• Journal of Microbiology and Biotechnology
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• 제29권1호
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• pp.55-58
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• 2019

Development of stable rCHO cell lines is still time consuming and labor intensive, although it is a critical step in the commercial development of recombinant antibodies. The current work demonstrates, for the first time, that electroporation of CHO cells with DMSO can enhance stable expression of recombinant antibodies in rCHO cells. Electroporation with DMSO resulted in an average 3.7-fold and 2.8-fold increases in expression levels of aflibercept and pembrolizumab, respectively, in pools of stable rCHO cells. It also resulted in an average of 2.2-fold and 2.6-fold increases in the expression of aflibercept and pembrolizumab, respectively, in single-cell derived rCHO clones. Simple batch cultures of rCHO cell clones with the highest expression produced 1.0 g/l for aflibercept and 1.4 g/l for pembrolizumab without a time-consuming gene amplification process. Electroporation with DMSO also shortened the development of rCHO cell lines to 2-3 months, allowing rapid establishment of stable rCHO cell lines with a desirable expression level antibodies.

• 로봇학회논문지
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• 제19권1호
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• pp.65-70
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• 2024

Tool wear is considered an important issue in manufacturing and engineering, as worn tools can negatively impact productivity and product quality. Given that the wear status of tools plays a decisive role in the production process, measuring tool wear is a key task. Consequently, there is significant attention in manufacturing fields on the precise measurement of tool wear. Current domestic methods for measuring wear are limited in terms of speed and efficiency, with traditional methods being time-consuming and reliant on subjective evaluation. To address these issues, we developed a measurement module implementing the DeepContour algorithm, which uses image processing technology for rapid measurement and evaluation of tool wear. This algorithm accurately extracts the tool's outline, assesses its condition, determines the degree of wear, and proves more efficient than existing, subjective, and time-consuming methods. The main objective of this paper is to design and apply in practice an algorithm and measurement module that can measure and evaluate tool wear using image processing technology. It focuses on determining the degree of wear by extracting the tool's outline, assessing its condition, and presenting the measured value to the operator.

• 전자공학회논문지
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• 제54권2호
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• pp.53-57
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• 2017

본 논문에서는 의료 기기용 401MHz - 406MHz MedRadio 대역에서 사용하는 저잡음 증폭기를 제안한다. 제안한 저잡음 증폭기는 전류 재사용 gm-boosting 기술을 이용한 공통 게이트 증폭기 구조를 채택하여 기존의 gm-boosted 공통 게이트 증폭기에 비해 동일한 전력소모에서 더 높은 전압 이득과 더 낮은 잡음 지수 특성을 얻었다. 제안한 전류 재사용 gm-boosted 저잡음 증폭기는 $0.13{\mu}m$ CMOS 공정을 사용하여 설계하였고, 22 dB의 전압 이득, 2.95 dB의 잡음 지수, -17 dBm의 IIP3 특성을 보이며, 공급 전압 0.5 V에서 $170{\mu}W$의 전력을 소비한다.

• 한국식품영양과학회지
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• 제28권3호
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• pp.586-592
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• 1999

Microwave assisted extraction(MAE) is known as a more environmental friendly process with economic advantages in terms of less time, less solvent, less energy and less wastes than the current time consuming reflux method. It was applied to develop a rapid extraction method for soluble ginseng components that are major materials used for the processing of ginseng products. In a comparative study between pre established MAE(ethanol 60%, power 80 W, process time 4 min$\times$5) and current extraction method(ethanol 80%, temp. 85oC, time 8 hr$\times$5), MAE was more efficient than the current method to obtain an extract yield(soluble solid), but it was insufficient to extract individual ginsenosides, total phenols, reducing components and acidic polysaccharides. MAE with 80% ethanol by 5 times showed, however, that its extraction efficiency on soluble solid, crude saponin, major ginsenosides, and the other components was equal or superior to that of the current method, indicating that ethanol concentration is one of the critical parameters influencing the MAE process. The quality of ginseng extracts from MAE was assured by evaluating the corresponding standards and by comparing TLC and HPLC patterns with the control.

• Journal of Power Electronics
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• 제15권4호
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• pp.1139-1147
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• 2015

In this paper, an algorithm based on a model analysis of the online calculation of the root-mean-square (RMS) value of welding current for single-phase AC resistance spot welding (RSW) was developed. The current is highly nonlinear and typically non-sinusoidal, which makes the measuring and controlling actions difficult. Though some previous methods focused on this issue, they were so complex that they could not be effectively used in general cases. The electrical model of a single-phase AC RSW was analyzed, and then an algorithm for online calculation of the RMS value of the welding current was presented. The description includes two parts, a model-dependent part and a model-independent part. Using a previous work about online measurement of the power factor angle, the first part can be solved. For the second part, although the solution of the governing equation can be directly obtained, a lot of CPU time must be consumed due to the fact that it involves a lot of complex calculations. Therefore, a neural network was employed to simplify the calculations. Finally, experimental results and a corresponding analysis showed that the proposed algorithm can obtain the RMS values with a high precision while consuming less time when compared to directly solving the equations.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회B
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• pp.3365-3370
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• 2007

We developed micro power generation system using piezoelectric materials. In our system, the ambient vibrating energy is converting to electric energy by deflection of piezoelectric beams. The system consists of energy generating parts, converting enhancement parts, electric regulation and charging parts, and interface with small-energy-consuming mobile devices. The geometry of piezoelectric beams, the source of vibrating energy, and the electric load of target application determine the characteristics of generating electric power, such as impedance, voltage, current and power density. Therefore, we made a model for analysis of generating power with given information such as piezoelectric materials, geometry, vibration type, and mass. With this model, we can calculate capacitance of piezoelectric beams, generating voltage, current, and power. To obtain maximum energy transfer efficiency, we approached this study in the view of material, electrical, and mechanical engineering

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2001년도 제14회 신호처리 합동 학술대회 논문집
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• pp.121-124
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• 2001

For efficient transmission of 3DTV video signals, it is necessary to eliminate the inherent redundancy between the stereo image pairs. Though disparity estimation provides a powerful tool for eliminating the redundancy, it is very time consuming. This paper presents a novel disparity estimation scheme based on the human visual property. The disparity vectors of image blocks spatially adjacent to the current block are used as initial guesses fur the disparity vector of the current block. In addition, mixed-resolution coding is applied to reduce the computational complexity of disparity estimation. Through computer simulations on a stereoscopic sequence we show that the proposed method gives rise .to visually pleasing results with much reduced computational complexity.

• 한국IT서비스학회지
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• 제4권1호
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• pp.141-148
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• 2005

The importance of education is greatly emphasized in the IT era. However, teachers have to deal with teaching work as well as increasing non-educationalchores. One of those chores is student counseling. Nowadays, teachers have considerable difficulty in consulting students because they cannot visit their home and don't have enough information on students. Furthermore, students are not willing to participate in school counseling since they think school counseling is not of much help and is just time-consuming. Most of the current school counseling is focused on matters related to entrance into a school of higher grade such as choice of college. But, students need counseling on matters regarding their ordinary life. This paper suggests a web-based student-counseling system that may solve this problem. The paper identifies core requirements for the web-based student-counseling system, discusses its design and implementation and shows it is more effective than the current ordinary school counseling by experimenting on a group of students with this system.

• 대한기계학회논문집A
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• 제25권4호
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• pp.610-618
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• 2001

Pantograph, which collects current from cartenary system, is one of the important parts of high-speed train. Kinematic analysis is one of the key technologies for pantograph design and includes joint trajectories, reaction forces, and the required moment of main shaft calculations. The kinematic analysis, however, is very complex and time-consuming process. In this study PC based pantograph kinematic analysis software using graphical user interface tool was developed for the easy evaluations of kinematic characteristics necessary in pantograph design process.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 1995년도 춘계학술대회논문집
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• pp.224-232
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• 1995

A process is designed by the backward tracing scheme of the rigid-plastic FEm for net-shape shell nosing component without machining after forming. The current process of the shell nosing industry requires cost-consuming machining to produce final product . The backward tracing scheme of the rigid-plastic FEM, a novel method in preform design of metal forming processes , derives a sound preform for net-shape shell nosing product . The current process is simulated to check the metal flow involved informing with a trial preform and its modified preform. It is found that the two preforms are not suitable for net-shape shell nosing product. Finally, a preform is desinged by the backward tracing scheme, which is suitable for net-shape manufacturing of the shell nosing component.