• Title/Summary/Keyword: Contamination Test

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Contamination of Chinese Cabbage Soil with Plasmodiophora brassicae (배추 산지 토양의 뿌리혹병균 오염)

  • Soh, Jae-Woo;Han, Kyung-Sook;Lee, Seong-Chan;Lee, Jung-Sup
    • Research in Plant Disease
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    • v.19 no.3
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    • pp.201-207
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    • 2013
  • This research was performed to establish basic technology for Chinese cabbage clubroot chemical control by investigating the soil contamination of Plasmodiophora brassicae in major producing regions of fall Chinese cabbage. PCR primers were developed to detect P. brassicae, a causal agent of Chinese cabbage club-root that generally occurs in Cruciferae family. A primer set, PbbtgF761 and PbbtgR961, specifically amplified a 245 bp fragment from P. brassicae only. At places well known for fall Chinese cabbage, 10 out of 33 in Haenam-gun, 5 out of 13 in Yeongam-gun and Yeonggwang-gun, 1 out of 6 in Gochang-gun, 2 out of 12 in Hongseong-gun, and 5 out of 17 in Dangjin-si resulted positive for P. brassicae contamination. The results show that the soil contamination rate of P. brassicae was 30.3% in Haenam-gun, 38.5% in Yeongam-gun and Yeonggwang-gun, 16.7% in Gochang-gun, 16.7% in Hongseong-gun, and 29.4% in Dangjin-si. The six places where Chinese cabbage clubroot was visible by naked eye were 100% confirmed by the PCR test of the P. brassicae contaminated soil. Thus, simple PCR test may be utilized as an index to decide on chemical control of P. brassicae.

Applying Alcock-Paczynski Test to the Large Scale Structure

  • Li, Xiao-Dong;Park, Changborm;Romero, Jaime Forero
    • The Bulletin of The Korean Astronomical Society
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    • v.38 no.2
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    • pp.58.1-58.1
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    • 2013
  • The main idea of the Alcock-Paczynski (AP) test is that, if we use a wrong distance-redshift relation to infer the shape of a spherical object in the Universe, this object may look non-spherical. To probe the cosmic expansion history through the AP test, the key point is to find something which is known as spherical in the Universe. We propose two possible ways applying the AP test to the large scale structure (LSS): 1) Based on the observed galaxies or quasars, one built up the beta-skeleton tracing the LSS, and investigating the inhomogeneity of the connections; 2) One reconstructs the smoothed density-contrast gradient field based on LSS observations, and investigating the inhomogeneity of the gradient vectors. Compared with some existed methods probing AP effect through 2-point correlation function, galaxy pairs, or voids, our methods have various advantages: 1) The information of both the high and low dense regions of the LSS are taken into account. 2) The redshift space distortion as the main contamination to the AP effect can be easily removed.

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Artificial contaminated test method and leakage current characteristics of EHV insulators (송전용 애자의 인공오손시험 기법과 누설전류 특성)

  • Choi, In-Huyk;Jung, Yoon-Hwan;Choi, Jang-Hyun;Lee, Dong-Il
    • Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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    • 2004.05a
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    • pp.386-391
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    • 2004
  • To find out the reason of accident by being stained of insulators, this paper researched and analyzed the result of electrical characteristic test and field test of EHV insulator through artificial contaminated method. The best way to evaluate contaminated level of insulator surface is getting a contamination data by regional groups continually. But it is needed a lot of time and manpower. To overcome those difficulties, this research introduced ESDD(equivalent salt deposit density) measurement method which is adapted to the international codes of IEC and compared the relation between experimental test results which contaminated insulators by going through stain procedure and electrical measurement results.

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Identification and Antibiotic Susceptibility Test of Bacteria from In Vitro Cultures of Pinellia ternata and Tea Plant (반하 및 차나무의 기내배양시 발생하는 세균의 동정 및 항생제 감수성 검정)

  • Kim, Haeng-Hoon;Cho, Gyu-Taek;Yoon, Mun-Sup;Yoon, Ju-Won;Cho, Eun-Gi
    • Journal of Plant Biotechnology
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    • v.30 no.2
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    • pp.189-194
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    • 2003
  • Contamination of bacterial infection is one of serious problems in in vitro culture system of root crops. From the contaminated tubes over 140 of petiole cultures of Pinellia ternata, a medicinal plant, 4 genera 8species 48 strains of bacteria, including Aeromonas and Pseudomonas, were isolated and identified and another 8 strains were not fully identified. Most of them were motile Gram positive bacteria as in common in early stage of in vitro cultures. Six strains of bacteria, 5 of Gram negative, including Enterobacter, and 1 of Gram positive, were identified from the embryonic axes cultures of tea plant. From the susceptibility test to pre-screened 5 antibiotics, all of the bacteria except for 2 species of Pseudomonas were susceptible to cefotaxime 60∼100mg/L. While 60mg/L erythromycin only was effective to Pseudomonas. Combination of erythromycin 20mg/L and cefotaxime 60mg/L totally suppressed the growth of all bacterial strains tested. Susceptibility test of bacteria from tea embryonic axes cultures showed similar results. Combination of erythromycin 35mg/L and cefotaxime 60mg/L was effective to 15 bacterial strains and partially effective to 1 unidentified.

The Effect on Science-Related Attitudes of High School Students for Newspaper in Education(NIE) (NIE 수업이 고등학생들의 과학과 관련된 태도에 미치는 영향)

  • Joo, Min-Sun;Youn, Seok-Tai;Koh, Yeong-Koo;Kim, Jong-Hee;Oh, Kang-Ho
    • Journal of the Korean Society of Earth Science Education
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    • v.9 no.1
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    • pp.27-38
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    • 2016
  • Through the systematic analysis on articles about the environment in newspapers, the news reports were considered as effective materials, so after being reconstructed, they were used as learning materials in a lesson on environment contamination which is newly introduced in Earth Science I from the 2009 curriculum revision. To examine students' attitude toward science, before and after NIE application to the students, Test of Science Related Attitudes(TOSRA) as a test tool was carried out which was composed of 7 categories - four optional questionnaires for each category, 28 in total were used. The result was as follows: in a pretest, based upon the average scores by factors the factors ranked from the highest to the lowest as follows: the social meaning of science, the acceptance of the scientific attitude, the commonness of a scientist, the attitude to scientific exploration, the pleasure in science class, the vocational interest in science, the concern over science as a hobby, and in posttest, their ranking as follows: the acceptance of the scientific attitude, the pleasure in science class, the commonness of a scientist, the social meaning of science, the attitude to scientific exploration, the vocational interest in science, the concern over science as a hobby. Also they were all statistically significant at a significant level p<0.05. However, the test revealed that there were some negative effects on the social meaning of science and the attitude to scientific exploration and therefore it is judged that in order to overcome those influences, some bright articles about solving the environment contamination should be applied to the lessons and be complemented effectively by experiments and various media: NIE as well as science magazines, data from Internet search and treatises on science.

Relationship between Extraction Methods of Copper in Soil and the Bioaccumulated Copper in Earthworm (Microcosm soil test를 이용한 지렁이 체내 축적 구리 농도와 구리 침출법 간의 상관관계 비교)

  • Choi, Youn-Seok;Kim, Kye-Hoon
    • Korean Journal of Soil Science and Fertilizer
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    • v.40 no.4
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    • pp.298-310
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    • 2007
  • This study compared the correlation between the accumulated copper content in earthworms and the copper concentration rate of soil measured using several methods to extract heavy metals from soil. For the experiment, a microcosm soil test was carried out using copper contaminated soil from the vicinity of copper-roofed buildings and earthworms (Eisenia fetida). Soils from the study area were used to produce 6 treatments; control, 1C (contamination level with the lowest treated copper concentration rate), 2C, 4C, 8C, and 16C (contamination level with the highest treated copper concentration rate). Microcosm soil test using the 6 treatments proved that as the copper content in soil and the experiment time increased, the growth rate of and the accumulated copper concentration rate in earthworms increased as well. The degree of the increase corresponded to the order of the treated copper concentration levels in microcosm soils. Standard method of the ministry of environment and EPA method 3051 were used to obtain the copper concentration in soil and the total copper content in soil, respectively. The correlation coefficient (r) of 0.9875~0.9993 between the copper content extracted by the standard method and the total copper content shows high positive correlation. The correlation coefficient of the copper content in soil extracted by the standard method and the accumulated copper content in earthworms, and the correlation coefficient of the total copper content in soil and the accumulated copper content in earthworms were ranged from 0.9193 to 0.9728 and from 0.9282 to 0.9844, respectively, showing highly significant positive correlation. Due to the high correlation between the copper concentration in soil and the accumulated copper content in earthworms, it is concluded that earthworms are suitable to be used as biological indicator species or for bio-monitoring against copper contamination of soil.

Evaluation of Oil Infiltration Behavior in Porous Media Using Dielectric Response (유전율에 의한 지반 매질내 유류침투거동 분석)

  • Kim Man-Il;Jeong Gyo-Cheol
    • The Journal of Engineering Geology
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    • v.15 no.1
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    • pp.29-39
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    • 2005
  • For detecting a ground contamination survey, soil sampling method have been used a drilling or coring technique in general. However these methods are very difficult to systematically real-time monitoring of variation of contamination degree in field. ]'n this research frequency Domain Reflectometry (FDR) system was suggested and carried out to experimental approaches for determination of oil contamination on surface and underground. Experimental method using FDR method was discussed with feasibility of measurement in the laboratory column test. It is determined to degree of oil contamination due to response of dielectric constant re-lated with volumetric water content(θ/sub w/) and volumetric oil content( θ/sub al/ ) of saturated and unsaturated soil media. And physical properties such as effective porosity and oil residual ratio of saturated soil media were also measured through real-time monitoring works using installed FDR measurement sensors, which are defected characteristics of oil movement in the saturated soil media under the soil column tests. In the results of these experiments, a range of effective porosity was estimated to about 0.35 compared with initial porosity 0.40 of manufactured saturated soil media, which is also calculated to about 87.5% to the ratio of initial porosity to effective porosity. Finally oil residual ratio which is compared with volumetric water content and volumetric oil content was calculated about 62.5%.

Survey of the oil contaminated level and preliminary field bioremediation test in the Mountain Baegun at Uiwang city (의왕시 백운산 주변 유류 오염도 조사 및 현장 복원 기초실험)

  • 김종석;주춘성;김윤관;권은미;정욱진
    • Journal of Soil and Groundwater Environment
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    • v.7 no.2
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    • pp.3-11
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    • 2002
  • The objective of this study was to survey the oil contamination around the Mountain Baegun at Uiwang city to obtain the preliminary data for bioremediation. For measuring the oil concentrations and physical properties from soil, we analyzed BTEX. TPH and pH, organic content, water content, pormeability coefficient, gravity, porosity and used the purge & trap method for analyzing BTEX. Using the Accelerated Solvent Extractor, we pretreated the samples and then analyzed TPH using GC-FID as soon as possible. From the analysis results, maximum concentration of TPH was 24.773mg/kg and BTEX was 101.7mg/kg. The results of TPH at the Mountain Baegun were higher than the enforcement standard of soil contamination(Korea) and the BTEX concentrations were also higher than the advisory standard of soil contamination(Korea). From these results, the Mountain Baegun may requires to remedy the oil-contaminated soil. In addition, we performed the field bioremediation test for five weeks at the Mountain Baegun using the microbial additives that were developed by our laboratory. From the results of the field test, we could find the about 95% of the oil was removed from the contaminated soil in five weeks. So we consider that it is the one of the useful solutions to remedy the oil-polluted site.

Characteristics of Fancy Veneer Plywood Floor using Green Tea Leaves Powder (녹차잎분말을 사용한 마루판의 특성)

  • Kang, Seog-Goo;Lee, Hwa-Hyoung
    • Journal of the Korea Furniture Society
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    • v.21 no.4
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    • pp.284-292
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    • 2010
  • This research was carried out to examine the properties of fancy cherry veneer overlaid on the PF resin bonded Meranti plywood floor, which 2.5% green tea leaf powder was applied in the UV varnishes and the adhesives for scavenging the volatile organic compounds. The results were as follows: 1. The various properties of the treated samples, such as density, moisture content, thickness swelling, bending strength(MOR), adhesion shear strength, surface abrasion, curling, cyclic delamination test with boiling water, boiling property, cold-resistance and heat resistance, acid resistance and alkali resistance, and anti-contamination property showed no significant difference between the properties of the control samples. 2.5% green tea leaf powder treated floor gave a little better results than the control for surface scratch test. 2. In case of QUV and weathering test, no difference between the treated sample and control was found. 3. The floor was discolored by adding 10% green tea leaf powder to UV coating, and the floor was also discolored to light green during by the soaking test. The color of floor was not changed up to 5% addition level.

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A Development of Landfill Liner by Utilizing Waste Lime (폐석회를 이용한 매립지 차수재 개발)

  • 김준섭;이승학;박준범
    • Proceedings of the Korean Geotechical Society Conference
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    • 1999.03a
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    • pp.521-528
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    • 1999
  • As the size of our industry and population inclose, the byproducts such as municipal solid wastes, industrial wastes are in the increasing phase. The treatment of such things is rising as a social problem. Today, the final disposal of these wastes depends mostly on the landfill, and the sanitary landfill is required and designed for preventing soil and groundwater contamination. Clays have been used for a liner material of a sanitary landfill, however, the high quality clay is hard to come by and quite expensive as a lining material in our country. Using the waste lime produced abundantly every year from chemical processes was studied here, made from the proper mixing of the bentonite and the waste lime meets the regulations from the USEPA. The soil property index tests (sieve analysis, specific gravity test Atterberg limit test) were performed, and at last to confirm the sorption characteristics of the bentonite and the waste lime the sorption isotherm equilibrium test and the sorption isotherm were performed with Toluene and Ethylbenzene which are the main components of the leachate from the landfill.

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