/) are utilized as heavy dispersed phase working fluids. The results of the present investigation the technical in the utilization of heavier dispersed working liquid in the spray-column liquid-liquid heat exchanger for a solar system. The overall average temperature difference along the column is found to be almost half of the initial temperature difference between the dispersed and the continuous phase. Despite the fact that the two phthalates tested in the experiment differ significantly in some of their physical properties, the volumetric heat transfer coefficients in terms of dispersed fluid superficial velocities were found to be similar for both phthalates tested.
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