• Journal of Korean Neurosurgical Society
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• v.29 no.4
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• pp.471-476
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• 2000

Objective : p16/INK4a, a kind of tumor suppressor genes, encodes a specific inhibitor of the cyclin D-dependent kinases CDK4 and CDK6. This prevents the association of CDK4 with cyclin D1, and subsequently inhibits phosphorylation of retinoblastoma tumor suppressor protein(pRb), thus preventing exit from the G1 phase. According to previous reports, over 50% of glioma tissue and 80% of glioma cell lines have been demonstrated inactivation of p16/INK4a gene. The purpose of this study was to determine whether recombinant adenovirus-p16 virus is a suitable candidate for gene replacement therapy in cases of glioma. Methods : Three human glioma cell lines(U251MG, U87MG and U373MG) that express mutant p16 protein were used. Replication-deficient adenovirus was utilized as an expression vector to transfer exogenous p16 cDNA into the cells ; control cells were infected with the Ad-${\beta}$-gal expressing ${\beta}$-galactosidase. To monitor gene transfer and the expression of exogenous genes, we used Western Blotting analysis. Flow cytometry studies of cellular DNA content were performed to determine the cell cycle phenotype of the glioma cells before and after treatment. Results : We showed here that restoration of p16/INK4a expression in p16 negative U87MG, U251MG and partially deleted U373MG by Ad-CMV-p16 induced growth suppression in vitro. Flow cytometric study revealed that Ad-CMV-p16 infected U87MG cells were arrested during the G0-G1 phase of the cell cycle. Expression of p16 transferred by Ad-CMV-p16 in glioma cells was highly efficient and maintained for more than seven days. Conclusions : Our results suggest that Ad-CMV-p16 gene therapy strategy is potentially useful and warrants further clinical investigation for the treatment of gliomas.

• Animal Bioscience
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• v.34 no.12
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• pp.2023-2033
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• 2021

Objective: The present study was conducted to assess the effect of urease inhibitor (hydroquinone [HQ]) and nitrification inhibitor (dicyandiamide [DCD]) on nitrogen (N) use efficiency of pig slurry for perennial ryegrass regrowth yield and its environmental impacts. Methods: A micro-plot experiment was conducted using pig slurry-urea ¹⁵N treated with HQ and/or DCD and applied at a rate of 200 kg N/ha. The flows of N derived from the pig slurry urea to herbage regrowth and soils as well as soil N mineralization were estimated by tracing pig slurry-urea ¹⁵N, and the N losses via ammonia (NH₃), nitrous oxide (N₂O) emission, and nitrate (NO₃^-) leaching were quantified for a 56 d regrowth of perennial ryegrass (Lolium perenne) sward. Results: Herbage dry matter at the final regrowth at 56 d was significantly higher in the HQ and/or DCD applied plots, with a 24.5% to 42.2% increase in ¹⁵N recovery by herbage compared with the control. Significant increases in soil ¹⁵N recovery were also observed in the plots applied with the inhibitors, accompanied by the increased N content converted to soil inorganic N (NH₄⁺+NO₃^-) (17.3% to 28.8% higher than that of the control). The estimated loss, which was not accounted for in the herbage-soil system, was lower in the plots applied with the inhibitors (25.6% on average) than that of control (38.0%). Positive effects of urease and/or nitrification inhibitors on reducing N losses to the environment were observed at the final regrowth (56 d), at which cumulative NH₃ emission was reduced by 26.8% (on average 3 inhibitor treatments), N₂O emission by 50.2% and NO₃^- leaching by 10.6% compared to those of the control. Conclusion: The proper application of urease and nitrification inhibitors would be an efficient strategy to improve the N use efficiency of pig slurry while mitigating hazardous environmental impacts.

• Cross-Cultural Studies
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• v.50
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• pp.197-224
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• 2018

Korea's Kimjang and Japan's Washoku were listed in the UNESCO's Intangible Cultural Heritage of Humanity in 2013. Kimjang and Washoku not only have the meaning of a long tradition or national representative food. Additionally, UNESCO admitted the value of Kimjang and Washoku that allows members of society to share together and strengthen their solidarity through the use of it. And the identity of each nation is well represented in the Kimjang and Washoku effort. The Kimjang is managed by the Intangible Cultural Properties Department of the Cultural Heritage Administration. Likewise, the Washoku is managed by the Department of Washokusitsu of Japan's Agriculture Ministry. In regards to festivals, they are the most representative of Kimjang's contents in Korea. Kimjang can participate with a consensus of all people and must do it before the coming winter months. In this way, it is very suitable for the presentation of a festival. And there are many festivals related to Washoku in Japan, mainly manifested with local specialties. We have seen that Japan has many kinds of festivals, which encourages it to be called and known as the country of festivals. And Washoku is being fostered as the core content of the 'Cool Japan Strategy' because it has become world-renowned since it was listed as an intangible cultural heritage.

• Korean Journal of Environmental Agriculture
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• v.37 no.3
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• pp.172-178
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• 2018

BACKGROUND: Composted animal manure applied to the arable soil for improving soil quality and enhancing crop productivity causes greenhouse gas emissions such as nitrous oxide ($N_2O$) by processes of nitrification and denitrification. However, little studies have been conducted on determining effect of application ratio of composted animal manure on $N_2O$ emission rate and its annual emission pattern from upland soil in South Korea. Therefore, this study was conducted to determine $N_2O$ emission rate and its annual emission pattern from upland soil supporting for sweet potato. METHODS AND RESULTS: Composted animal manure was applied at the ratio of 0, 10, and 20 Mg/ha to an upland soil supporting for sweet potato (Ipomoea batatas). Nitrous oxide emission was examined during growing season and non-growing season from May 2016 through May 2017. Daily $N_2O$ fluxes showed peaks right after applications of composted animal manure and inorganic nitrogen fertilizer. Precipitation and soil water content affected daily $N_2O$ flux during non-growing season. Especially, $N_2O$ flux was strongly associated with water filled pore space (WFPS). We assumed that the majority of $N_2O$ measured during growing season of sweet potato was produced from nitrification and subsequent denitrification. Annual cumulative $N_2O$ emission rate significantly increased with increasing application ratio of composted animal manure. It increased to 12.0 kg/ha/yr from 8.73 kg/ha/yr at control with 10 Mg/ha of composted animal manure and to 14.0 kg/ha/yr of $N_2O$ emission with 20 Mg/ha of the manure. CONCLUSION: To reduce $N_2O$ emission from arable soil, further research on developing management strategy associated with use of the composted animal manure and soil moisture is needed.

• Journal of Digital Convergence
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• v.17 no.3
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• pp.1-11
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• 2019

This study is aimed to analyze university students' learning experiences in K-MOOC by conducting a survey of university students' perception of K-MOOC, who are the major users of K-MOOC. Also it intended to use them to develop a specific strategy for continued use of K-MOOC. The study was conducted on 155 university students who attending university course and experienced K-MOOC's course within a year. The survey was conducted on online and provided with multiple questions about sustainability and satisfaction. Also survey provided with a subjective items about reason of satisfaction and dissatisfaction with the K-MOOC, and expectation about the future K-MOOC. The analysis found that the main motivation for university students was the convenience of online learning, which was the highest in questions asking why they were satisfied. For reasons of dissatisfaction, the decrease in academic volition and lack of interaction, and the technical problems of K-MOOC system were pointed out. University students learners are expecting improvements in course content and K-MOOC system. Based on these results, this paper suggests instructional strategies to help K-MOOC university students for becoming lifelong learners of K-MOOC.

• The Journal of the Korea Contents Association
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• v.18 no.12
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• pp.1-13
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• 2018

Senior business has been paid attention due to rapid global aging and baby boomers turning 60. However, senior business is not successful as expected because of a lack of understanding of consumers and small size of Korean domestic market. Understanding of senior consumers and overseas markets and providing attractive product and service contents needs to overcome the current challenges. Therefore, this study explores and compares consumers of different generation and different countries. We employ consumption value theory and analyze which consumption values are perceived strongly by each four group(Korean consumers in their 20s, Korean their consumers in 50s, Chinese consumers in their 20s, and Chinese consumers in their 50s). We conducted online survey to compare consumption values among samples of consumers in two countries: Korea and China. There are forty survey instruments in twelve consumption values. The results can be used to develop a strategy for planning, developing, distributing, and promoting product and service contents which consumers, in particular baby boomers, have a strong intention to purchase it.

• The Journal of the Korea Contents Association
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• v.19 no.1
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• pp.163-173
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• 2019

The purpose of this study is to analyze the influence relationship of spectators' evaluation, perceived value, overall satisfaction, and loyalty on the service quality of Goyang International Flower Exhibition. As a result of analysis, first, it turned out that the higher the quality of the fair service, the higher the perceived value, overall satisfaction and loyalty. Especially, fair service quality showed a greater influence on overall satisfaction than on perceived value. Therefore, in order to improve the quality of the fair service, careful planning and operation of event content, accessibility, and convenience are required. Second, perceived value did not have a positive (+) effect on overall satisfaction, but it had a positive (+) effect on loyalty. The reason for this is that the spectators' evaluation of the perceived factors that can induce the interest of the flower product and the increase of the purchase, such as the quality problem of the product, the design problem, the exhibition and display problem, was low. Third, both perceived value and overall satisfaction had a positive (+) effect on loyalty, but the effect of overall satisfaction was greater than that of perceived value. Therefore, a strategy for enhancing the perceived value is required.

• Journal of Soil and Groundwater Environment
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• v.23 no.6
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• pp.90-103
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• 2018

Although fluoride is an essential trace element, ingestion of excessive amount of fluoride could have detrimental effect on human health. Generally, the bioavailability of fluoride in soils was low, but it could be harmful to the environment depending on the soil properties. Therefore, it is necessary to understand the concentration distribution, and fate and transport characteristics of fluoride to establish a resonable management strategy for fluoride pollution. This study was conducted to evaluate nationwide fluoride distribution in soils in Korea, as well as its fate and transport characteristics. The average background concentration was 204.5 (15.3~504.8) mg/kg, which is lower than the values of foreign soils. For the three regions of different land use, the average concentration was 229.6 mg/kg in region 1, 195.7 mg/kg in region 2, and 273.4 mg/kg in region 3. The concentration of fluoride was the highest in soils from Youngnam block within tectonic structure derived from metamorphic rocks. The results of sequential extraction to access F bioavailability showed fluoride in soils mainly existed as a residual form, which suggests the bioavailability of fluoride was relatively low. Soil properties such as soil pH, CEC, and clay content were found to affect F bioavailability of soil.

• 한국노년학
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• v.37 no.3
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• pp.583-600
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• 2017

The purpose of this study is to explore qualitatively what kind of coping strategy the Korean older adults use when they are depressed and why. Participants were users, older than 60, of an elderly welfare center in Seoul, and answers of 34 respondents who experienced depression were included in the analysis. The collected data were analyzed through content analysis. The results of the analysis showed that strategies to cope with depression used by participants were composed of 6 domains and 11 sub-domains: health behavior (medical approach / exercise and diet), family and social contact (social interaction / going out and going on a trip / communication with family), Religious activities (Religious activities), lifelong education (hobbies / educational activities), productive activities (labor), health risk behaviors (drinking and gambling / resignation). The depression coping strategies of the older adults and their characteristics are as follows. First, older adults used diverse problem-focused coping strategies to cope with depression. Second, older adults considered various coping strategies together and used them simultaneously. Third, the facility space for older adults functions as a shelter. Fourth, although there were cases where medical approach was used, the intention to reuse was very low. Through this study, the following suggestions were made to help older adults cope with depression more successfully. First, access to various coping strategies should be promoted. Second, more places where older adults can spend their time comfortably need to be provided. Third, resistance to mental health care should be resolved.

• Journal of the Korean Electrochemical Society
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• v.21 no.4
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• pp.80-87
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• 2018

Safety issues in Li-ion battery system have been prime concerns, as demands for power supply device applicable to wearable device, electrical vehicles and energy storage system have increased. To solve safety problems, promising strategy is to replace organic liquid electrolyte with non-flammable solid electrolyte, leading to the development of all-solid-state battery. However, relative low conductivity and high resistance from rigid solid-solid interface hinder a wide application of solid electrolyte. Composite electrolytes composed of organic and inorganic parts could be alternative solution, which in turn bring about the increase of conductivity and conformal contact at physically rough interfaces. In our study, composite electrolytes were prepared by combining poly(ethylene oxide)(PEO) and $Li_7La_3Zr_2O_{12}$ (LLZO). The crystallinity, morphology and electrochemical performances were investigated with the control of LLZO contents from 0 wt% to 50 wt%. From the results, it is concluded that optimum content and uniform dispersion of LLZO in polymer matrix are significant to improve overall conductivity of composite electrolyte.