• Journal of Microbiology and Biotechnology
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• 제26권12호
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• pp.2184-2191
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• 2016

The time-consuming and high-cost preparation of soluble peptide-major histocompatibility complexes (pMHC) currently limits their wide uses in monitoring antigen-specific T cells. The single-chain trimer (SCT) of peptide-${\beta}2m$-MHC class I heavy chain was developed as an alternative strategy, but its gene fusion is hindered in many cases owing to the incompatibility between the multiple restriction enzymes and the restriction endonuclease sites of plasmid vectors. In this study, overlap extension PCR and one-step cloning were adopted to overcome this restriction. The SCT gene of the $OVA_{257-264}$ peptide-$(GS_4)_3-{\beta}2m-(GS_4)_4-H-2K^b$ heavy chain was constructed and inserted into plasmid pET28a by overlap extension PCR and one-step cloning, without the requirement of restriction enzymes. The SCT protein was expressed in Escherichia coli, and then purified and refolded. The resulting $H-2K^b/OVA_{257-264}$ complex showed the correct structural conformation and capability to bind with $OVA_{257-264}$-specific T-cell receptor. The overlap extension PCR and one-step cloning ensure the construction of single-chain MHC class I molecules associated with random epitopes, and will facilitate the preparation of soluble pMHC multimers.

• 비교문화연구
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• 제39권
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• pp.329-354
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• 2015

The major goals of this paper are to identify the degree into which the meanings of 'see' verb can be extended, focusing on the extended meanings shown in the expressions that denote our instinctive actions for survival, such as eating or drinking, etc., and to clarify the doubt on whether any syntactic pattern can be associated with the meaning in the process of meaning extension of 'see' verb. For doing this task, this paper picked out 2,000 examples randomly from COCA (Corpus of Contemporary American English), in which the verb 'see' is used. This paper classified the sentences into thirteen different sentence types, according to the syntactic patterns. This research showed that these thirteen syntactic types lead us to figure out the process of the meaning extension of the verb 'see'. With this result, this paper made an attempt to provide the four steps toward the meaning extension of verb 'see'. The verb 'see' in the first step denotes the meaning of purely seeing the visualized objects. This verb in the second step expresses the shifted function, under which the agent in the subject position takes the seeing action as a secondary task in order to carry out other main task. The verb in the third step denotes the extended meanings irrelevant to the seeing action, because the sentences on this step do not contain any visualized objects. In the last step this verb functions as conventional implicature whose meaning does not contribute to the whole meaning of a sentence. In addition, this paper identified that the syntactic properties are deeply associated with the process of meaning extension of the verb 'see', and tried to formalize this relationship between the syntax and semantics within the framework of Construction Grammar based on A. Goldberg.

• 한국강구조학회 논문집
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• 제9권2호통권31호
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• pp.229-235
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• 1997

임의항복강도가 강구조물의 에너지소산능력에 미치는 영향을 파악하기 위해 본 논문에서는 7개의 강뼈대구조물을 모델링하여 응답스펙트럼해석법에 적용되는 거동계수를 산출하고 그 분포상태를 결정하였다. 또한 지진하중의 임의성이 거동계수에 미치는 영향과 비교하기 위해 주어진 스펙트럼을 만족하는 4개의 인공지진을 시뮬레이션하여 적용하였다. 본 연구의 특성상 방대한 양의 시간-이력계산을 수행하여야 하므로 근사해법인 시간-이력해석법을 개발하여 신뢰도를 검토하고 적용하였다.

• 전자공학회논문지
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• 제51권10호
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• pp.248-255
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• 2014

본 논문에서는 다양한 특징점들을 이용하여 아스팔트 도로의 포트홀을 검출하는 방법을 제안한다. 포트홀 검출에서의 분할, 후보, 결정 단계 들은 특징점 들의 특성에 따라 추출된 값들에 의해 처리된다. 분할 단계에서는 히스토그램과 형태학 필터의 닫힘 연산을 이용하여 포트홀 검출을 위한 어두운 영역을 추출한다. 후보 단계에서는 포트홀 후보 영역을 정하기 위하여 크기, 밀도 등 다양한 특징점들을 이용하여 포트홀 후보 영역을 추출한다. 또한 마지막 결정 단계에서는 후보 영역과 배경 영역과의 특징점들의 비교를 통해서 후보 영역이 포트홀 여부를 판단한다. 실험 결과, 제안하는 방법이 기존 포트홀 검출 방법 보다 향상된 결과를 보이고 포트홀과 유사한 형태들과 구분하는 향상된 결과를 보인다.

• 환경영향평가
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• 제18권1호
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• pp.11-19
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• 2009

We conducted human risk assessment due to inhalation exposure to heavy metals emitted from incinerator. The process of health impact assessment(HIA) on incinerator is as follows: The first step is to presume and calculate the amount of heavy metals emitted using emission factor. The second step is to conduct an exposure assessment using the K-SCREEN model which is used for predicting the concentration in a conservative method. The last step is to carry out a risk assessment on carcinogenic and non-carcinogenic substances. This study revealed that rank of carcinogenic human risk was $Cr^{+6}$ > As > Ni > Cd, and values of human risk assessment on carcinogenic and non-carcinogenic substances is lower than the US criteria for risk assessment except $Cr^{+6}$. It is expected that the technique of HIA, especially human risk assessment on heavy metals, would be applied to the incinerator construction project. In addition, more systematic studies are needed to overcome some weak points and limits found in this study.

• Geomechanics and Engineering
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• 제28권6호
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• pp.573-584
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• 2022

Water inrush may occur during seaside urban tunnel excavation. Various factors affect the water inrush, and the water inrush mechanism is complex. In this study, nine evaluation indices having potential effects on water inrush were analysed. Specifically, the geographic and geomorphic conditions, unfavourable geology, distance from the tunnel to sea, strength of the surrounding rock, groundwater level, tidal action, cyclical footage, grouting pressure, and grouting reinforced region were analysed. Furthermore, a two-step interval risk assessment method for water inrush management during seaside urban tunnel excavation was developed by a multi-index system and interval risk assessment comprised of an interval analytic hierarchy process, fuzzy comprehensive evaluation, and relative superiority analysis. The novel assessment method was applied to the Haicang Tunnel successfully. A preliminary interval risk assessment method for water inrush was performed based on engineering geological conditions. As a result, the risk level fell into a risk level IV, which represents a section with high risk. Subsequently, a secondary interval risk assessment method was performed based on engineering geological conditions and construction conditions. The risk level of water inrush is reduced to a risk level II. The results agreed with the current tunnel situation, which verified the reliability of this approach.

• 한국건축시공학회지
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• 제8권5호
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• pp.75-83
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• 2008

Larger and more sophisticated building construction requires more input resources such as worker, materials and devices. Growing resource volume brings risks at a construction site. The industry makes an effort to protect probable incidents at the site by organizing a safety management team. conducting a safety instruction and etc, but losses especially in the construction are higher than other industries. Major reason is that the safety management program is conducted only at the step of construction work and a root cause is not eliminated. Conventionally a concerned party shifts the blame to other parties such as constructor and site workers who are direct participants in the construction site. However, the whole causes of incidents go to the all subject of the construction not only the constructor but the client, designer and others related in the construction, and especially the clients are heavily involved in general concerns of the project. Therefore, this study is defined the role of the clients in nations and domestic condition of construction safety management is investigated. And it is analyzed surveys to prevent incidents at construction sites, and suggested the role of the clients which is classified pre and post construction, and in the middle of construction, and also categorized planning and design & construction schedule especially for the pre-construction level.

• 한국건축시공학회지
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• 제12권5호
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• pp.528-538
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• 2012

With domestic construction projects becoming bigger, more specialized and more advanced, the construction industry is striving to improve quality and quantity, and is diversifying functions and shapes. Nevertheless, the process of a construction project causes problems when we estimate construction price, because the cost breakdown structures are different in each step. The primary aim of this study was to estimate building life cycle cost using the Delphi method. The cost breakdown structure for life cycle cost was classified into planning, design, construction, maintenance and waste disposal, and each detailed classification was determined by estimating life cycle cost. Moreover, the developed cost breakdown structure is verified by consulting with experts to secure objectivity and validity.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2015년도 춘계 학술논문 발표대회
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• pp.119-120
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• 2015

Appropriate management of the finish works in construction project is one of most important activities that must be conducted considering the total duration of the project. Masonry work is a fundamental process that is performed in the preliminary steps of finish works. However, it has been investigated that the analysis of delay factors affecting masonry work has been neglected in the domestic construction site. Therefore, this study deducts delay factors affecting masonry work by literature review and survey on site engineers and labors. This study has been conducted as a preliminary step of developing a construction project interference management system which is expected to suggest objective information for the decision making in construction sites.

• 대한안전경영과학회지
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• 제5권3호
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• pp.45-55
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• 2003

Construction Management(CM) system, which was introduced some years ago to large national construction projects, such as the construction of Incheon New Airport, construction of high-speed railroad and World Cup stadiums, is considered to be still in its early stage and safety at each step of CM is very poorly controlled. In an attempt to improve such inferior safety control of domestic CM, this study analyzed safety control operation system in CM home and abroad and suggested a model for the construction of safety control management information system suitable to the conditions of Korea.