Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Contamination of Chinese Cabbage Soil with Plasmodiophora brassicae

배추 산지 토양의 뿌리혹병균 오염

  • Soh, Jae-Woo (Department of Horticultural Environment, National Institute of Horticultural and Herbal Science) ;
  • Han, Kyung-Sook (Department of Horticultural Environment, National Institute of Horticultural and Herbal Science) ;
  • Lee, Seong-Chan (Department of Horticultural Environment, National Institute of Horticultural and Herbal Science) ;
  • Lee, Jung-Sup (Department of Horticultural Environment, National Institute of Horticultural and Herbal Science)
  • 소재우 (국립원예특작과학원 원예특작환경과) ;
  • 한경숙 (국립원예특작과학원 원예특작환경과) ;
  • 이성찬 (국립원예특작과학원 원예특작환경과) ;
  • 이중섭 (국립원예특작과학원 원예특작환경과)
  • Received : 2013.04.17
  • Accepted : 2013.07.26
  • Published : 2013.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

This research was performed to establish basic technology for Chinese cabbage clubroot chemical control by investigating the soil contamination of Plasmodiophora brassicae in major producing regions of fall Chinese cabbage. PCR primers were developed to detect P. brassicae, a causal agent of Chinese cabbage club-root that generally occurs in Cruciferae family. A primer set, PbbtgF761 and PbbtgR961, specifically amplified a 245 bp fragment from P. brassicae only. At places well known for fall Chinese cabbage, 10 out of 33 in Haenam-gun, 5 out of 13 in Yeongam-gun and Yeonggwang-gun, 1 out of 6 in Gochang-gun, 2 out of 12 in Hongseong-gun, and 5 out of 17 in Dangjin-si resulted positive for P. brassicae contamination. The results show that the soil contamination rate of P. brassicae was 30.3% in Haenam-gun, 38.5% in Yeongam-gun and Yeonggwang-gun, 16.7% in Gochang-gun, 16.7% in Hongseong-gun, and 29.4% in Dangjin-si. The six places where Chinese cabbage clubroot was visible by naked eye were 100% confirmed by the PCR test of the P. brassicae contaminated soil. Thus, simple PCR test may be utilized as an index to decide on chemical control of P. brassicae.

본 연구는 가을 배추 산지로 알려진 지역의 토양내 P. brassicae 의 오염 조사를 통하여 배추 뿌리혹병 방제의 기초 기술을 확립하고자 수행하였다. 배추과 작물에 뿌리혹을 유발하는 P. brassicae를 검출하기 위한 PCR primer를 개발하였다. PbbtgF761과 PbbtgR961의 primer 세트는 P. brassicae에 특이적인 245 bp 크기의 밴드를 증폭하였다. 가을 배추 재배지로 알려진 해남군은 33곳 중 10곳, 영암군 및 영광군은 13곳 중 5곳, 고창군은 6곳 중 1곳, 홍성군은 12곳 중 2곳, 당진시는 17곳 중 5곳이 P. brassicae에 오염된 것으로 나타났다. 토양내 P. brassicae에 의한 오염률 조사에서 해남군 30.3%, 영암군 및 영광군 38.5%, 고창군 16.7%, 홍성군 16.7%, 당진시 29.4%로 나타났다. 배추 뿌리혹이 육안으로 확인된 6곳은 PCR 검정에서 토양내 P. brassicae를 100% 확인할 수 있었다. 따라서 토양내 P. brassicae의 오염을 PCR 진단을 통해 화학적 방제여부를 결정하는 지표로 활용할 수 있을 것이다.

Keywords

References

  1. Buczacki, S. T. and Ockendon, J. G. 1979. Preliminary observations on variation in susceptibility to clubroot among collections of some wild Crucifers. Ann. Appl. Biol. 92: 113-118. https://doi.org/10.1111/j.1744-7348.1979.tb02963.x
  2. Choi, G. J., Jang, K. S., Kim, J. C., Lim, H. K., Chun, S. J., Kim, D. S. and Cho, K. Y. 2005. Control efficacy of ethaboxam on Chinese cabbage clubroot caused by Plasmodiophora brassicae. Korean J. Pestic. Sci. 9: 81-87. (In Korean)
  3. Dixon, G. R. 2009. The occurrence and economic impact of Plasmodiophora brassicae and clubroot disease. J. Plant Growth Regul. 28: 194-202. https://doi.org/10.1007/s00344-009-9090-y
  4. Dobson, R., Gabrielson, R. L. and Baker, A. S. 1982. Soil water matric potential requirements for root-hair and cortical infection of Chinese cabbage by Plasmodiophore brassicae. Phytopathology 72: 1598-1600. https://doi.org/10.1094/Phyto-72-1598
  5. Feng, J., Xiao, Q., Hwang, S. F., Stephen, E. S. and Bruce, D. G. 2012. Infection of canola by secondary zoospores of Plasmodiophora brassicae produced on a nonhost. Eur. J. Plant Pathol. 132: 309-315. https://doi.org/10.1007/s10658-011-9875-2
  6. Ingram, D. S. and Tommerup, L. C. 1972. The life history of Plasmodiophora brassicae Woron. Proc. R. Soc. Lond. B. 180: 103-112. https://doi.org/10.1098/rspb.1972.0008
  7. Ito, S., Ichinose, H., Yanagi, C., Tanaka, S. and Kameya-Iwaki, M. 2001. Identification of differentially expressed genes in susceptible and resistant responses of Chinese cabbage to Plasmodiophora brassicae. Phytopathology 149: 227-231. https://doi.org/10.1046/j.1439-0434.2001.00601.x
  8. Jang, S. J., Heo, S. H., Jang, C. S., Kang, S. W., Lim, Y. P. and Kim, H. G. 2007. Races and dominant population of Chinese cabbage clubroot pathogen Plasmodiphora brassicae in Korea. Res. Plant Dis. 13: 45-49. (In Korean) https://doi.org/10.5423/RPD.2007.13.1.045
  9. Jung, J. M., Yoon, B. K., Kim, H. J., Seo, Y. W., Yang, S. K. and Choi, K. J. 2007. The effects on resources of soil amendment matters friendly-environment on the marketing for agriculture of Chinese cabbage. J. Korean Bio-Environ. Cont. 16: 276-281.
  10. Kang, J. H., Kim, B. H., Lee, S. E., Kim, Y. J., Lee, J. W., Park, Y. M. and Ahn, S. C. 2005. Improved genomic DNA isolation from soil. J. Life Sci. 15: 851-856. https://doi.org/10.5352/JLS.2005.15.6.851
  11. Karling, J. S. 1968. The Plasmodiophorales. Hafner Publishing Company. New York. 256 pp.
  12. Kim, C. H., Cho, W. D. and Yang, J. M. 1999. Effects of temperature, soil moisture, soil pH and light on root gall development of Chinese cabbage by Plasmodiophora brssicae. Plant Dis. Agric. 5: 84-89.
  13. Kim, C. H., Cho, W. D. and Kim, H. M. 2000. Some environmental factors affecting decay of root galls in club root disease of Chinese cabbage. Korean J. Pestic. Sci. 4: 61-65.
  14. Kim, S. M., Choi, H. J., Kim, H. Y., Lee, D. K., Kim, T. H., Ahn, M. S. and Hur, J. H. 2002. Survey on pesticide use by Chinese cabbage growers in ganawon alpine farmland. Korean J. Pestic. Sci. 6: 250-256. (In Korean)
  15. Kim, C. H., Cho, W. D. and Lee, S. B. 2003. Review of researches on clubroot disease of Chinese cabbage in Korea and future tasks for its management. Res. Plant Dis. 9: 57-63. (In Korean) https://doi.org/10.5423/RPD.2003.9.2.057
  16. Lee, C. S. 2004. Actual condition survey of farms of summer Chinese cabbage on highland. Korean Soc. Soil & Sci. Fert. 18: 9-15. (In Korean)
  17. Lee, S. B. and Taylor, J. W. 1990. Isolation of DNA from fungal mycelia and single spores. In: PCR protocols, A guide to methods and applications, ed. by M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, pp. 282-287. Academic Press, Sandiego.
  18. Mattush, P. 1977. Epidemiology of clubroot of crucifers caused by Plasmodiophora brassicae. In: Woronin 100 International Conference on Clubroot, ed. by S. T. Buczacki and P. H. Williams, pp. 22-28, Univ. of Wiconsin, Madison, WI, USA.
  19. Mitani, S., Sugimoto, K., Hayashi, H., Takii, Y., Ohshima, T. and Matsuo, N. 2003. Effects of cyazofamid against Plasmodiophora brassicae Woronin on Chinese cabbage. Pest Manag. Sci. 59: 287-293. https://doi.org/10.1002/ps.627
  20. Park, S. Y., Yoon, M. K. and Lee, Y. P. 2011. Development of clubroot race4 resistant inbreds using conventional breeding and microspore culture method in Chinese cabbage. CNU J. Agri. Sci. 38: 613-618.
  21. Shimotori, H., Yanagida, H., Enomoto, Y., Igarashi, K., Yoshinari, M. and Umemoto, M. 1996. Evaluation of benzenesulfonanilide derivatives for the control of Crucifers clubroot. J. Pesticide Sci. 21: 31-35. https://doi.org/10.1584/jpestics.21.31
  22. Statistics Korea (KOSTAT). 2013. Korean agricultural statistics survey. http://kostat.go.kr
  23. Takahashi, K. 1994. Influences of some environmental factors on viability of resting spores of Plasmodiophore brassicae wor. incubated in sterile soil. Ann. Phytopath. SPC. Jpn. 60: 658-666. https://doi.org/10.3186/jjphytopath.60.658
  24. Vaughan, J. G. 1977. A multidisciplinary study of the taxonomy and origin of Brassica crops. Bioscience 27: 35-40. https://doi.org/10.2307/1297791
  25. Voorrips, R. E. 1996. Clubroot in the cole crops: the interaction between Plasmodiophora brassicae and Brassica oleracea. Thesis Wageningen.
  26. Yeates, C., Gillings, M. R., Davison, A. D., Altavilla, N. and Veal, D. A. 1998. Methods for microbial DNA extraction from soil for PCR amplification. Biol. Proced. Online 14: 140-147.
  27. Zhou, J., Bruns, M. A. and Tiedje, J. M. 1996. DNA recovery from soils of diverse composition. Appl. Environ. Microbiol. 62: 316-322.

Cited by

  1. Development of Species-Specific Primers for Plasmodiophora brassicae, Clubroot Pathogen of Kimchi Cabbage vol.20, pp.1, 2014, https://doi.org/10.5423/RPD.2014.20.1.021