• Title/Summary/Keyword: Content Structure

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Effect of Residual Lithium Ions on the Structure and Cytotoxicity of Silk Fibroin Film

  • Yang, Yesol;Kwak, Hyo Won;Lee, Ki Hoon
    • International Journal of Industrial Entomology and Biomaterials
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    • v.27 no.2
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    • pp.265-270
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    • 2013
  • Dialysis is the rate-limiting step in the preparation of aqueous silk fibroin (SF) solution. However, the traditional practice of dialyzing SF solution for at least 48 h to remove LiBr is not based on empirical evidence. The aim of the present study was to systematically measure LiBr content in SF solutions dialyzed for varying lengths of time and assess the potential toxicity of residual lithium ions in cells. Complete removal of lithium ions was not achieved even after 72 h of dialysis, with a residual lithium ion content in the solution of 22.85 mg/l. SF films prepared from solutions dialyzed for 8 and 24 h had predominantly random coil or b-sheet structures, respectively. The residual lithium had little cytotoxicity in NIH3T3 fibroblast cells, but viability was compromised in cells grown on SF film prepared from solution dialyzed for 24 h.

Characteristics of Water-soluble Polysaccharide, Showing Inhibiting Activity on ${\alpha}-Glucosidase$, in Cordyceps militaris

  • Chung, Ha-Yull;Yoo, Mi-Kyong;Kawagishi, Hirokazu
    • Food Science and Biotechnology
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    • v.18 no.3
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    • pp.667-671
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    • 2009
  • Extract of water-soluble polysaccharide (CFWx), showing inhibiting activity on ${\alpha}-glucosidase$, was prepared from the fruiting bodies of Cordyceps militaris by hot-water extraction, and ethanol precipitation. Chemical characteristics of CFWx were as follows: carbohydrate content 30% including 16% of uronic acid; 51% protein content; monosaccharide composition, Man:Glu:Gal (30:43:27); molecular weight $3-5{\times}10^4$. CFWx was further purified by ion-exchange, gel-permeation, and affinity chromatography and $CFWx-AH-{\alpha}$ fraction was isolated. Fundamental structure of $CFWx-AH-{\alpha}$ was deduced as ${\alpha}-(1{\to}4$)-D-glucan with ${\alpha}-(1{\to}3$)- and/or ${\alpha}-(1{\to}6$)-D-glycosidic side chains based on methylation analysis.

Strain-Stress and Fractural Structure Measurement of EVA, EEA and EBA/Carbon Black Composites (EVA, EEA, EBA와 카본블랙 복합재료의 인장강도 및 파단구조 측정)

  • Yang, J.S.;Lee, K.Y.;Choi, Y.S.;Park, D.H.
    • Proceedings of the KIEE Conference
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    • 2005.07c
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    • pp.1887-1889
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    • 2005
  • To measure the mechanical and structural properties of semiconducting materials in power cable, we have investigated the stress-strain and fractural structure of semiconducting materials showed by changing the content of carbon black. Those were made as sheets after pressing for 20 minutes at $180[^{\circ}C]$ with a pressure of $200[kg/cm^2]$. The contents of conductive carbon black were 20, 30 and 40(wt%), respectively. The stress-strain experiment was measured by TENSOMETER 2000. The SEM experiment was measured by JSM-6400. From above experimental result, Strain was decreased, while stress was increased according to increment of carbon black content. EEA among resins was best the dispersion of carbon back in base resin from SEM measurement.

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A Study On the Mix Design and Quality Control System of High Strength Concrete for the Construct ion of High Rise Complex Structure (초고층 주상복합구조물에 적용한 고강도 콘크리트의 배합설계 및 품질관리 시스템에 관한 연구)

  • Kim, Sun-Gu;Lee, Sang-Soo;Won, Cheol;Park, Sang-Joon;Kim, Dong-Seok
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2001.11a
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    • pp.40-45
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    • 2001
  • The purposes of this study were mix design and quality control of high strength concrete for the construction of high rise complex structure. Desired performances of this high strength concrete were slump flow 50$\pm$10cm, air content 4.5$\pm$1.5% and design strength 400kgf/$cm^2$. Experimental flow was that optimal mix design was selected in the indoor experiment and after that, producing test was done in the batcher plant. Excel lent results of experiment was obtained from binder content 475kg/$m^2$ with replacement ratio 10% of fly ash. The results of field application of high strength concrete was sufficiently satisfied both flowability and compressive strength.

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Interaction of Mastoparan B and Its Ala-Substituted Analogs with Phospholipid Bilayers

  • 박남규;서정길;구희정;김승호;Sannamu Lee;Gohsuke Sugihara;김광호;박장수;강신원
    • Bulletin of the Korean Chemical Society
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    • v.18 no.9
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    • pp.933-938
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    • 1997
  • The interaction of mastoparan B, a tetradecapeptide toxin found in the hornet Vespa basalis, with phospholipid bilayers was investigated. Synthetic mastoparan B and its analogs, obtained by substituting one hydrophilic amino acid (2-Lys, 4-Lys, 5-Ser, 8-Ser, 11-Lys, or 12-Lys) in mastoparan B with Ala, were studied. Mastoparan B and its analogs were synthesized by the solid-phase method. As shown by circular dichroism spectra, mastoparan B and its analogs adopted an unordered structure in buffer solution. All peptides took an α-helical structure, and the α-helical content of its analogs increased in the presence of neutral and acidic liposomes as compared to that of mastoparan B. In the calcein leakage experiment, we observed that mastoparan B interacted more weakly with lipid bilayers in neutral and acidic media than its analogs. Mastoparan B also showed slightly lower antimicrobial activity and hemolytic activity towards human erythrocytes than its analogs. These results indicate that the greater hydrophobicity of the amphiphilic α-helix of mastoparan B by replacement with alamine residues results in the increased biological activity and helical content.

Effects of Polymerization Parameters on Absorption Properties of an Itaconic Acid-based Superabsorbent Hydrogel

  • Kim, Dong Hyun
    • Elastomers and Composites
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    • v.54 no.3
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    • pp.232-240
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    • 2019
  • A superabsorbent hydrogel (SAH) can absorb and retain water weighing more than a hundred times of their dry weight because of their three-dimensional hydrophilic structure. To fabricate an SAH, itaconic acid (IA) and vinyl sulfonic acid (VSA) were subjected to radical polymerization in an aqueous solution, wherein IA and VSA were neutralized, and then, a crosslinker and a thermal initiator were added in sequence. The structure of poly(IA-co-VSA) was characterized using attenuated total reflectance Fourier-transform infrared spectroscopy. We also studied the changes in the absorption properties of the SAH composites according to the polymerization temperature, degree of neutralization, type and content of the initiator used, and type and content of the crosslinker used. Thus, we could determine the effects of some synthetic factors on the absorption properties of the SAH.

An Efficient Dynamic Indexing Model for Various Structure Retrievals of XML Documents (XML 문서의 다양한 구조 검색을 위한 효율적인 동적 색인 모델)

  • 신승호;손충범;강형일;유재수
    • Journal of KIISE:Databases
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    • v.31 no.1
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    • pp.48-60
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    • 2004
  • XML documents consist of elements that are basic units of information. When the structure of XML documents is changed dynamically, we need to update structure information efficiently without changing the information of the index structure for fast retrieval. In this paper, we propose a dynamic indexing model scheme that updates the index structure in real time as the structure of XML documents is changed by insertion and deletion of elements. Our dynamic indexing model consists of a structure information representation method and a dynamic index structure. The structure information representation method supports various types of structure retrievals. Our dynamic index structure processes various structural queries efficiently. We show through various experiments that our method outperforms existing ones in processing various types of queries such as content based queries, structural queries and hybrid queries.

Effect of Oxygen on the Microstructure and Mechanical Properties of Cr-O-N Coatings (Oxygen 함량에 따른 Cr-O-N 코팅막의 미세구조 및 기계적 특성에 관한 연구)

  • Yun, Jun-Seo;Kwon, Se-Hun;Park, In-Wook;Lee, Jeong-Du;Kim, Kwang-Ho
    • Journal of the Korean institute of surface engineering
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    • v.42 no.5
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    • pp.220-226
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    • 2009
  • Cr-O-N coatings having different oxygen contents were deposited on Si wafer and SUS 304 substrates by an arc ion plating technique using Cr target in $Ar/O_2/N_2$ gaseous atmosphere. As increasing oxygen content in the coating, the microstructure of Cr-O-N coating changed from polycrystalline having NaCl structure to amorphous structure. Further increase of oxygen content resulted in phase transformation from amorphous to rhombohedral structure. From the variations of d value and average grain size, it was revealed that the maximum solubility of oxygen in Cr-O-N coating was about 21 at.%. And the maximum micro-hardness of 2751HK was obtained in this composition. The lowest friction coefficient was measured in the coating having 34.8 at.% of oxygen. However, more narrow width of wear track was found in the coating having 30.1 at.% of oxygen.

STUDY OF THE MARINE CLOUD STRUCTURE WITH AQUA AMSR-E

  • Shoom, Mariya Yu.
    • Proceedings of the KSRS Conference
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    • v.2
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    • pp.1007-1010
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    • 2006
  • This study investigates the spatial structure of the total cloud liquid water content Q fields over the Northwest Pacific Ocean during winter monsoon. The distributions of Q have been estimated from the brightness temperatures of the ocean - atmosphere system $T_B(f)$, where f is frequency, measured by AQUA AMSR-E in January -March 2003. Marine strati (St) and stratocumuli (Sc) are typical for winter monsoon season. They were analysed using mainly high-frequency channel at f = 36.5 GHz, vertical polarisation. $T_B$ data were accompanied by the data on near surface wind speed, air temperature and humidity from the nearest meteorological stations. Tow one-dimensional spectra were computed for downwind and crosswind sections of Q fields. The AMSR-E antenna field of view (14-8 km) and the cloud field sizes (100-1000 km) restricted the spatial scales. The results of case study Jan 31 2003 are presented. Scale-invariant spectrum is typical. In the cases of extended St levels a spectral slope equals about -1.7, conforming to classical -5/3 of turbulence theory. For Sc cases the absolute magnitude of spectral slope is rather higher, as a rule. The value is about -2. In the case when cloud streets are presented, a strait line form of spectrum is less reliable with a slope being rather lower (about -1.4).

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Structure and Antibacterial Property of ZnO-B2O3-P2O5 Glasses

  • Bae, Jun-Hyeon;Cha, Jae-Min;Kim, Dae-Sung;Kim, Young-Seok;Ryu, Bong-Ki
    • Journal of the Korean Ceramic Society
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    • v.55 no.2
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    • pp.135-139
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    • 2018
  • The glass structure and antibacterial properties of $(65-x)ZnO-xB_2O_3-35P_2O_5$ glasses were investigated. Zinc borophosphate glasses were prepared using a conventional melt-quenching technique at $1000^{\circ}C$. Glass transition temperature and CTE were studied and the structure of zinc borophosphate glasses was evaluated by FTIR. The $Zn^{2+}$ state increase with increasing ZnO content was investigated by XPS and a single sharp Zn $2P_{3/2}$ peak was confirmed, showing that Zn $2P_{3/2}$ exists as $Zn^{2+}$. In order to to evaluate the antimicrobial activity, Escherichia coli (E. coli) was used following the Japanese Industrial Standard JIS Z 2801; the E. coli death rate was found to increase with increasing $Zn^{2+}$ content of glasses.