• Journal of the Korean Institute of Rural Architecture
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• v.22 no.3
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• pp.17-24
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• 2020

Reservoirs, which make up most of South Korea's reservoirs, are located in rural areas. In the case of rural reservoirs, about 75% have been reported over 50 years old aged reservoirs constructed before the 1960s. Reservoirs are important facilities that store and supply water necessary for daily life. However, if it is destroyed, the reservoir can cause a lot of damage, so continuous management is necessary. As a method for strengthening old reservoirs, a method using cement has been widely applied. However, OPC is a product that uses a lot of carbon dioxide and natural resources. Therefore, the amount of cement should be reduced. Against this background, in this study, the ground injection material of utilizing circulation resources was applied to the site. Applied reservoirs have been around for 75 years and leaks have occurred in some sections. The application method was constructed in two rows, up to a depth of 12m. After reinforcement, the electrical resistivity test was conducted three times. As a result, similar resistance was shown at 1 month after construction. And after 6 months, the saturation area decreased. And the performance after reinforcing the aged reservoir was examined. As a result of the review, this study confirmed that the applicability was equivalent to that of OPC, and the excellent performance of reinforcing the aged reservoir was shown.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.10 no.10
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• pp.1773-1778
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• 2006

This paper propose the method of constructing the Digital Logic Systems based on the Improved Automatic Theorem Proving Techniques(IATP) over Finite Fields. The proposed method is as following. First, we discuss the background and the important mathematical properties for Finite Fields. Also, we discuss the concepts of the Automatic Theorem Proving Techniques(ATP) including the syntactic method and semantic method, and discuss the basic properties for the Alf. In this step, we define several definitions of the IAIP, Table Pseudo Function Tab and Equal. Next, we propose the T-gate as Building Block(BB) and describe the mathematical representation for the notation of T-gate. Then we discuss the important properties for the T-gate. Also, we propose the several relationships that are Identity relationship, Constant relationship, Tautology relationship and Mod R cyclic relationship. Then we propose Mod R negation gate and the manipulation of the don't care conditions. Finally, we propose the algorithm for the constructing the method of the digital logic systems over finite fields. The proposed method is more efficiency and regularity than my other earlier methods. Thet we prospect the future research and prospects.

• Journal of the Korean Wood Science and Technology
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• v.46 no.6
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• pp.735-747
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• 2018

Radon radiation exposures in home have been posed as a potential cancer hazard. This research aims to present the basic data of the indoor radon concentration level by examining the radon exhalation rates of wood species. Radon exhalation rates from five commonly used wood species in Korean wood building construction were measured with Continuous Radon Monitor (CRM), Model 1028 (Sun Nuclear Co., USA) using the Closed Chamber Method (CCM). The mass exhalation rate was observed to vary from $0.00089Bq{\cdot}kg^{-1}{\cdot}h^{-1}$ to $0.00181Bq{\cdot}kg^{-1}{\cdot}h^{-1}$, whereas the surface exhalation rate was observed to be $0.00677-0.01517Bq{\cdot}m^{-2}{\cdot}h^{-1}$. The radon exhalation rate of Quercus accutissima Carruth (white oak) which has the highest density showed the highest figure among the five wood species, on the other hand, the rest of four species showed similar results which were similar to the radon exhalation rates of wood in the U.S.A. and Canada. The average of the concentration measured by the CCM represented well up to the second half-life period (7.7 days). Because result of these small quantities seems to indicate that radon exhalation from the tested wood species has almost negligible impact, the main culprit of the high indoor radon concentration is clearly derived from the background of surrounding wood house. Therefore, as a safety precaution, infrastructures made of wood materials should be designed with the consideration of influx of radon and built accordingly. Furthermore, it is highly desirable that wood will be needed to use for furniture and interior finishing material in indoor environment.

• Korean Institute of Interior Design Journal
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• v.22 no.2
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• pp.176-185
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• 2013

(Background)In modern industrial society, the design industry failed to observe the law of nature, destructing it. Regardless its intention, the design industry destructed the environment so that it can't maintain the future life because of waste and disaster. For the purpose, it is important to adopt the technology to reuse the waste resource generated by building or minimize the damage to environment for the resource that can't be recycled. (Methods)On the assumption that the material and resource can be an alternative plan for the design that can make environment be sustained, the study analyzed materials and resources out of superior office space of USA, which were selected by LEED Green Building Rating System. (Results)The analysis result revealed that all cases reused main structural part of existing building and indoor and various materials were reused or recycled. Especially, the materials without or with low amount of VOCs and formaldehyde were used. In order to reduce construction waste, the finish of existing building was exposed as it was, 50% of reused materials were used or disassemblable materials were used. When regional materials are used, there is an advantage to reduce transportation cost and recycle the materials rapidly. Lastly, the environment-friendly certified by FSC was used in all cases. (Conclusion)After all, the material is one of the space design strategies sensitive to environment so it is important to select good material. Harmless, environment-friendly materials applied to sustainable office space contribute to the creation of healthy environment. In addition, the use of recycled materials and reused materials to minimize waste is also essential factor for creating sustainable space.

• Journal of Microbiology and Biotechnology
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• v.20 no.1
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• pp.45-53
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• 2010

We have developed an efficient and precise method for genome manipulations in Bacillus subtilis that allows rapid alteration of a gene sequence or multiple gene sequences without altering the chromosome in any other way. In our approach, the Escherichia coli toxin gene mazF, which was used as a counter-selectable marker, was placed under the control of a xylose-inducible expression system and associated with an antibiotic resistance gene to create a "mazF-cassette". A polymerase chain reaction (PCR)-generated fragment, consisting of two homology regions joined to the mazF-cassette, was integrated into the chromosome at the target locus by homologous recombination, using positive selection for antibiotic resistance. Then, the excision of the mazF-cassette from the chromosome by a single-crossover event between two short directly repeated (DR) sequences, included in the design of the PCR products, was achieved by counter-selection of mazF. We used this method efficiently and precisely to deliver a point mutation, to inactivate a specific gene, to delete a large genomic region, and to generate the in-frame deletion with minimal polar effects in the same background.

• Reproductive and Developmental Biology
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• v.33 no.1
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• pp.63-69
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• 2009

In this study we tried to construct a more efficient tetracycline-inducible gene expression system by replacing previous key elements with more advance ones. At the beginning, we substituted PGK (phophoglycerate kinase) promoter for CMV (cytomegalovirus) promoter to control "$rtTA2^sM2$" which has been known for high induction efficiency in response to tetracycline. With this modification, expression of the EGFP marker gene under the induction condition was significantly increased. Next, we replaced "TRE" fragment with a modified version named "TRE-tighf" which has been reported to have higher affinity and specificity to the transactivator by minor base change of the "TRE" DNA fragment sequence. Use of "TRE-tighf" instead of "TRE" resulted in more than 10 fold increment in terms of induction efficiency and significant decrement of background expression in non-inducible condition. By combining PGK promoter and "TRE-tight" fragment, we could upgrade previous tetracycline-inducible system to show more stringent turn on/off gene switch ability and stronger expression of the gene of our interest. Use of this newly developed system must be very helpful to the studies of gene expression, especially to the transgenic animal study in which non-controllable constitutive expression of the transgene has been one of the urgent problems to be solved.

• Geomechanics and Engineering
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• v.14 no.5
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• pp.407-419
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• 2018

In order to investigate flow characteristics after water inrush from the working face in process of karst tunnel construction, numerical calculation for two class case studies of water inrush is carried out by using the FLUENT software on the background of Qiyueshan tunnel. For each class water inrush from the tunnel face, five cases under different water-inrush velocity are simulated and researched. Three probing lines are selected respectively in the left tunnel, cross passage, right tunnel and in the height direction of the tunnel centerline. The variation characteristics of velocity and pressure on each probing line under the five water-inrush velocities are analyzed. As for the selected four groups probing lines in the tunnels, the change rules of velocity and pressure on each group probing lines under the same water-inrush velocity are discussed. Finally, the water flow characteristics after inrush from the tunnel face are summarized by comparing the case studies. The results indicate that: (1) The velocity and pressure change greatly at the intersection area of the cross passage and the tunnels. (2) The velocity nearby the tunnel side wall is the minimum, while it is the maximum in the middle position. (3) The pressure value of every cross section in the tunnels is basically fixed. (4) As water-inrush velocity increases, the flow velocity and pressure in the tunnels also increase. The former is approximately proportional to their respective water-inrush velocity, while the latter is not. The research results provide a theoretical basis for making scientific and rational escape routes.

• Journal of Microbiology and Biotechnology
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• v.25 no.10
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• pp.1599-1605
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• 2015

The development of rapid and efficient genome sequencing methods has enabled us to study the evolutionary background of bacterial genetic information. Here, we present comparative genomic analysis of 17 Streptomyces species, for which the genome has been completely sequenced, using the pan-genome approach. The analysis revealed that 34,592 ortholog clusters constituted the pan-genome of these Streptomyces species, including 2,018 in the core genome, 11,743 in the dispensable genome, and 20,831 in the unique genome. The core genome was converged to a smaller number of genes than reported previously, with 3,096 gene families. Functional enrichment analysis showed that genes involved in transcription were most abundant in the Streptomyces pan-genome. Finally, we investigated core genes for the sigma factors, mycothiol biosynthesis pathway, and secondary metabolism pathways; our data showed that many genes involved in stress response and morphological differentiation were commonly expressed in Streptomyces species. Elucidation of the core genome offers a basis for understanding the functional evolution of Streptomyces species and provides insights into target selection for the construction of industrial strains.

• Journal of the Korean Society for information Management
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• v.37 no.3
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• pp.107-131
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• 2020

This study is to examine the possibility of applying the Article 35-4 of the Copyright Act and the relevant regulations of the enforcement decree of the same law to the construction and service of digital library. The background and necessity of the revision were understood by examining the related regulations and discussions in previous studies, including Articles 31 and 50 of the Copyright Act. Then, the detailed contents of the provision were analyzed by referring to the laws, enforcement decrees, and ordinance of the Ministry of Culture, Sports and Tourism. In addition, through comparison with Articles 31 and 50 of the Copyright Act, the characteristics, significance, limitations, and problems of this provisions were analyzed. Based on this, we proposed four ways to increase the effectiveness of this provision.

• KIEAE Journal
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• v.16 no.1
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• pp.121-130
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• 2016

Purpose: This paper is an attempt to explicate the principle of space and form-making in Le Corbusier's architecture from the viewpoint of integrating and relating architecture with the natural landscape. It shows thereby how the geometrical element of architecture and the non-geometrical element of the nature coexist in terms of form. It also explains how space is deployed for the construction of cognitive system between natural landscape and architectural scenery in terms of space, hoping to make a contribution to design method of contemporary house as an elementary datum. Method: Three issues are studied, focusing on the designs around nineteenth thirties in the earliest phase of Le Corbusier: How architectural form and nature is related, how natural greens is introduced into urbanism, and how natural landscape is introduced to house design. 1) it is studied how the relation between the nature and the formal elements of purism in Le Corbusier's architecture is formed. 2) it is studied how the relation between the nature and architecture proposed in Le Corbusier's urbanism is formed. 3) Le Corbusier's "four compositions" is analyzed in terms of relation-formation between architectural space and the nature. 4) it is studied how the natural landscape is introduced to Le Corbusier's house-design in the form of architectural promenade. Result: It has been found out that the natural landscape in Le Corbusier's architecture is not just a simple background, but a necessary element for the consummation of form and space in his architecture, and that architectural inspiration is maximized with coexistence with nature.