• Journal of the korean academy of Pediatric Dentistry
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• v.25 no.2
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• pp.277-284
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• 1998

For the purpose of comparing the shear bond strengths of pit and fissure sealants, and finding out the more efficient method of tooth surface treatment when the etched surface is contaminaed by saliva or moisture, an experiment was performed on 3 types of pit and fissure sealants. 120 extracted human molars were divided into 3 groups, each of which was composed of 40 specimens sealed with Helioseal, Teethmate-F and Fuji III respectively. And each groups was again divided into 4 subgroups according to tooth surface treatment. The shear bond strengths of each groups and subgroup was measured and statistically analyzed. The results obtained were as follows : 1. Shear bond strengths of nonfluoridated resin sealant, Helioseal were shown to be higher than those of fluoridated resin sealant, Teethmate-F, but, not significantly different. 2. Shear bond strengths of GI sealant, Fuji III were to be markedly lower than those of two resin sealants. 3. When there is moisture contamination, applying primer under sealant(Group IV) results in a significantly stronger bond strength of sealant to enamel than when using sealant alone(Group II) in case of all sealants. 4. When there is no moisture contamination, using primer under sealant (Group III) results in bond strength equivalent to bond strength on using sealant alone (Group I). 5. Based on the results above, it was demonstrated that the bond of sealant to tooth surface is greatly affected by saliva contamination and that the complete tooth isolation method should be fully emphasized. The application of primer is recommended when performing sealant under the environment very susceptible to saliva contamination.

• Restorative Dentistry and Endodontics
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• v.17 no.2
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• pp.383-397
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• 1992

The purpose of this study was to evaluate the influence of artificial saliva contamination on bonding of several dentin adhesives to dentin. Sixty - three human molar teeth extracted within a month were used. Each tooth was sectioned longitudinally in a buccolingual direction to obtain 126 specimens. These specimens were randomly divided into three groups and were treated by Scotchbond 2, Gluma and All bond. Each group was subdivided into three subgroups; normal group not contaminated with artificial saliva, contaminated with artificial saliva and dried group, and contaminated with artificial saliva and washed and dried group. Enamel/dentin bonding agent(Dental Adhesive of Scotchbond 2) was applied and light cured on the treated dentin surfaces. Thereafter P - 50 were cured on them, and specimens were stored in $37^{\circ}C$ artificial saliva for 24 hours before measuring shear bond strength. Shear bond strengths were determined using an universal testing machine with cross head speed 1mm/min and SEM examinations were conducted to evaluate the resin - dentin interface and degree of penetrating resin string into the dentinal tubules. The following results were obtained. 1. Normal groups not contaminated with artificial saliva showed greater shear bond strength than any other group contaminated with artificial saliva(P<0.01). 2. The shear bond strengths showed no significant difference between washed groups with distilled water and not washed groups after contamination with artificial saliva(P>0.05). 3. In normal groups, the shear bond strength of A group was significantly greater than in any other group(P<0.01). 4. In Sand G groups, fractures after shear bond strength tests occured adhesively on resintooth interface in all specimens. But in A groups, fracture of the normal group occured cohesively in dentin and fracture of the contaminated groups occured adhesively and cohesively. 5. On SEM examination, the number of resin strings penetrated into dentinal tubules were the greatest in normal groups, followed by, in descending order, washed groups and not washed groups after contamination with artificial saliva.

• Tuberculosis and Respiratory Diseases
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• v.83 no.3
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• pp.211-217
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• 2020

The gold standard method for diagnosis of tuberculosis is the isolation of Mycobacterium tuberculosis through culture, but there is a probability of cross-contamination in simultaneous cultures of samples causing false-positives. This can result in delayed treatment of the underlying disease and drug side effects. In this paper, we reviewed studies on false-positive cultures of M. tuberculosis. Rate of occurrence, effective factors, and extent of false-positives were analyzed. Ways to identify and reduce the false-positives and management of them are critical for all laboratories. In most cases, false-positive is occurring in cases with only one positive culture but negative direct smear. The three most crucial factors in this regard are inappropriate technician function, contamination of reagents, and aerosol production. Thus, to reduce false-positives, good laboratory practice, as well as use of whole-genome sequencing or genotyping of all positive culture samples with a robust, extra pure method and rapid response, are essential for minimizing the rate of false-positives. Indeed, molecular approaches and epidemiological surveillance can provide a valuable tool besides culture to identify possible false positives.

• Proceedings of the Korean Geotechical Society Conference
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• 2009.09a
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• pp.1372-1377
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• 2009

For the remediation of the subsurface contaminated by nonaqueous phase liquids(NAPLs), it is important to characterize the NAPL zone properly. Conventional characterization methods provide data at discrete points. To overcome the weak points of conventional characterization methods, the partitioning tracer method has been developed and studied. The average saturation of NAPL($S_n$), which is the representative and continuous saturation value within contaminated site, can be calculated by comparing the transport of the partitioning tracers to that of the conservative tracer in the partitioning tracer method. In this study, the application of the partitioning tracer method in heterogeneous media was investigated. To represent the heterogeneous condition of subsurface, a two-dimensional soil box was divided into four layers and each layer contained different sized soils. Soils in the soil box were contaminated by the mixture of kerosene and diesel, and partitioning tracer tests were conducted before and after the contamination using methanol as conservative tracer and 4-methyl-2-pentanol, 2-ethyl-1-butanol, and hexanol as partitioning tracers. The response curves of partitioning tracers from contaminated soils were separated and retarded in comparison with those from non-contaminated soils. The contamination of soils by NAPLs, therefore, can be detected by partitioning tracer method considering these retardations of tracers. From our experiment condition, the average saturation of NAPLs calculated by partitioning tracer method using the methanol as conservative tracer and hexanol as partitioning tracer showed the highest accuracy, though all results were underestimated. Further studies, therefore, were needed for improving the accuracy using the partitioning tracer test in heterogeneous media.

• Korean Chemical Engineering Research
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• v.50 no.2
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• pp.187-190
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• 2012

Contamination of ion from cathode air on the membrane and electrode assembly (MEA) is the serious degradation source in proton exchange membrane fuel cells (PEMFC). In this study, concentration of ions in air at industry region, street and seaside were measured. There were comparably high concentration of $Na^+$, $K^+$, $Ca^{2+}$ and $Fe^{3+}$ in this regions. This paper shows the effects of MEA contamination by these ions generated from humidification water. After 170 hours of fuel cell operation using city water as humidification water, the performance of unit cell decrease to 11% of initial performance. The electrolyte membrane easily absorbed foreign contaminant cations due to the stronger affinity of foreign cations with the sulfonic acid group compared to $H^+$. The contaminant ions existing in the interface between the platinum catalyst and ionomer layer turn out to be the most serious factor to decrease cell performance.

• Genomics & Informatics
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• v.7 no.1
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• pp.32-37
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• 2009

The quality assurance (QA) is of utmost importance in biobanks when archived biomaterials are distributed to biomedical researchers. For sample authentication and cross-contamination detection, the two fundamental elements of QA, STR genotyping is usually utilized. However, the incorporated number of STR markers is highly redundant for biobanking purposes, resulting in time and cost inefficiency. An index to measure the cross-contamination detection capability of an STR marker, the mixture probability (MP), was developed. MP as well as other forensic parameters for STR markers was validated using STR genotyping data on 2328 normal Koreans with the commercial AmpFlSTR kit. For Koreans, 7 STR marker (D2S1338, FGA, D18S51, D8S1179, D13S317, D21S11, vWA) set was sufficient to provide discrimination power of ${\sim}10^{-10}$ and cross-contamination detection probability of ${sim}1$. Interestingly, similar marker sets were obtained from African Americans, Caucasian Americans, and Hispanic Americans under the same level of discrimination power. Only a small subset of commonly used STR markers is sufficient for QA purposes in biobanks. A procedure for selecting optimal STR markers is outlined using STR genotyping results from normal Korean population.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.27 no.6
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• pp.523-527
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• 2005

Allograft donations are commonly found to be contaminated. The most of tissue banks has promoted the use of ionizing radiation for the sterilization of biological tissues. The potential for transmission of human infectious diseases and contamination of microorganism has created serious concern for the continued clinical use of hard and soft-tissue allografts. Tissue banks have employed 15-25kGy for sterilization of hard and tendon allografts, which, according to the national standards, approaches the level at which the tissue quality is adversely affected for transplantation. The donations of allogeneic tissues to the Korea Tissue Bank over a 2-year period were reviewed, and the incidence and bacteriology of contamination were detailed. Clinical outcomes were determined for donors who had positive cultures at the time of retrieval and during the processing and they were compared with those of post sterilization. After exposure of the frozen block bone to 25kGy and the processed tissues to 15kGy of gamma irradiation, the authors were able to demonstrate complete inactivation of the bacteria. The aim of this study was to obtain the effects of gamma irradiation and the irradiation dose according to the type of tissue, through conventional microbiologic test without on influence of biocompatibility in allografts. The contamination rate after the final irradiation sterilization is 0% in the processed allografts. This may be due to the fact that the gamma radiation and processing steps are effective to control contamination.

• Journal of the East Asian Society of Dietary Life
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• v.24 no.4
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• pp.481-487
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• 2014

This study examined microbiological contamination of street foods(kimbab, fish cake, Korean sausage) by microbiological analysis. A collection of 360 samples of street foods was obtained seasonally in four major cities(Seoul, Daejeon, Busan, Gwangju) in Korea. Aerobic mesophilic counts ranged between 1.0 and 9.9 log CFU/g, with the highest count recorded from Kimbab. Counts of psychrotrophic microorganisms were as high as those of mesophilic microorganisms. Total coliform populations between <1.0 and 7.5 log CFU/g were found in 53.6% of samples. Escherichia coli 4.4%, Staphylococcus aureus 7.8% and Clostridium perfringens 3.3%. Salmonella spp. and Listeria monocytogenes were not detected in any of the samples. Kimbab purchased in spring and summer showed higher S. aureus and Cl. perfringens contamination rates. Microbial contamination levels determined in the present study may be used as the primary data to execute microbial risk assessment of street foods.

• Nuclear Engineering and Technology
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• v.2 no.2
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• pp.97-106
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• 1970

Radiation accidents which occured in the A.E.R.I. during last ten years are described (table 1). It seemed to the authors that some of these accidents were considered to be hazardous to man body and associated installations. This report deals with the following four major accidents involving body contamination incidents that our health physicists have been experienced. 1. Over-exposures (up to 130 rem) to the total body due to the mismanipulation in the Cobalt-60 gamma irradiation facility. 2. Floor surface contamination (up to 13 mrad/hr) and its spread out due to the mishandling of radioiodine contained in the bottle. 3. Body surface contamination and 0.36 uCi radioactivity accumulated in the thyroid gland of a worker due to the inhalation of gaseous iodine-131. 4. A void capsule due to the leakage out of the radium therapeutic source (3mg\ulcorner) These accidents were treated by definitely prompt action to protect the workers and associated installations from any radiation hazards and every possible efforts were made to confine the spread of radioactive contamination as small area as possible by means of elaborate decontamination work and monitoring.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.24 no.4
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• pp.297-305
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• 2012

In recent large-scale semiconductor manufacturing clean rooms, the energy consumption of outdoor air conditioning systems to heat, humidify, cool and dehumidify incoming outdoor air represents about 45% of the total air conditioning load required to maintain a clean room environment. Therefore, the energy performance evaluation and analysis of outdoor air conditioning systems is useful for reducing the outdoor air conditioning load for a clean room. In the present study, an experiment was conducted to compare the energy consumption of outdoor air conditioning systems with a simple air washer, an exhaust air heat recovery type air washer and a DCC return water heat recovery type air washer. It was shown from the present lab-scale experiment with an outdoor air flow of 1,000 $m^3/h$ that the exhaust air heat recovery type and DCC return water heat recovery type air washer outdoor air conditioning systems were more energy-efficient for the summer and winter operations than the simple air washer outdoor air conditioning system and furthermore, the DCC return water heat recovery type one was the most energy-efficient in the winter operation.